LOCUS       sequence-25941-e         763 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral Luciferase shRNA (H1/TO promoter), 3'LTR 
            insertion, Neo .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pLenti X2 Neo/pTER shLUC (w181-1)
FEATURES             Location/Qualifiers
     source          1..763
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    14..32
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    complement(165..189)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(434..453)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 ggatcttata agttccctat cagtgataga gatcccctta cgctgagtac ttcgagtgtg
       61 ctgtcctcga agtactcagc gtaagttttt ggaaaagctt ggtaccgagc tcggatccac
      121 tagtccagtg tggtggaatt cgcggccgca ctcgagatat ctagacccag ctttcttgta
      181 caaagtggtt gatatccagc acagtggcgg ccgctcgagt ctagcccttc cagtcccccc
      241 ttttctttta aaaagtggct aagatctaca gctgccttgt aagtcattgg tcttaaaggt
      301 accgggccct cgacggatcc cctcagaaga actcgtcaag aaggcgatag aaggcgatgc
      361 gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc
      421 caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcgg tccgccacac
      481 ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg atattcggca
      541 agcaggcatc gccatgggtc acgacgagat cctcgccgtc gggcatgcgc gccttgagcc
      601 tggcgaacag ttcggctggc gcgagcccct gatgctcttn ntccanatca tcctgatcga
      661 cnngaccngc ttccatccna gtacntgctc gctcnatgcn atgtttcnct ngntggtcna
      721 atggncangt agccggatca agcgtatgca ggccnncgca ttg
//
