LOCUS       sequence-25935-e         765 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral RNAi empty control vector, 3'LTR insertion, Neo .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pLenti X2 Neo empty (w36-1)
FEATURES             Location/Qualifiers
     source          1..765
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      26..161
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    201..322
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(212..231)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     266..285
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             394..574
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     protein_bind    649..673
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(745..765)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 atagggcgaa ttgggtacgt ccctaggcct ccaaaaaagc ctcctcacta cttctggaat
       61 agctcagagg ccgaggcggc ctcggcctct gcataaataa aaaaaattag tcagccatgg
      121 ggcggagaat gggcggaact gggcggagtt aggggcggga tgggcggagt taggggcggg
      181 atagctagag ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat
      241 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
      301 tataagctgc aataaacaag ttcctctcac tctctgatat tcatttcttt gcaagttata
      361 aatactgaat aataagatga catgaactac tactgctaga gattttccac actgactaaa
      421 agggtctgag ggatctctag ttaccagagt cacacaacag acgggcacac actacttgaa
      481 gcactcaagg caagctttat tgaggcttaa gcagtgggtt ccctagttag ccagagagct
      541 cccaggctca gatctggtct aaccagagag acccagtaca agcaaaaagc agatcttgtc
      601 ttcgttggga gtgagctagt ccagtgtggt ggaattctgc agatatcaac aagtttgtac
      661 aaaaaagcag gctccaccat gggaaccaat tcagtcgact ggatccggta ccgaattcgc
      721 ggccgcactc gagatatcta gacccagctt tcttgtacaa agtgg
//
