LOCUS       sequence-259351-        6118 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivector for luciferase (control) knockdown and blasticidin 
            resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6118)
  AUTHORS   Kim K, Dauphin A, Komurlu S, McCauley SM, Yurkovetskiy L, Carbone C,
            Diehl WE, Strambio-De-Castillia C, Campbell EM, Luban J
  TITLE     Cyclophilin A protects HIV-1 from restriction by human TRIM5alpha.
  JOURNAL   Nat Microbiol. 2019 Dec;4(12):2044-2051. doi: 
            10.1038/s41564-019-0592-5. Epub 2019 Oct 21.
  PUBMED    31636416
REFERENCE   2  (bases 1 to 6118)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41564-019-0592-5"; journalName: "Nat Microbiol"; date: 
            "2019-12"; volume: "4"; issue: "12"; pages: "2044-2051"
COMMENT     Sequence Label: pABM-D4-miR30-L1221
FEATURES             Location/Qualifiers
     source          1..6118
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(132..152)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        137..466
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      317..452
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     379..398
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     enhancer        478..857
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        859..1057
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1012..1032
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             1075..1255
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1302..1427
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1924..2157
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2342..2386
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2684..2801
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2938..3345
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      3360..3369
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3550..3561
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     ncRNA           3859..3873
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     LTR             4007..4187
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(4258..4846)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4338..4357)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5017..5808,5809..5877))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5640..5659
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5878..5982)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6057..6076)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt
       61 gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat
      121 atacgcgctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
      181 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
      241 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
      301 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
      361 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
      421 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcgcgttgac
      481 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      541 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      601 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      661 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      721 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      781 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      841 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      901 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      961 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     1021 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
     1081 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     1141 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     1201 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1261 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1321 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1381 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1441 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1501 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1561 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1621 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1681 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1741 aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga
     1801 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     1861 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     1921 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     1981 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     2041 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     2101 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     2161 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     2221 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     2281 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     2341 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     2401 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     2461 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     2521 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     2581 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact
     2641 gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg
     2701 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     2761 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     2821 gcagagatcc agtttggtta attaactgca gccccgataa aataaaagat tttatttagt
     2881 ctccagaaaa aggggggaat gaaagacccc acctgtaggt ttggcaagct agctgcagta
     2941 acgccatttt gcaaggcatg gaaaaatacc aaaccaagaa tagagaagtt cagatcaagg
     3001 gcgggtacat gaaaatagct aacgttgggc caaacaggat atctgcggtg agcagtttcg
     3061 gccccggccc ggggccaaga acagatggtc accgcagttt cggccccggc ccgaggccaa
     3121 gaacagatgg tccccagata tggcccaacc ctcagcagtt tcttaagacc catcagatgt
     3181 ttccaggctc ccccaaggac ctgaaatgac cctgcgcctt atttgaatta accaatcagc
     3241 ctgcttctcg cttctgttcg cgcgcttctg cttcccgagc tctataaaag agctcacaac
     3301 ccctcactcg gcgcgccagt cctccgacag actgagtcgc ccgggggtct agaacgcgtg
     3361 ccgccatggc taaaccactg tcccaggaag aatccacact gatcgaacga gcaaccgcta
     3421 ctatcaactc aatcccaatc tccgaagact actctgtggc ctccgccgcc ctgagcagcg
     3481 acggacgaat cttcaccggc gtgaacgtct accactttac cggcggcccc tgcgctgagc
     3541 tggtggtcct gggaactgca gccgctgcag ccgctggaaa tctgacctgt atcgtggcta
     3601 tcgggaacga aaatagggga atcctgtctc catgcggccg gtgtagacag gtcctgctgg
     3661 atctgcatcc cgggatcaag gcaattgtga aagactcaga tggacagccc acagccgtcg
     3721 gcattcgcga gctgctgcct agcggctatg tgtgggaagg gtgagcgatc gccgcggccg
     3781 ccttcttaac ccaacagaag gctcgagaag gtatattgct gttgacagtg agcgcacaaa
     3841 cgctctcatc gacaagtagt gaagccacag atgtacttgt cgatgagagc gtttgtatgc
     3901 ctactgcctc ggacttcaag gggctagaat tcggcagctg tagatcttag ccacttttta
     3961 aaagaaaagg ggggactgga agggctaact gcatccggac tgtactgggt ctctctggtt
     4021 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     4081 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     4141 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg cccgtttcat
     4201 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     4261 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     4321 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     4381 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     4441 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     4501 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     4561 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     4621 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     4681 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     4741 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     4801 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     4861 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     4921 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     4981 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     5041 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     5101 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     5161 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     5221 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     5281 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     5341 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     5401 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     5461 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     5521 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     5581 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     5641 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     5701 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     5761 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     5821 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     5881 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     5941 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     6001 gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca
     6061 atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggagg
//
