LOCUS       sequence-259441-       11259 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integration vector for optogenetic machinery 
            (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2 with loxable 
            KIURA3 marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11259)
  AUTHORS   An-Adirekkun JM, Stewart CJ, Geller SH, Patel MT, Melendez J, Oakes 
            BL, Noyes MB, McClean MN
  TITLE     A yeast optogenetic toolkit (yOTK) for gene expression control in 
            Saccharomyces cerevisiae.
  JOURNAL   Biotechnol Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234.
  PUBMED    31788779
REFERENCE   2  (bases 1 to 11259)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234."
COMMENT     Sequence Label: pMM0646
FEATURES             Location/Qualifiers
     source          1..11259
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             458..478
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     706..725
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        706..724
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             749..1258
                     /codon_start=1
                     /product="N-terminal portion of the CIB1 transcription 
                     factor from Arabidopsis thaliana"
                     /label=CIBN
                     /note="binds to CRY2 (cryptochrome 2) that has absorbed 
                     blue light (Kennedy et al., 2010)"
                     /translation="MNGAIGGDLLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSS
                     MITGGEMDSYLSTAGLNLPMMYGETTVEGDSRLSISPETTLGTGNFKKRKFDTETKDCN
                     EKKKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKE
                     LEKTDYI"
     CDS             2778..2798
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3081..4574
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     primer_bind     5505..5528
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             5552..6262
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     terminator      6278..6465
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(6333..6357)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     protein_bind    6571..6604
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    8056..8089
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(8673..9488)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8673..9488)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9396..9415
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     9480..9499
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      9713..10301
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10202..10221
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(11254..11259,1..408)
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
ORIGIN
        1 ccacacacca tagcttcaaa atgtttctac tcctttttta ctcttccaga ttttctcgga
       61 ctccgcgcat cgccgtacca cttcaaaaca cccaagcaca gcatactaaa tttcccctct
      121 ttcttcctct agggtgtcgt taattacccg tactaaaggt ttggaaaaga aaaaagacac
      181 cgcctcgttt ctttttcttc gtcgaaaaag gcaataaaaa tttttatcac gtttcttttt
      241 cttgaaaatt tttttttttg atttttttct ctttcgatga cctcccattg atatttaagt
      301 taataaacgg tcatcaattt ctcaagtttc agtttcattt ttcttgttct attacaactt
      361 tttttacttc ttgctcatta gaaagaaagc atagcaatct aatctaagtt ttaattacaa
      421 aagatctatg gataaagcgg aattaattcc cgagcctcca aaaaagaaga gaaaggtcga
      481 attgggtacc gccgcctcgg agctccactt agacggcgag gacgtggcga tggcgcatgc
      541 cgacgcgcta gacgatttcg atctggacat gttgggggac ggggattccc cgggtccggg
      601 atttaccccc cacgactccg ccccctacgg cgctctggat atggccgact tcgagtttga
      661 gcagatgttt accgatgccc ttggaattga cgagtacggt gggtttaata cgactcacta
      721 tagggcgagc gccgaagcta gcgccaccat gaatggagct ataggaggtg accttttgct
      781 caattttcct gacatgtcgg tcctagagcg ccaaagggct cacctcaagt acctcaatcc
      841 cacctttgat tctcctctcg ccggcttctt tgccgattct tcaatgatta ccggcggcga
      901 gatggacagc tatctttcga ctgccggttt gaatcttccg atgatgtacg gtgaaacgac
      961 ggtggaaggt gattcaagac tctcaatttc gccggaaacg acgcttggga ctggaaattt
     1021 caagaaacgg aagtttgata cagagactaa ggattgtaat gagaagaaga agaagatgac
     1081 gatgaacaga gatgacctag tagaagaagg agaagaagag aagtcgaaaa taacagagca
     1141 aaacaatggg agcacaaaaa gcatcaagaa gatgaaacac aaagccaaga aagaagagaa
     1201 caatttctct aatgattcat ctaaagtgac gaaggaattg gagaaaacgg attatattca
     1261 tgttcgtgca cgacgaggcc aagccactga tagtcacagc atagcagaac gagttagaag
     1321 agaaaagatc agtgagagaa tgaagtttct acaagatttg gttcctggat gcgacaagat
     1381 cacaggcaaa gcagggatgc ttgatgaaat cattaactat gttcagtctc ttcagagaca
     1441 aatcgagttc ttatcgatga aactagcaat tgtgaatcca aggccggatt ttgatatgga
     1501 tgacattttt gccaaagagg ttgcctcaac tccaatgact gtggtgccat ctcctgaaat
     1561 ggttctttcc ggttattctc atgagatggt tcactctggt tattctagtg agatggttaa
     1621 ctccggttac cttcatgtca atccaatgca gcaagtgaat accagttctg atccattgtc
     1681 atgcttcaac aatggcgaag ctccttcgat gtgggactct catgtgcaga atctctatgg
     1741 caatttagga gtaccggtca tcgagctcga gctgcagatg aatcgtagat acggatccta
     1801 actcgagagc ttttgattaa gccttctagt ccaaaaaaca cgtttttttg tcatttattt
     1861 cattttctta gaatagttta gtttattcat tttatagtca cgaatgtttt atgattctat
     1921 atagggttgc aaacaagcat ttttcatttt atgttaaaac aatttcaggt ttacctttta
     1981 ttctgcttgt ggtgacgcgt gtatccgccc gctcttttgg tcacccatgt atgctgccaa
     2041 accagatgtc aacacagcta caacgaagag gatgtccaat atttttttta aggaataagg
     2101 atacttcaag actagattcc cccctgcatt cccatcagaa ccgtaaacct tggcgctttc
     2161 cttgggaagt attcaagaag tgccttgtcc ggtttctgtg gctcacaaac cagcgcgccc
     2221 gatatggctt tcttttcact tatgaatgta ccagtacggg acaattagaa cgctcctgta
     2281 acaatctctt tgcaaatgtg gggttacatt ctaaccatgt cacactgctg acgaaattca
     2341 aagtaaaaaa aaatgggacc acgtcttgag aacgatagat tttctttatt ttacattgaa
     2401 cagtcgttgt ctcagcgcgc tttatgtttt cattcatact tcatattata aaataacaaa
     2461 agaagaattt catattcacg cccaagaaat caggctgctt tccaaatgca attgacactt
     2521 cattagccat cacacaaaac tctttcttgc tggagcttct tttaaaaaag acctcagtac
     2581 accaaacacg ttacccgacc tcgttatttt acgacaacta tgataaaatt ctgaagaaaa
     2641 aataaaaaaa ttttcatact tcttgctttt atttaaacca ttgaatgatt tcttttgaac
     2701 aaaactacct gtttcaccaa aggaaataga aagaaaaaat caattagaag aaaacaaaaa
     2761 acaaaagatc tatggccccc aagaagaaga ggaaggttgg tattcacggg ggtacccgcc
     2821 catatgcttg ccctgtcgag tcctgcgatc gccgcttttc tcgctcggat gagcttaccc
     2881 gccatatccg catccatacc ggtcagaagc ccttccagtg tcgaatctgc atgcgtaact
     2941 tcagtcgtag tgaccacctt accacccaca tccgcaccca cacaggcgag aagccttttg
     3001 cctgtgacat ttgtgggagg aagtttgcca ggagtgatga acgcaagagg cataccaaaa
     3061 tccatacagg tgctagcttc atgaagatgg acaaaaagac tatagtttgg tttagaagag
     3121 atctaaggat tgaggataat cctgcattag cagcagctgc tcacgaagga tctgtttttc
     3181 ctgtcttcat ttggtgtcct gaagaagaag gacagtttta tcctggaaga gcttcaagat
     3241 ggtggatgaa acaatcactt gctcacttat ctcaatcctt gaaggctctt ggatctgacc
     3301 tcactttaat caaaacccac aacacgattt cagcgatctt ggattgtatc cgcgttaccg
     3361 gtgctacaaa agtcgtcttt aaccacctct atgatcctgt ttcgttagtt cgggaccata
     3421 ccgtaaagga gaagctggtg gaacgtggga tctctgtgca aagctacaat ggagatctat
     3481 tgtatgaacc gtgggagata tactgcgaaa agggcaaacc ttttacgagt ttcaattctt
     3541 actggaagaa atgcttagat atgtcgattg aatccgttat gcttcctcct ccttggcggt
     3601 tgatgccaat aactgcagcg gctgaagcga tttgggcgtg ttcgattgaa gaactagggc
     3661 tggagaatga ggccgagaaa ccgagcaatg cgttgttaac tagagcttgg agtccaggat
     3721 ggagcaatgc tgataagtta ctaaatgagt tcatcgagaa gcagttgata gattatgcaa
     3781 agaacagcaa gaaagttgtt gggaattcta cttcactact ttctccgtat ctccatttcg
     3841 gggaaataag cgtcagacac gttttccagt gtgcccggat gaaacaaatt atatgggcaa
     3901 gagataagaa cagtgaagga gaagaaagtg cagatctttt tcttagggga atcggtttaa
     3961 gagagtattc tcggtatata tgtttcaact tcccgtttac tcacgagcaa tcgttgttga
     4021 gtcatcttcg gtttttccct tgggatgctg atgttgataa gttcaaggcc tggagacaag
     4081 gcaggaccgg ttatccgttg gtggatgccg gaatgagaga gctttgggct accggatgga
     4141 tgcataacag aataagagtg attgtttcaa gctttgctgt gaagtttctt ctccttccat
     4201 ggaaatgggg aatgaagtat ttctgggata cacttttgga tgctgatttg gaatgtgaca
     4261 tccttggctg gcagtatatc tctgggagta tccccgatgg ccacgagctt gatcgcttgg
     4321 acaatcccgc gttacaaggc gccaaatatg acccagaagg tgagtacata aggcaatggc
     4381 ttcccgagct tgcgagattg ccaactgaat ggatccatca tccatgggac gctcctttaa
     4441 ccgtactcaa agcttctggt gtggaactcg gaacaaacta tgcgaaaccc attgtagaca
     4501 tcgacacagc tcgtgagcta ctagctaaag ctatttcaag aacccgtgaa gcacagatca
     4561 tgatcggagc agcagcccgg ggatcctaac tcgaggccaa ttggtgcggc aattgataat
     4621 aacgaaaatg tcttttaatg atctgggtat aatgaggaat tttccgaacg tttttacttt
     4681 atatatatat atacatgtaa catatattct atacgctata gagaaaggaa atttttcaat
     4741 taaaaaaaaa atagagaaag agtttcactt cttgattatc gctaacacta atggttgaag
     4801 tactgctact ttaattttat gctggatgca cacactggct taagatgaca acgcgaggca
     4861 agctaaacag atctttatat tgaattttca aaaattctta cttttttttt ggatggacgc
     4921 aaagaagttt aataatcata ttacatggca ttaccaccat atacatatcc atatacatat
     4981 ccatatctaa tcttacttat atgttgtgga aatgtaaaga gccccattat cttagcctaa
     5041 aaaaaccttc tctttggaac tttcagtaat acgcttaact gctcattgct atattgaagt
     5101 gcgcgcgcgc gtgggcgagc gtgggcgagc gtgggcgtct agaccgtgcg tcctcgtctt
     5161 caccggtcgc gttcctgaaa cgcagatgtg cctcgcgccg cactgctccg aacaataaag
     5221 attctacaat actagctttt atggttatga agaggaaaaa ttggcagtaa cctggcccca
     5281 caaaccttca aattaacgaa tcaaattaac aaccatagga tgataatgcg attagttttt
     5341 tagccttatt tctggggtaa ttaatcagcg aagcgatgat ttttgatcta ttaacagata
     5401 tataaatgga aaagctgcat aaccacttta actaatactt tcaacatttt cagtttgtat
     5461 tacttcttat tcaaatgtca taaaagtatc aacaaaaaat tgttaatata cctctatact
     5521 ttaacgtcaa ggagaaaaaa ctataggtac tatggtgtcc aaaggagagg agttaatcaa
     5581 ggaaaacatg agaatgaaag ttgtcatgga gggctccgtt aatggtcacc aattcaagtg
     5641 tacaggggaa ggtgaaggta atccttacat gggtacacaa actatgagaa ttaaagtaat
     5701 tgaaggcgga ccactaccat ttgcatttga cattctggca acgtcattca tgtacggatc
     5761 acgaactttc atcaagtacc ctaaaggtat accagacttt ttcaagcaat cttttccaga
     5821 gggttttaca tgggaaaggg ttacaagata cgaagatggg ggtgtcgtca cagttatgca
     5881 agatacttca ttagaagatg gctgccttgt ctatcatgtg caagtaagag gggtgaattt
     5941 tccttctaac ggacctgtga tgcagaaaaa gaccaaaggt tgggaaccaa atactgaaat
     6001 gatgtaccca gctgatggag gtttgagagg ctacacacac atggcgctta aagttgatgg
     6061 tggaggtcat ttgtcttgta gttttgttac cacttatcgt tctaaaaaga ctgttggcaa
     6121 tatcaaaatg ccaggaatac atgctgtaga ccacagacta gaaagactcg aagagagcga
     6181 taacgaaatg ttcgttgtac agagagagca tgccgtagcc aaatttgctg gcttaggcgg
     6241 tggtatggat gaattgtata agggatccta actcgaggcg aatttcttat gatttatgat
     6301 ttttattatt aaataagtta taaaaaaaat aagtgtatac aaattttaaa gtgactctta
     6361 ggttttaaaa cgaaaattct tattcttgag taactctttc ctgtaggtca ggttgctttc
     6421 tcaggtatag catgaggtcg ctcttattga ccacacctct accggcatgc cgagcaaatg
     6481 cctgcaaatc gctccccatt tcgctgagca gttacagaga tgttacgaac cactagtgca
     6541 ctgcagtaca gcaggtcgac aacccttaat ataacttcgt ataatgtatg ctatacgaag
     6601 ttattaggtc tagagatccc aatacaacag atcacgtgat ctttttgtaa gatgaagttg
     6661 aagtagtgtt gcaccgtgcc aatgcaggtg gctattagat taaatatgtg atttgttcta
     6721 ttaagtttcc tgtataatta atggggagcg ctgattctct tttggtacgc ttcccatcca
     6781 gcatttctgt atctttcacc ttcaacctta ggatctctac ccttggcgaa aagtcctctg
     6841 ccaacaatga tgatatctga tccaccactt acaacttcgt cgacggttct gtactgctga
     6901 cccaatgcat cgcctttgtc gtctaaacct acacctgggg tcatgattag ccaatcaaac
     6961 ccttcttctc ttcctcccat atcgttctga gcaatgaacc caataacgaa atctttatca
     7021 ctctttgcaa tatcaacggt acccttagta tattcaccgt gtgctagaga acccttggaa
     7081 gacaattcag caagcatcaa taatcccctt ggttctttgg tgacctcttg cgcaccttgt
     7141 ttcaagccag caacaatacc agcaccagta accccgtggg cgttggtgat atcagaccat
     7201 tctgcgatac ggtaaacgcc cgatgtatat tgtaatttga ctgtgttacc gatatcggcg
     7261 aattttctgt cctcaaatat caagaacttg tatttctctg ccaatgcttt caatggaacg
     7321 acagtaccct cataactgaa atcatccaag atatcaacgt gtgttttcaa aaggcaaatg
     7381 tatggaccca acgtttcaac aagtttcaat agctcatcag tcgaacgaac gtcaagagaa
     7441 gcacacaaat tggtcttctt ttcatccatt aaacgtaaaa gtttcgatgc aaccggactt
     7501 gcatgagtct cagctctact ggtatatgat tttgtggaca tggtgcaact aattgacggg
     7561 agtgtattga cgctggcgta ctggctttca caaaatggcc caatcacaac cacatcttag
     7621 atagttgaaa tgactttaga taacatcaat tgagatgagc ttaatcatgt caaagctaaa
     7681 agtgtcacca tgaacgacaa ttcttaagca aatcacgtga tatagatcca cgaataacca
     7741 ccatttgatg ctcgaggcaa gtaatgtgtg taaaaaaatg cgttaccacc atccaatgca
     7801 gaccgatctt ctacccagaa tcacatatat ttatgtaccg agtacctttt ttctatcttc
     7861 caattgcttc tcccatatga ttgtctccgt aagctcgaaa tttctaagtt ggattttaat
     7921 cttcacgcag gatgacagtt cgatgagctt ctgaggagtg tttagaacat aatcatttat
     7981 ccatggtcta tctcttcttg tcgctttttc tcctcgatag aacctaaata aaacgagctc
     8041 tcgagaaccc ttaatataac ttcgtataat gtatgctata cgaagttatt aggtgatatc
     8101 agatccactg agtagagcac ttgaatccac tgccccggga atctcggtcg taatgatttc
     8161 tataatgacg aaaaaaaaaa aattggaaag aaaaagcttc atggccttta taaaaaggaa
     8221 ctatccaata cctcgccaga accaagtaac agtattttac ggggcacaaa tcaagaacaa
     8281 taagacagga ctgtaaagat ggacgcattg aactccaaag aacaacaaga gttccaaaaa
     8341 gtagtggaac aaaagcaaat gaaggatttc atgcgtttgt actctaatct ggtagaaaga
     8401 tgtttcacag actgtgtcaa tgacttcaca acatcaaagc taaccaataa ggaacaaaca
     8461 tgcatcatga agtgctcaga aaagttcttg aagcatagcg aacgtgtagg gcagcgtttc
     8521 caagaacaaa acgctgcctt gggacaaggc ttgggccgat aaggtgtact ggcgtatata
     8581 tatctaatta tgtatctctg gtgtagccca tttttagcat gtaaatataa agaccgagcg
     8641 gccgcgtgtt acaaccaatt aaccaattct gattagaaaa actcatcgag catcaaatga
     8701 aactgcaatt tattcatatc aggattatca ataccatatt tttgaaaaag ccgtttctgt
     8761 aatgaaggag aaaactcacc gaggcagttc cataggatgg caagatcctg gtatcggtct
     8821 gcgattccga ctcgtccaac atcaatacaa cctattaatt tcccctcgtc aaaaataagg
     8881 ttatcaagtg agaaatcacc atgagtgacg actgaatccg gtgagaatgg caaaagctta
     8941 tgcatttctt tccagacttg ttcaacaggc cagccattac gctcgtcatc aaaatcactc
     9001 gcatcaacca aaccgttatt cattcgtgat tgcgcctgag cgaggcgaaa tacgcgatcg
     9061 ctgttaaaag gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc
     9121 gcatcaacaa tattttcacc tgaatcagga tattcttcta atacctggaa tgctgttttc
     9181 ccggggatcg cagtggtgag taaccatgca tcatcaggag tacggataaa atgcttgatg
     9241 gtcggaagag gcataaattc cgtcagccag tttagtctga ccatctcatc tgtaacatca
     9301 ttggcaacgc tacctttgcc atgtttcaga aacaactctg gcgcatcggg cttcccatac
     9361 aatcgataga ttgtcgcacc tgattgcccg acattatcgc gagcccattt atacccatat
     9421 aaatcagcat ccatgttgga atttaatcgc ggcctggagc aagacgtttc ccgttgaata
     9481 tggctcataa caccccttgt attactgttt atgtaagcag acagttttat tgttcatgat
     9541 gatatatttt tatcttgtgc aatgtaacat cagagatttt gagacacaac gtggctttgt
     9601 tgaataaatc gaacttttgc tgagttgaag gatcagtcat gaccaaaatc ccttaacgtg
     9661 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     9721 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     9781 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     9841 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     9901 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     9961 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
    10021 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
    10081 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
    10141 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
    10201 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
    10261 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
    10321 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
    10381 ctgattctgt ggataaccgt gcggccgcca atgctaaatt cgagtgaaac acaggaagat
    10441 cagaaaatcc tcatttcatc catattaaca ataatttcaa atgtttattt gcattatttg
    10501 aaactaggca agacaagcaa cgaaacgttt ttgaaaattt tgagtatttt caataaattt
    10561 gtagaggact cagatattga aaaaaagcta cagcaattaa tacttgataa gaagagtatt
    10621 gagaagggca acggttcatc atctcatgga tctgcacatg aacaaacacc agagtcaaac
    10681 gacgttgaaa ttgaggctac tgcgccaatt gatgacaata cagacgatga taacaaaccg
    10741 aagttatctg atgtagaaaa ggattaaaga tgctaagaga tagtgatgat atttcataaa
    10801 taatgtaatt ctatatatgt taattacctt ttttgcgagg catatttatg gtgaaggata
    10861 agttttgacc atcaaagaag gttaatgtgg ctgtggtttc agggtccata aagcccacat
    10921 ggataacatt acccctgaat tcgcatctag actgaactgg ccgataattg cagacgaacg
    10981 ccttgccaac agggagttct tcagagacat ggaggctcaa aacgaaatta ttgacagcct
    11041 agacatcaat agtcatacaa cagaaagcga ccacccaact ttggctgata atagcgtata
    11101 aacaatgcat actttgtacg ttcaaaatac aatgcagtag atatatttat gcatattaca
    11161 tataatacat atcacatagg aagcaacagg cgcgttggac ttttaatttt cgaggaccgc
    11221 gaatccttac atcacaccca atcccccaca agtgatccc
//
