LOCUS       sequence-259306-         702 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The plasmid includes a TagBFP gene driven by a minimal CMV promoter.
            Cis-regulatory elements can be cloned into the single EcoRV site. 
            This plasmid can be used for generating a lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Roman Azcona MS, Fang Y, Carusillo A, Cathomen T, Mussolino C
  TITLE     A versatile reporter system for multiplexed screening of effective 
            epigenome editors.
  JOURNAL   Nat Protoc. 2020 Oct;15(10):3410-3440. doi: 
            10.1038/s41596-020-0380-y. Epub 2020 Sep 4.
  PUBMED    32887975
REFERENCE   2  (bases 1 to 702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41596-020-0380-y"; journalName: "Nat Protoc"; date: 
            "2020-10"; volume: "15"; issue: "10"; pages: "3410-3440"
COMMENT     Sequence Label: pCCl-MCS_miniCMV_TagBFP
FEATURES             Location/Qualifiers
     source          1..702
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..702
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
ORIGIN
        1 atgagcgagc tgattaagga gaacatgcac atgaagctgt acatggaggg caccgtggac
       61 aaccatcact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc
      121 atgagaatca aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctact
      181 agcttcctct acggcagcaa gaccttcatc aaccacaccc agggcatccc cgacttcttc
      241 aagcagtcct tccctgaggg cttcacatgg gagagagtca ccacatacga agacgggggc
      301 gtgctgaccg ctacccagga caccagcctc caggacggct gcctcatcta caacgtcaag
      361 atcagagggg tgaacttcac atccaacggc cctgtgatgc agaagaaaac actcggctgg
      421 gaggccttca ccgagacgct gtaccccgct gacggcggcc tggaaggcag aaacgacatg
      481 gccctgaagc tcgtgggcgg gagccatctg atcgcaaaca tcaagaccac atatagatcc
      541 aagaaacccg ctaagaacct caagatgcct ggcgtctact atgtggacta cagactggaa
      601 agaatcaagg aggccaacaa cgagacctac gtcgagcagc acgaggtggc agtggccaga
      661 tactgcgacc tccctagcaa actggggcac aagcttaatt aa
//
