LOCUS       sequence-259311-        6264 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Myr mCherryDrTrkA expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6264)
  AUTHORS   Leopold AV, Chernov KG, Shemetov AA, Verkhusha VV
  TITLE     Neurotrophin receptor tyrosine kinases regulated with near-infrared 
            light.
  JOURNAL   Nat Commun. 2019 Mar 8;10(1):1129. doi: 10.1038/s41467-019-08988-3.
  PUBMED    30850602
REFERENCE   2  (bases 1 to 6264)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-03-8"; volume: "10(1):1129. doi"; pages: " 
            10.1038/s41467-019-08988-3"
COMMENT     Sequence Label: pCMVd2-mCherry-Dr-TrkA
FEATURES             Location/Qualifiers
     source          1..6264
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(81..100)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    705..988
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
     promoter        1042..1093
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     1044..1064
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             1165..1866
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="SKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQ
                     TAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFED
                     GGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGE
                     IKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEG
                     RHSTGGMDELYK"
     primer_bind     complement(1304..1322)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1572..1591
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4573..4593
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     primer_bind     complement(4724..4742)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    4779..4900
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4790..4809)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4844..4863
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             5299..6093
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MLEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5353..5372)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5963..5982
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      421 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
      481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatttcaag aagatccttt
      601 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      661 catgagatta tcaaaaagga tcttcaccta gatcctttta tagtccggaa atacaggaac
      721 gcacgctgga tggcccttcg ctgggatggt gaaaccatga aaaatggcag cttcagtgga
      781 ttaagtgggg gtaatgtggc ctgtaccctc tggttgcata ggtattcata cggttaaaat
      841 ttatcaggcg cgattgcggc agtttttcgg gtggtttgtt gccattttta cctgtctgct
      901 gccgtgatcg cgctgaacgc gttttagcgg tgcgtacaat taagggatta tggtaaatcc
      961 acttactgtc tgccctcgta gccatcgaga taaaccgcag tactccggcc acgatgcgtc
     1021 cggcgtagag gatcgagatc tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     1081 tataagcaga gctggtttag tgaaccgtca gatcactaga agcttggtac catgggatgt
     1141 attaaatcaa aaaggaaaga cgctagcaag ggcgaggagg ataacatggc catcatcaag
     1201 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
     1261 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
     1321 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
     1381 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
     1441 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
     1501 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
     1561 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg
     1621 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
     1681 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
     1741 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
     1801 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg
     1861 tacaaggaat tcagtgctgg tagtgctggt agtgctggca ccggtatgag ccgggacccg
     1921 ttgccctttt ttccaccgct ttaccttggt ggcccggaaa ttaccaccga gaactgcgag
     1981 cgcgagccga ttcatattcc cggcagcatc cagccgcacg gcgccctgct cactgccgac
     2041 gggcacagcg gcgaggtgct ccagatgagc ctcaacgcgg ccacttttct gggacaggaa
     2101 cccacagtgc tgcgcggaca gaccctcgcc gcactgctgc ccgagcagtg gcccgcgctg
     2161 caagcggccc tgccccccgg ctgccccgac gccctgcaat accgcgcaac gctggactgg
     2221 cctgccgccg ggcacctttc gctgacggtg caccgggtcg gcgagttgct gattctggag
     2281 ttcgagccga cggaggcctg ggacagcacc gggccgcacg cgctgcgcaa cgcgatgttc
     2341 gcgctcgaaa gtgcccccaa cctgcgggcg ctggccgagg tggcgaccca gacggtccgc
     2401 gagctgacgg gctttgaccg ggtgatgctc tacaaatttg cccccgacgc caccggcgaa
     2461 gtgattgccg aggcccgccg tgaggggctg cacgcctttc tgggccaccg ttttcccgcg
     2521 tcggacattc cggcgcaggc ccgcgcgctc tacacccggc acctgctgcg cctgaccgcc
     2581 gacacccgcg ccgccgccgt gccgctcgat cccgtcctca acccgcagac gaatgcgccc
     2641 accccgctgg gcggcgccgt gctgcgcgcc acctcgccca tgcacatgca gtacctgcgg
     2701 aacatgggcg tcgggtcgag cctgtcggtg tcggtggtgg tcggcggcca gctctggggc
     2761 ctgatcgcct gccaccacca gacgccctac gtgttgccgc ccgacctgcg aaccacgctc
     2821 gaatacctgg gccgcttgct gagcctgcaa gttcaggtca aggaagcggc ggacgtggcg
     2881 gcctttcgcc agagcctgcg ggagcaccac gcgcgggtgg ccctcgcggc ggcgcactcg
     2941 ctctcgccgc acgacaccct cagtgacccg gcgcttgacc tgctgggcct gatgcgggcc
     3001 gggggcctga ttctgcgttt cgagggccgc tggcagacgt tgggtgaagt gccgcctgcc
     3061 ccggcggtgg acgcgctgct ggcgtggctc gaaacccagc cgggcgccct ggtccagacc
     3121 gacgcgctag gccaactgtg gcccgccggc gccgatctcg cccccagcgc agcgggcctg
     3181 ctcgccatca gcgtgggcga gggctggtcg gagtgcctcg tctggctgcg gcccgaactg
     3241 cggctggagg tcgcctgggg cggggccact cctgaccagg cgaaagacga cctcgggccg
     3301 cgccactcat tcgacaccta cctcgaagaa aaacgcggct acgccgagcc ctggcatccc
     3361 ggcgaaatcg aggaggcgca ggatctacgt gacacattga ccggggcgct gggcgaggca
     3421 gaggcagcag caaaggaggc agcagcaaag gaagcggcgg cgaaagaagc ggcggcgaaa
     3481 gcgaacaaat gtggacagag gagcaaattt gggatcaacc gccctgctgt gctggcgcca
     3541 gaggatgggc tggccatgtc cctacacttc atgacactgg gtggcagttc tctttcccct
     3601 actgagggca aaggctccgg actccagggc cacatcatgg agaacccaca gtacttcagt
     3661 gatacctgtg tccaccatat caagcgccag gacatcattc tcaagtggga gctaggggag
     3721 ggagcctttg gaaaggtctt tcttgctgag tgctacaacc ttctgaatga tcaggacaag
     3781 atgctggtgg ctgtcaaggc actgaaggag acatctgaga atgctcgtca ggacttccat
     3841 cgtgaggcag agctgctcac catgctacag caccaacaca tcgtacgctt ctttggagtc
     3901 tgcacggagg gtggcccatt gctcatggtc ttcgagtaca tgcgccatgg ggacctcaac
     3961 cgtttcctcc ggtcccacgg acctgatgca aaactgctgg ctggcggcga ggatgtggct
     4021 cctggtcctt tgggccttgg gcagcttctg gctgtggcta gccaggtggc tgctggtatg
     4081 gtgtacctag ccagcctgca ctttgtgcac cgggacctgg ccacacgcaa ctgtctggtg
     4141 ggtcagggac tagtggtcaa gattggagac ttcggcatga gcagggacat ctacagcaca
     4201 gactactacc gtgtgggagg tcggaccatg ctgcccatcc gctggatgcc tccagagagc
     4261 atcctctacc gcaagttcag caccgagagt gatgtgtgga gcttcggggt ggtgctctgg
     4321 gagatcttca cctatggaaa gcaaccctgg taccagctct ccaacactga ggcgatcgag
     4381 tgcatcacgc agggccggga gctggagcgg ccgcgcgcct gccctcctga tgtctacgcc
     4441 atcatgcgcg gctgctggca gcgtgagccg caacagcgcc tcagcatgaa ggatgtgcac
     4501 gcgcggctgc aggccttggc acaggcgcca ccgagttacc tggacgttct gggctagctc
     4561 gagccaacca ctgaggatct gtactttcag agcgataacg atggatccag tctccccggg
     4621 agctcatatg tgtcacttaa gtatatctag agtcgacctg caggcatgca agctgatccg
     4681 gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta
     4741 gcataacccc ttggggcggc cgcttcgagc agacatgata agatacattg atgagtttgg
     4801 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     4861 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
     4921 ttttatgttt caggttcagg gggagatgtg ggaggttttt ttaagcaagt aaaacctcta
     4981 caaatgtggt aaaatcgtat tctaatggag cctctttgcg cttgcgtttt cccttgtcca
     5041 gatagcccag tagctgacat tcatccgggg tcagcaccgt ttctgcggac tggctttcta
     5101 cgtgttccgc ttcctttagc agcccttgcg ccctgagtgc ttgcggcagc gtgagcttca
     5161 aaagaattgc cagctggggc gccctctggt aaggttggga agccctgcaa agtaaactgg
     5221 atggctttct tgccgccaag gatctgatgg cgcaggggat caagatctga tcaagagaca
     5281 ggatgacggt cgtttcgcat gcttgaacaa gatggattgc acgcaggttc tccggccgct
     5341 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     5401 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     5461 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc
     5521 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     5581 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     5641 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     5701 caccaagcga aacatcgcat cgagcgagca cgcactcgga tggaagccgg tcttgtcgat
     5761 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     5821 aaggcgcgta tgccggatgg tgaggatctc gtcgtgactc atggcgatgc ctgcttgccg
     5881 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     5941 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     6001 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     6061 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg
     6121 accaagcgac gcccaaccgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     6181 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     6241 taaaaaggcc gcgttgctgg cgtt
//
