LOCUS       sequence-259438-       10747 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Control for optogenetic machinery 
            (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pSTRONG-mRUBY2
            reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10747)
  AUTHORS   An-Adirekkun JM, Stewart CJ, Geller SH, Patel MT, Melendez J, Oakes 
            BL, Noyes MB, McClean MN
  TITLE     A yeast optogenetic toolkit (yOTK) for gene expression control in 
            Saccharomyces cerevisiae.
  JOURNAL   Biotechnol Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234.
  PUBMED    31788779
REFERENCE   2  (bases 1 to 10747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234."
COMMENT     Sequence Label: pMM0651
FEATURES             Location/Qualifiers
     source          1..10747
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             458..478
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     706..725
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        706..724
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             749..1258
                     /codon_start=1
                     /product="N-terminal portion of the CIB1 transcription 
                     factor from Arabidopsis thaliana"
                     /label=CIBN
                     /note="binds to CRY2 (cryptochrome 2) that has absorbed 
                     blue light (Kennedy et al., 2010)"
                     /translation="MNGAIGGDLLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSS
                     MITGGEMDSYLSTAGLNLPMMYGETTVEGDSRLSISPETTLGTGNFKKRKFDTETKDCN
                     EKKKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKE
                     LEKTDYI"
     CDS             2778..2798
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3081..4574
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     promoter        4867..5533
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     5403..5426
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     CDS             5540..6250
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     terminator      6266..6453
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(6321..6345)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             6716..7519
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     CDS             complement(8161..8976)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8161..8976)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8884..8903
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     8968..8987
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      9201..9789
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9690..9709
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(10742..10747,1..408)
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
ORIGIN
        1 ccacacacca tagcttcaaa atgtttctac tcctttttta ctcttccaga ttttctcgga
       61 ctccgcgcat cgccgtacca cttcaaaaca cccaagcaca gcatactaaa tttcccctct
      121 ttcttcctct agggtgtcgt taattacccg tactaaaggt ttggaaaaga aaaaagacac
      181 cgcctcgttt ctttttcttc gtcgaaaaag gcaataaaaa tttttatcac gtttcttttt
      241 cttgaaaatt tttttttttg atttttttct ctttcgatga cctcccattg atatttaagt
      301 taataaacgg tcatcaattt ctcaagtttc agtttcattt ttcttgttct attacaactt
      361 tttttacttc ttgctcatta gaaagaaagc atagcaatct aatctaagtt ttaattacaa
      421 aagatctatg gataaagcgg aattaattcc cgagcctcca aaaaagaaga gaaaggtcga
      481 attgggtacc gccgcctcgg agctccactt agacggcgag gacgtggcga tggcgcatgc
      541 cgacgcgcta gacgatttcg atctggacat gttgggggac ggggattccc cgggtccggg
      601 atttaccccc cacgactccg ccccctacgg cgctctggat atggccgact tcgagtttga
      661 gcagatgttt accgatgccc ttggaattga cgagtacggt gggtttaata cgactcacta
      721 tagggcgagc gccgaagcta gcgccaccat gaatggagct ataggaggtg accttttgct
      781 caattttcct gacatgtcgg tcctagagcg ccaaagggct cacctcaagt acctcaatcc
      841 cacctttgat tctcctctcg ccggcttctt tgccgattct tcaatgatta ccggcggcga
      901 gatggacagc tatctttcga ctgccggttt gaatcttccg atgatgtacg gtgaaacgac
      961 ggtggaaggt gattcaagac tctcaatttc gccggaaacg acgcttggga ctggaaattt
     1021 caagaaacgg aagtttgata cagagactaa ggattgtaat gagaagaaga agaagatgac
     1081 gatgaacaga gatgacctag tagaagaagg agaagaagag aagtcgaaaa taacagagca
     1141 aaacaatggg agcacaaaaa gcatcaagaa gatgaaacac aaagccaaga aagaagagaa
     1201 caatttctct aatgattcat ctaaagtgac gaaggaattg gagaaaacgg attatattca
     1261 tgttcgtgca cgacgaggcc aagccactga tagtcacagc atagcagaac gagttagaag
     1321 agaaaagatc agtgagagaa tgaagtttct acaagatttg gttcctggat gcgacaagat
     1381 cacaggcaaa gcagggatgc ttgatgaaat cattaactat gttcagtctc ttcagagaca
     1441 aatcgagttc ttatcgatga aactagcaat tgtgaatcca aggccggatt ttgatatgga
     1501 tgacattttt gccaaagagg ttgcctcaac tccaatgact gtggtgccat ctcctgaaat
     1561 ggttctttcc ggttattctc atgagatggt tcactctggt tattctagtg agatggttaa
     1621 ctccggttac cttcatgtca atccaatgca gcaagtgaat accagttctg atccattgtc
     1681 atgcttcaac aatggcgaag ctccttcgat gtgggactct catgtgcaga atctctatgg
     1741 caatttagga gtaccggtca tcgagctcga gctgcagatg aatcgtagat acggatccta
     1801 actcgagagc ttttgattaa gccttctagt ccaaaaaaca cgtttttttg tcatttattt
     1861 cattttctta gaatagttta gtttattcat tttatagtca cgaatgtttt atgattctat
     1921 atagggttgc aaacaagcat ttttcatttt atgttaaaac aatttcaggt ttacctttta
     1981 ttctgcttgt ggtgacgcgt gtatccgccc gctcttttgg tcacccatgt atgctgccaa
     2041 accagatgtc aacacagcta caacgaagag gatgtccaat atttttttta aggaataagg
     2101 atacttcaag actagattcc cccctgcatt cccatcagaa ccgtaaacct tggcgctttc
     2161 cttgggaagt attcaagaag tgccttgtcc ggtttctgtg gctcacaaac cagcgcgccc
     2221 gatatggctt tcttttcact tatgaatgta ccagtacggg acaattagaa cgctcctgta
     2281 acaatctctt tgcaaatgtg gggttacatt ctaaccatgt cacactgctg acgaaattca
     2341 aagtaaaaaa aaatgggacc acgtcttgag aacgatagat tttctttatt ttacattgaa
     2401 cagtcgttgt ctcagcgcgc tttatgtttt cattcatact tcatattata aaataacaaa
     2461 agaagaattt catattcacg cccaagaaat caggctgctt tccaaatgca attgacactt
     2521 cattagccat cacacaaaac tctttcttgc tggagcttct tttaaaaaag acctcagtac
     2581 accaaacacg ttacccgacc tcgttatttt acgacaacta tgataaaatt ctgaagaaaa
     2641 aataaaaaaa ttttcatact tcttgctttt atttaaacca ttgaatgatt tcttttgaac
     2701 aaaactacct gtttcaccaa aggaaataga aagaaaaaat caattagaag aaaacaaaaa
     2761 acaaaagatc tatggccccc aagaagaaga ggaaggttgg tattcacggg ggtacccgcc
     2821 catatgcttg ccctgtcgag tcctgcgatc gccgcttttc tcgctcggat gagcttaccc
     2881 gccatatccg catccatacc ggtcagaagc ccttccagtg tcgaatctgc atgcgtaact
     2941 tcagtcgtag tgaccacctt accacccaca tccgcaccca cacaggcgag aagccttttg
     3001 cctgtgacat ttgtgggagg aagtttgcca ggagtgatga acgcaagagg cataccaaaa
     3061 tccatacagg tgctagcttc atgaagatgg acaaaaagac tatagtttgg tttagaagag
     3121 atctaaggat tgaggataat cctgcattag cagcagctgc tcacgaagga tctgtttttc
     3181 ctgtcttcat ttggtgtcct gaagaagaag gacagtttta tcctggaaga gcttcaagat
     3241 ggtggatgaa acaatcactt gctcacttat ctcaatcctt gaaggctctt ggatctgacc
     3301 tcactttaat caaaacccac aacacgattt cagcgatctt ggattgtatc cgcgttaccg
     3361 gtgctacaaa agtcgtcttt aaccacctct atgatcctgt ttcgttagtt cgggaccata
     3421 ccgtaaagga gaagctggtg gaacgtggga tctctgtgca aagctacaat ggagatctat
     3481 tgtatgaacc gtgggagata tactgcgaaa agggcaaacc ttttacgagt ttcaattctt
     3541 actggaagaa atgcttagat atgtcgattg aatccgttat gcttcctcct ccttggcggt
     3601 tgatgccaat aactgcagcg gctgaagcga tttgggcgtg ttcgattgaa gaactagggc
     3661 tggagaatga ggccgagaaa ccgagcaatg cgttgttaac tagagcttgg agtccaggat
     3721 ggagcaatgc tgataagtta ctaaatgagt tcatcgagaa gcagttgata gattatgcaa
     3781 agaacagcaa gaaagttgtt gggaattcta cttcactact ttctccgtat ctccatttcg
     3841 gggaaataag cgtcagacac gttttccagt gtgcccggat gaaacaaatt atatgggcaa
     3901 gagataagaa cagtgaagga gaagaaagtg cagatctttt tcttagggga atcggtttaa
     3961 gagagtattc tcggtatata tgtttcaact tcccgtttac tcacgagcaa tcgttgttga
     4021 gtcatcttcg gtttttccct tgggatgctg atgttgataa gttcaaggcc tggagacaag
     4081 gcaggaccgg ttatccgttg gtggatgccg gaatgagaga gctttgggct accggatgga
     4141 tgcataacag aataagagtg attgtttcaa gctttgctgt gaagtttctt ctccttccat
     4201 ggaaatgggg aatgaagtat ttctgggata cacttttgga tgctgatttg gaatgtgaca
     4261 tccttggctg gcagtatatc tctgggagta tccccgatgg ccacgagctt gatcgcttgg
     4321 acaatcccgc gttacaaggc gccaaatatg acccagaagg tgagtacata aggcaatggc
     4381 ttcccgagct tgcgagattg ccaactgaat ggatccatca tccatgggac gctcctttaa
     4441 ccgtactcaa agcttctggt gtggaactcg gaacaaacta tgcgaaaccc attgtagaca
     4501 tcgacacagc tcgtgagcta ctagctaaag ctatttcaag aacccgtgaa gcacagatca
     4561 tgatcggagc agcagcccgg ggatcctaac tcgaggccaa ttggtgcggc aattgataat
     4621 aacgaaaatg tcttttaatg atctgggtat aatgaggaat tttccgaacg tttttacttt
     4681 atatatatat atacatgtaa catatattct atacgctata gagaaaggaa atttttcaat
     4741 taaaaaaaaa atagagaaag agtttcactt cttgattatc gctaacacta atggttgaag
     4801 tactgctact ttaattttat gctggatgca cacactggct taagatgaca acgcagttcg
     4861 agtttatcat tatcaatact gccatttcaa agaatacgta aataattaat agtagtgatt
     4921 ttcctaactt tatttagtca aaaaattagc cttttaattc tgctgtaacc cgtacatgcc
     4981 caaaataggg ggcgggttac acagaatata taacatcgta ggtgtctggg tgaacagttt
     5041 attcctggca tccactaaat ataatggagc ccgcttttta agctggcatc cagaaaaaaa
     5101 aagaatccca gcaccaaaat attgttttct tcaccaacca tcagttcata ggtccattct
     5161 cttagcgcaa ctacagagaa caggggcaca aacaggcaaa aaacgggcac aacctcaatg
     5221 gagtgatgca acctgcctgg agtaaatgat gacacaaggc aattgaccca cgcatgtatc
     5281 tatctcattt tcttacacct tctattacct tctgctctct ctgatttgga aaaagctgaa
     5341 aaaaaaggtt gaaaccagtt ccctgaaatt attcccctac ttgactaata agtatataaa
     5401 gacggtaggt attgattgta attctgtaaa tctatttctt aaacttctta aattctactt
     5461 ttatagttag tctttttttt agttttaaaa caccaagaac ttagtttcga ataaacacac
     5521 ataaacaaac aaaagatcta tggtgtccaa aggagaggag ttaatcaagg aaaacatgag
     5581 aatgaaagtt gtcatggagg gctccgttaa tggtcaccaa ttcaagtgta caggggaagg
     5641 tgaaggtaat ccttacatgg gtacacaaac tatgagaatt aaagtaattg aaggcggacc
     5701 actaccattt gcatttgaca ttctggcaac gtcattcatg tacggatcac gaactttcat
     5761 caagtaccct aaaggtatac cagacttttt caagcaatct tttccagagg gttttacatg
     5821 ggaaagggtt acaagatacg aagatggggg tgtcgtcaca gttatgcaag atacttcatt
     5881 agaagatggc tgccttgtct atcatgtgca agtaagaggg gtgaattttc cttctaacgg
     5941 acctgtgatg cagaaaaaga ccaaaggttg ggaaccaaat actgaaatga tgtacccagc
     6001 tgatggaggt ttgagaggct acacacacat ggcgcttaaa gttgatggtg gaggtcattt
     6061 gtcttgtagt tttgttacca cttatcgttc taaaaagact gttggcaata tcaaaatgcc
     6121 aggaatacat gctgtagacc acagactaga aagactcgaa gagagcgata acgaaatgtt
     6181 cgttgtacag agagagcatg ccgtagccaa atttgctggc ttaggcggtg gtatggatga
     6241 attgtataag ggatcctaac tcgaggcgaa tttcttatga tttatgattt ttattattaa
     6301 ataagttata aaaaaaataa gtgtatacaa attttaaagt gactcttagg ttttaaaacg
     6361 aaaattctta ttcttgagta actctttcct gtaggtcagg ttgctttctc aggtatagca
     6421 tgaggtcgct cttattgacc acacctctac cggcatgccg agcaaatgcc tgcaaatcgc
     6481 tccccatttc gctgagcagt tacagagatg ttacgaacca ctagtgcact gcagtacacg
     6541 gtttccttga aatttttttg attcggtaat ctccgaacag aaggaagaac gaaggaagga
     6601 gcacagactt agattggtat atatacgcat atgtagtgtt gaagaaacat gaaattgccc
     6661 agtattctta acccaactgc acagaacaaa aacgtgcagg aaacgaagat aaatcatgtc
     6721 gaaagctaca tataaggaac gtgctgctac tcatcctagt cctgttgctg ccaagctatt
     6781 taatatcatg cacgaaaagc aaacaaactt gtgtgcttca ttggatgttc gtaccaccaa
     6841 ggaattactg gagttagttg aagcattagg tcccaaaatt tgtttactaa aaacacatgt
     6901 ggatatcttg actgattttt ccatggaggg cacagttaag ccgctaaagg cattatccgc
     6961 caagtacaat tttttactct tcgaggacag aaaatttgct gacattggta atacagtcaa
     7021 attgcagtac tctgcgggtg tatacagaat agcagaatgg gcagacatta cgaatgcaca
     7081 cggtgtggtg ggcccaggta ttgttagcgg tttgaagcag gcggcagaag aagtaacaaa
     7141 ggaacctaga ggccttttga tgttagcaga attgtcatgc aagggctccc tatctactgg
     7201 agaatatact aagggtactg ttgacattgc gaagagcgac aaagattttg ttatcggctt
     7261 tattgctcaa agagacatgg gtggaagaga tgaaggttac gattggttga ttatgacacc
     7321 cggtgtgggt ttagatgaca agggagatgc attgggtcaa cagtatagaa ccgtggatga
     7381 tgtggtttct acaggatctg acattattat tgttggaaga ggactatttg caaagggaag
     7441 ggatgctaag gtagagggtg aacgttacag aaaagcaggc tgggaagcat atttgagaag
     7501 atgcggccag caaaactaaa aaactgtatt ataagtaaat gcatgtatac taaactcaca
     7561 aattagagct tcaatttaat tatatcagtt attacccgag tagagcactt gaatccactg
     7621 ccccgggaat ctcggtcgta atgatttcta taatgacgaa aaaaaaaaaa ttggaaagaa
     7681 aaagcttcat ggcctttata aaaaggaact atccaatacc tcgccagaac caagtaacag
     7741 tattttacgg ggcacaaatc aagaacaata agacaggact gtaaagatgg acgcattgaa
     7801 ctccaaagaa caacaagagt tccaaaaagt agtggaacaa aagcaaatga aggatttcat
     7861 gcgtttgtac tctaatctgg tagaaagatg tttcacagac tgtgtcaatg acttcacaac
     7921 atcaaagcta accaataagg aacaaacatg catcatgaag tgctcagaaa agttcttgaa
     7981 gcatagcgaa cgtgtagggc agcgtttcca agaacaaaac gctgccttgg gacaaggctt
     8041 gggccgataa ggtgtactgg cgtatatata tctaattatg tatctctggt gtagcccatt
     8101 tttagcatgt aaatataaag accgagcggc cgcgtgttac aaccaattaa ccaattctga
     8161 ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag gattatcaat
     8221 accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga ggcagttcca
     8281 taggatggca agatcctggt atcggtctgc gattccgact cgtccaacat caatacaacc
     8341 tattaatttc ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat gagtgacgac
     8401 tgaatccggt gagaatggca aaagcttatg catttctttc cagacttgtt caacaggcca
     8461 gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca ttcgtgattg
     8521 cgcctgagcg aggcgaaata cgcgatcgct gttaaaagga caattacaaa caggaatcga
     8581 atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata ttttcacctg aatcaggata
     8641 ttcttctaat acctggaatg ctgttttccc ggggatcgca gtggtgagta accatgcatc
     8701 atcaggagta cggataaaat gcttgatggt cggaagaggc ataaattccg tcagccagtt
     8761 tagtctgacc atctcatctg taacatcatt ggcaacgcta cctttgccat gtttcagaaa
     8821 caactctggc gcatcgggct tcccatacaa tcgatagatt gtcgcacctg attgcccgac
     8881 attatcgcga gcccatttat acccatataa atcagcatcc atgttggaat ttaatcgcgg
     8941 cctggagcaa gacgtttccc gttgaatatg gctcataaca ccccttgtat tactgtttat
     9001 gtaagcagac agttttattg ttcatgatga tatattttta tcttgtgcaa tgtaacatca
     9061 gagattttga gacacaacgt ggctttgttg aataaatcga acttttgctg agttgaagga
     9121 tcagtcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     9181 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     9241 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     9301 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     9361 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     9421 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     9481 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     9541 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     9601 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     9661 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     9721 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     9781 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     9841 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtgc ggccgccaat
     9901 gctaaattcg agtgaaacac aggaagatca gaaaatcctc atttcatcca tattaacaat
     9961 aatttcaaat gtttatttgc attatttgaa actaggcaag acaagcaacg aaacgttttt
    10021 gaaaattttg agtattttca ataaatttgt agaggactca gatattgaaa aaaagctaca
    10081 gcaattaata cttgataaga agagtattga gaagggcaac ggttcatcat ctcatggatc
    10141 tgcacatgaa caaacaccag agtcaaacga cgttgaaatt gaggctactg cgccaattga
    10201 tgacaataca gacgatgata acaaaccgaa gttatctgat gtagaaaagg attaaagatg
    10261 ctaagagata gtgatgatat ttcataaata atgtaattct atatatgtta attacctttt
    10321 ttgcgaggca tatttatggt gaaggataag ttttgaccat caaagaaggt taatgtggct
    10381 gtggtttcag ggtccataaa gcccacatgg ataacattac ccctgaattc gcatctagac
    10441 tgaactggcc gataattgca gacgaacgcc ttgccaacag ggagttcttc agagacatgg
    10501 aggctcaaaa cgaaattatt gacagcctag acatcaatag tcatacaaca gaaagcgacc
    10561 acccaacttt ggctgataat agcgtataaa caatgcatac tttgtacgtt caaaatacaa
    10621 tgcagtagat atatttatgc atattacata taatacatat cacataggaa gcaacaggcg
    10681 cgttggactt ttaattttcg aggaccgcga atccttacat cacacccaat cccccacaag
    10741 tgatccc
//
