LOCUS       sequence-259416-        3898 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pREV1-mRUBY2 cassette plasmid flanked by ConL2 and ConRE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   An-Adirekkun JM, Stewart CJ, Geller SH, Patel MT, Melendez J, Oakes 
            BL, Noyes MB, McClean MN
  TITLE     A yeast optogenetic toolkit (yOTK) for gene expression control in 
            Saccharomyces cerevisiae.
  JOURNAL   Biotechnol Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234.
  PUBMED    31788779
REFERENCE   2  (bases 1 to 3898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234."
COMMENT     Sequence Label: pMM0625
FEATURES             Location/Qualifiers
     source          1..3898
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             943..1653
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     terminator      1669..1856
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1724..1748)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(join(2173..2964,2965..3033))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2796..2815
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3034..3138)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3241..3829
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3730..3749
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcgttatccc ctgattctgt ggataaccgt gcggccgccc ctgaattcgc atctagaata
       61 aatcgcagcc aagtgagtga atagatgacg caccacggtc agacacggcc acatcgtatc
      121 tcacaggagc aagcgcgata ggagcactca cacatagtac ggtgatccgc tgactccttt
      181 gcccaaataa gacgtgagcc cgtctcagat gcacacactg gcttaagatg acaacggtgt
      241 tgttatccga tacaaccgga tatttttctt ttaatgagtc taaaccgtga tagcttcagg
      301 ttaatacaat caaaaaaagc tcaaatattc ttttaatgcc gcgttcacag attccaattg
      361 aatacaacta ggtagttcat tatatgaagc ctttgctact atttttcact atagtctgcc
      421 ttcaccttaa tgcagacatc cacatatttt aatcacttta aaataaaaag gaagatatat
      481 tagaagctat gatccaatct gtaagccaga ttaaaattca cgaactcttc tttcatttga
      541 attgaatgct ttgagttggg gtagattatc gcaaattact catcacattt attgactacg
      601 aacttgctga tgtccttttt ttatttatat ttttcttcag tgaagcgatt ttttttttac
      661 acagaccaag acggaaaaaa gtagctaagg aagaaaacaa aatcatgaaa aaaatgtgaa
      721 gtgatcatgc acatcgcatc aacttaaaca ttggcttaga gatatataga gttagagttt
      781 acggcaacct ttaagcacca ataccttttg gcatagtcta aagacctggt tcttaatttt
      841 aaacaaattt aactaaagat ttccctatca aagaagtaac gagttgacag attttctcaa
      901 aataaatcga tactgcattt ctaggcatat ccagcgagat ctatggtgtc caaaggagag
      961 gagttaatca aggaaaacat gagaatgaaa gttgtcatgg agggctccgt taatggtcac
     1021 caattcaagt gtacagggga aggtgaaggt aatccttaca tgggtacaca aactatgaga
     1081 attaaagtaa ttgaaggcgg accactacca tttgcatttg acattctggc aacgtcattc
     1141 atgtacggat cacgaacttt catcaagtac cctaaaggta taccagactt tttcaagcaa
     1201 tcttttccag agggttttac atgggaaagg gttacaagat acgaagatgg gggtgtcgtc
     1261 acagttatgc aagatacttc attagaagat ggctgccttg tctatcatgt gcaagtaaga
     1321 ggggtgaatt ttccttctaa cggacctgtg atgcagaaaa agaccaaagg ttgggaacca
     1381 aatactgaaa tgatgtaccc agctgatgga ggtttgagag gctacacaca catggcgctt
     1441 aaagttgatg gtggaggtca tttgtcttgt agttttgtta ccacttatcg ttctaaaaag
     1501 actgttggca atatcaaaat gccaggaata catgctgtag accacagact agaaagactc
     1561 gaagagagcg ataacgaaat gttcgttgta cagagagagc atgccgtagc caaatttgct
     1621 ggcttaggcg gtggtatgga tgaattgtat aagggatcct aactcgaggc gaatttctta
     1681 tgatttatga tttttattat taaataagtt ataaaaaaaa taagtgtata caaattttaa
     1741 agtgactctt aggttttaaa acgaaaattc ttattcttga gtaactcttt cctgtaggtc
     1801 aggttgcttt ctcaggtata gcatgaggtc gctcttattg accacacctc taccggcatg
     1861 ccgagcaaat gcctgcaaat cgctccccat ttcgctgagc atgagacgga aatctgctcg
     1921 tcagtggtgc tcacactgac gaatcatgta cagatcatac cgatgactgc ctggcgactc
     1981 acaactaagc aagacagccg gaaccagcgc cggcgaacac cactgcatat atggcatatc
     2041 acaacagtcc aactagtgca ctgcagtaca gcggccgcga ttatcaaaaa ggatcttcac
     2101 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     2161 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     2221 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     2281 accatctggc cccagtgctg caatgatacc gcgggaccca cgctcaccgg ctccagattt
     2341 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     2401 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     2461 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     2521 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     2581 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     2641 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     2701 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     2761 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     2821 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     2881 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     2941 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     3001 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     3061 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     3121 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgtcatga ccaaaatccc
     3181 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     3241 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     3301 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     3361 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     3421 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     3481 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     3541 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     3601 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     3661 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     3721 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     3781 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     3841 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcct
//
