LOCUS       sequence-259349-       12161 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pUC57mini ZM249M _env eGFP containing P90A mutant capsid sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12161)
  AUTHORS   Kim K, Dauphin A, Komurlu S, McCauley SM, Yurkovetskiy L, Carbone C,
            Diehl WE, Strambio-De-Castillia C, Campbell EM, Luban J
  TITLE     Cyclophilin A protects HIV-1 from restriction by human TRIM5alpha.
  JOURNAL   Nat Microbiol. 2019 Dec;4(12):2044-2051. doi: 
            10.1038/s41564-019-0592-5. Epub 2019 Oct 21.
  PUBMED    31636416
REFERENCE   2  (bases 1 to 12161)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41564-019-0592-5"; journalName: "Nat Microbiol"; date: 
            "2019-12"; volume: "4"; issue: "12"; pages: "2044-2051"
COMMENT     Sequence Label: pUC57mini ZM249M P90A _env eGFP
FEATURES             Location/Qualifiers
     source          1..12161
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    4340..4457
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      8283..8292
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(8289..8291,8292..8294,8295..9008)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8289..8291,8292..8294,8295..9005)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8334..8355)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8595..8614)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8942..8963
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             9660..9839
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(9925..10513)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10005..10024)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10684..11475,11476..11544))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11307..11326
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11545..11649)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gggtctctct tggtagacca gatctgagcc tgggagctct ctggctatct agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgctttaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga ccttttaggt agtgtggaaa atctctagca
      181 gtggcgcccg aacagggacc cgaaagcgaa agtagaacca gagaagatct ctcgacgcag
      241 gactcggctt gctgaagtgc actcggcaag aggcgagagc ggcggctggt gagtacgcca
      301 aattttattt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggaa aattagactc atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
      421 tataggctaa aacacatagt atgggcaagc agggagatgg aaagatttgc acttaaccct
      481 gagcttttag agtcagcaga tggctgtctg caaattctaa aacagataca accagctatt
      541 cagacaggaa cagaagaact taaatcatta tttaatacag tagcaactct ctattgtgta
      601 catcagagga tagagataaa agataccaag gaagccttag acaagataga ggaaatacaa
      661 aacaaaagtc agaaaaaagc acagcaggca gaagctgacc aaggaaaggt cagtcaaaat
      721 tatcctatag tacagaatct tcaagggcaa atggtacatc agcccctatc acctagaact
      781 ttaaatgcat gggtaaaagt aatagaagaa aaggctttca gcccagaagt aatacccatg
      841 tttacagcat tatcagaagg tgccacccca caagatttaa acaccatgtt aaatacagtg
      901 gggggacatc aagcagccat gcagatgtta aaagatacca tcaatgaaga agctgcagaa
      961 tgggataggc tacatccagt gcatgcaggt gccaatgcac caggccaaat gagggagcca
     1021 aggggaagtg acatagcagg aactactagt acccttcagg aacaaatagc ctggatgaca
     1081 agtaacccac ctattccagt aggagacatc tataaaagat ggataatcct aggattaaat
     1141 aaaatagtaa gaatgtatag cccagtcagc attttggaca taagacaagg accaaaagaa
     1201 ccctttagag actatgtaga taggttcttt aaaactttaa gagctgaaca agctacacaa
     1261 gaggtaaaaa attggatgac agacaccttg ttggttcaaa atgcgaaccc agattgtaag
     1321 accattttaa gagcattagg accaggggct acattagagg aaatgatgac agcatgtcag
     1381 ggagtgggag gacctggcca caaagcaagg gtgttggctg aggcaatgag ccaagtaaac
     1441 aatacaaaca tactgatgca gagaagcaat tttaagggcc ctaaaagaat tgttaaatgt
     1501 ttcaattgtg gcaaggaagg gcatatagcc agaaattgca gggcccctag gaaaaaaggc
     1561 tgttggaaat gtggaaagga aggacaccaa atgaaagact gtactgaaag gcaggctaat
     1621 tttttaggga aaatttggcc ttccaacaag gggaggccag gaaattttct ccagaacagg
     1681 ccagagccaa cagccccaca tttccttcag aacaggccag agccctcagc cccaccagca
     1741 gagagcttca ggttcgagga gacaaccccc gcttcgaagc aggagccgaa agacagggaa
     1801 cccttaactt ccctcaaatc actctttggc agcgacccct tgtctcaata aaagtagggg
     1861 gccaaataaa ggaggctctc ttagacacag gagcagatga tacagtatta gaagaaataa
     1921 atttgccagg aaaatggaaa ccaaaaatga tagggggaat tggaggtttt atcaaagtaa
     1981 gacagtatga tcaaataggt atagaaatct gtggaaaaaa ggctataggt acagtgttag
     2041 taggacccac acctgtcaac ataattggaa gaaacatgtt gactcagctt ggctgtactt
     2101 taaattttcc aattagtcct attgaaacta taccagtaaa attaaagcca ggaatggatg
     2161 ggccaaaggt taaacaatgg ccattgacag aagagaaaat aaaagcatta acagaaatct
     2221 gtagagaaat ggaaaaggag ggaaaaatta caaagattgg gcctgaaaac ccatacaata
     2281 ctccaatatt tgccataaaa aagaaagaca gtactaagtg gagaaaatta gtagatttta
     2341 gagaacttaa taaaagaact caagacttct gggaagttca attaggaata ccacacccag
     2401 cgggattaaa aaagaaaaaa tcagtaacag tgctagatgt gggggatgca tatttttcag
     2461 ttcctttaga tgaaaacttt aggaagtata ctgcattcac catacctagt ataaacaatg
     2521 aaacaccagg gattagatat caatacaatg tgctgccaca aggatggaaa ggatcaccag
     2581 caatatttca gagcagcatg acaaagatct tagagccctt tagggcaaaa aacccagaaa
     2641 tagttatcta tcaatatatg gatgacttgt atgtaggatc agatttagaa atagggcaac
     2701 atagagcaaa aatagaagag ttaagagaac acctattgag atggggattt accacaccag
     2761 acaaaaagca tcagaaggaa cccccgtttc tttggatggg gtacgaactc catcctgaca
     2821 aatggacagt acagcctata cagttgccaa aaaaggagag ctggactgtc aatgatatac
     2881 agaagttagt gggaaaatta aactgggcaa gtcagattta cccaggaatt aaagtaaagc
     2941 aactgtgtaa actccttagg ggagctaaag cactaacaga catagtacca ctaactgaag
     3001 aagcagaatt agaattggca gagaacaggg aagttctaaa agaaccagta catggagtat
     3061 attatgaccc atcaaaagac ctgatagctg aaatacagaa acaggggcag gatcaatgga
     3121 catatcaaat ttaccaagaa ccatttaaaa atctgaaaac ggggaagtat gcaaaaagga
     3181 ggactgccca cactaatgat gtaaaacagt taactgaggc ggtgcaaaaa atagccatgg
     3241 agagcatagt aatatgggga aagactccta aatttagact acccatccaa aaagaaacat
     3301 gggatacatg gtggacagac tattggcaag ccacctggat tcctgaatgg gagtttgtca
     3361 atacccctcc cctagtaaaa ttatggtacc agctagaaaa agaacccata ttaggagcag
     3421 aaactttcta tgtagatggg gcagctaata gagaaactaa aatagggaaa gcagggtatg
     3481 tcactgacag aggaagacag aaagttgttg ctctaactga aacaacaaat cagaagactg
     3541 agttacaagc aattcaaata gctttgcagg attcaggatc agaagtaaat atagtaacag
     3601 attcacaata tgcattagga atcattcaag cacaaccaga taagagtgaa tcagagttag
     3661 tcaatcaaat aatagaacag ttaataaaaa aggaaagggt ctacctgtca tgggtaccag
     3721 cacataaagg aattggagga aatgaacaag tagataaatt agtaagtaat ggaattagaa
     3781 aagtgctgtt tctagatgga atagataagg ctcaagaaga gcatgaaaga tatcacagta
     3841 attggagagc aatggctagt gagtttaacc tgccacccat agtggcaaaa gaaatagtag
     3901 ccagctgtga taaatgtcag ctaaaagggg aagccatgca tggacaagta gactgtagtc
     3961 cagggatatg gcaattagat tgtacacatt tagaaggaaa agtcatcctg gtagcagtcc
     4021 atgtagccag tggctatata gaagcagagg ttatcccagc agaaacagga caagaaacag
     4081 catattttat actaaaatta gcaggaagat ggccagtcaa aataatacat acagacaatg
     4141 gcagtaattt caccagtgct gcagttaagg cagcctgttg gtgggcaggt atccaacagg
     4201 agtttggaat tccctacaat ccccaaagtc agggagtagt agaatccatg aataaagaat
     4261 taaagaaaat catagggcag gtaagagatc aagctgagca ccttaagaca gcagtacaaa
     4321 tggcagtatt cattcacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
     4381 aaagaataat agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
     4441 taaaaattca aaattttcgg gtttattaca gagacagcag agaccctatt tggaaaggac
     4501 cagccaagct actctggaaa ggtgaagggg cagtagtaat acaagataat agtgacataa
     4561 aagtagtacc aaggaggaaa gtaaaaatca ttaaggatta tggaaaacag atggcaggtg
     4621 ctgattgtgt ggcaggtaga caggatgagg attagaacat ggaatagttt agtaaaacac
     4681 catatgtatg tttcaagaag agctaatgga tggttttata gacatcacta tgaaagtaga
     4741 catccaaaaa taagttcaga agtacacatc ccattagggg aagctagatt agtaataaaa
     4801 acatattggg ggttgcacac aggagaaaga gagtggcatt tgggtcatgg agtctccata
     4861 gaatggagac tgagaaggtt tagcacacaa gtagatcctg gcctggcaga ccaactaatt
     4921 cacatgcatt attttgattg ttttgcagac tctgccataa ggaaagccat attaggacag
     4981 atagttagtc ccaagtgtga ttatcaagca ggacacaata aggtaggatc cctgcaatat
     5041 ctagcactaa cagcattaat aaaaccaaag aagataaagc cacctctgcc tagtgtgcag
     5101 aaattagtag aggatagatg gaacaaaccc cagaagacca ggggccacag agggagccat
     5161 acaatgagtg ggcactagag cttttagaag aacttaagca ggaagctgtc agacactttc
     5221 ctagaccatg gctccatagc ttaggacaac atatctataa cacctatgga gatacttggg
     5281 caggagtcga agctataata agaatactgc aacagttact gtttattcat ttcagaattg
     5341 ggtgtcaaca tagcagaata ggcatcctgc gacagaggag aacaagaaat ggagccagta
     5401 gatcataatc tagacccctg gaaccatcca ggaagtcagc ctaaaactgc ttgtaatcaa
     5461 tgttattgta aacactgtag ctaccattgt caagtttgct ttctgacaaa aggcttaggc
     5521 atttcctatg gcaggaagaa gcggagacag cgacgaagta ctcctccaag cagtgaggat
     5581 catcaaaatc ttatatcaaa gcagtaagta gatgtaatga taagcttaat agaaaaagta
     5641 gattatagat taggagtagg agcattaata gtagcactga tcatagcaat agttgtgtgg
     5701 accatagcat atatagaata tagaaaattg ttaagacaaa ggaaaataga ctggttaatt
     5761 aaaagaatta gggaaagagc agaagacagt ggcaatgaga gtgatgggga tactgaggaa
     5821 ttgtcaacca tggtggatat ggagcatctt aggcttctgg atgttaatga attgtagtgg
     5881 aaacttgtgg gtcaccgtct attatggggt acctcgcgac acatgcctgt gtacccacag
     5941 accccaaccc acaagaaatg aatttggaaa atgtaacaga aaattttaac atgtggaaaa
     6001 atgatatggt ggatcagatg catgaggata taatcagttt atgggatcaa agcctaaagc
     6061 catgtgtaaa gttgacccca ctctgtgtca ctttaaactg taataatgtt aatgttaccc
     6121 acaacagtac ctacaacaat acagagggtg aacaaataaa aaattgctct ttcaatataa
     6181 ctacagagct aagagataag aaacagaaag tgtatgcact tttttataaa cttgacatat
     6241 tgccacttaa tgggaataat gatagtaatg agtatagatt aataaattgt aatacctcag
     6301 ccataacaca agcctgtcca aaggtctctt ttgacccaat tcctatacat tattgtgctc
     6361 cagctggcta tgcgattcta aagtgtaata ataagacatt caatggaaaa ggaccatgta
     6421 ataatgtcag cacagtgcaa tgtacacatg gaattaaacc agtggtatca actcaactac
     6481 tgttaaatgg cagcttagca gaaaaagaga taataattag atctgagaat ataacagaca
     6541 atgtcaaaat aataatagta catttaaatg aatctgtgga aattaactgt acacgaccca
     6601 ataataatac aagaaaaagt ataaggatag gaccaggaca aacattctat gcaacaggag
     6661 aaataatagg aaaaataaga gaagcacatt gtaacattag taaagaaaaa tggaataaaa
     6721 ctttactaag ggtagctaaa aaattaaggg aacactttcc tggtaaagca ataaaatttg
     6781 aaccatcttc aggaggggac ctagaaatta caacacatag ttttaattgt agaggagagt
     6841 ttttctattg cactacatcg aaactgttta atagtacata caatcctaat gacacagaaa
     6901 gtaattcaaa caattcaaac gagacactca cactcacatg caaaataaaa cagattataa
     6961 acatgtggca gggggtagga cgagcaatgt atgctccccc cattgaagga agcataacat
     7021 gtaactcaac tattacagga ttgctattga cacgtgatgg aggcagcaaa aataacacag
     7081 aagagatatt cagacctggg ggaggaaata tgaaggacaa ttggagaagt gaattatata
     7141 aatacaaagt ggtagaaatc aagccactag gagtagcgcc cactgaggca aaaaggagag
     7201 tggtggagag agaaaaaaga gcagtgggac tgggagctgt gttcttgggg ttcttaggag
     7261 cagcaggaag cactatgggc gcggcgtcaa taacgctgac ggtacaggcc agacaattat
     7321 tgtctggtat agtgcaacag caaaataatt tgctgagagc tatagaggcg caacaacata
     7381 tgttacaact cacagtctgg ggcattaagc agctccaggc aagagtcctg gccatagaaa
     7441 gatacctaaa ggatcaacag ctcctaggga tttggggctg ctctggaaaa ctcatctgca
     7501 ccacttctgt gccttggaac accagttgga gtaataagtc taaagcagaa atttgggata
     7561 atatgacctg gatgcagtgg gataaagaaa ttagtaatta tacacaaaca atatacaatt
     7621 tgcttgaaga atcgcagagc cagcaggaaa agaatgaaaa agatttacta gaattggaca
     7681 gctggaacaa tctgtggaat tggtttgaca tatcaaagtg gctgtggtat ataaaaatat
     7741 ttataatgat agtaggaggg ttgataggtt taagaataat ttttgctgtg ctatctatag
     7801 tgaatagagt taggcagggg tactcaccat tgtcttttca gatccttacc ccaaacccga
     7861 ggggacccga caggctcgga agaatcgaag aagaaggtgg agagcaagac agagacagat
     7921 ccgtgcgatt agcgaacgga ttcttagcac ttgcctggga agacctacgg aacctgtgcc
     7981 tcttcttcta ccaccgcttg agagacttca tcttgattgc agcgaggact gtggagctgc
     8041 tgagacagat cagcttcaag ggtctacaga gggggtggga agcccttaaa tatctgggaa
     8101 gccttgtgca gtattggagt caggagctaa aagagagtgc tattaatttg ctgaatacca
     8161 tagcaatagc agtagctgaa ggaacagata ggattataga agtagtacaa agaggtttta
     8221 gagctatcct caacgtaccc acaagaataa gacagggttt agaaagagct ttgctataag
     8281 cggccgccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     8341 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     8401 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     8461 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
     8521 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     8581 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     8641 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     8701 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     8761 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     8821 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     8881 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     8941 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     9001 acaagtaacc cgggctcgag acttggatag atttggagca gtcacaagca gcaatacagc
     9061 agccactaat gctgatgttg cctgtctgga agcacaagag gaggagggag aggtaggatt
     9121 tccagtcaga cctcaggtac ctctaagacc aatgacttat aagtcagcgg tcgatctcag
     9181 cttcttttta aaagaaaagg ggggactgga tgggctagtt tactccaaga aaagacaaga
     9241 tattcttgat ctgtgggtct ataacacaca aggcttcttc cctgactggc aaaactatac
     9301 accaggacca gggattcgat acccattgac ctttgggtgg tgctacaagt tagtgccagt
     9361 ggatccaagg gaagtggaag aggccaatga aggagagaac ttaagcttgt tacaccctat
     9421 gtgccagcat ggaatagagg acacagagag agaagtgctg atatggaagt ttgacagtca
     9481 cctagcacgc agacatatgg cccgcgagat acatccggag tactacaaag actgctgaca
     9541 cagaagggac tttccgctgg gactttccac tggggcgttc caggaggcgt ggtctgggcg
     9601 ggactgggag tggctgaccc tcagaagctg catataagca gctgcttttc gcttgtactg
     9661 ggtctctctt ggtagaccag atctgagcct gggagctctc tggctatcta gggaacccac
     9721 tgcttaagcc tcaataaagc ttgccttgag tgctttaagt agtgtgtgcc cgtctgttgt
     9781 gtgactctgg taactagaga tccctcagac cttttaggta gtgtggaaaa tctctagcag
     9841 cggccgaggc cggcctaggg cccacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     9901 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     9961 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
    10021 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
    10081 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
    10141 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
    10201 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
    10261 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
    10321 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
    10381 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
    10441 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
    10501 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
    10561 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
    10621 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
    10681 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
    10741 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
    10801 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
    10861 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
    10921 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
    10981 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
    11041 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
    11101 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
    11161 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
    11221 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
    11281 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
    11341 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
    11401 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
    11461 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
    11521 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
    11581 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
    11641 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc ccaatgatga tatcttaatt
    11701 aaacgcgttg gatgggctag tttactccaa gaaaagacaa gatattcttg atctgtgggt
    11761 ctataacaca caaggcttct tccctgactg gcaaaactat acaccaggac cagggattcg
    11821 atacccattg acctttgggt ggtgctacaa gttagtgcca gtggatccaa gggaagtgga
    11881 agaggccaat gaaggagaga acttaagctt gttacaccct atgtgccagc atggaataga
    11941 ggacacagag agagaagtgc tgatatggaa gtttgacagt cacctagcac gcagacatat
    12001 ggcccgcgag atacatccgg agtactacaa agactgctga cacagaaggg actttccgct
    12061 gggactttcc actggggcgt tccaggaggc gtggtctggg cgggactggg agtggctgac
    12121 cctcagaagc tgcatataag cagctgcttt tcgcttgtac t
//
