LOCUS       sequence-25943-e         806 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral empty control vector, CMV/TO promoter, Neo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pLenti CMV/TO Neo empty (w215-1)
FEATURES             Location/Qualifiers
     source          1..806
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      35..171
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    211..332
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(222..241)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     276..295
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             404..637
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
ORIGIN
        1 cgactcacta tagggcgaat tgggtacgtc cctaggcctc caaaaaagcc tcctcacata
       61 cttctggaat agctcagagg ccgaggcggc ctcggcctct gcataaataa aaaaaattag
      121 tcagccatgg ggcggagaat gggcggaact gggcggagtt aggggcggga tgggcggagt
      181 taggggcggg atagctagag ccagacatga taagatacat tgatgagttt ggacaaacca
      241 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      301 ttgtaaccat tataagctgc aataaacaag ttcctctcac tctctgatat tcatttcttt
      361 gcaagttata aatactgaat aataagatga catgaactac tactgctaga gattttccac
      421 actgactaaa agggtctgag ggatctctag ttaccagagt cacacaacag acgggcacac
      481 actacttgaa gcactcaagg caagctttat tgaggcttaa gcagtgggtt ccctagttag
      541 ccagagagct cccaggctca gatctggtct aaccagagag acccagtaca agcaaaaagc
      601 agatcttgtc ttcgttggga gtgagctagc ccttccagtc cccccttttc ttttaaaaag
      661 tggctaagat ctacagctgc cttgtaagtc attggtctta aaggtaccgg gccctcgacg
      721 gatcccctca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag
      781 cggcgatacc gtaaagcacg aggaac
//
