LOCUS       sequence-259434-        3030 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Part plasmid for VP16-CIB1, Type 3 (coding sequence) part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   An-Adirekkun JM, Stewart CJ, Geller SH, Patel MT, Melendez J, Oakes 
            BL, Noyes MB, McClean MN
  TITLE     A yeast optogenetic toolkit (yOTK) for gene expression control in 
            Saccharomyces cerevisiae.
  JOURNAL   Biotechnol Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234.
  PUBMED    31788779
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234."
COMMENT     Sequence Label: pMM0531
FEATURES             Location/Qualifiers
     source          1..3030
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             111..131
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     359..378
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        359..377
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             402..911
                     /codon_start=1
                     /product="N-terminal portion of the CIB1 transcription 
                     factor from Arabidopsis thaliana"
                     /label=CIBN
                     /note="binds to CRY2 (cryptochrome 2) that has absorbed 
                     blue light (Kennedy et al., 2010)"
                     /translation="MNGAIGGDLLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSS
                     MITGGEMDSYLSTAGLNLPMMYGETTVEGDSRLSISPETTLGTGNFKKRKFDTETKDCN
                     EKKKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKE
                     LEKTDYI"
     terminator      complement(1465..1499)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1570..2229)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2144..2163
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2411..2999
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2900..2919
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
       61 ataaccgtag tcggtctcat atggataaag cggaattaat tcccgagcct ccaaaaaaga
      121 agagaaaggt cgaattgggt accgccgcct cggagctcca cttagacggc gaggacgtgg
      181 cgatggcgca tgccgacgcg ctagacgatt tcgatctgga catgttgggg gacggggatt
      241 ccccgggtcc gggatttacc ccccacgact ccgcccccta cggcgctctg gatatggccg
      301 acttcgagtt tgagcagatg tttaccgatg cccttggaat tgacgagtac ggtgggttta
      361 atacgactca ctatagggcg agcgccgaag ctagcgccac catgaatgga gctataggag
      421 gtgacctttt gctcaatttt cctgacatgt cggtcctaga gcgccaaagg gctcacctca
      481 agtacctcaa tcccaccttt gattctcctc tcgccggctt ctttgccgat tcttcaatga
      541 ttaccggcgg cgagatggac agctatcttt cgactgccgg tttgaatctt ccgatgatgt
      601 acggtgaaac gacggtggaa ggtgattcaa gactctcaat ttcgccggaa acgacgcttg
      661 ggactggaaa tttcaagaaa cggaagtttg atacagagac taaggattgt aatgagaaga
      721 agaagaagat gacgatgaac agagatgacc tagtagaaga aggagaagaa gagaagtcga
      781 aaataacaga gcaaaacaat gggagcacaa aaagcatcaa gaagatgaaa cacaaagcca
      841 agaaagaaga gaacaatttc tctaatgatt catctaaagt gacgaaggaa ttggagaaaa
      901 cggattatat tcatgttcgt gcacgacgag gccaagccac tgatagtcac agcatagcag
      961 aacgagttag aagagaaaag atcagtgaga gaatgaagtt tctacaagat ttggttcctg
     1021 gatgcgacaa gatcacaggc aaagcaggga tgcttgatga aatcattaac tatgttcagt
     1081 ctcttcagag acaaatcgag ttcttatcga tgaaactagc aattgtgaat ccaaggccgg
     1141 attttgatat ggatgacatt tttgccaaag aggttgcctc aactccaatg actgtggtgc
     1201 catctcctga aatggttctt tccggttatt ctcatgagat ggttcactct ggttattcta
     1261 gtgagatggt taactccggt taccttcatg tcaatccaat gcagcaagtg aataccagtt
     1321 ctgatccatt gtcatgcttc aacaatggcg aagctccttc gatgtgggac tctcatgtgc
     1381 agaatctcta tggcaattta ggagtaccgg tcatcgagct cgagctgcag atgaatcgta
     1441 gatacggatc ctgagaccag accaataaaa aacgcccggc ggcaaccgag cgttctgaac
     1501 aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc
     1561 gatatcaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat
     1621 tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag
     1681 caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc
     1741 catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg ctgaaacgaa
     1801 aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac
     1861 atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga
     1921 tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat
     1981 caccagctca ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc
     2041 aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa
     2101 ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc
     2161 ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt
     2221 tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg
     2281 tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgcccga tcaatcatga
     2341 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     2401 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     2461 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     2521 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     2581 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     2641 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     2701 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     2761 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     2821 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     2881 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     2941 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     3001 acgccagcaa cgcggccttt ttacggttcc
//
