LOCUS       sequence-259401-       11327 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express dCasRx under UASt promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11327)
  AUTHORS   Buchman AB, Brogan DJ, Sun R, Yang T, Hsu PD, Akbari OS
  TITLE     Programmable RNA Targeting Using CasRx in Flies.
  JOURNAL   CRISPR J. 2020 Jun;3(3):164-176. doi: 10.1089/crispr.2020.0018.
  PUBMED    32584145
REFERENCE   2  (bases 1 to 11327)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/crispr.2020"; journalName: "CRISPR J"; date: "2020-06"; 
            volume: "3"; issue: "3"; pages: "164-176"
COMMENT     Sequence Label: OA-1050S (UASt-dCasRx)
FEATURES             Location/Qualifiers
     source          1..11327
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(95..683)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(175..194)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(854..1645,1646..1714))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1477..1496
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1715..1819)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1887..1905
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1943..1965)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2065..2084
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2278..2300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2292..2309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2293..2309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   3013..3075
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    4109..4178
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     protein_bind    4408..4441
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    4454..4548
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    4564..4597
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    4610..4704
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        4728..4966
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     4913..4934
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             4994..5014
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5024..7921
                     /codon_start=1
                     /product="RNA-targeting Type VI CRISPR protein from 
                     Ruminococcus flavefaciens strain XPD3002 (Konermann et al.,
                     2018)"
                     /label=RfxCas13d
                     /translation="IEKKKSFAKGMGVKSTLVSGSKVYMTTFAEGSDARLEKIVEGDSI
                     RSVNEGEAFSAEMADKNAGYKIGNAKFSHPKGYAVVANNPLYTGPVQQDMLGLKETLEK
                     RYFGESADGNDNICIQVIHNILDIEKILAEYITNAAYAVNNISGLDKDIIGFGKFSTVY
                     TYDEFKDPEHHRAAFNNNDKLINAIKAQYDEFDNFLDNPRLGYFGQAFFSKEGRNYIIN
                     YGNECYDILALLSGLAHWVVANNEEESRISRTWLYNLDKNLDNEYISTLNYLYDRITNE
                     LTNSFSKNSAANVNYIAETLGINPAEFAEQYFRFSIMKEQKNLGFNITKLREVMLDRKD
                     MSEIRKNHKVFDSIRTKVYTMMDFVIYRYYIEEDAKVAAANKSLPDNEKSLSEKDIFVI
                     NLRGSFNDDQKDALYYDEANRIWRKLENIMHNIKEFRGNKTREYKKKDAPRLPRILPAG
                     RDVSAFSKLMYALTMFLDGKEINDLLTTLINKFDNIQSFLKVMPLIGVNAKFVEEYAFF
                     KDSAKIADELRLIKSFARMGEPIADARRAMYIDAIRILGTNLSYDELKALADTFSLDEN
                     GNKLKKGKHGMRNFIINNVISNKRFHYLIRYGDPAHLHEIAKNEAVVKFVLGRIADIQK
                     KQGQNGKNQIDRYYETCIGKDKGKSVSEKVDALTKIITGMNYDQFDKKRSVIEDTGREN
                     AEREKFKKIISLYLTVIYHILKNIVNINARYVIGFHCVERDAQLYKEKGYDINLKKLEE
                     KGFSSVTKLCAGIDETAPDKRKDVEKEMAERAKESIDSLESANPKLYANYIKYSDEKKA
                     EEFTRQINREKAKTALNAYLRNTKWNVIIREDLLRIDNKTCTLFANKAVALEVARYVHA
                     YINDIAEVNSYFQLYHYIMQRIIMNERYEKSSGKVSEYFDAVNDEKKYNDRLLKLLCVP
                     FGYCIPRFKNLSIEALFDRNEAAKFDKEKKKVSGNS"
     CDS             7931..7951
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7961..7987
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(9222..9224,9225..9227,9228..9902)
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /translation="MVRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH
                     HLFL"
     primer_bind     complement(9405..9425)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     9623..9642
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     9817..9840
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    10016..10137
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10053..10072)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10107..10126
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   10805..10839
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(11020..11036)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11020..11036)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11033..11055)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11044..11060
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11068..11074,11075..11092,11093..11098))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11113..11134
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11251..11268)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
       61 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
      121 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
      181 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
      241 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
      301 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
      361 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
      421 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
      481 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
      541 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
      601 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
      661 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
      721 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
      781 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
      841 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
      901 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
      961 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     1021 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     1081 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     1141 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     1201 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     1261 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     1321 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     1381 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     1441 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     1501 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     1561 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     1621 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     1681 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     1741 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     1801 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     1861 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
     1921 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     1981 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     2041 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     2101 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc
     2161 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     2221 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     2281 agtcacgacg ttgtaaaacg acggccagtg ccaagctttg tttaaaaata taacaaaatt
     2341 gtgatcccac aaaatgaagt ggggcaaaat caaataatta atagtgtccg taaacttgtt
     2401 ggtcttcaac tttttgagga acacgttgga cggcaaatcc gtgactataa cacaagttga
     2461 tttaataatt ttagccaaca cgtcgggctg cgtgtttttt gccgacgcgt ctgtgtacac
     2521 gttgattaac tggtcgatta aactgttgaa ataatttaat ttttggttct tctttaaatc
     2581 tgtgatgaaa ttttttaaaa taactttaaa ttcttcattg gtaaaaaatg ccacgttttg
     2641 caacttgtga gggtctaata tgaggtcaaa ctcagtagga gttttatcca aaaaagaaaa
     2701 catgattacg tctgtacacg aacgcgtatt aacgcagagt gcaaagtata agagggttaa
     2761 aaaatatatt ttacgcacca tatacgcatc gggttgatat cgttaatatg gatcaatttg
     2821 aacagttgat taacgtgtct ctgctcaagt ctttgatcaa aacgcaaatc gacgaaaatg
     2881 tgtcggacaa tatcaagtcg atgagcgaaa aactaaaaag gctagaatac gacaatctca
     2941 cagacagcgt tgagatatac ggtattcacg acagcaggct gaataataaa aaaattagaa
     3001 actattattt aaccctagaa agataatcat attgtgacgt acgttaaaga taatcatgcg
     3061 taaaattgac gcatgtgttt tatcggtctg tatatcgagg tttatttatt aatttgaata
     3121 gatattaagt tttattatat ttacacttac atactaataa taaattcaac aaacaattta
     3181 tttatgttta tttatttatt aaaaaaaaac aaaaactcaa aatttcttct ataaagtaac
     3241 aaaactttta aacattctct cttttacaaa aataaactta ttttgtactt taaaaacagt
     3301 catgttgtat tataaaataa gtaattagct taacttatac ataatagaaa caaattatac
     3361 ttattagtca gtcagaaaca actttggcac atatcaatat tatgctctcg acaaataact
     3421 tttttgcatt ttttgcacga tgcatttgcc tttcgcctta ttttagaggg gcagtaagta
     3481 cagtaagtac gttttttcat tactggctct tcagtactgt catctgatgt accaggcact
     3541 tcatttggca aaatattaga gatattatcg cgcaaatatc tcttcaaagt aggagcttct
     3601 aaacgcttac gcataaacga tgacgtcagg ctcatgtaaa ggtttctcat aaattttttg
     3661 cgactttgaa ccttttctcc cttgctactg acattatggc tgtatataat aaaagaattt
     3721 atgcaggcaa tgtttatcat tccgtacaat aatgccatag gccacctatt cgtcttccta
     3781 ctgcaggtca tcacagaaca catttggtct agcgtgtcca ctccgccttt agtttgatta
     3841 taatacataa ccatttgcgg tttaccggta ctttcgttga tagaagcatc ctcatcacaa
     3901 gatgataata agtataccat cttagctggc ttcggtttat atgagacgag agtaaggggt
     3961 ccgtcaaaac aaaacatcga tgttcccact ggcctggagc gactgttttt cagtacttcc
     4021 ggtatctcgc gtttgtttga tcgcacggtt cccacaatgg ttaattcgag ctcgcccggg
     4081 gtcctaggtc gacgatgtag gtcacggtct cgaagccgcg gtgcgggtgc cagggcgtgc
     4141 ccttgggctc cccgggcgcg tactccacct cacccatctg gtccatcatg atgaacgggt
     4201 cgaggtggcg gtagttgatc ccggcgaacg cgcggcgcac cgggaagccc tcgccctcga
     4261 aaccgctggg cgcggtggtc acggtgagca cgggacgtgc gacggcgtcg gcgggtgcgg
     4321 atacgcgggg cagcgtcagc gggttctcga cggtcacggc gggcatgtcg acgcggccgc
     4381 aaccaacaac actagtagta ccagcttata acttcgtata atgtatgcta tacgaagtta
     4441 tctgcaggca ggtcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact
     4501 gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg gagactccca
     4561 tggataactt cgtataatgt atgctatacg aagttatgga tccgcaggtc ggagtactgt
     4621 cctccgagcg gagtactgtc ctccgagcgg agtactgtcc tccgagcgga gtactgtcct
     4681 ccgagcggag tactgtcctc cgagcggaga ctcgtcgaca gcgagcgccg gagtataaat
     4741 agaggcgctt cgtctacgga gcgacaattc aattcaaaca agcaaagtga acacgtcgct
     4801 aagcgaaagc taagcaaata aacaagcgca gctgaacaag ctaaacaatc tgcagtaaag
     4861 tgcaagttaa agtgaatcaa ttaaaagtaa ccagcaacca agtaaatcaa ctgcaactac
     4921 tgaaatctgc caagaagtaa ttattgaata caagaagaga actctgaata gggaattggg
     4981 tttaaacatg agccccaaga agaagagaaa ggtggaggcc agcatcgaaa aaaaaaagtc
     5041 cttcgccaag ggcatgggcg tgaagtccac actcgtgtcc ggctccaaag tgtacatgac
     5101 aaccttcgcc gaaggcagcg acgccaggct ggaaaagatc gtggagggcg acagcatcag
     5161 gagcgtgaat gagggcgagg ccttcagcgc tgaaatggcc gataaaaacg ccggctataa
     5221 gatcggcaac gccaaattca gccatcctaa gggctacgcc gtggtggcta acaaccctct
     5281 gtatacagga cccgtccagc aggatatgct cggcctgaag gaaactctgg aaaagaggta
     5341 cttcggcgag agcgctgatg gcaatgacaa tatttgtatc caggtgatcc ataacatcct
     5401 ggacattgaa aaaatcctcg ccgaatacat taccaacgcc gcctacgccg tcaacaatat
     5461 ctccggcctg gataaggaca ttattggatt cggcaagttc tccacagtgt atacctacga
     5521 cgaattcaaa gaccccgagc accatagggc cgctttcaac aataacgata agctcatcaa
     5581 cgccatcaag gcccagtatg acgagttcga caacttcctc gataacccca gactcggcta
     5641 tttcggccag gcctttttca gcaaggaggg cagaaattac atcatcaatt acggcaacga
     5701 atgctatgac attctggccc tcctgagcgg actggcgcac tgggtggtcg ctaacaacga
     5761 agaagagtcc aggatctcca ggacctggct ctacaacctc gataagaacc tcgacaacga
     5821 atacatctcc accctcaact acctctacga caggatcacc aatgagctga ccaactcctt
     5881 ctccaagaac tccgccgcca acgtgaacta tattgccgaa actctgggaa tcaaccctgc
     5941 cgaattcgcc gaacaatatt tcagattcag cattatgaaa gagcagaaaa acctcggatt
     6001 caatatcacc aagctcaggg aagtgatgct ggacaggaag gatatgtccg agatcaggaa
     6061 aaatcataag gtgttcgact ccatcaggac caaggtctac accatgatgg actttgtgat
     6121 ttataggtat tacatcgaag aggatgccaa ggtggctgcc gccaataagt ccctccccga
     6181 taatgagaag tccctgagcg agaaggatat ctttgtgatt aacctgaggg gctccttcaa
     6241 cgacgaccag aaggatgccc tctactacga tgaagctaat agaatttgga gaaagctcga
     6301 aaatatcatg cacaacatca aggaatttag gggaaacaag acaagagagt ataagaagaa
     6361 ggacgcccct agactgccca gaatcctgcc cgctggccgt gatgtttccg ccttcagcaa
     6421 actcatgtat gccctgacca tgttcctgga tggcaaggag atcaacgacc tcctgaccac
     6481 cctgattaat aaattcgata acatccagag cttcctgaag gtgatgcctc tcatcggagt
     6541 caacgctaag ttcgtggagg aatacgcctt tttcaaagac tccgccaaga tcgccgatga
     6601 gctgaggctg atcaagtcct tcgctagaat gggagaacct attgccgatg ccaggagggc
     6661 catgtatatc gacgccatcc gtattttagg aaccaacctg tcctatgatg agctcaaggc
     6721 cctcgccgac accttttccc tggacgagaa cggaaacaag ctcaagaaag gcaagcacgg
     6781 catgagaaat ttcattatta ataacgtgat cagcaataaa aggttccact acctgatcag
     6841 atacggtgat cctgcccacc tccatgagat cgccaaaaac gaggccgtgg tgaagttcgt
     6901 gctcggcagg atcgctgaca tccagaaaaa acagggccag aacggcaaga accagatcga
     6961 caggtactac gaaacttgta tcggaaagga taagggcaag agcgtgagcg aaaaggtgga
     7021 cgctctcaca aagatcatca ccggaatgaa ctacgaccaa ttcgacaaga aaaggagcgt
     7081 cattgaggac accggcaggg aaaacgccga gagggagaag tttaaaaaga tcatcagcct
     7141 gtacctcacc gtgatctacc acatcctcaa gaatattgtc aatatcaacg ccaggtacgt
     7201 catcggattc cattgcgtcg agcgtgatgc tcaactgtac aaggagaaag gctacgacat
     7261 caatctcaag aaactggaag agaagggatt cagctccgtc accaagctct gcgctggcat
     7321 tgatgaaact gcccccgata agagaaagga cgtggaaaag gagatggctg aaagagccaa
     7381 ggagagcatt gacagcctcg agagcgccaa ccccaagctg tatgccaatt acatcaaata
     7441 cagcgacgag aagaaagccg aggagttcac caggcagatt aacagggaga aggccaaaac
     7501 cgccctgaac gcctacctga ggaacaccaa gtggaatgtg atcatcaggg aggacctcct
     7561 gagaattgac aacaagacat gtaccctgtt cgcaaacaag gccgtcgccc tggaagtggc
     7621 caggtatgtc cacgcctata tcaacgacat tgccgaggtc aattcctact tccaactgta
     7681 ccattacatc atgcagagaa ttatcatgaa tgagaggtac gagaaaagca gcggaaaggt
     7741 gtccgagtac ttcgacgctg tgaatgacga gaagaagtac aacgataggc tcctgaaact
     7801 gctgtgtgtg cctttcggct actgtatccc caggtttaag aacctgagca tcgaggccct
     7861 gttcgatagg aacgaggccg ccaagttcga caaggagaaa aagaaggtgt ccggcaattc
     7921 cggatccgga cctaagaaaa agaggaaggt ggcggccgct tacccatacg atgttccaga
     7981 ttacgcttaa gctagctaga atgaatcgtt tttaaaataa caaatcaatt gttttataat
     8041 attcgtacga ttctttgatt atgtaataaa atgtgatcat taggaagatt acgaaaaata
     8101 taaaaaatat gagttctgtg tgtataacaa atgctgtaaa cgccacaatt gtgtttgttg
     8161 caaataaacc catgattatt tgattaaaat tgttgttttc tttgttcata gacaatagtg
     8221 tgttttgcct aaacgtgtac tgcataaact ccatgcgagt gtatagcgag ctagtggcta
     8281 acgcttgccc caccaaagta gattcgtcaa aatcctcaat ttcatcaccc tcctccaagt
     8341 ttaacatttg gccgtcggaa ttaacttcta aagatgccac ataatctaat aaatgaaata
     8401 gagattcaaa cgtggcgtca tcgtccgttt cgaccatttc cgaaaagaac tcgggcataa
     8461 actctatgat ttctctggac gtggtgttgt cgaaactctc aaagtacgca gtcaggaacg
     8521 tgcgcgacat gtcgtcggga aactcgcgcg gaaacatgtt gttgtaaccg aacgggtccc
     8581 atagcgccaa aaccaaatct gccagcgtca atagaatgag cacgatgccg acaatggagc
     8641 tggcttggat agcgattcga gttaacggcc ggggcgcgcc catactcggt ggcctcccca
     8701 ccaccaactt ttttgcactg caaaaaaaca cgcttttgca cgcgggccca tacatagtac
     8761 aaactctacg tttcgtagac tattttacat aaatagtcta caccgttgta tacgctccaa
     8821 atacactacc acacattgaa cctttttgca gtgcaaaaaa gtacgtgtcg gcagtcacgt
     8881 aggccggcct tatcgggtcg cgtcctgtca cgtacgaatc acattatcgg accggacgag
     8941 tgttgtctta tcgtgacagg acgccagctt cctgtgttgc taaccgcagc cggacgcaac
     9001 tccttatcgg aacaggacgc gcctccatat cagccgcgcg ttatctcatg cgcgtgaccg
     9061 gacacgaggc gcccgtcccg cttatcgcgc ctataaatac agcccgcaac gatctggtaa
     9121 acacagttga acagcatctg ttacagcgac acaacatgag ccggtccaac aacgccaacg
     9181 cgcccacgcc atccaaccgc cgccgcaacc tgtctctggt gatggtgcgc tcctccaaga
     9241 acgtcatcaa ggagttcatg cgcttcaagg tgcgcatgga gggcaccgtg aacggccacg
     9301 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggccacaac accgtgaagc
     9361 tgaaggtgac caagggcggc cccctgccct tcgcctggga catcctgtcc ccccagttcc
     9421 agtacggctc caaggtgtac gtgaagcacc ccgccgacat ccccgactac aagaagctgt
     9481 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga
     9541 ccgtgaccca ggactcctcc ctgcaggacg gctgcttcat ctacaaggtg aagttcatcg
     9601 gcgtgaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct
     9661 ccaccgagcg cctgtacccc cgcgacggcg tgctgaaggg cgagatccac aaggccctga
     9721 agctgaagga cggcggccac tacctggtgg agttcaagtc catctacatg gccaagaagc
     9781 ccgtgcagct gcccggctac tactacgtgg actccaagct ggacatcacc tcccacaacg
     9841 aggactacac catcgtggag cagtacgagc gcaccgaggg ccgccaccac ctgttcctgt
     9901 aggactctag atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
     9961 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
    10021 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
    10081 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaaag
    10141 cttctaggcc ggccgatctc ggatctgaca atgttcagtg cagagactcg gctacgcctc
    10201 gtggactttg aagttgacca acaatgttta ttcttacctc taatagtcct ctgtggcaag
    10261 gtcaagattc tgttagaagc caatgaagaa cctggttgtt caataacatt ttgttcgtct
    10321 aatatttcac taccgcttga cgttggctgc acttcatgta cctcatctat aaacgcttct
    10381 tctgtatcgc tctggacgtc atcttcactt acgtgatctg atatttcact gtcagaatcc
    10441 tcaccaacaa gctcgtcatc gctttgcaga agagcagaga ggatatgctc atcgtctaaa
    10501 gaactaccca ttttattata tattagtcac gatatctata acaagaaaat atatatataa
    10561 taagttatca cgtaagtaga acatgaaata acaatataat tatcgtatga gttaaatctt
    10621 aaaagtcacg taaaagataa tcatgcgtca ttttgactca cgcggtcgtt atagttcaaa
    10681 atcagtgaca cttaccgcat tgacaagcac gcctcacggg agctccaagc ggcgactgag
    10741 atgtcctaaa tgcacagcga cggattcgcg ctatttagaa agagagagca atatttcaag
    10801 aatgcatgcg tcaattttac gcagactatc tttctagggt taaaaaagat ttgcgcttta
    10861 ctcgacctaa actttaaaca cgtcatagaa tcttcgtttg acaaaaacca cattgtggcc
    10921 aagctgtgtg acgcgacgcg cgctaaagaa tggcaaacca agtcgcgcga gcgtcgactc
    10981 tagaggatcc ccgggtaccg agctcgaatt cgtaatcatg tcatagctgt ttcctgtgtg
    11041 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
    11101 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
    11161 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
    11221 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
    11281 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggt
//
