LOCUS       sequence-259435-        3810 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Part plasmid for Zif268DBD-CRY2, Type 3 (coding sequence) part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   An-Adirekkun JM, Stewart CJ, Geller SH, Patel MT, Melendez J, Oakes 
            BL, Noyes MB, McClean MN
  TITLE     A yeast optogenetic toolkit (yOTK) for gene expression control in 
            Saccharomyces cerevisiae.
  JOURNAL   Biotechnol Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234.
  PUBMED    31788779
REFERENCE   2  (bases 1 to 3810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234."
COMMENT     Sequence Label: pMM0529
FEATURES             Location/Qualifiers
     source          1..3810
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..21
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             304..2139
                     /codon_start=1
                     /product="Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAAPDEIVADSFEALGANTIKE
                     PGLCPSVSSNDQQVPSAVRYNGSKRVKPEEEEERDMKKSRGFDERELFSTAESSSSSSV
                     FFVSQSCSLASEGKNLEGIQDSSDQITTSLGKNGCK"
     terminator      complement(2159..2193)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(2264..2923)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2838..2857
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      3105..3693
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3594..3613
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cccaagaaga agaggaaggt tggtattcac gggggtaccc gcccatatgc ttgccctgtc
       61 gagtcctgcg atcgccgctt ttctcgctcg gatgagctta cccgccatat ccgcatccat
      121 accggtcaga agcccttcca gtgtcgaatc tgcatgcgta acttcagtcg tagtgaccac
      181 cttaccaccc acatccgcac ccacacaggc gagaagcctt ttgcctgtga catttgtggg
      241 aggaagtttg ccaggagtga tgaacgcaag aggcatacca aaatccatac aggtgctagc
      301 ttcatgaaga tggacaaaaa gactatagtt tggtttagaa gagatctaag gattgaggat
      361 aatcctgcat tagcagcagc tgctcacgaa ggatctgttt ttcctgtctt catttggtgt
      421 cctgaagaag aaggacagtt ttatcctgga agagcttcaa gatggtggat gaaacaatca
      481 cttgctcact tatctcaatc cttgaaggct cttggatctg acctcacttt aatcaaaacc
      541 cacaacacga tttcagcgat cttggattgt atccgcgtta ccggtgctac aaaagtcgtc
      601 tttaaccacc tctatgatcc tgtttcgtta gttcgggacc ataccgtaaa ggagaagctg
      661 gtggaacgtg ggatctctgt gcaaagctac aatggagatc tattgtatga accgtgggag
      721 atatactgcg aaaagggcaa accttttacg agtttcaatt cttactggaa gaaatgctta
      781 gatatgtcga ttgaatccgt tatgcttcct cctccttggc ggttgatgcc aataactgca
      841 gcggctgaag cgatttgggc gtgttcgatt gaagaactag ggctggagaa tgaggccgag
      901 aaaccgagca atgcgttgtt aactagagct tggagtccag gatggagcaa tgctgataag
      961 ttactaaatg agttcatcga gaagcagttg atagattatg caaagaacag caagaaagtt
     1021 gttgggaatt ctacttcact actttctccg tatctccatt tcggggaaat aagcgtcaga
     1081 cacgttttcc agtgtgcccg gatgaaacaa attatatggg caagagataa gaacagtgaa
     1141 ggagaagaaa gtgcagatct ttttcttagg ggaatcggtt taagagagta ttctcggtat
     1201 atatgtttca acttcccgtt tactcacgag caatcgttgt tgagtcatct tcggtttttc
     1261 ccttgggatg ctgatgttga taagttcaag gcctggagac aaggcaggac cggttatccg
     1321 ttggtggatg ccggaatgag agagctttgg gctaccggat ggatgcataa cagaataaga
     1381 gtgattgttt caagctttgc tgtgaagttt cttctccttc catggaaatg gggaatgaag
     1441 tatttctggg atacactttt ggatgctgat ttggaatgtg acatccttgg ctggcagtat
     1501 atctctggga gtatccccga tggccacgag cttgatcgct tggacaatcc cgcgttacaa
     1561 ggcgccaaat atgacccaga aggtgagtac ataaggcaat ggcttcccga gcttgcgaga
     1621 ttgccaactg aatggatcca tcatccatgg gacgctcctt taaccgtact caaagcttct
     1681 ggtgtggaac tcggaacaaa ctatgcgaaa cccattgtag acatcgacac agctcgtgag
     1741 ctactagcta aagctatttc aagaacccgt gaagcacaga tcatgatcgg agcagcacct
     1801 gatgagattg tagcagatag cttcgaggcc ttaggggcta ataccattaa agaacctggt
     1861 ctttgcccat ctgtgtcttc taatgaccaa caagtacctt cggctgttcg ttacaacggg
     1921 tcaaagagag tgaaacctga ggaagaagaa gagagagaca tgaagaaatc taggggattc
     1981 gatgaaaggg agttgttttc gactgctgaa tcttcttctt cttcgagtgt gtttttcgtt
     2041 tcgcagtctt gctcgttggc atcagaaggg aagaatctgg aaggtattca agattcatct
     2101 gatcagatta ctacaagttt gggaaaaaat ggttgcaaag gatcctgaga ccagaccaat
     2161 aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat
     2221 tactggatct atcaacagga gtccaagcga gctcgatatc aaattacgcc ccgccctgcc
     2281 actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaaa
     2341 cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt
     2401 tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac
     2461 tggtgaaact cacccaggga ttggctgaaa cgaaaaacat attctcaata aaccctttag
     2521 ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact
     2581 gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga
     2641 aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca
     2701 tacgaaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa
     2761 acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct
     2821 ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt
     2881 gggatatatc aacggtggta tatccagtga tttttttctc cattttagct tccttagctc
     2941 ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa
     3001 agttggaacc tcttacgtgc ccgatcaatc atgaccaaaa tcccttaacg tgagttttcg
     3061 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     3121 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     3181 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     3241 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     3301 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     3361 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     3421 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     3481 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     3541 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     3601 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     3661 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     3721 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     3781 gtggataacc gtagtcggtc tcatatggcc
//
