LOCUS       sequence-25936-e         716 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral eGFP shRNA #1 (H1/TO promoter), 3'LTR insertion, 
            Neo .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pLenti X2 Neo/pTER shEGFP#1 (w21-2)
FEATURES             Location/Qualifiers
     source          1..716
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    25..43
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    complement(174..198)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(443..462)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 ctttggattt gggatcttat aagttcccta tcagtgatag agatcccgaa gcagcacgac
       61 ttcttcttca agagagaaga agtcgtgctg cttctttttg gaaaagcttg gtaccgagct
      121 cggatccact agtccagtgt ggtggaattc gcggccgcac tcgagatatc tagacccagc
      181 tttcttgtac aaagtggttg atatccagca cagtggcggc cgctcgagtc tagcccttcc
      241 agtcccccct tttcttttaa aaagtggcta agatctacag ctgccttgta agtcattggt
      301 cttaaaggta ccgggccctc gacggatccc ctcagaagaa ctcgtcaaga aggcgataga
      361 aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag cggtcagccc
      421 attcgccgcc aagctcttca gcaatatcac gggtagccaa cgctatgtcc tgatagcggt
      481 ccgccacacc cagccggcca cagtcgatga atccagaaaa gcggccattt tccaccatga
      541 tattcggcaa gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg ggcatgcgcg
      601 ccttgagcct ggcgaacagt tcggctggcg cgagcccctg atgctcttcg tccagatcat
      661 cctgatcgac aagaccggct tccatccgag tacgtgctcg ctcgatgcga tgtttc
//
