LOCUS       sequence-259369-       12592 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express CasRx under Ubiquitin-63E promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12592)
  AUTHORS   Buchman AB, Brogan DJ, Sun R, Yang T, Hsu PD, Akbari OS
  TITLE     Programmable RNA Targeting Using CasRx in Flies.
  JOURNAL   CRISPR J. 2020 Jun;3(3):164-176. doi: 10.1089/crispr.2020.0018.
  PUBMED    32584145
REFERENCE   2  (bases 1 to 12592)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/crispr.2020"; journalName: "CRISPR J"; date: "2020-06"; 
            volume: "3"; issue: "3"; pages: "164-176"
COMMENT     Sequence Label: OA-1050E (Ubiq-CasRx)
FEATURES             Location/Qualifiers
     source          1..12592
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(95..683)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(175..194)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(854..1645,1646..1714))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1477..1496
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1715..1819)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1887..1905
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1943..1965)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2065..2084
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2278..2300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2292..2309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2293..2309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   3013..3075
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    4109..4178
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     CDS             6259..6279
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6289..9186
                     /codon_start=1
                     /product="RNA-targeting Type VI CRISPR protein from 
                     Ruminococcus flavefaciens strain XPD3002 (Konermann et al.,
                     2018)"
                     /label=RfxCas13d
                     /translation="IEKKKSFAKGMGVKSTLVSGSKVYMTTFAEGSDARLEKIVEGDSI
                     RSVNEGEAFSAEMADKNAGYKIGNAKFSHPKGYAVVANNPLYTGPVQQDMLGLKETLEK
                     RYFGESADGNDNICIQVIHNILDIEKILAEYITNAAYAVNNISGLDKDIIGFGKFSTVY
                     TYDEFKDPEHHRAAFNNNDKLINAIKAQYDEFDNFLDNPRLGYFGQAFFSKEGRNYIIN
                     YGNECYDILALLSGLRHWVVHNNEEESRISRTWLYNLDKNLDNEYISTLNYLYDRITNE
                     LTNSFSKNSAANVNYIAETLGINPAEFAEQYFRFSIMKEQKNLGFNITKLREVMLDRKD
                     MSEIRKNHKVFDSIRTKVYTMMDFVIYRYYIEEDAKVAAANKSLPDNEKSLSEKDIFVI
                     NLRGSFNDDQKDALYYDEANRIWRKLENIMHNIKEFRGNKTREYKKKDAPRLPRILPAG
                     RDVSAFSKLMYALTMFLDGKEINDLLTTLINKFDNIQSFLKVMPLIGVNAKFVEEYAFF
                     KDSAKIADELRLIKSFARMGEPIADARRAMYIDAIRILGTNLSYDELKALADTFSLDEN
                     GNKLKKGKHGMRNFIINNVISNKRFHYLIRYGDPAHLHEIAKNEAVVKFVLGRIADIQK
                     KQGQNGKNQIDRYYETCIGKDKGKSVSEKVDALTKIITGMNYDQFDKKRSVIEDTGREN
                     AEREKFKKIISLYLTVIYHILKNIVNINARYVIGFHCVERDAQLYKEKGYDINLKKLEE
                     KGFSSVTKLCAGIDETAPDKRKDVEKEMAERAKESIDSLESANPKLYANYIKYSDEKKA
                     EEFTRQINREKAKTALNAYLRNTKWNVIIREDLLRIDNKTCTLFRNKAVHLEVARYVHA
                     YINDIAEVNSYFQLYHYIMQRIIMNERYEKSSGKVSEYFDAVNDEKKYNDRLLKLLCVP
                     FGYCIPRFKNLSIEALFDRNEAAKFDKEKKKVSGNS"
     CDS             9196..9216
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9226..9252
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(10487..10489,10490..10492,10493..11167)
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /translation="MVRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH
                     HLFL"
     primer_bind     complement(10670..10690)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     10888..10907
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     11082..11105
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    11281..11402
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11318..11337)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11372..11391
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   12070..12104
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(12285..12301)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12285..12301)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12298..12320)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12309..12325
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12333..12339,12340..12357,12358..12363))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12378..12399
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12516..12533)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
       61 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
      121 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
      181 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
      241 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
      301 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
      361 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
      421 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
      481 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
      541 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
      601 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
      661 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
      721 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
      781 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
      841 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
      901 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
      961 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     1021 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     1081 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     1141 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     1201 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     1261 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     1321 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     1381 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     1441 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     1501 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     1561 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     1621 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     1681 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     1741 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     1801 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     1861 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
     1921 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     1981 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     2041 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     2101 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc
     2161 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     2221 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     2281 agtcacgacg ttgtaaaacg acggccagtg ccaagctttg tttaaaaata taacaaaatt
     2341 gtgatcccac aaaatgaagt ggggcaaaat caaataatta atagtgtccg taaacttgtt
     2401 ggtcttcaac tttttgagga acacgttgga cggcaaatcc gtgactataa cacaagttga
     2461 tttaataatt ttagccaaca cgtcgggctg cgtgtttttt gccgacgcgt ctgtgtacac
     2521 gttgattaac tggtcgatta aactgttgaa ataatttaat ttttggttct tctttaaatc
     2581 tgtgatgaaa ttttttaaaa taactttaaa ttcttcattg gtaaaaaatg ccacgttttg
     2641 caacttgtga gggtctaata tgaggtcaaa ctcagtagga gttttatcca aaaaagaaaa
     2701 catgattacg tctgtacacg aacgcgtatt aacgcagagt gcaaagtata agagggttaa
     2761 aaaatatatt ttacgcacca tatacgcatc gggttgatat cgttaatatg gatcaatttg
     2821 aacagttgat taacgtgtct ctgctcaagt ctttgatcaa aacgcaaatc gacgaaaatg
     2881 tgtcggacaa tatcaagtcg atgagcgaaa aactaaaaag gctagaatac gacaatctca
     2941 cagacagcgt tgagatatac ggtattcacg acagcaggct gaataataaa aaaattagaa
     3001 actattattt aaccctagaa agataatcat attgtgacgt acgttaaaga taatcatgcg
     3061 taaaattgac gcatgtgttt tatcggtctg tatatcgagg tttatttatt aatttgaata
     3121 gatattaagt tttattatat ttacacttac atactaataa taaattcaac aaacaattta
     3181 tttatgttta tttatttatt aaaaaaaaac aaaaactcaa aatttcttct ataaagtaac
     3241 aaaactttta aacattctct cttttacaaa aataaactta ttttgtactt taaaaacagt
     3301 catgttgtat tataaaataa gtaattagct taacttatac ataatagaaa caaattatac
     3361 ttattagtca gtcagaaaca actttggcac atatcaatat tatgctctcg acaaataact
     3421 tttttgcatt ttttgcacga tgcatttgcc tttcgcctta ttttagaggg gcagtaagta
     3481 cagtaagtac gttttttcat tactggctct tcagtactgt catctgatgt accaggcact
     3541 tcatttggca aaatattaga gatattatcg cgcaaatatc tcttcaaagt aggagcttct
     3601 aaacgcttac gcataaacga tgacgtcagg ctcatgtaaa ggtttctcat aaattttttg
     3661 cgactttgaa ccttttctcc cttgctactg acattatggc tgtatataat aaaagaattt
     3721 atgcaggcaa tgtttatcat tccgtacaat aatgccatag gccacctatt cgtcttccta
     3781 ctgcaggtca tcacagaaca catttggtct agcgtgtcca ctccgccttt agtttgatta
     3841 taatacataa ccatttgcgg tttaccggta ctttcgttga tagaagcatc ctcatcacaa
     3901 gatgataata agtataccat cttagctggc ttcggtttat atgagacgag agtaaggggt
     3961 ccgtcaaaac aaaacatcga tgttcccact ggcctggagc gactgttttt cagtacttcc
     4021 ggtatctcgc gtttgtttga tcgcacggtt cccacaatgg ttaattcgag ctcgcccggg
     4081 gtcctaggtc gacgatgtag gtcacggtct cgaagccgcg gtgcgggtgc cagggcgtgc
     4141 ccttgggctc cccgggcgcg tactccacct cacccatctg gtccatcatg atgaacgggt
     4201 cgaggtggcg gtagttgatc ccggcgaacg cgcggcgcac cgggaagccc tcgccctcga
     4261 aaccgctggg cgcggtggtc acggtgagca cgggacgtgc gacggcgtcg gcgggtgcgg
     4321 atacgcgggg cagcgtcagc gggttctcga cggtcacggc gggcatgtcg acgcggccgc
     4381 cgcgcagatc gccgatgggg gtggcgccgg agggcacctt caccttgccg tacggcttgc
     4441 tgttcttcgc gttcaaaatc tccagctcca ttttgctttc ggtgcgcttg caatcagtac
     4501 tgtccaaaat cgaaaatcgc cgaaccgtag tgtgaccgtg cggggctctg cgaaaataaa
     4561 cttttttagg tatatggcca cacacgggga aagcacagtg gattatatgt tttaatatta
     4621 taatatgcag gttttcatta cttatccaga tgtaagccca cttaaagcga tttaacaatt
     4681 atttgccgaa agagtataaa caaatttcac ttaaaaatgg attaagaaaa gcttgtgtaa
     4741 gattatgcgc agcgttgcca gatagctcca tttaaaacac ttcaaaaaca ataagttttg
     4801 aaaatatata cataaatagc agtcgttgcc gcaacgctca acacatcaca cttttaaaac
     4861 accctttacc tacacagaat tactttttaa atttccagtc aagctgcgag tttcaaaatt
     4921 atagccggta gagaagacag tgctatttca aaagcaaact aacaagggtc ttaaattcca
     4981 aaacaccaat cctaacaagc cttggacttt tgtaagttta gatcaaaggt ggcattgcat
     5041 tcaatgtcat ggtaagaagt aggtcgtcta ggtagaaatc ctcattcagc cggtcaagtc
     5101 agtacgagaa aggtctcaat ttgaaattgt cttaaaaata ttttattgtt ttgtactgtg
     5161 gtgagtttaa acgaaaaaca caaaaaaaaa gtgatacaca gaaatcataa aaaattttaa
     5221 tacaaggtat tcgtacgtat caaaaacatt tcggcacaat tttttttctc tgtactaaag
     5281 tgttacgaac actacggtat tttttagtga ttttcaacgg acaccgaagg tatataaaca
     5341 gcgttcgcga acggtcgcct tcaaaaccaa ttgacatttg cagcagcaag tacaagtaga
     5401 aagtaaagcg caatcagcga aaaatttata cttaattgtt ggtgattaaa gtacaattaa
     5461 aagaacattc tcgaaagtca caagaaacgt aagtttttaa ctcgctgtta ccaattagta
     5521 ataagagcaa caagacgttg agtaatttca agaaaaactg catttcaagg tctttgttcg
     5581 gccatttttt ttttattcaa cgctctacgt aattacaaaa taagaaattg gcagccacgc
     5641 atcttgtttt cccaatgaat tggcatcaaa acgcaaacaa atctataaat aaaacttgcg
     5701 tgttgatttt cgccaagatt tattggcaaa ttgtgaaatt cgcagtgacg catttgaaaa
     5761 ttcgagaaat cacgaacgca ctcgagcatt tgtgtgcatg ttattagtta gttgtttagt
     5821 taattgaagt attttaccaa cgaaatccac ttatttttag ctgaaataga gtaggttgct
     5881 taaacaaagc cacgtctgaa aatttcttat tgcttgtagt tgtgacgtca ccatatacac
     5941 acaaaataat gtgtatgcat gcatttcagc tgtgtatata tacatgcaca cactcgcaac
     6001 acgaaaacga tgacgaagca acggaacaaa ggtttctcaa ctaccctttg ttccctgttt
     6061 cttcgctttc ctttgttcca atattcgtag agggttaata ggggtttctc aacaaagttg
     6121 gcgtcgataa ataagtttcc catttttatt ccccagccag gaagttagtt tcaatagttt
     6181 tgtaatttca acgaaactca tttgatttcg tactaatttt ccacatctct attttgaccc
     6241 gcagattaat taatgagccc caagaagaag agaaaggtgg aggccagcat cgaaaaaaaa
     6301 aagtccttcg ccaagggcat gggcgtgaag tccacactcg tgtccggctc caaagtgtac
     6361 atgacaacct tcgccgaagg cagcgacgcc aggctggaaa agatcgtgga gggcgacagc
     6421 atcaggagcg tgaatgaggg cgaggccttc agcgctgaaa tggccgataa aaacgccggc
     6481 tataagatcg gcaacgccaa attcagccat cctaagggct acgccgtggt ggctaacaac
     6541 cctctgtata caggacccgt ccagcaggat atgctcggcc tgaaggaaac tctggaaaag
     6601 aggtacttcg gcgagagcgc tgatggcaat gacaatattt gtatccaggt gatccataac
     6661 atcctggaca ttgaaaaaat cctcgccgaa tacattacca acgccgccta cgccgtcaac
     6721 aatatctccg gcctggataa ggacattatt ggattcggca agttctccac agtgtatacc
     6781 tacgacgaat tcaaagaccc cgagcaccat agggccgctt tcaacaataa cgataagctc
     6841 atcaacgcca tcaaggccca gtatgacgag ttcgacaact tcctcgataa ccccagactc
     6901 ggctatttcg gccaggcctt tttcagcaag gagggcagaa attacatcat caattacggc
     6961 aacgaatgct atgacattct ggccctcctg agcggactga ggcactgggt ggtccataac
     7021 aacgaagaag agtccaggat ctccaggacc tggctctaca acctcgataa gaacctcgac
     7081 aacgaataca tctccaccct caactacctc tacgacagga tcaccaatga gctgaccaac
     7141 tccttctcca agaactccgc cgccaacgtg aactatattg ccgaaactct gggaatcaac
     7201 cctgccgaat tcgccgaaca atatttcaga ttcagcatta tgaaagagca gaaaaacctc
     7261 ggattcaata tcaccaagct cagggaagtg atgctggaca ggaaggatat gtccgagatc
     7321 aggaaaaatc ataaggtgtt cgactccatc aggaccaagg tctacaccat gatggacttt
     7381 gtgatttata ggtattacat cgaagaggat gccaaggtgg ctgccgccaa taagtccctc
     7441 cccgataatg agaagtccct gagcgagaag gatatctttg tgattaacct gaggggctcc
     7501 ttcaacgacg accagaagga tgccctctac tacgatgaag ctaatagaat ttggagaaag
     7561 ctcgaaaata tcatgcacaa catcaaggaa tttaggggaa acaagacaag agagtataag
     7621 aagaaggacg cccctagact gcccagaatc ctgcccgctg gccgtgatgt ttccgccttc
     7681 agcaaactca tgtatgccct gaccatgttc ctggatggca aggagatcaa cgacctcctg
     7741 accaccctga ttaataaatt cgataacatc cagagcttcc tgaaggtgat gcctctcatc
     7801 ggagtcaacg ctaagttcgt ggaggaatac gcctttttca aagactccgc caagatcgcc
     7861 gatgagctga ggctgatcaa gtccttcgct agaatgggag aacctattgc cgatgccagg
     7921 agggccatgt atatcgacgc catccgtatt ttaggaacca acctgtccta tgatgagctc
     7981 aaggccctcg ccgacacctt ttccctggac gagaacggaa acaagctcaa gaaaggcaag
     8041 cacggcatga gaaatttcat tattaataac gtgatcagca ataaaaggtt ccactacctg
     8101 atcagatacg gtgatcctgc ccacctccat gagatcgcca aaaacgaggc cgtggtgaag
     8161 ttcgtgctcg gcaggatcgc tgacatccag aaaaaacagg gccagaacgg caagaaccag
     8221 atcgacaggt actacgaaac ttgtatcgga aaggataagg gcaagagcgt gagcgaaaag
     8281 gtggacgctc tcacaaagat catcaccgga atgaactacg accaattcga caagaaaagg
     8341 agcgtcattg aggacaccgg cagggaaaac gccgagaggg agaagtttaa aaagatcatc
     8401 agcctgtacc tcaccgtgat ctaccacatc ctcaagaata ttgtcaatat caacgccagg
     8461 tacgtcatcg gattccattg cgtcgagcgt gatgctcaac tgtacaagga gaaaggctac
     8521 gacatcaatc tcaagaaact ggaagagaag ggattcagct ccgtcaccaa gctctgcgct
     8581 ggcattgatg aaactgcccc cgataagaga aaggacgtgg aaaaggagat ggctgaaaga
     8641 gccaaggaga gcattgacag cctcgagagc gccaacccca agctgtatgc caattacatc
     8701 aaatacagcg acgagaagaa agccgaggag ttcaccaggc agattaacag ggagaaggcc
     8761 aaaaccgccc tgaacgccta cctgaggaac accaagtgga atgtgatcat cagggaggac
     8821 ctcctgagaa ttgacaacaa gacatgtacc ctgttcagaa acaaggccgt ccacctggaa
     8881 gtggccaggt atgtccacgc ctatatcaac gacattgccg aggtcaattc ctacttccaa
     8941 ctgtaccatt acatcatgca gagaattatc atgaatgaga ggtacgagaa aagcagcgga
     9001 aaggtgtccg agtacttcga cgctgtgaat gacgagaaga agtacaacga taggctcctg
     9061 aaactgctgt gtgtgccttt cggctactgt atccccaggt ttaagaacct gagcatcgag
     9121 gccctgttcg ataggaacga ggccgccaag ttcgacaagg agaaaaagaa ggtgtccggc
     9181 aattccggat ccggacctaa gaaaaagagg aaggtggcgg ccgcttaccc atacgatgtt
     9241 ccagattacg cttaagctag ctagaatgaa tcgtttttaa aataacaaat caattgtttt
     9301 ataatattcg tacgattctt tgattatgta ataaaatgtg atcattagga agattacgaa
     9361 aaatataaaa aatatgagtt ctgtgtgtat aacaaatgct gtaaacgcca caattgtgtt
     9421 tgttgcaaat aaacccatga ttatttgatt aaaattgttg ttttctttgt tcatagacaa
     9481 tagtgtgttt tgcctaaacg tgtactgcat aaactccatg cgagtgtata gcgagctagt
     9541 ggctaacgct tgccccacca aagtagattc gtcaaaatcc tcaatttcat caccctcctc
     9601 caagtttaac atttggccgt cggaattaac ttctaaagat gccacataat ctaataaatg
     9661 aaatagagat tcaaacgtgg cgtcatcgtc cgtttcgacc atttccgaaa agaactcggg
     9721 cataaactct atgatttctc tggacgtggt gttgtcgaaa ctctcaaagt acgcagtcag
     9781 gaacgtgcgc gacatgtcgt cgggaaactc gcgcggaaac atgttgttgt aaccgaacgg
     9841 gtcccatagc gccaaaacca aatctgccag cgtcaataga atgagcacga tgccgacaat
     9901 ggagctggct tggatagcga ttcgagttaa cggccggggc gcgcccatac tcggtggcct
     9961 ccccaccacc aacttttttg cactgcaaaa aaacacgctt ttgcacgcgg gcccatacat
    10021 agtacaaact ctacgtttcg tagactattt tacataaata gtctacaccg ttgtatacgc
    10081 tccaaataca ctaccacaca ttgaaccttt ttgcagtgca aaaaagtacg tgtcggcagt
    10141 cacgtaggcc ggccttatcg ggtcgcgtcc tgtcacgtac gaatcacatt atcggaccgg
    10201 acgagtgttg tcttatcgtg acaggacgcc agcttcctgt gttgctaacc gcagccggac
    10261 gcaactcctt atcggaacag gacgcgcctc catatcagcc gcgcgttatc tcatgcgcgt
    10321 gaccggacac gaggcgcccg tcccgcttat cgcgcctata aatacagccc gcaacgatct
    10381 ggtaaacaca gttgaacagc atctgttaca gcgacacaac atgagccggt ccaacaacgc
    10441 caacgcgccc acgccatcca accgccgccg caacctgtct ctggtgatgg tgcgctcctc
    10501 caagaacgtc atcaaggagt tcatgcgctt caaggtgcgc atggagggca ccgtgaacgg
    10561 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggcc acaacaccgt
    10621 gaagctgaag gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccccca
    10681 gttccagtac ggctccaagg tgtacgtgaa gcaccccgcc gacatccccg actacaagaa
    10741 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt
    10801 ggtgaccgtg acccaggact cctccctgca ggacggctgc ttcatctaca aggtgaagtt
    10861 catcggcgtg aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
    10921 ggcctccacc gagcgcctgt acccccgcga cggcgtgctg aagggcgaga tccacaaggc
    10981 cctgaagctg aaggacggcg gccactacct ggtggagttc aagtccatct acatggccaa
    11041 gaagcccgtg cagctgcccg gctactacta cgtggactcc aagctggaca tcacctccca
    11101 caacgaggac tacaccatcg tggagcagta cgagcgcacc gagggccgcc accacctgtt
    11161 cctgtaggac tctagatcat aatcagccat accacatttg tagaggtttt acttgcttta
    11221 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
    11281 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
    11341 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
    11401 taaagcttct aggccggccg atctcggatc tgacaatgtt cagtgcagag actcggctac
    11461 gcctcgtgga ctttgaagtt gaccaacaat gtttattctt acctctaata gtcctctgtg
    11521 gcaaggtcaa gattctgtta gaagccaatg aagaacctgg ttgttcaata acattttgtt
    11581 cgtctaatat ttcactaccg cttgacgttg gctgcacttc atgtacctca tctataaacg
    11641 cttcttctgt atcgctctgg acgtcatctt cacttacgtg atctgatatt tcactgtcag
    11701 aatcctcacc aacaagctcg tcatcgcttt gcagaagagc agagaggata tgctcatcgt
    11761 ctaaagaact acccatttta ttatatatta gtcacgatat ctataacaag aaaatatata
    11821 tataataagt tatcacgtaa gtagaacatg aaataacaat ataattatcg tatgagttaa
    11881 atcttaaaag tcacgtaaaa gataatcatg cgtcattttg actcacgcgg tcgttatagt
    11941 tcaaaatcag tgacacttac cgcattgaca agcacgcctc acgggagctc caagcggcga
    12001 ctgagatgtc ctaaatgcac agcgacggat tcgcgctatt tagaaagaga gagcaatatt
    12061 tcaagaatgc atgcgtcaat tttacgcaga ctatctttct agggttaaaa aagatttgcg
    12121 ctttactcga cctaaacttt aaacacgtca tagaatcttc gtttgacaaa aaccacattg
    12181 tggccaagct gtgtgacgcg acgcgcgcta aagaatggca aaccaagtcg cgcgagcgtc
    12241 gactctagag gatccccggg taccgagctc gaattcgtaa tcatgtcata gctgtttcct
    12301 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
    12361 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
    12421 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
    12481 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
    12541 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gt
//
