LOCUS       sequence-259412-        3225 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Part plasmid for loxP-KIURA3-loxP, Type 6 (yeast marker) part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   An-Adirekkun JM, Stewart CJ, Geller SH, Patel MT, Melendez J, Oakes 
            BL, Noyes MB, McClean MN
  TITLE     A yeast optogenetic toolkit (yOTK) for gene expression control in 
            Saccharomyces cerevisiae.
  JOURNAL   Biotechnol Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234.
  PUBMED    31788779
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234."
COMMENT     Sequence Label: pMM0519
FEATURES             Location/Qualifiers
     source          1..3225
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(462..495)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      complement(630..1218)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(710..729)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1400..2059
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1466..1485)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      2130..2164
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     protein_bind    complement(2202..2235)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 agcacacggt gaatatacta agggtaccgt tgatattgca aagagtgata aagatttcgt
       61 tattgggttc attgctcaga acgatatggg aggaagagaa gaagggtttg attggctaat
      121 catgacccca ggtgtaggtt tagacgacaa aggcgatgca ttgggtcagc agtacagaac
      181 cgtcgacgaa gttgtaagtg gtggatcaga tatcatcatt gttggcagag gacttttcgc
      241 caagggtaga gatcctaagg ttgaaggtga aagatacaga aatgctggat gggaagcgta
      301 ccaaaagaga atcagcgctc cccattaatt atacaggaaa cttaatagaa caaatcacat
      361 atttaatcta atagccacct gcattggcac ggtgcaacac tacttcaact tcatcttaca
      421 aaaagatcac gtgatctgtt gtattgggat ctctagacct aataacttcg tatagcatac
      481 attatacgaa gttatattaa gggttgtcga cctgctgtat gagaccgact acggttatcc
      541 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
      601 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
      661 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
      721 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
      781 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
      841 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
      901 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
      961 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     1021 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     1081 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     1141 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     1201 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     1261 aacgaaaact cacgttaagg gattttggtc atgattgatc gggcacgtaa gaggttccaa
     1321 ctttcaccat aatgaaataa gatcactacc gggcgtattt tttgagttat cgagattttc
     1381 aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc
     1441 ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa
     1501 ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa
     1561 gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaatttcg
     1621 tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt
     1681 tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg
     1741 gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt
     1801 ccctaaaggg tttattgaga atatgttttt cgtttcagcc aatccctggg tgagtttcac
     1861 cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg
     1921 caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc
     1981 cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga
     2041 gtggcagggc ggggcgtaat ttgatatcga gctcgcttgg actcctgttg atagatccag
     2101 taatgacctc agaactccat ctggatttgt tcagaacgct cggttgccgc cgggcgtttt
     2161 ttattggtct ggtctcaact cagtggatct gatatcacct aataacttcg tatagcatac
     2221 attatacgaa gttatattaa gggttctcga gagctcgttt tatttaggtt ctatcgagga
     2281 gaaaaagcga caagaagaga tagaccatgg ataaatgatt atgttctaaa cactcctcag
     2341 aagctcatcg aactgtcatc ctgcgtgaag attaaaatcc aacttagaaa tttcgagctt
     2401 acggagacaa tcatatggga gaagcaattg gaagatagaa aaaaggtact cggtacataa
     2461 atatatgtga ttctgggtag aagatcggtc tgcattggat ggtggtaacg cattttttta
     2521 cacacattac ttgcctcgag catcaaatgg tggttattcg tggatctata tcacgtgatt
     2581 tgcttaagaa ttgtcgttca tggtgacact tttagctttg acatgattaa gctcatctca
     2641 attgatgtta tctaaagtca tttcaactat ctaagatgtg gttgtgattg ggccattttg
     2701 tgaaagccag tacgccagcg tcaatacact cccgtcaatt agttgcacca tgtccacaaa
     2761 atcatatacc agtagagctg agactcatgc aagtccggtt gcatcgaaac ttttacgttt
     2821 aatggatgaa aagaagacca atttgtgtgc ttctcttgac gttcgttcga ctgatgagct
     2881 attgaaactt gttgaaacgt tgggtccata catttgcctt ttgaaaacac acgttgatat
     2941 cttggatgat ttcagttatg agggtactgt cgttccattg aaagcattgg cagagaaata
     3001 caagttcttg atatttgagg acagaaaatt cgccgatatc ggtaacacag tcaaattaca
     3061 atatacatcg ggcgtttacc gtatcgcaga atggtctgat atcaccaacg cccacggggt
     3121 tactggtgct ggtattgttg ctggcttgaa acaaggtgcg caagaggtca ccaaagaacc
     3181 aaggggatta ttgatgcttg ctgaattgtc ttccaagggt tctct
//
