LOCUS       sequence-259439-        4996 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pRPL18B-Zif268DBD-CRY2PHR cassette plasmid flanked by ConL1 and 
            ConR2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4996)
  AUTHORS   An-Adirekkun JM, Stewart CJ, Geller SH, Patel MT, Melendez J, Oakes 
            BL, Noyes MB, McClean MN
  TITLE     A yeast optogenetic toolkit (yOTK) for gene expression control in 
            Saccharomyces cerevisiae.
  JOURNAL   Biotechnol Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234.
  PUBMED    31788779
REFERENCE   2  (bases 1 to 4996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234."
COMMENT     Sequence Label: pMM0623
FEATURES             Location/Qualifiers
     source          1..4996
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             341..361
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             644..2137
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     CDS             complement(join(2663..3454,3455..3523))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3286..3305
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3524..3628)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3731..4319
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4220..4239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acttcatatt ataaaataac aaaagaagaa tttcatattc acgcccaaga aatcaggctg
       61 ctttccaaat gcaattgaca cttcattagc catcacacaa aactctttct tgctggagct
      121 tcttttaaaa aagacctcag tacaccaaac acgttacccg acctcgttat tttacgacaa
      181 ctatgataaa attctgaaga aaaaataaaa aaattttcat acttcttgct tttatttaaa
      241 ccattgaatg atttcttttg aacaaaacta cctgtttcac caaaggaaat agaaagaaaa
      301 aatcaattag aagaaaacaa aaaacaaaag atctatggcc cccaagaaga agaggaaggt
      361 tggtattcac gggggtaccc gcccatatgc ttgccctgtc gagtcctgcg atcgccgctt
      421 ttctcgctcg gatgagctta cccgccatat ccgcatccat accggtcaga agcccttcca
      481 gtgtcgaatc tgcatgcgta acttcagtcg tagtgaccac cttaccaccc acatccgcac
      541 ccacacaggc gagaagcctt ttgcctgtga catttgtggg aggaagtttg ccaggagtga
      601 tgaacgcaag aggcatacca aaatccatac aggtgctagc ttcatgaaga tggacaaaaa
      661 gactatagtt tggtttagaa gagatctaag gattgaggat aatcctgcat tagcagcagc
      721 tgctcacgaa ggatctgttt ttcctgtctt catttggtgt cctgaagaag aaggacagtt
      781 ttatcctgga agagcttcaa gatggtggat gaaacaatca cttgctcact tatctcaatc
      841 cttgaaggct cttggatctg acctcacttt aatcaaaacc cacaacacga tttcagcgat
      901 cttggattgt atccgcgtta ccggtgctac aaaagtcgtc tttaaccacc tctatgatcc
      961 tgtttcgtta gttcgggacc ataccgtaaa ggagaagctg gtggaacgtg ggatctctgt
     1021 gcaaagctac aatggagatc tattgtatga accgtgggag atatactgcg aaaagggcaa
     1081 accttttacg agtttcaatt cttactggaa gaaatgctta gatatgtcga ttgaatccgt
     1141 tatgcttcct cctccttggc ggttgatgcc aataactgca gcggctgaag cgatttgggc
     1201 gtgttcgatt gaagaactag ggctggagaa tgaggccgag aaaccgagca atgcgttgtt
     1261 aactagagct tggagtccag gatggagcaa tgctgataag ttactaaatg agttcatcga
     1321 gaagcagttg atagattatg caaagaacag caagaaagtt gttgggaatt ctacttcact
     1381 actttctccg tatctccatt tcggggaaat aagcgtcaga cacgttttcc agtgtgcccg
     1441 gatgaaacaa attatatggg caagagataa gaacagtgaa ggagaagaaa gtgcagatct
     1501 ttttcttagg ggaatcggtt taagagagta ttctcggtat atatgtttca acttcccgtt
     1561 tactcacgag caatcgttgt tgagtcatct tcggtttttc ccttgggatg ctgatgttga
     1621 taagttcaag gcctggagac aaggcaggac cggttatccg ttggtggatg ccggaatgag
     1681 agagctttgg gctaccggat ggatgcataa cagaataaga gtgattgttt caagctttgc
     1741 tgtgaagttt cttctccttc catggaaatg gggaatgaag tatttctggg atacactttt
     1801 ggatgctgat ttggaatgtg acatccttgg ctggcagtat atctctggga gtatccccga
     1861 tggccacgag cttgatcgct tggacaatcc cgcgttacaa ggcgccaaat atgacccaga
     1921 aggtgagtac ataaggcaat ggcttcccga gcttgcgaga ttgccaactg aatggatcca
     1981 tcatccatgg gacgctcctt taaccgtact caaagcttct ggtgtggaac tcggaacaaa
     2041 ctatgcgaaa cccattgtag acatcgacac agctcgtgag ctactagcta aagctatttc
     2101 aagaacccgt gaagcacaga tcatgatcgg agcagcagcc cggggatcct aactcgaggc
     2161 caattggtgc ggcaattgat aataacgaaa atgtctttta atgatctggg tataatgagg
     2221 aattttccga acgtttttac tttatatata tatatacatg taacatatat tctatacgct
     2281 atagagaaag gaaatttttc aattaaaaaa aaaatagaga aagagtttca cttcttgatt
     2341 atcgctaaca ctaatggttg aagtactgct actttaattt tatgctggat gtgagacgat
     2401 aaatcgcagc caagtgagtg aatagatgac gcaccacggt cagacacggc cacatcgtat
     2461 ctcacaggag caagcgcgat aggagcactc acacatagta cggtgatccg ctgactcctt
     2521 tgcccaaata agacgtgagc cactagtgca ctgcagtaca gcggccgcga ttatcaaaaa
     2581 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     2641 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     2701 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     2761 gggagggctt accatctggc cccagtgctg caatgatacc gcgggaccca cgctcaccgg
     2821 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     2881 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     2941 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     3001 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     3061 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     3121 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     3181 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     3241 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     3301 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     3361 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     3421 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     3481 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     3541 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     3601 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgtcatga
     3661 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     3721 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     3781 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     3841 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     3901 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     3961 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     4021 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     4081 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     4141 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     4201 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     4261 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     4321 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     4381 tctttcctgc gttatcccct gattctgtgg ataaccgtgc ggccgcccct gaattcgcat
     4441 ctagaacggg gtcatcacgg ctcatcatgc gccaaacaaa tgtgtgcaat acacgctcgg
     4501 atgactgcat gatgaccgca ctgactgggg acagcagatc cacctaagcc tgtgagagaa
     4561 gcagacaccc gacagatcaa ggcagttacg tctcaccaaa ccagatgtca acacagctac
     4621 aacgaagagg atgtccaata ttttttttaa ggaataagga tacttcaaga ctagattccc
     4681 ccctgcattc ccatcagaac cgtaaacctt ggcgctttcc ttgggaagta ttcaagaagt
     4741 gccttgtccg gtttctgtgg ctcacaaacc agcgcgcccg atatggcttt cttttcactt
     4801 atgaatgtac cagtacggga caattagaac gctcctgtaa caatctcttt gcaaatgtgg
     4861 ggttacattc taaccatgtc acactgctga cgaaattcaa agtaaaaaaa aatgggacca
     4921 cgtcttgaga acgatagatt ttctttattt tacattgaac agtcgttgtc tcagcgcgct
     4981 ttatgttttc attcat
//
