LOCUS       sequence-259336-       11833 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of SOX4 after doxycycline administration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11833)
  AUTHORS   Doulatov S, Vo LT, Chou SS, Kim PG, Arora N, Li H, Hadland BK, 
            Bernstein ID, Collins JJ, Zon LI, Daley GQ
  TITLE     Induction of multipotential hematopoietic progenitors from human 
            pluripotent stem cells via respecification of lineage-restricted 
            precursors.
  JOURNAL   Cell Stem Cell. 2013 Oct 3;13(4):459-70. doi: 
            10.1016/j.stem.2013.09.002.
  PUBMED    24094326
REFERENCE   2  (bases 1 to 11833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2013.09"; journalName: "Cell Stem Cell"; date: 
            "2013-10-3- 3"; volume: "13"; issue: "4"; pages: "459-70"
COMMENT     Sequence Label: pINDUCER21-SOX4
FEATURES             Location/Qualifiers
     source          1..11833
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2230..2248
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2272..2290
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2314..2332
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2356..2374
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2407..2445
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2442..2466
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2575..2599
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4062..4086)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4139..4165
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        4385..5563
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4616..5554
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5511..5531
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    5633..5666
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(5673..6290,6291..6296,6297..6332,6333..6335,6336..6371,
                     6372..6374,6375..6410,6411..6419)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    6472..7045
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6639..6656)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6866..6885
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(7061..7063,7064..7066,7067..7780)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7061..7063,7064..7066,7067..7777)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7106..7127)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7367..7386)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7714..7735
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     7916..7935
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    8223..8811
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8276..8296)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8694..8705)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8886..9119
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9238..9256)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9324..9428
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9429..9497,9498..10289)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9647..9666)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10460..11048
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10949..10968
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11202..11219
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11459..11480
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11495..11500,11501..11518,11519..11525)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11533..11549
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11538..11560
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11557..11573
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11557..11573
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11623..11642
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactaggatc
     2221 ctttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     2281 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     2341 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagc tcggtacccg
     2401 ggtcgaggta ggcgtgtacg gtgggaggcc tatataagca gagctcgttt agtgaaccgt
     2461 cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga
     2521 tccagcctcc gcggccccga actagtccag tgtggtggaa ttctgcagat atcaacaagt
     2581 ttgtacaaaa aagcaggctt gaaggaattc ggtaccatgg tgcagcaaac caacaatgcc
     2641 gagaacacgg aagcgctgct ggccggcgag agctcggact cgggcgccgg cctcgagctg
     2701 ggaatcgcct cctcccccac gcccggctcc accgcctcca cgggcggcaa ggccgacgac
     2761 ccgagctggt gcaagacccc gagtgggcac atcaagcgac ccatgaacgc cttcatggtg
     2821 tggtcgcaga tcgagcggcg caagatcatg gagcagtcgc ccgacatgca caacgccgag
     2881 atctccaagc ggctgggcaa acgctggaag ctgctcaaag acagcgacaa gatccctttc
     2941 attcgagagg cggagcggct gcgcctcaag cacatggctg actaccccga ctacaagtac
     3001 cggcccagga agaaggtgaa gtccggcaac gccaactcca gctcctcggc cgccgcctcc
     3061 tccaagccgg gggagaaggg agacaaggtc ggtggcagtg gcgggggcgg ccatgggggc
     3121 ggcggcggcg gcgggagcag caacgcgggg ggaggaggcg gcggtgcgag tggcggcggc
     3181 gccaactcca aaccggcgca gaaaaagagc tgcggctcca aagtggcggg cggcgcgggc
     3241 ggtggggtta gcaaaccgca cgccaagctc atcctggcag gcggcggcgg cggcgggaaa
     3301 gcagcggctg ccgccgccgc ctccttcgcc gccgaacagg cgggggccgc cgccctgctg
     3361 cccctgggcg ccgccgccga ccaccactcg ctgtacaagg cgcggactcc cagcgcctcg
     3421 gcctccgcct cctcggcagc ctcggcctcc gcagcgctcg cggccccggg caagcacctg
     3481 gcggagaaga aggtgaagcg cgtctacctg ttcggcggcc tgggcacgtc gtcgtcgccc
     3541 gtgggcggcg tgggcgcggg agccgacccc agcgaccccc tgggcctgta cgaggaggag
     3601 ggcgcgggct gctcgcccga cgcgcccagc ctgagcggcc gcagcagcgc cgcctcgtcc
     3661 cccgccgccg gccgctcgcc cgccgaccac cgcggctacg ccagcctgcg cgccgcctcg
     3721 cccgccccgt ccagcgcgcc ctcgcacgcg tcctcctcgg cctcgtccca ctcctcctct
     3781 tcctcctcct cgggctcctc gtcctccgac gacgagttcg aagacgacct gctcgacctg
     3841 aaccccagct caaactttga gagcatgtcc ctgggcagct tcagttcgtc gtcggcgctc
     3901 gaccgggacc tggattttaa cttcgagccc ggctccggct cgcacttcga gttcccggac
     3961 tactgcacgc ccgaggtgag cgagatgatc tcgggagact ggctcgagtc cagcatctcc
     4021 aacctggttt tcacctacta gaagagcccg ggcgatcgcg aacccagctt tcttgtacaa
     4081 agtggttgat atccagcaca gtggcggccg ctcgagtcta gagggcccgc ggttcgaata
     4141 cccatacgac gtcccagact acgcttagta atgattaatt aaactagaaa ttctaccggg
     4201 taggggaggc gcttttccca aggcagtctg gagtaaccca cccaagatct ggcctccgcg
     4261 ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagccgcg taagatctgg
     4321 cctccgcgcc gggttttggc gcctcccgcg ggcgcccccc tcctcacggc gagcctagtc
     4381 gtgaggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
     4441 ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga
     4501 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
     4561 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag
     4621 tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg
     4681 aattacttcc acctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg
     4741 gtgggagagt tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc
     4801 ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc
     4861 tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt
     4921 ctggcaagat agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg
     4981 ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct
     5041 gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt
     5101 gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc
     5161 accagttgcg tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg
     5221 gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt
     5281 tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct
     5341 cgattagttc tcgagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc
     5401 gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat
     5461 gtaattctcc ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca
     5521 gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgaagcggcc gccatgctag
     5581 taaattgtcc gctaaattct ggccgttttt ggcttttttg ttagacggat ccataacttc
     5641 gtataatgta tgctatacga agttatgcca ccatgtctag actggacaag agcaaagtca
     5701 taaacggagc tctggaatta ctcaatggtg tcggtatcga aggcctgacg acaaggaaac
     5761 tcgctcaaaa gctgggagtt gagcagccta ccctgtactg gcacgtgaag aacaagcggg
     5821 ccctgctcga tgccctgcca atcgagatgc tggacaggca tcatacccac ttctgccccc
     5881 tggaaggcga gtcatggcaa gactttctgc ggaacaacgc caagtcatac cgctgtgctc
     5941 tcctctcaca tcgcgacggg gctaaagtgc atctcggcac ccgcccaaca gagaaacagt
     6001 acgaaaccct ggaaaatcag ctcgcgttcc tgtgtcagca aggcttctcc ctggagaacg
     6061 cactgtacgc tctgtccgcc gtgggccact ttacactggg ctgcgtattg gaggaacagg
     6121 agcatcaagt agcaaaagag gaaagagaga cacctaccac cgattctatg cccccacttc
     6181 tgagacaagc aattgagctg ttcgaccggc agggagccga acctgccttc cttttcggcc
     6241 tggaactaat catatgtggc ctggagaaac agctaaagtg cgaaagcggc gggccgaccg
     6301 acgcccttga cgattttgac ttagacatgc tcccagccga tgcccttgac gactttgacc
     6361 ttgatatgct gcctgctgac gctcttgacg attttgacct tgacatgctc cccgggtaac
     6421 taagtaagga tccgcggccg cactagagga attccgcccc tctccccccc ccccctctcc
     6481 ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt
     6541 ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg
     6601 ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg
     6661 tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc
     6721 tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca
     6781 aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag
     6841 ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa
     6901 ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt
     6961 tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt
     7021 tttcctttga aaaacacgat gataagctta ccggtccacc atggtgagca agggcgagga
     7081 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     7141 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     7201 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     7261 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     7321 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     7381 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     7441 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     7501 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     7561 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     7621 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     7681 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     7741 cgccgggatc actctcggca tggacgagct gtacaagtaa gcatgcccga cggcgaggat
     7801 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     7861 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     7921 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     7981 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     8041 ttctgatgta caagtaaagc ggccgcgact ctagatcata atcagccata ccacatttgt
     8101 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     8161 gaatgcaatt gttgttgttt agtccctccc aattcgatat caagcttatc gcgatagatc
     8221 ctaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     8281 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     8341 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     8401 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     8461 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     8521 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     8581 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     8641 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     8701 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     8761 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctgagatcc
     8821 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     8881 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
     8941 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     9001 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     9061 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     9121 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     9181 gagtgagagg cccgggttaa ttaaggaaag ggctagatca ttcttgaaga cgaaagggcc
     9241 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     9301 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     9361 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     9421 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     9481 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     9541 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     9601 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     9661 tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     9721 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     9781 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     9841 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     9901 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     9961 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
    10021 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
    10081 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
    10141 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
    10201 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
    10261 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
    10321 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
    10381 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    10441 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
    10501 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
    10561 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
    10621 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
    10681 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    10741 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    10801 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    10861 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    10921 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    10981 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    11041 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
    11101 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
    11161 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
    11221 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
    11281 gcagcaagct catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
    11341 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
    11401 gctccccgtg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
    11461 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
    11521 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gacatgatta
    11581 cgaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
    11641 tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca
    11701 aacttcccac cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa
    11761 cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta
    11821 caaacttagt agt
//
