LOCUS       sequence-25932-e         659 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral Luciferase Expression vector, CMV/TO promoter, Blast .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pQCXIB CMV/TO LUC (w431-1)
FEATURES             Location/Qualifiers
     source          1..659
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(48..72)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    113..131
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    134..152
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(154..357)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(183..203)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gaatggcgcc gggcctttct ttatgttttt ggcgtcttcc atggtggagc ctgctttttt
       61 gtacaaactt gttgatatct gcagaattcc accacactgg actagtcgac gatctctatc
      121 actgataggg agatctctat cactgatagg gagagctctg cttatataga cctcccaccg
      181 tacacgccta ccgcccattt gcgtcaatgg ggcggagttg ttacgacatt ttggaaagtc
      241 ccgttgattt tggtgccaaa acaaactccc attgacgtca atggggtgga gacttggaaa
      301 tccccgtgag tcaaaccgct atccacgccc attgatgtac tgccaaaacc gcatcaccat
      361 ggtaatagcg atgactaata cgtagatgta ctgccaagta ggaaagtccc ataaggtcat
      421 gtactgggca taatgccagg cgggccattt accgtcattg acgtcaatag ggggcgtact
      481 tggcatatga tacacttgat gtactgccaa gtgggcagtt taccgtaaat actccaccca
      541 ttgacgtcaa tggaaagtcc ctattggcgt tactatggga acatacgtca ttattgacgt
      601 caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa gttatgtaa
//
