LOCUS       sequence-259280-        8943 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Myr mCherryDrTrkB expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8943)
  AUTHORS   Leopold AV, Chernov KG, Shemetov AA, Verkhusha VV
  TITLE     Neurotrophin receptor tyrosine kinases regulated with near-infrared 
            light.
  JOURNAL   Nat Commun. 2019 Mar 8;10(1):1129. doi: 10.1038/s41467-019-08988-3.
  PUBMED    30850602
REFERENCE   2  (bases 1 to 8943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-03-8"; volume: "10(1):1129. doi"; pages: " 
            10.1038/s41467-019-08988-3"
COMMENT     Sequence Label: pmCherry-Dr-TrkB
FEATURES             Location/Qualifiers
     source          1..8943
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             934..1635
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="SKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQ
                     TAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFED
                     GGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGE
                     IKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEG
                     RHSTGGMDELYK"
     primer_bind     complement(1073..1091)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1341..1360
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4375..4395
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     promoter        complement(4496..4514)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4497..4514)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4534..4551)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4540..4764
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4810..5238
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4897..4916)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5107..5128
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5247..5267)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5252..5581
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5432..5567
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5494..5513
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5648..6442
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5702..5721)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6312..6331
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6616..6737
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6653..6672)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6707..6726
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6786..6802)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6786..6802)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6799..6821)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6810..6826
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6834..6840,6841..6858,6859..6864))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6879..6900
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7017..7034)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7188..7776)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7268..7287)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7947..8738,8739..8807))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8570..8589
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8808..8912)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 atgggatgta ttaaatcaaa aaggaaagac gctagcaagg gcgaggagga taacatggcc
      961 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag
     1021 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
     1081 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg
     1141 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc
     1201 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
     1261 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc
     1321 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
     1381 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag
     1441 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc
     1501 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag
     1561 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg
     1621 gacgagctgt acaaggaatt cagtgctggt agtgctggta gtgctggcac cggtatgagc
     1681 cgggacccgt tgcccttttt tccaccgctt taccttggtg gcccggaaat taccaccgag
     1741 aactgcgagc gcgagccgat tcatattccc ggcagcatcc agccgcacgg cgccctgctc
     1801 actgccgacg ggcacagcgg cgaggtgctc cagatgagcc tcaacgcggc cacttttttg
     1861 ggacaggaac ccacagtgct gcgcggacag accctcgccg cactgctgcc cgagcagtgg
     1921 cccgcgctgc aagcggccct gccccccggc tgccccgacg ccctgcaata ccgcgcaacg
     1981 ctggactggc ctgccgccgg gcacctttcg ctgacggtgc accgggtcgg cgagttgctg
     2041 attctggagt tcgagccgac ggaggcctgg gacagcaccg ggccgcacgc gctgcgcaac
     2101 gcgatgttcg cgttcgaaag tgcccccaac ctgcgggcgc tggccgaggt ggcgacccag
     2161 acggtccgcg agctgacggg ctttgaccgg gtgatgctct acaaatttgc ccccgacgcc
     2221 accggcgaag tgattgccga ggcccgccgt gaggggctgc acgcctttct gggccaccgt
     2281 tttcccgcgt cggacattcc ggcgcaggcc cgcgcgctct acacccggca cctgctgcgc
     2341 ctgaccgccg acacccgcgc cgccaccgtg ccgctcgatc ccgtcctcaa cccgcagacg
     2401 aatgcgccca ccccgctggg cggcgccgtg ctgcgcgcca cctcgcccat gcacatgcag
     2461 tacctgcgga acatgggcgt cgggtcgagc ctgtcggtgt cggtggtggt cggcggccag
     2521 ctctggggcc tgatcgcctg ccaccaccag acgccctacg tgttgccgcc cgacctgcga
     2581 accacgctcg aatacctggg ccgcttgctg agcctgcaag ttcaggtcaa ggaagcggcg
     2641 gacgtggcgg cctttcgcca gagcctgcgg gagcaccacg cgcgggtggc cctcgcggcg
     2701 gcgcactcgc tctcgccgca cgacaccctc agtgacccgg cgcttgacct gctgggcctg
     2761 atgcgggccg ggggcctgat tctgcgtttc gagggccgct ggcagacgtt gggtgaagtg
     2821 ccgcctgccc cggcggtgga cgcgctgctg gcgtggctcg aaacccagcc gggcgccctg
     2881 gtccagaccg acgcgctagg ccaactgtgg ctcgccggcg ccgatctcgc ccccagcgca
     2941 gcgggcctgc tcgccatcag cgtgggcgag ggctggtcgg agtgcctcgt ctggctgcgg
     3001 cccgaactgc ggctggaggt cgcctggggc ggggccactc ctgaccaggc gaaagacgac
     3061 ctcgggccgc gccactcatt cgacacctac ctcgaagaaa aacgcggcta cgccgagccc
     3121 tggcatcccg gcgaaatcga ggaggcgcag gatctacgtg acacattgac cggggcgctg
     3181 ggcgaggcag aggcagcagc aaaggaggca gcagcaaagg aagcggcggc gaaagaagcg
     3241 gcggcgaaag cgaagttggc gagacattcc aagtttggca tgaaaggccc agcttccgtc
     3301 atcagcaacg acgatgactc tgccagccct ctccaccaca tctccaacgg gagcaacact
     3361 ccgtcttctt cggagggcgg gcccgatgct gtcatcattg ggatgaccaa gatccctgtc
     3421 attgaaaacc cccagtactt cggtatcacc aacagccagc tcaagccgga cacatttgtt
     3481 cagcacatca agagacacaa catcgttctg aagagggagc ttggagaagg agcctttggg
     3541 aaagttttcc tagcggagtg ctataacctc tgccccgagc aggataagat cctggtggcc
     3601 gtgaagacgc tgaaggacgc cagcgacaat gctcgcaagg actttcatcg cgaagccgag
     3661 ctgctgacca acctccagca cgagcacatt gtcaagttct acggtgtctg tgtggagggc
     3721 gacccactca tcatggtctt tgagtacatg aagcacgggg acctcaacaa gttccttagg
     3781 gcacacgggc cagatgcagt gctgatggca gagggtaacc cgcccaccga gctgacgcag
     3841 tcgcagatgc tgcacatcgc tcagcaaatc gcagcaggca tggtctacct ggcatcccaa
     3901 cacttcgtgc accgagacct ggccacccgg aactgcttgg taggagagaa cctgctggtg
     3961 aaaattgggg acttcgggat gtcccgggat gtatacagca ccgactacta ccgggttggt
     4021 ggccacacaa tgttgcccat ccgatggatg cctccagaga gcatcatgta caggaaattc
     4081 accaccgaga gtgacgtctg gagcctggga gttgtgttgt gggagatctt cacctacggc
     4141 aagcagccct ggtatcagct atcaaacaac gaggtgatag aatgcatcac ccagggcaga
     4201 gtccttcagc ggcctcgcac gtgtccccag gaggtgtacg agctgatgct gggatgctgg
     4261 cagcgggaac cacacacaag gaagaacatc aagaacatcc acacactcct tcagaacttg
     4321 gcgaaggcgt cgcccgtcta cctggacatc ctaggctagc tcgagccaac cactgaggat
     4381 ctgtactttc agagcgataa cgatggatcc actagtaacg gccgccagtg tgctggaatt
     4441 ctgcagatat ccatcacact ggcggccgct cgagcatgca tctagagggc cctattctat
     4501 agtgtcacct aaatgctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc
     4561 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     4621 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     4681 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     4741 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
     4801 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     4861 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     4921 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     4981 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     5041 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     5101 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     5161 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     5221 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     5281 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     5341 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     5401 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     5461 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     5521 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     5581 aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc
     5641 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     5701 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     5761 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     5821 tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     5881 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     5941 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     6001 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     6061 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     6121 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat
     6181 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     6241 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     6301 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     6361 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     6421 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg
     6481 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     6541 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     6601 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     6661 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     6721 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
     6781 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     6841 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     6901 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     6961 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     7021 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     7081 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     7141 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     7201 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     7261 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     7321 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     7381 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     7441 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     7501 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     7561 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     7621 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     7681 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     7741 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     7801 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     7861 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     7921 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     7981 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     8041 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     8101 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     8161 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     8221 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     8281 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     8341 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     8401 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     8461 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     8521 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     8581 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     8641 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     8701 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     8761 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     8821 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     8881 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     8941 gtc
//
