LOCUS       sequence-259318-        5604 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Myr DrTrkA expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5604)
  AUTHORS   Leopold AV, Chernov KG, Shemetov AA, Verkhusha VV
  TITLE     Neurotrophin receptor tyrosine kinases regulated with near-infrared 
            light.
  JOURNAL   Nat Commun. 2019 Mar 8;10(1):1129. doi: 10.1038/s41467-019-08988-3.
  PUBMED    30850602
REFERENCE   2  (bases 1 to 5604)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-03-8"; volume: "10(1):1129. doi"; pages: " 
            10.1038/s41467-019-08988-3"
COMMENT     Sequence Label: pCMVd2-Dr-TrkB
FEATURES             Location/Qualifiers
     source          1..5604
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(81..100)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    705..988
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
     promoter        1042..1093
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     1044..1064
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             3913..3933
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     primer_bind     complement(4064..4082)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    4119..4240
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4130..4149)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4184..4203
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             4639..5433
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MLEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4693..4712)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5303..5322
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      421 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
      481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatttcaag aagatccttt
      601 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      661 catgagatta tcaaaaagga tcttcaccta gatcctttta tagtccggaa atacaggaac
      721 gcacgctgga tggcccttcg ctgggatggt gaaaccatga aaaatggcag cttcagtgga
      781 ttaagtgggg gtaatgtggc ctgtaccctc tggttgcata ggtattcata cggttaaaat
      841 ttatcaggcg cgattgcggc agtttttcgg gtggtttgtt gccattttta cctgtctgct
      901 gccgtgatcg cgctgaacgc gttttagcgg tgcgtacaat taagggatta tggtaaatcc
      961 acttactgtc tgccctcgta gccatcgaga taaaccgcag tactccggcc acgatgcgtc
     1021 cggcgtagag gatcgagatc tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     1081 tataagcaga gctggtttag tgaaccgtca gatcactaga agcttggtac catgggatgt
     1141 attaaatcaa aaaggaaaga cgctctgtac aaggaattca gtgctggtag tgctggtagt
     1201 gctggcaccg gtatgagccg ggacccgttg cccttttttc caccgcttta ccttggtggc
     1261 ccggaaatta ccaccgagaa ctgcgagcgc gagccgattc atattcccgg cagcatccag
     1321 ccgcacggcg ccctgctcac tgccgacggg cacagcggcg aggtgctcca gatgagcctc
     1381 aacgcggcca cttttttggg acaggaaccc acagtgctgc gcggacagac cctcgccgca
     1441 ctgctgcccg agcagtggcc cgcgctgcaa gcggccctgc cccccggctg ccccgacgcc
     1501 ctgcaatacc gcgcaacgct ggactggcct gccgccgggc acctttcgct gacggtgcac
     1561 cgggtcggcg agttgctgat tctggagttc gagccgacgg aggcctggga cagcaccggg
     1621 ccgcacgcgc tgcgcaacgc gatgttcgcg ttcgaaagtg cccccaacct gcgggcgctg
     1681 gccgaggtgg cgacccagac ggtccgcgag ctgacgggct ttgaccgggt gatgctctac
     1741 aaatttgccc ccgacgccac cggcgaagtg attgccgagg cccgccgtga ggggctgcac
     1801 gcctttctgg gccaccgttt tcccgcgtcg gacattccgg cgcaggcccg cgcgctctac
     1861 acccggcacc tgctgcgcct gaccgccgac acccgcgccg ccaccgtgcc gctcgatccc
     1921 gtcctcaacc cgcagacgaa tgcgcccacc ccgctgggcg gcgccgtgct gcgcgccacc
     1981 tcgcccatgc acatgcagta cctgcggaac atgggcgtcg ggtcgagcct gtcggtgtcg
     2041 gtggtggtcg gcggccagct ctggggcctg atcgcctgcc accaccagac gccctacgtg
     2101 ttgccgcccg acctgcgaac cacgctcgaa tacctgggcc gcttgctgag cctgcaagtt
     2161 caggtcaagg aagcggcgga cgtggcggcc tttcgccaga gcctgcggga gcaccacgcg
     2221 cgggtggccc tcgcggcggc gcactcgctc tcgccgcacg acaccctcag tgacccggcg
     2281 cttgacctgc tgggcctgat gcgggccggg ggcctgattc tgcgtttcga gggccgctgg
     2341 cagacgttgg gtgaagtgcc gcctgccccg gcggtggacg cgctgctggc gtggctcgaa
     2401 acccagccgg gcgccctggt ccagaccgac gcgctaggcc aactgtggct cgccggcgcc
     2461 gatctcgccc ccagcgcagc gggcctgctc gccatcagcg tgggcgaggg ctggtcggag
     2521 tgcctcgtct ggctgcggcc cgaactgcgg ctggaggtcg cctggggcgg ggccactcct
     2581 gaccaggcga aagacgacct cgggccgcgc cactcattcg acacctacct cgaagaaaaa
     2641 cgcggctacg ccgagccctg gcatcccggc gaaatcgagg aggcgcagga tctacgtgac
     2701 acattgaccg gggcgctggg cgaggcagag gcagcagcaa aggaggcagc agcaaaggaa
     2761 gcggcggcga aagaagcggc ggcgaaagcg aagttggcga gacattccaa gtttggcatg
     2821 aaaggcccag cttccgtcat cagcaacgac gatgactctg ccagccctct ccaccacatc
     2881 tccaacggga gcaacactcc gtcttcttcg gagggcgggc ccgatgctgt catcattggg
     2941 atgaccaaga tccctgtcat tgaaaacccc cagtacttcg gtatcaccaa cagccagctc
     3001 aagccggaca catttgttca gcacatcaag agacacaaca tcgttctgaa gagggagctt
     3061 ggagaaggag cctttgggaa agttttccta gcggagtgct ataacctctg ccccgagcag
     3121 gataagatcc tggtggccgt gaagacgctg aaggacgcca gcgacaatgc tcgcaaggac
     3181 tttcatcgcg aagccgagct gctgaccaac ctccagcacg agcacattgt caagttctac
     3241 ggtgtctgtg tggagggcga cccactcatc atggtctttg agtacatgaa gcacggggac
     3301 ctcaacaagt tccttagggc acacgggcca gatgcagtgc tgatggcaga gggtaacccg
     3361 cccaccgagc tgacgcagtc gcagatgctg cacatcgctc agcaaatcgc agcaggcatg
     3421 gtctacctgg catcccaaca cttcgtgcac cgagacctgg ccacccggaa ctgcttggta
     3481 ggagagaacc tgctggtgaa aattggggac ttcgggatgt cccgggatgt atacagcacc
     3541 gactactacc gggttggtgg ccacacaatg ttgcccatcc gatggatgcc tccagagagc
     3601 atcatgtaca ggaaattcac caccgagagt gacgtctgga gcctgggagt tgtgttgtgg
     3661 gagatcttca cctacggcaa gcagccctgg tatcagctat caaacaacga ggtgatagaa
     3721 tgcatcaccc agggcagagt ccttcagcgg cctcgcacgt gtccccagga ggtgtacgag
     3781 ctgatgctgg gatgctggca gcgggaacca cacacaagga agaacatcaa gaacatccac
     3841 acactccttc agaacttggc gaaggcgtcg cccgtctacc tggacatcct aggctagctc
     3901 gagccaacca ctgaggatct gtactttcag agcgataacg atggatccag tctccccggg
     3961 agctcatatg tgtcacttaa gtatatctag agtcgacctg caggcatgca agctgatccg
     4021 gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta
     4081 gcataacccc ttggggcggc cgcttcgagc agacatgata agatacattg atgagtttgg
     4141 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     4201 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
     4261 ttttatgttt caggttcagg gggagatgtg ggaggttttt ttaagcaagt aaaacctcta
     4321 caaatgtggt aaaatcgtat tctaatggag cctctttgcg cttgcgtttt cccttgtcca
     4381 gatagcccag tagctgacat tcatccgggg tcagcaccgt ttctgcggac tggctttcta
     4441 cgtgttccgc ttcctttagc agcccttgcg ccctgagtgc ttgcggcagc gtgagcttca
     4501 aaagaattgc cagctggggc gccctctggt aaggttggga agccctgcaa agtaaactgg
     4561 atggctttct tgccgccaag gatctgatgg cgcaggggat caagatctga tcaagagaca
     4621 ggatgacggt cgtttcgcat gcttgaacaa gatggattgc acgcaggttc tccggccgct
     4681 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     4741 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     4801 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc
     4861 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     4921 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     4981 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     5041 caccaagcga aacatcgcat cgagcgagca cgcactcgga tggaagccgg tcttgtcgat
     5101 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     5161 aaggcgcgta tgccggatgg tgaggatctc gtcgtgactc atggcgatgc ctgcttgccg
     5221 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     5281 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     5341 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     5401 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg
     5461 accaagcgac gcccaaccgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     5521 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     5581 taaaaaggcc gcgttgctgg cgtt
//
