LOCUS       sequence-259289-       11000 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expressing GAG precursor protein, in which COM is inserted after the
            second zinc finger domain of nucleocapsid protein (NC).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11000)
  AUTHORS   Lyu P, Javidi-Parsijani P, Atala A, Lu B
  TITLE     Delivering Cas9/sgRNA ribonucleoprotein (RNP) by lentiviral 
            capsid-based bionanoparticles for efficient 'hit-and-run' genome 
            editing.
  JOURNAL   Nucleic Acids Res. 2019 Jul 12. pii: 5531787. doi: 
            10.1093/nar/gkz605.
  PUBMED    31299082
REFERENCE   2  (bases 1 to 11000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2019 Jul 12. pii: 5531787. doi: 10.1093/nar/gkz605."
COMMENT     Sequence Label: psPAX2-D64V-NC-COM
FEATURES             Location/Qualifiers
     source          1..11000
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        5..384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          662..1678
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1602..1624
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1686..1705
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             3370..6381
                     /codon_start=1
                     /gene="pol"
                     /product="pol protein from human immunodeficiency virus 1"
                     /label=HIV-1 pol
                     /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
                     ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
                     GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
                     DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
                     NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
                     AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLVCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     MAGDDCVASRQDED"
     misc_feature    6066..6183
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    6955..7188
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7373..7417
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             7711..7737
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     primer_bind     complement(8022..8041)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    8087..8142
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8141..8160)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(8503..8519)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8503..8519)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8516..8538)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8527..8543
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8551..8557,8558..8575,8576..8581))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8596..8617
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8676..8871
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8722..8857
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    8877..9011
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8914..8933)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8968..8987
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9079..9096)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9250..9838)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9330..9349)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10009..10800,10801..10869))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10632..10651
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10870..10974)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
       61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      361 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggsgcgc gccrggsggg gsggggsggg gsgrggggsg
      541 gggsggggsg rggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
      721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      781 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
      841 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
      901 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
      961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
     1021 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     1081 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
     1141 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
     1201 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     1261 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
     1321 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
     1381 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
     1441 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
     1501 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     1561 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     1621 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     1681 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tcgggccgcg
     1741 ttgacgcgca cggcaagagg cgaggggcgg cgactggtga gagatgggtg cgagagcgtc
     1801 agtattaagc gggggagaat tagatcgatg ggaaaaaatt cggttaaggc cagggggaaa
     1861 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt
     1921 taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc
     1981 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta
     2041 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga
     2101 agagcaaaac aaaagtaaga aaaaagcaca gcaagcagca gctgacacag gacacagcaa
     2161 tcaggtcagc caaaattacc ctatagtgca gaacatccag gggcaaatgg tacatcaggc
     2221 catatcacct agaactttaa atgcatgggt aaaagtagta gaagagaagg ctttcagccc
     2281 agaagtgata cccatgtttt cagcattatc agaaggagcc accccacaag atttaaacac
     2341 catgctaaac acagtggggg gacatcaagc agccatgcaa atgttaaaag agaccatcaa
     2401 tgaggaagct gcagaatggg atagagtgca tccagtgcat gcagggccta ttgcaccagg
     2461 ccagatgaga gaaccaaggg gaagtgacat agcaggaact actagtaccc ttcaggaaca
     2521 aataggatgg atgacacata atccacctat cccagtagga gaaatctata aaagatggat
     2581 aatcctggga ttaaataaaa tagtaagaat gtatagccct accagcattc tggacataag
     2641 acaaggacca aaggaaccct ttagagacta tgtagaccga ttctataaaa ctctaagagc
     2701 cgagcaagct tcacaagagg taaaaaattg gatgacagaa accttgttgg tccaaaatgc
     2761 gaacccagat tgtaagacta ttttaaaagc attgggacca ggagcgacac tagaagaaat
     2821 gatgacagca tgtcagggag tggggggacc cggccataaa gcaagagttt tggctgaagc
     2881 aatgagccaa gtaacaaatc cagctaccat aatgatacag aaaggcaatt ttaggaacca
     2941 aagaaagact gttaagtgtt tcaattgtgg caaagaaggg cacatagcca aaaattgcag
     3001 ggcccctagg aaaaagggct gttggaaatg tggaaaggaa ggacaccaaa tgaaagattg
     3061 ttccggtgga ggtggatcca tggcttctaa ctttactcag ttcgttctcg tcgacaatgg
     3121 cggaactggc gacgtgatga aatcaattcg ctgtaaaaac tgcaacaaac tgttatttaa
     3181 ggcggattcc tttgatcaca ttgaaatcag gtgtccgcgt tgcaaacgtc acatcataat
     3241 gctgaatgcc tgcgagcatc ccacggagaa acattgtggg aaaagagaaa aaatcacgca
     3301 ttctgacgaa accgtgcgtt atggcggtca caactccgga ggtggaggtg gaactgagag
     3361 acaggctaat tttttaggga agatctggcc ttcccacaag ggaaggccag ggaattttct
     3421 tcagagcaga ccagagccaa cagccccacc agaagagagc ttcaggtttg gggaagagac
     3481 aacaactccc tctcagaagc aggagccgat agacaaggaa ctgtatcctt tagcttccct
     3541 cagatcactc tttggcagcg acccctcgtc acaataaaga taggggggca attaaaggaa
     3601 gctctattag atacaggagc agatgataca gtattagaag aaatgaattt gccaggaaga
     3661 tggaaaccaa aaatgatagg gggaattgga ggttttatca aagtaagaca gtatgatcag
     3721 atactcatag aaatctgcgg acataaagct ataggtacag tattagtagg acctacacct
     3781 gtcaacataa ttggaagaaa tctgttgact cagattggct gcactttaaa ttttcccatt
     3841 agtcctattg agactgtacc agtaaaatta aagccaggaa tggatggccc aaaagttaaa
     3901 caatggccat tgacagaaga aaaaataaaa gcattagtag aaatttgtac agaaatggaa
     3961 aaggaaggaa aaatttcaaa aattgggcct gaaaatccat acaatactcc agtatttgcc
     4021 ataaagaaaa aagacagtac taaatggaga aaattagtag atttcagaga acttaataag
     4081 agaactcaag atttctggga agttcaatta ggaataccac atcctgcagg gttaaaacag
     4141 aaaaaatcag taacagtact ggatgtgggc gatgcatatt tttcagttcc cttagataaa
     4201 gacttcagga agtatactgc atttaccata cctagtataa acaatgagac accagggatt
     4261 agatatcagt acaatgtgct tccacaggga tggaaaggat caccagcaat attccagtgt
     4321 agcatgacaa aaatcttaga gccttttaga aaacaaaatc cagacatagt catctatcaa
     4381 tacatggatg atttgtatgt aggatctgac ttagaaatag ggcagcatag aacaaaaata
     4441 gaggaactga gacaacatct gttgaggtgg ggatttacca caccagacaa aaaacatcag
     4501 aaagaacctc cattcctttg gatgggttat gaactccatc ctgataaatg gacagtacag
     4561 cctatagtgc tgccagaaaa ggacagctgg actgtcaatg acatacagaa attagtggga
     4621 aaattgaatt gggcaagtca gatttatgca gggattaaag taaggcaatt atgtaaactt
     4681 cttaggggaa ccaaagcact aacagaagta gtaccactaa cagaagaagc agagctagaa
     4741 ctggcagaaa acagggagat tctaaaagaa ccggtacatg gagtgtatta tgacccatca
     4801 aaagacttaa tagcagaaat acagaagcag gggcaaggcc aatggacata tcaaatttat
     4861 caagagccat ttaaaaatct gaaaacagga aagtatgcaa gaatgaaggg tgcccacact
     4921 aatgatgtga aacaattaac agaggcagta caaaaaatag ccacagaaag catagtaata
     4981 tggggaaaga ctcctaaatt taaattaccc atacaaaagg aaacatggga agcatggtgg
     5041 acagagtatt ggcaagccac ctggattcct gagtgggagt ttgtcaatac ccctccctta
     5101 gtgaagttat ggtaccagtt agagaaagaa cccataatag gagcagaaac tttctatgta
     5161 gatggggcag ccaataggga aactaaatta ggaaaagcag gatatgtaac tgacagagga
     5221 agacaaaaag ttgtccccct aacggacaca acaaatcaga agactgagtt acaagcaatt
     5281 catctagctt tgcaggattc gggattagaa gtaaacatag tgacagactc acaatatgca
     5341 ttgggaatca ttcaagcaca accagataag agtgaatcag agttagtcag tcaaataata
     5401 gagcagttaa taaaaaagga aaaagtctac ctggcatggg taccagcaca caaaggaatt
     5461 ggaggaaatg aacaagtaga taaattggtc agtgctggaa tcaggaaagt actattttta
     5521 gatggaatag ataaggccca agaagaacat gagaaatatc acagtaattg gagagcaatg
     5581 gctagtgatt ttaacctacc acctgtagta gcaaaagaaa tagtagccag ctgtgataaa
     5641 tgtcagctaa aaggggaagc catgcatgga caagtagact gtagcccagg aatatggcag
     5701 ctagtttgta cacatttaga aggaaaagtt atcttggtag cagttcatgt agccagtgga
     5761 tatatagaag cagaagtaat tccagcagag acagggcaag aaacagcata cttcctctta
     5821 aaattagcag gaagatggcc agtaaaaaca gtacatacag acaatggcag caatttcacc
     5881 agtactacag ttaaggccgc ctgttggtgg gcggggatca agcaggaatt tggcattccc
     5941 tacaatcccc aaagtcaagg agtaatagaa tctatgaata aagaattaaa gaaaattata
     6001 ggacaggtaa gagatcaggc tgaacatctt aagacagcag tacaaatggc agtattcatc
     6061 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
     6121 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
     6181 tttcgggttt attacaggga cagcagagat ccagtttgga aaggaccagc aaagctcctc
     6241 tggaaaggtg aaggggcagt agtaatacaa gataatagtg acataaaagt agtgccaaga
     6301 agaaaagcaa agatcatcag ggattatgga aaacagatgg caggtgatga ttgtgtggca
     6361 agtagacagg atgaggatta acacatggaa ttctgcaaca actgctgttt atccatttca
     6421 gaattgggtg tcgacatagc agaataggcg ttactcgaca gaggagagca agaaatggag
     6481 ccagtagatc ctagactaga gccctggaag catccaggaa gtcagcctaa aactgcttgt
     6541 accaattgct attgtaaaaa gtgttgcttt cattgccaag tttgtttcat gacaaaagcc
     6601 ttaggcatct cctatggcag gaagaagcgg agacagcgac gaagagctca tcagaacagt
     6661 cagactcatc aagcttctct atcaaagcag taagtagtac atgtaatgca acctataata
     6721 gtagcaatag tagcattagt agtagcaata ataatagcaa tagttgtgtg gtccatagta
     6781 atcatagaat ataggaaaat ggccgctgat cttcagacct ggaggaggag atatgaggga
     6841 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     6901 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     6961 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     7021 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     7081 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     7141 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     7201 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     7261 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     7321 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     7381 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     7441 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     7501 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     7561 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     7621 tggagagaga gacagagaca gatccattcg attagtgaac ggatccttgg cacttatctg
     7681 ggacgatctg cggagcctgt gcctcttcag ctaccaccgc ttgagagact tactcttgat
     7741 tgtaacgagg attgtggaac ttctgggacg cagggggtgg gaagccctca aatattggtg
     7801 gaatctccta caatattgga gtcaggagct aaagaatagt gctgttagct tgctcaatgc
     7861 cacagccata gcagtagctg aggggacaga tagggttata gaagtagtac aaggagcttg
     7921 tagagctatt cgccacatac ctagaagaat aagacagggc ttggaaagga ttttgctata
     7981 agctcgaaac aaccggtacc tctagaacta tagctagcag atctttttcc ctctgccaaa
     8041 aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt
     8101 attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg
     8161 agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc
     8221 catatgctgg ctgccatgaa caaaggttgg ctataaagag gtcatcagta tatgaaacag
     8281 ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta gatttttttt
     8341 atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttta
     8401 ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc tcttctctta
     8461 tggagatccc tcgacctgca gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt
     8521 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     8581 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     8641 tttccagtcg ggaaacctgt cgtgccagcg gatccgcatc tcaattagtc agcaaccata
     8701 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     8761 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     8821 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctaact
     8881 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     8941 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     9001 atgtctggat ccgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     9061 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     9121 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     9181 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     9241 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     9301 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     9361 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     9421 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     9481 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     9541 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     9601 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     9661 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     9721 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     9781 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     9841 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     9901 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     9961 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
    10021 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
    10081 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
    10141 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
    10201 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
    10261 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    10321 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    10381 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    10441 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    10501 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    10561 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    10621 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    10681 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    10741 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    10801 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    10861 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    10921 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    10981 tccccgaaaa gtgccacctg
//
