LOCUS       sequence-25928-e         806 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector for Firefly Luciferase shRNA under H1/TO 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pENTR/pTER shLUC (w177-1)
FEATURES             Location/Qualifiers
     source          1..806
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    17..35
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    complement(168..267)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(379..398)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(457..476)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 ttgggatctt ataagttccc tatcagtgat agagatcccc ttacgctgag tacttcgagt
       61 gtgctgtcct cgaagtactc agcgtaagtt tttggaaaag cttggtaccg agctcggatc
      121 cactagtcca gtgtggtgga attcgcggcc gcactcgaga tatctagacc cagctttctt
      181 gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac
      241 tatcagtcaa aataaaatca ttatttgcca tccagctgca gctctggccc gtgtctcaaa
      301 atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg
      361 cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcgaggc
      421 cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg
      481 tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg ccagagttgt
      541 ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa
      601 actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg
      661 atgcatggtt actcaccact gcgatccccg gaaaaacagc attccaggta ttagaagaat
      721 atcctgattc aggtgaaaat attgttgatg cgctggcagt gtccctgcgc cggttgcatt
      781 cgattcctgt ttgtaattgt cctttt
//
