LOCUS       sequence-259310-        6297 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Myr mCherryDrTrkB expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6297)
  AUTHORS   Leopold AV, Chernov KG, Shemetov AA, Verkhusha VV
  TITLE     Neurotrophin receptor tyrosine kinases regulated with near-infrared 
            light.
  JOURNAL   Nat Commun. 2019 Mar 8;10(1):1129. doi: 10.1038/s41467-019-08988-3.
  PUBMED    30850602
REFERENCE   2  (bases 1 to 6297)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-03-8"; volume: "10(1):1129. doi"; pages: " 
            10.1038/s41467-019-08988-3"
COMMENT     Sequence Label: pCMVd2-mCherry-Dr-TrkB
FEATURES             Location/Qualifiers
     source          1..6297
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(81..100)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    705..988
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
     promoter        1042..1093
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     1044..1064
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             1165..1866
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="SKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQ
                     TAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFED
                     GGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGE
                     IKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEG
                     RHSTGGMDELYK"
     primer_bind     complement(1304..1322)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1572..1591
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4606..4626
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     primer_bind     complement(4757..4775)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    4812..4933
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4823..4842)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4877..4896
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             5332..6126
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MLEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5386..5405)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5996..6015
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      421 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
      481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatttcaag aagatccttt
      601 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      661 catgagatta tcaaaaagga tcttcaccta gatcctttta tagtccggaa atacaggaac
      721 gcacgctgga tggcccttcg ctgggatggt gaaaccatga aaaatggcag cttcagtgga
      781 ttaagtgggg gtaatgtggc ctgtaccctc tggttgcata ggtattcata cggttaaaat
      841 ttatcaggcg cgattgcggc agtttttcgg gtggtttgtt gccattttta cctgtctgct
      901 gccgtgatcg cgctgaacgc gttttagcgg tgcgtacaat taagggatta tggtaaatcc
      961 acttactgtc tgccctcgta gccatcgaga taaaccgcag tactccggcc acgatgcgtc
     1021 cggcgtagag gatcgagatc tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     1081 tataagcaga gctggtttag tgaaccgtca gatcactaga agcttggtac catgggatgt
     1141 attaaatcaa aaaggaaaga cgctagcaag ggcgaggagg ataacatggc catcatcaag
     1201 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
     1261 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
     1321 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
     1381 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
     1441 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
     1501 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
     1561 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg
     1621 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
     1681 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
     1741 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
     1801 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg
     1861 tacaaggaat tcagtgctgg tagtgctggt agtgctggca ccggtatgag ccgggacccg
     1921 ttgccctttt ttccaccgct ttaccttggt ggcccggaaa ttaccaccga gaactgcgag
     1981 cgcgagccga ttcatattcc cggcagcatc cagccgcacg gcgccctgct cactgccgac
     2041 gggcacagcg gcgaggtgct ccagatgagc ctcaacgcgg ccactttttt gggacaggaa
     2101 cccacagtgc tgcgcggaca gaccctcgcc gcactgctgc ccgagcagtg gcccgcgctg
     2161 caagcggccc tgccccccgg ctgccccgac gccctgcaat accgcgcaac gctggactgg
     2221 cctgccgccg ggcacctttc gctgacggtg caccgggtcg gcgagttgct gattctggag
     2281 ttcgagccga cggaggcctg ggacagcacc gggccgcacg cgctgcgcaa cgcgatgttc
     2341 gcgttcgaaa gtgcccccaa cctgcgggcg ctggccgagg tggcgaccca gacggtccgc
     2401 gagctgacgg gctttgaccg ggtgatgctc tacaaatttg cccccgacgc caccggcgaa
     2461 gtgattgccg aggcccgccg tgaggggctg cacgcctttc tgggccaccg ttttcccgcg
     2521 tcggacattc cggcgcaggc ccgcgcgctc tacacccggc acctgctgcg cctgaccgcc
     2581 gacacccgcg ccgccaccgt gccgctcgat cccgtcctca acccgcagac gaatgcgccc
     2641 accccgctgg gcggcgccgt gctgcgcgcc acctcgccca tgcacatgca gtacctgcgg
     2701 aacatgggcg tcgggtcgag cctgtcggtg tcggtggtgg tcggcggcca gctctggggc
     2761 ctgatcgcct gccaccacca gacgccctac gtgttgccgc ccgacctgcg aaccacgctc
     2821 gaatacctgg gccgcttgct gagcctgcaa gttcaggtca aggaagcggc ggacgtggcg
     2881 gcctttcgcc agagcctgcg ggagcaccac gcgcgggtgg ccctcgcggc ggcgcactcg
     2941 ctctcgccgc acgacaccct cagtgacccg gcgcttgacc tgctgggcct gatgcgggcc
     3001 gggggcctga ttctgcgttt cgagggccgc tggcagacgt tgggtgaagt gccgcctgcc
     3061 ccggcggtgg acgcgctgct ggcgtggctc gaaacccagc cgggcgccct ggtccagacc
     3121 gacgcgctag gccaactgtg gctcgccggc gccgatctcg cccccagcgc agcgggcctg
     3181 ctcgccatca gcgtgggcga gggctggtcg gagtgcctcg tctggctgcg gcccgaactg
     3241 cggctggagg tcgcctgggg cggggccact cctgaccagg cgaaagacga cctcgggccg
     3301 cgccactcat tcgacaccta cctcgaagaa aaacgcggct acgccgagcc ctggcatccc
     3361 ggcgaaatcg aggaggcgca ggatctacgt gacacattga ccggggcgct gggcgaggca
     3421 gaggcagcag caaaggaggc agcagcaaag gaagcggcgg cgaaagaagc ggcggcgaaa
     3481 gcgaagttgg cgagacattc caagtttggc atgaaaggcc cagcttccgt catcagcaac
     3541 gacgatgact ctgccagccc tctccaccac atctccaacg ggagcaacac tccgtcttct
     3601 tcggagggcg ggcccgatgc tgtcatcatt gggatgacca agatccctgt cattgaaaac
     3661 ccccagtact tcggtatcac caacagccag ctcaagccgg acacatttgt tcagcacatc
     3721 aagagacaca acatcgttct gaagagggag cttggagaag gagcctttgg gaaagttttc
     3781 ctagcggagt gctataacct ctgccccgag caggataaga tcctggtggc cgtgaagacg
     3841 ctgaaggacg ccagcgacaa tgctcgcaag gactttcatc gcgaagccga gctgctgacc
     3901 aacctccagc acgagcacat tgtcaagttc tacggtgtct gtgtggaggg cgacccactc
     3961 atcatggtct ttgagtacat gaagcacggg gacctcaaca agttccttag ggcacacggg
     4021 ccagatgcag tgctgatggc agagggtaac ccgcccaccg agctgacgca gtcgcagatg
     4081 ctgcacatcg ctcagcaaat cgcagcaggc atggtctacc tggcatccca acacttcgtg
     4141 caccgagacc tggccacccg gaactgcttg gtaggagaga acctgctggt gaaaattggg
     4201 gacttcggga tgtcccggga tgtatacagc accgactact accgggttgg tggccacaca
     4261 atgttgccca tccgatggat gcctccagag agcatcatgt acaggaaatt caccaccgag
     4321 agtgacgtct ggagcctggg agttgtgttg tgggagatct tcacctacgg caagcagccc
     4381 tggtatcagc tatcaaacaa cgaggtgata gaatgcatca cccagggcag agtccttcag
     4441 cggcctcgca cgtgtcccca ggaggtgtac gagctgatgc tgggatgctg gcagcgggaa
     4501 ccacacacaa ggaagaacat caagaacatc cacacactcc ttcagaactt ggcgaaggcg
     4561 tcgcccgtct acctggacat cctaggctag ctcgagccaa ccactgagga tctgtacttt
     4621 cagagcgata acgatggatc cagtctcccc gggagctcat atgtgtcact taagtatatc
     4681 tagagtcgac ctgcaggcat gcaagctgat ccggctgcta acaaagcccg aaaggaagct
     4741 gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ggccgcttcg
     4801 agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
     4861 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     4921 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggagat
     4981 gtgggaggtt tttttaagca agtaaaacct ctacaaatgt ggtaaaatcg tattctaatg
     5041 gagcctcttt gcgcttgcgt tttcccttgt ccagatagcc cagtagctga cattcatccg
     5101 gggtcagcac cgtttctgcg gactggcttt ctacgtgttc cgcttccttt agcagccctt
     5161 gcgccctgag tgcttgcggc agcgtgagct tcaaaagaat tgccagctgg ggcgccctct
     5221 ggtaaggttg ggaagccctg caaagtaaac tggatggctt tcttgccgcc aaggatctga
     5281 tggcgcaggg gatcaagatc tgatcaagag acaggatgac ggtcgtttcg catgcttgaa
     5341 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     5401 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     5461 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag
     5521 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     5581 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     5641 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     5701 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     5761 gcacgcactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     5821 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gtatgccgga tggtgaggat
     5881 ctcgtcgtga ctcatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     5941 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     6001 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     6061 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     6121 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac cggtatcagc
     6181 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     6241 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgtt
//
