LOCUS       sequence-259405-        5164 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV vector expressing Cre from rTH promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5164)
  TITLE     AAV plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5164)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: AAV.rTH.PI.Cre.SV40
FEATURES             Location/Qualifiers
     source          1..5164
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    63..84
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(99..104,105..122,123..129)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    137..153
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     142..164
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     161..177
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     161..177
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   215..344
                     /label=AAV2 ITR
     intron          817..949
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     932..951
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        994..1012
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1061..2092
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     polyA_signal    2145..2266
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2156..2175)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2210..2229
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   2428..2557
                     /label=AAV2 ITR
     primer_bind     complement(2576..2593)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2576..2592)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2585..2607)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2734..3189
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2821..2840)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3031..3052
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3215..3319
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3320..3388,3389..4180)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3538..3557)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4351..4939
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4840..4859
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
       61 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
      121 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
      181 gattacgcca gatttaatta aggccttaat taggctgcgc gctcgctcgc tcactgaggc
      241 cgcccgggca aagcccgggc gtcgggcgac ctttggtcgc ccggcctcag tgagcgagcg
      301 agcgcgcaga gagggagtgg ccaactccat cactaggggt tccttgtagt taatgattaa
      361 cccgccatgc tacttatcta cgtagccatg ctctaggaag atcaattcct agcggtctcc
      421 tgtcccacag aataccagcc agcccctgcc ctacgtcgtg cctcgggctg agggtgattc
      481 agaggcaggt gcctgtgaca gtggatgcaa ttagatctaa tgggacggag gcctttctcg
      541 tcgccctcgc tccatgccca cccccgcctc cctcaggcac agcaggcgtg gagaggatgc
      601 gcaggaggta ggaggtgggg gacccagagg ggctttgacg tcagcctggc ctttaaagag
      661 ggcgcctgcc tggcgagggc tgtggagaca gaactcggga ccaccagaag ctttattgcg
      721 gtagtttatc acagttaaat tgctaacgca gtcagtgctt ctgacacaac agtctcgaac
      781 ttaagctgca gaagttggtc gtgaggcact gggcaggtaa gtatcaaggt tacaagacag
      841 gtttaaggag accaatagaa actgggcttg tcgagacaga gaagactctt gcgtttctga
      901 taggcaccta ttggtcttac tgacatccac tttgcctttc tctccacagg tgtccactcc
      961 cagttcaatt acagctctta aggctagagt acttaatacg actcactata ggctagtcga
     1021 gaattgtaca ctttacttaa aaccattatc tgagtgtgaa atgtccaatt tactgaccgt
     1081 acaccaaaat ttgcctgcat taccggtcga tgcaacgagt gatgaggttc gcaagaacct
     1141 gatggacatg ttcagggatc gccaggcgtt ttctgagcat acctggaaaa tgcttctgtc
     1201 cgtttgccgg tcgtgggcgg catggtgcaa gttgaataac cggaaatggt ttcccgcaga
     1261 acctgaagat gttcgcgatt atcttctata tcttcaggcg cgcggtctgg cagtaaaaac
     1321 tatccagcaa catttgggcc agctaaacat gcttcatcgt cggtccgggc tgccacgacc
     1381 aagtgacagc aatgctgttt cactggttat gcggcggatc cgaaaagaaa acgttgatgc
     1441 cggtgaacgt gcaaaacagg ctctagcgtt cgaacgcact gatttcgacc aggttcgttc
     1501 actcatggaa aatagcgatc gctgccagga tatacgtaat ctggcatttc tggggattgc
     1561 ttataacacc ctgttacgta tagccgaaat tgccaggatc agggttaaag atatctcacg
     1621 tactgacggt gggagaatgt taatccatat tggcagaacg aaaacgctgg ttagcaccgc
     1681 aggtgtagag aaggcactta gcctgggggt aactaaactg gtcgagcgat ggatttccgt
     1741 ctctggtgta gctgatgatc cgaataacta cctgttttgc cgggtcagaa aaaatggtgt
     1801 tgccgcgcca tctgccacca gccagctatc aactcgcgcc ctggaaggga tttttgaagc
     1861 aactcatcga ttgatttacg gcgctaagga tgactctggt cagagatacc tggcctggtc
     1921 tggacacagt gcccgtgtcg gagccgcgcg agatatggcc cgcgctggag tttcaatacc
     1981 ggagatcatg caagctggtg gctggaccaa tgtaaatatt gtcatgaact atatccgtaa
     2041 cctggatagt gaaacagggg caatggtgcg cctgctggaa gatggcgatt agccattaac
     2101 gcggcgtggt acctctagag tcgggccgct tcgagcagac atgataagat acattgatga
     2161 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     2221 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     2281 cattcatttt atgtttcagg ttcaggggga gatgtgggag gttttttaaa gcaagtaaaa
     2341 cctctacaaa tgtggtaaaa tcgataagga tcttcctaga gcatggctac gtagataagt
     2401 agcatggcgg gttaatcatt aactacaagg aacccctagt gatggagttg gccactccct
     2461 ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct
     2521 ttgcccgggc ggcctcagtg agcgagcgag cgcgcagcct taattaacct aattcactgg
     2581 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     2641 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     2701 cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg
     2761 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     2821 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     2881 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     2941 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     3001 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     3061 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     3121 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
     3181 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     3241 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     3301 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     3361 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     3421 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     3481 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     3541 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     3601 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     3661 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     3721 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     3781 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     3841 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     3901 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     3961 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     4021 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     4081 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     4141 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     4201 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     4261 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     4321 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     4381 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     4441 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     4501 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     4561 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     4621 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     4681 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     4741 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     4801 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     4861 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     4921 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     4981 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     5041 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     5101 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     5161 gatt
//
