LOCUS       sequence-259313-        5484 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  cytoplasmic DrTrkA expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5484)
  AUTHORS   Leopold AV, Chernov KG, Shemetov AA, Verkhusha VV
  TITLE     Neurotrophin receptor tyrosine kinases regulated with near-infrared 
            light.
  JOURNAL   Nat Commun. 2019 Mar 8;10(1):1129. doi: 10.1038/s41467-019-08988-3.
  PUBMED    30850602
REFERENCE   2  (bases 1 to 5484)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-03-8"; volume: "10(1):1129. doi"; pages: " 
            10.1038/s41467-019-08988-3"
COMMENT     Sequence Label: pCyto-Dr-TrkA
FEATURES             Location/Qualifiers
     source          1..5484
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(81..100)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    705..988
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
     promoter        1042..1093
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     1044..1064
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             3793..3813
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     primer_bind     complement(3944..3962)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    3999..4120
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4010..4029)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4064..4083
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             4519..5313
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MLEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4573..4592)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5183..5202
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      421 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
      481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatttcaag aagatccttt
      601 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      661 catgagatta tcaaaaagga tcttcaccta gatcctttta tagtccggaa atacaggaac
      721 gcacgctgga tggcccttcg ctgggatggt gaaaccatga aaaatggcag cttcagtgga
      781 ttaagtgggg gtaatgtggc ctgtaccctc tggttgcata ggtattcata cggttaaaat
      841 ttatcaggcg cgattgcggc agtttttcgg gtggtttgtt gccattttta cctgtctgct
      901 gccgtgatcg cgctgaacgc gttttagcgg tgcgtacaat taagggatta tggtaaatcc
      961 acttactgtc tgccctcgta gccatcgaga taaaccgcag tactccggcc acgatgcgtc
     1021 cggcgtagag gatcgagatc tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     1081 tataagcaga gctggtttag tgaaccgtca gatcactaga agcttatgag ccgggacccg
     1141 ttgccctttt ttccaccgct ttaccttggt ggcccggaaa ttaccaccga gaactgcgag
     1201 cgcgagccga ttcatattcc cggcagcatc cagccgcacg gcgccctgct cactgccgac
     1261 gggcacagcg gcgaggtgct ccagatgagc ctcaacgcgg ccacttttct gggacaggaa
     1321 cccacagtgc tgcgcggaca gaccctcgcc gcactgctgc ccgagcagtg gcccgcgctg
     1381 caagcggccc tgccccccgg ctgccccgac gccctgcaat accgcgcaac gctggactgg
     1441 cctgccgccg ggcacctttc gctgacggtg caccgggtcg gcgagttgct gattctggag
     1501 ttcgagccga cggaggcctg ggacagcacc gggccgcacg cgctgcgcaa cgcgatgttc
     1561 gcgctcgaaa gtgcccccaa cctgcgggcg ctggccgagg tggcgaccca gacggtccgc
     1621 gagctgacgg gctttgaccg ggtgatgctc tacaaatttg cccccgacgc caccggcgaa
     1681 gtgattgccg aggcccgccg tgaggggctg cacgcctttc tgggccaccg ttttcccgcg
     1741 tcggacattc cggcgcaggc ccgcgcgctc tacacccggc acctgctgcg cctgaccgcc
     1801 gacacccgcg ccgccgccgt gccgctcgat cccgtcctca acccgcagac gaatgcgccc
     1861 accccgctgg gcggcgccgt gctgcgcgcc acctcgccca tgcacatgca gtacctgcgg
     1921 aacatgggcg tcgggtcgag cctgtcggtg tcggtggtgg tcggcggcca gctctggggc
     1981 ctgatcgcct gccaccacca gacgccctac gtgttgccgc ccgacctgcg aaccacgctc
     2041 gaatacctgg gccgcttgct gagcctgcaa gttcaggtca aggaagcggc ggacgtggcg
     2101 gcctttcgcc agagcctgcg ggagcaccac gcgcgggtgg ccctcgcggc ggcgcactcg
     2161 ctctcgccgc acgacaccct cagtgacccg gcgcttgacc tgctgggcct gatgcgggcc
     2221 gggggcctga ttctgcgttt cgagggccgc tggcagacgt tgggtgaagt gccgcctgcc
     2281 ccggcggtgg acgcgctgct ggcgtggctc gaaacccagc cgggcgccct ggtccagacc
     2341 gacgcgctag gccaactgtg gcccgccggc gccgatctcg cccccagcgc agcgggcctg
     2401 ctcgccatca gcgtgggcga gggctggtcg gagtgcctcg tctggctgcg gcccgaactg
     2461 cggctggagg tcgcctgggg cggggccact cctgaccagg cgaaagacga cctcgggccg
     2521 cgccactcat tcgacaccta cctcgaagaa aaacgcggct acgccgagcc ctggcatccc
     2581 ggcgaaatcg aggaggcgca ggatctacgt gacacattga ccggggcgct gggcgaggca
     2641 gaggcagcag caaaggaggc agcagcaaag gaagcggcgg cgaaagaagc ggcggcgaaa
     2701 gcgaacaaat gtggacagag gagcaaattt gggatcaacc gccctgctgt gctggcgcca
     2761 gaggatgggc tggccatgtc cctacacttc atgacactgg gtggcagttc tctttcccct
     2821 actgagggca aaggctccgg actccagggc cacatcatgg agaacccaca gtacttcagt
     2881 gatacctgtg tccaccatat caagcgccag gacatcattc tcaagtggga gctaggggag
     2941 ggagcctttg gaaaggtctt tcttgctgag tgctacaacc ttctgaatga tcaggacaag
     3001 atgctggtgg ctgtcaaggc actgaaggag acatctgaga atgctcgtca ggacttccat
     3061 cgtgaggcag agctgctcac catgctacag caccaacaca tcgtacgctt ctttggagtc
     3121 tgcacggagg gtggcccatt gctcatggtc ttcgagtaca tgcgccatgg ggacctcaac
     3181 cgtttcctcc ggtcccacgg acctgatgca aaactgctgg ctggcggcga ggatgtggct
     3241 cctggtcctt tgggccttgg gcagcttctg gctgtggcta gccaggtggc tgctggtatg
     3301 gtgtacctag ccagcctgca ctttgtgcac cgggacctgg ccacacgcaa ctgtctggtg
     3361 ggtcagggac tagtggtcaa gattggagac ttcggcatga gcagggacat ctacagcaca
     3421 gactactacc gtgtgggagg tcggaccatg ctgcccatcc gctggatgcc tccagagagc
     3481 atcctctacc gcaagttcag caccgagagt gatgtgtgga gcttcggggt ggtgctctgg
     3541 gagatcttca cctatggaaa gcaaccctgg taccagctct ccaacactga ggcgatcgag
     3601 tgcatcacgc agggccggga gctggagcgg ccgcgcgcct gccctcctga tgtctacgcc
     3661 atcatgcgcg gctgctggca gcgtgagccg caacagcgcc tcagcatgaa ggatgtgcac
     3721 gcgcggctgc aggccttggc acaggcgcca ccgagttacc tggacgttct gggctagctc
     3781 gagccaacca ctgaggatct gtactttcag agcgataacg atggatccag tctccccggg
     3841 agctcatatg tgtcacttaa gtatatctag agtcgacctg caggcatgca agctgatccg
     3901 gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta
     3961 gcataacccc ttggggcggc cgcttcgagc agacatgata agatacattg atgagtttgg
     4021 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     4081 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
     4141 ttttatgttt caggttcagg gggagatgtg ggaggttttt ttaagcaagt aaaacctcta
     4201 caaatgtggt aaaatcgtat tctaatggag cctctttgcg cttgcgtttt cccttgtcca
     4261 gatagcccag tagctgacat tcatccgggg tcagcaccgt ttctgcggac tggctttcta
     4321 cgtgttccgc ttcctttagc agcccttgcg ccctgagtgc ttgcggcagc gtgagcttca
     4381 aaagaattgc cagctggggc gccctctggt aaggttggga agccctgcaa agtaaactgg
     4441 atggctttct tgccgccaag gatctgatgg cgcaggggat caagatctga tcaagagaca
     4501 ggatgacggt cgtttcgcat gcttgaacaa gatggattgc acgcaggttc tccggccgct
     4561 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     4621 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     4681 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc
     4741 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     4801 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     4861 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     4921 caccaagcga aacatcgcat cgagcgagca cgcactcgga tggaagccgg tcttgtcgat
     4981 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     5041 aaggcgcgta tgccggatgg tgaggatctc gtcgtgactc atggcgatgc ctgcttgccg
     5101 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     5161 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     5221 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     5281 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg
     5341 accaagcgac gcccaaccgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     5401 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     5461 taaaaaggcc gcgttgctgg cgtt
//
