LOCUS       sequence-259431-        2363 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Part plasmid for pZF(3BS), Type 2 (promoter) part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   An-Adirekkun JM, Stewart CJ, Geller SH, Patel MT, Melendez J, Oakes 
            BL, Noyes MB, McClean MN
  TITLE     A yeast optogenetic toolkit (yOTK) for gene expression control in 
            Saccharomyces cerevisiae.
  JOURNAL   Biotechnol Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234.
  PUBMED    31788779
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234."
COMMENT     Sequence Label: pMM0528
FEATURES             Location/Qualifiers
     source          1..2363
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(748..1336)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(828..847)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1518..2177
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1584..1603)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      2248..2282
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     primer_bind     complement(2322..2345)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
ORIGIN
        1 cttttatgac atttgaataa gaagtaatac aaactgaaaa tgttgaaagt attagttaaa
       61 gtggttatgc agcttttcca tttatatatc tgttaataga tcaaaaatca tcgcttcgct
      121 gattaattac cccagaaata aggctaaaaa actaatcgca ttatcatcct atggttgtta
      181 atttgattcg ttaatttgaa ggtttgtggg gccaggttac tgccaatttt tcctcttcat
      241 aaccataaaa gctagtattg tagaatcttt attgttcgga gcagtgcggc gcgaggcaca
      301 tctgcgtttc aggaacgcga ccggtgaaga cgaggacgca cggtctagac gcccacgctc
      361 gcccacgctc gcccacgcgc gcgcgcactt caatatagca atgagcagtt aagcgtatta
      421 ctgaaagttc caaagagaag gtttttttag gctaagataa tggggctctt tacatttcca
      481 caacatataa gtaagattag atatggatat gtatatggat atgtatatgg tggtaatgcc
      541 atgtaatatg attattaaac ttctttgcgt ccatccaaaa aaaaagtaag aatttttgaa
      601 aattcaatat aaagatctgt ttagcttgcc tcgcgtttga gaccgactac ggttatccac
      661 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
      721 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
      781 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
      841 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
      901 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
      961 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     1021 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     1081 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     1141 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     1201 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     1261 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     1321 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     1381 cgaaaactca cgttaaggga ttttggtcat gattgatcgg gcacgtaaga ggttccaact
     1441 ttcaccataa tgaaataaga tcactaccgg gcgtattttt tgagttatcg agattttcag
     1501 gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc
     1561 aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc
     1621 agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt
     1681 tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg gaatttcgta
     1741 tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt
     1801 tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac gatttccggc
     1861 agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc
     1921 ctaaagggtt tattgagaat atgtttttcg tttcagccaa tccctgggtg agtttcacca
     1981 gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc accatgggca
     2041 aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt catcatgccg
     2101 tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt
     2161 ggcagggcgg ggcgtaattt gatatcgagc tcgcttggac tcctgttgat agatccagta
     2221 atgacctcag aactccatct ggatttgttc agaacgctcg gttgccgccg ggcgtttttt
     2281 attggtctgg tctcacatag tacctatagt tttttctcct tgacgttaaa gtatagaggt
     2341 atattaacaa ttttttgttg ata
//
