LOCUS       sequence-259282-        5571 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Myr DrTrkB expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5571)
  AUTHORS   Leopold AV, Chernov KG, Shemetov AA, Verkhusha VV
  TITLE     Neurotrophin receptor tyrosine kinases regulated with near-infrared 
            light.
  JOURNAL   Nat Commun. 2019 Mar 8;10(1):1129. doi: 10.1038/s41467-019-08988-3.
  PUBMED    30850602
REFERENCE   2  (bases 1 to 5571)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-03-8"; volume: "10(1):1129. doi"; pages: " 
            10.1038/s41467-019-08988-3"
COMMENT     Sequence Label: pCMVd2-Dr-TrkA
FEATURES             Location/Qualifiers
     source          1..5571
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(81..100)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    705..988
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
     promoter        1042..1093
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     1044..1064
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             3880..3900
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     primer_bind     complement(4031..4049)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    4086..4207
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4097..4116)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4151..4170
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             4606..5400
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MLEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4660..4679)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5270..5289
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      421 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
      481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatttcaag aagatccttt
      601 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      661 catgagatta tcaaaaagga tcttcaccta gatcctttta tagtccggaa atacaggaac
      721 gcacgctgga tggcccttcg ctgggatggt gaaaccatga aaaatggcag cttcagtgga
      781 ttaagtgggg gtaatgtggc ctgtaccctc tggttgcata ggtattcata cggttaaaat
      841 ttatcaggcg cgattgcggc agtttttcgg gtggtttgtt gccattttta cctgtctgct
      901 gccgtgatcg cgctgaacgc gttttagcgg tgcgtacaat taagggatta tggtaaatcc
      961 acttactgtc tgccctcgta gccatcgaga taaaccgcag tactccggcc acgatgcgtc
     1021 cggcgtagag gatcgagatc tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     1081 tataagcaga gctggtttag tgaaccgtca gatcactaga agcttggtac catgggatgt
     1141 attaaatcaa aaaggaaaga cgctctgtac aaggaattca gtgctggtag tgctggtagt
     1201 gctggcaccg gtatgagccg ggacccgttg cccttttttc caccgcttta ccttggtggc
     1261 ccggaaatta ccaccgagaa ctgcgagcgc gagccgattc atattcccgg cagcatccag
     1321 ccgcacggcg ccctgctcac tgccgacggg cacagcggcg aggtgctcca gatgagcctc
     1381 aacgcggcca cttttctggg acaggaaccc acagtgctgc gcggacagac cctcgccgca
     1441 ctgctgcccg agcagtggcc cgcgctgcaa gcggccctgc cccccggctg ccccgacgcc
     1501 ctgcaatacc gcgcaacgct ggactggcct gccgccgggc acctttcgct gacggtgcac
     1561 cgggtcggcg agttgctgat tctggagttc gagccgacgg aggcctggga cagcaccggg
     1621 ccgcacgcgc tgcgcaacgc gatgttcgcg ctcgaaagtg cccccaacct gcgggcgctg
     1681 gccgaggtgg cgacccagac ggtccgcgag ctgacgggct ttgaccgggt gatgctctac
     1741 aaatttgccc ccgacgccac cggcgaagtg attgccgagg cccgccgtga ggggctgcac
     1801 gcctttctgg gccaccgttt tcccgcgtcg gacattccgg cgcaggcccg cgcgctctac
     1861 acccggcacc tgctgcgcct gaccgccgac acccgcgccg ccgccgtgcc gctcgatccc
     1921 gtcctcaacc cgcagacgaa tgcgcccacc ccgctgggcg gcgccgtgct gcgcgccacc
     1981 tcgcccatgc acatgcagta cctgcggaac atgggcgtcg ggtcgagcct gtcggtgtcg
     2041 gtggtggtcg gcggccagct ctggggcctg atcgcctgcc accaccagac gccctacgtg
     2101 ttgccgcccg acctgcgaac cacgctcgaa tacctgggcc gcttgctgag cctgcaagtt
     2161 caggtcaagg aagcggcgga cgtggcggcc tttcgccaga gcctgcggga gcaccacgcg
     2221 cgggtggccc tcgcggcggc gcactcgctc tcgccgcacg acaccctcag tgacccggcg
     2281 cttgacctgc tgggcctgat gcgggccggg ggcctgattc tgcgtttcga gggccgctgg
     2341 cagacgttgg gtgaagtgcc gcctgccccg gcggtggacg cgctgctggc gtggctcgaa
     2401 acccagccgg gcgccctggt ccagaccgac gcgctaggcc aactgtggcc cgccggcgcc
     2461 gatctcgccc ccagcgcagc gggcctgctc gccatcagcg tgggcgaggg ctggtcggag
     2521 tgcctcgtct ggctgcggcc cgaactgcgg ctggaggtcg cctggggcgg ggccactcct
     2581 gaccaggcga aagacgacct cgggccgcgc cactcattcg acacctacct cgaagaaaaa
     2641 cgcggctacg ccgagccctg gcatcccggc gaaatcgagg aggcgcagga tctacgtgac
     2701 acattgaccg gggcgctggg cgaggcagag gcagcagcaa aggaggcagc agcaaaggaa
     2761 gcggcggcga aagaagcggc ggcgaaagcg aacaaatgtg gacagaggag caaatttggg
     2821 atcaaccgcc ctgctgtgct ggcgccagag gatgggctgg ccatgtccct acacttcatg
     2881 acactgggtg gcagttctct ttcccctact gagggcaaag gctccggact ccagggccac
     2941 atcatggaga acccacagta cttcagtgat acctgtgtcc accatatcaa gcgccaggac
     3001 atcattctca agtgggagct aggggaggga gcctttggaa aggtctttct tgctgagtgc
     3061 tacaaccttc tgaatgatca ggacaagatg ctggtggctg tcaaggcact gaaggagaca
     3121 tctgagaatg ctcgtcagga cttccatcgt gaggcagagc tgctcaccat gctacagcac
     3181 caacacatcg tacgcttctt tggagtctgc acggagggtg gcccattgct catggtcttc
     3241 gagtacatgc gccatgggga cctcaaccgt ttcctccggt cccacggacc tgatgcaaaa
     3301 ctgctggctg gcggcgagga tgtggctcct ggtcctttgg gccttgggca gcttctggct
     3361 gtggctagcc aggtggctgc tggtatggtg tacctagcca gcctgcactt tgtgcaccgg
     3421 gacctggcca cacgcaactg tctggtgggt cagggactag tggtcaagat tggagacttc
     3481 ggcatgagca gggacatcta cagcacagac tactaccgtg tgggaggtcg gaccatgctg
     3541 cccatccgct ggatgcctcc agagagcatc ctctaccgca agttcagcac cgagagtgat
     3601 gtgtggagct tcggggtggt gctctgggag atcttcacct atggaaagca accctggtac
     3661 cagctctcca acactgaggc gatcgagtgc atcacgcagg gccgggagct ggagcggccg
     3721 cgcgcctgcc ctcctgatgt ctacgccatc atgcgcggct gctggcagcg tgagccgcaa
     3781 cagcgcctca gcatgaagga tgtgcacgcg cggctgcagg ccttggcaca ggcgccaccg
     3841 agttacctgg acgttctggg ctagctcgag ccaaccactg aggatctgta ctttcagagc
     3901 gataacgatg gatccagtct ccccgggagc tcatatgtgt cacttaagta tatctagagt
     3961 cgacctgcag gcatgcaagc tgatccggct gctaacaaag cccgaaagga agctgagttg
     4021 gctgctgcca ccgctgagca ataactagca taaccccttg gggcggccgc ttcgagcaga
     4081 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     4141 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     4201 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg agatgtggga
     4261 ggttttttta agcaagtaaa acctctacaa atgtggtaaa atcgtattct aatggagcct
     4321 ctttgcgctt gcgttttccc ttgtccagat agcccagtag ctgacattca tccggggtca
     4381 gcaccgtttc tgcggactgg ctttctacgt gttccgcttc ctttagcagc ccttgcgccc
     4441 tgagtgcttg cggcagcgtg agcttcaaaa gaattgccag ctggggcgcc ctctggtaag
     4501 gttgggaagc cctgcaaagt aaactggatg gctttcttgc cgccaaggat ctgatggcgc
     4561 aggggatcaa gatctgatca agagacagga tgacggtcgt ttcgcatgct tgaacaagat
     4621 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     4681 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     4741 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
     4801 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     4861 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     4921 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     4981 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgc
     5041 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     5101 gcgccagccg aactgttcgc caggctcaag gcgcgtatgc cggatggtga ggatctcgtc
     5161 gtgactcatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     5221 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     5281 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     5341 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     5401 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caaccggtat cagctcactc
     5461 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     5521 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt t
//
