LOCUS       sequence-259305-         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The plasmid includes a miRFP703 gene driven by a minimal CMV 
            promoter. Cis-regulatory elements can be cloned into the single 
            EcoRV site. This plasmid can be used for generating a lentiviral 
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Roman Azcona MS, Fang Y, Carusillo A, Cathomen T, Mussolino C
  TITLE     A versatile reporter system for multiplexed screening of effective 
            epigenome editors.
  JOURNAL   Nat Protoc. 2020 Oct;15(10):3410-3440. doi: 
            10.1038/s41596-020-0380-y. Epub 2020 Sep 4.
  PUBMED    32887975
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41596-020-0380-y"; journalName: "Nat Protoc"; date: 
            "2020-10"; volume: "15"; issue: "10"; pages: "3410-3440"
COMMENT     Sequence Label: pCCl-MCS_miniCMV_miRFP703
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggttgctg ggcacgcctc tggatcaccc gcgttcggca ctgcaagtca ttctaactgc
       61 gagcacgagg agatacacct ggcagggagt atacaacctc atggggcact tctcgttgtg
      121 tccgaacatg accacagggt gatacaggcg tctgctaatg ccgctgagtt cctgaatctt
      181 ggtagtgtat tgggagtgcc gctggcggag atagacggtg atctgttgat taagatcctc
      241 ccccacctcg atcctactgc ggaggggatg cctgttgctg ttagatgccg catcggtaat
      301 cctagcacgg agtattgcgg gcttatgcat cgaccccccg aaggggggtt gatcatcgaa
      361 ctagagcggg ctgggcccag tatcgatttg agtggcacac tggcgcccgc tcttgaaagg
      421 atacgaacag cggggtcctt gcgagcgttg tgtgatgata ccgttctgtt gtttcaacaa
      481 tgcactggct acgatcgcgt aatggtttat aggtttgacg agcaaggaca cgggcttgtc
      541 tttagtgagt gccatgtgcc cggcttggaa tcctatttcg gcaataggta tccgagtagt
      601 ctcgttcctc agatggctcg gcaactgtac gtgagacaaa gggtgcgggt cttggtagat
      661 gttacttacc aacctgtccc cctggagcca cgcctgtctc cgctgacggg gcgagacctg
      721 gatatgagcg ggtgtttcct gagaagtatg agtcccatcc acctgcaatt cctcaaagat
      781 atgggagtac gcgctacgtt ggcagtgtcc cttgttgttg ggggtaagct ctggggcctg
      841 gtggtgtgcc atcattacct tccccgcttc attaggtttg agctgagggc tatttgcaaa
      901 cggttggcgg aacgcattgc aactaggata acagcactcg aatcatga
//
