LOCUS       sequence-259436-        3878 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pTDH3-mRUBY2 cassette plasmid flanked by ConL2 and ConRE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   An-Adirekkun JM, Stewart CJ, Geller SH, Patel MT, Melendez J, Oakes 
            BL, Noyes MB, McClean MN
  TITLE     A yeast optogenetic toolkit (yOTK) for gene expression control in 
            Saccharomyces cerevisiae.
  JOURNAL   Biotechnol Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234.
  PUBMED    31788779
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234."
COMMENT     Sequence Label: pMM0627
FEATURES             Location/Qualifiers
     source          1..3878
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..667
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     537..560
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     CDS             674..1384
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     terminator      1400..1587
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1455..1479)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(join(1904..2695,2696..2764))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2527..2546
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2765..2869)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2972..3560
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3461..3480
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcattatcaa tactgccatt tcaaagaata cgtaaataat taatagtagt gattttccta
       61 actttattta gtcaaaaaat tagcctttta attctgctgt aacccgtaca tgcccaaaat
      121 agggggcggg ttacacagaa tatataacat cgtaggtgtc tgggtgaaca gtttattcct
      181 ggcatccact aaatataatg gagcccgctt tttaagctgg catccagaaa aaaaaagaat
      241 cccagcacca aaatattgtt ttcttcacca accatcagtt cataggtcca ttctcttagc
      301 gcaactacag agaacagggg cacaaacagg caaaaaacgg gcacaacctc aatggagtga
      361 tgcaacctgc ctggagtaaa tgatgacaca aggcaattga cccacgcatg tatctatctc
      421 attttcttac accttctatt accttctgct ctctctgatt tggaaaaagc tgaaaaaaaa
      481 ggttgaaacc agttccctga aattattccc ctacttgact aataagtata taaagacggt
      541 aggtattgat tgtaattctg taaatctatt tcttaaactt cttaaattct acttttatag
      601 ttagtctttt ttttagtttt aaaacaccaa gaacttagtt tcgaataaac acacataaac
      661 aaacaaaaga tctatggtgt ccaaaggaga ggagttaatc aaggaaaaca tgagaatgaa
      721 agttgtcatg gagggctccg ttaatggtca ccaattcaag tgtacagggg aaggtgaagg
      781 taatccttac atgggtacac aaactatgag aattaaagta attgaaggcg gaccactacc
      841 atttgcattt gacattctgg caacgtcatt catgtacgga tcacgaactt tcatcaagta
      901 ccctaaaggt ataccagact ttttcaagca atcttttcca gagggtttta catgggaaag
      961 ggttacaaga tacgaagatg ggggtgtcgt cacagttatg caagatactt cattagaaga
     1021 tggctgcctt gtctatcatg tgcaagtaag aggggtgaat tttccttcta acggacctgt
     1081 gatgcagaaa aagaccaaag gttgggaacc aaatactgaa atgatgtacc cagctgatgg
     1141 aggtttgaga ggctacacac acatggcgct taaagttgat ggtggaggtc atttgtcttg
     1201 tagttttgtt accacttatc gttctaaaaa gactgttggc aatatcaaaa tgccaggaat
     1261 acatgctgta gaccacagac tagaaagact cgaagagagc gataacgaaa tgttcgttgt
     1321 acagagagag catgccgtag ccaaatttgc tggcttaggc ggtggtatgg atgaattgta
     1381 taagggatcc taactcgagg cgaatttctt atgatttatg atttttatta ttaaataagt
     1441 tataaaaaaa ataagtgtat acaaatttta aagtgactct taggttttaa aacgaaaatt
     1501 cttattcttg agtaactctt tcctgtaggt caggttgctt tctcaggtat agcatgaggt
     1561 cgctcttatt gaccacacct ctaccggcat gccgagcaaa tgcctgcaaa tcgctcccca
     1621 tttcgctgag catgagacgg aaatctgctc gtcagtggtg ctcacactga cgaatcatgt
     1681 acagatcata ccgatgactg cctggcgact cacaactaag caagacagcc ggaaccagcg
     1741 ccggcgaaca ccactgcata tatggcatat cacaacagtc caactagtgc actgcagtac
     1801 agcggccgcg attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     1861 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     1921 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     1981 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     2041 cgcgggaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     2101 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     2161 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     2221 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     2281 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     2341 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     2401 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     2461 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     2521 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     2581 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     2641 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     2701 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     2761 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     2821 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     2881 gaaaagtgcc acctgtcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     2941 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     3001 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     3061 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     3121 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     3181 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     3241 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     3301 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     3361 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     3421 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     3481 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     3541 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     3601 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgtg
     3661 cggccgcccc tgaattcgca tctagaataa atcgcagcca agtgagtgaa tagatgacgc
     3721 accacggtca gacacggcca catcgtatct cacaggagca agcgcgatag gagcactcac
     3781 acatagtacg gtgatccgct gactcctttg cccaaataag acgtgagccc gtctcagatg
     3841 cacacactgg cttaagatga caacgcagtt cgagttta
//
