LOCUS       sequence-25938-e         208 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral eGFP shRNA #2 (H1/TO promoter), 3'LTR insertion, 
            Neo .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 208)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 208)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pLenti X2 Neo/pTER shEGFP#2 (w22-2)
FEATURES             Location/Qualifiers
     source          1..208
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    26..44
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    complement(176..200)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tctttggatt tgggatctta taagttccct atcagtgata gagatccccg ctggagtaca
       61 actacaactt caagagagtt gtagttgtac tccagctttt tggaaaagct tggtaccgag
      121 ctcggatcca ctagtccagt gtggtggaat tcgcggccgc actcgagata tctagaccca
      181 gctttcttgt acaaagtggt tgatatcc
//
