LOCUS       sequence-259418-        3890 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pZF(3BS)-mRUBY2 cassette plasmid flanked by ConL2 and ConRE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   An-Adirekkun JM, Stewart CJ, Geller SH, Patel MT, Melendez J, Oakes 
            BL, Noyes MB, McClean MN
  TITLE     A yeast optogenetic toolkit (yOTK) for gene expression control in 
            Saccharomyces cerevisiae.
  JOURNAL   Biotechnol Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234.
  PUBMED    31788779
REFERENCE   2  (bases 1 to 3890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234."
COMMENT     Sequence Label: pMM0624
FEATURES             Location/Qualifiers
     source          1..3890
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     268..291
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             315..1025
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     terminator      1041..1228
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1096..1120)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(join(1545..2336,2337..2405))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2168..2187
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2406..2510)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2613..3201
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3102..3121
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tttatggtta tgaagaggaa aaattggcag taacctggcc ccacaaacct tcaaattaac
       61 gaatcaaatt aacaaccata ggatgataat gcgattagtt ttttagcctt atttctgggg
      121 taattaatca gcgaagcgat gatttttgat ctattaacag atatataaat ggaaaagctg
      181 cataaccact ttaactaata ctttcaacat tttcagtttg tattacttct tattcaaatg
      241 tcataaaagt atcaacaaaa aattgttaat atacctctat actttaacgt caaggagaaa
      301 aaactatagg tactatggtg tccaaaggag aggagttaat caaggaaaac atgagaatga
      361 aagttgtcat ggagggctcc gttaatggtc accaattcaa gtgtacaggg gaaggtgaag
      421 gtaatcctta catgggtaca caaactatga gaattaaagt aattgaaggc ggaccactac
      481 catttgcatt tgacattctg gcaacgtcat tcatgtacgg atcacgaact ttcatcaagt
      541 accctaaagg tataccagac tttttcaagc aatcttttcc agagggtttt acatgggaaa
      601 gggttacaag atacgaagat gggggtgtcg tcacagttat gcaagatact tcattagaag
      661 atggctgcct tgtctatcat gtgcaagtaa gaggggtgaa ttttccttct aacggacctg
      721 tgatgcagaa aaagaccaaa ggttgggaac caaatactga aatgatgtac ccagctgatg
      781 gaggtttgag aggctacaca cacatggcgc ttaaagttga tggtggaggt catttgtctt
      841 gtagttttgt taccacttat cgttctaaaa agactgttgg caatatcaaa atgccaggaa
      901 tacatgctgt agaccacaga ctagaaagac tcgaagagag cgataacgaa atgttcgttg
      961 tacagagaga gcatgccgta gccaaatttg ctggcttagg cggtggtatg gatgaattgt
     1021 ataagggatc ctaactcgag gcgaatttct tatgatttat gatttttatt attaaataag
     1081 ttataaaaaa aataagtgta tacaaatttt aaagtgactc ttaggtttta aaacgaaaat
     1141 tcttattctt gagtaactct ttcctgtagg tcaggttgct ttctcaggta tagcatgagg
     1201 tcgctcttat tgaccacacc tctaccggca tgccgagcaa atgcctgcaa atcgctcccc
     1261 atttcgctga gcatgagacg gaaatctgct cgtcagtggt gctcacactg acgaatcatg
     1321 tacagatcat accgatgact gcctggcgac tcacaactaa gcaagacagc cggaaccagc
     1381 gccggcgaac accactgcat atatggcata tcacaacagt ccaactagtg cactgcagta
     1441 cagcggccgc gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     1501 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     1561 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     1621 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     1681 ccgcgggacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     1741 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     1801 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     1861 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     1921 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     1981 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     2041 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     2101 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     2161 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     2221 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     2281 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     2341 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     2401 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     2461 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     2521 cgaaaagtgc cacctgtcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     2581 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     2641 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     2701 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     2761 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     2821 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     2881 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     2941 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     3001 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     3061 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     3121 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     3181 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     3241 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     3301 gcggccgccc ctgaattcgc atctagaata aatcgcagcc aagtgagtga atagatgacg
     3361 caccacggtc agacacggcc acatcgtatc tcacaggagc aagcgcgata ggagcactca
     3421 cacatagtac ggtgatccgc tgactccttt gcccaaataa gacgtgagcc cgtctcagat
     3481 gcacacactg gcttaagatg acaacgcgag gcaagctaaa cagatcttta tattgaattt
     3541 tcaaaaattc ttactttttt tttggatgga cgcaaagaag tttaataatc atattacatg
     3601 gcattaccac catatacata tccatataca tatccatatc taatcttact tatatgttgt
     3661 ggaaatgtaa agagccccat tatcttagcc taaaaaaacc ttctctttgg aactttcagt
     3721 aatacgctta actgctcatt gctatattga agtgcgcgcg cgcgtgggcg agcgtgggcg
     3781 agcgtgggcg tctagaccgt gcgtcctcgt cttcaccggt cgcgttcctg aaacgcagat
     3841 gtgcctcgcg ccgcactgct ccgaacaata aagattctac aatactagct
//
