LOCUS       sequence-25933-e         611 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral Luciferase expression vector, CMV/TO promoter, Puro .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pQCXP CMV/TO LUC (w432-1)
FEATURES             Location/Qualifiers
     source          1..611
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(45..69)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    110..128
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    131..149
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(151..354)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(180..200)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tggcgccggg cctttcttta tgtttttggc gtcttccatg gtggagcctg cttttttgta
       61 caaacttgtt gatatctgca gaattccacc acactggact agtcgacgat ctctatcact
      121 gatagggaga tctctatcac tgatagggag agctctgctt atatagacct cccaccgtac
      181 acgcctaccg cccatttgcg tcaatggggc ggagttgtta cgacattttg gaaagtcccg
      241 ttgattttgg tgccaaaaca aactcccatt gacgtcaatg gggtggagac ttggaaatcc
      301 ccgtgagtca aaccgctatc cacgcccatt gatgtactgc caaaaccgca tcaccatggt
      361 aatagcgatg actaatacgt agatgtactg ccaagtagga aagtcccata aggtcatgta
      421 ctgggcataa tgccaggcgg gccatttacc gtcattgacg tcaatagggg gcgtacttgg
      481 catatgatac acttgatgta ctgccaagtg ggcagtttac cgtaaatact ccacccattg
      541 acgtcaatgg aaagtcccta ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa
      601 tgggcggggg g
//
