LOCUS       sequence-259445-       10759 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integration vector for optogenetic machinery 
            (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10759)
  AUTHORS   An-Adirekkun JM, Stewart CJ, Geller SH, Patel MT, Melendez J, Oakes 
            BL, Noyes MB, McClean MN
  TITLE     A yeast optogenetic toolkit (yOTK) for gene expression control in 
            Saccharomyces cerevisiae.
  JOURNAL   Biotechnol Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234.
  PUBMED    31788779
REFERENCE   2  (bases 1 to 10759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234."
COMMENT     Sequence Label: pMM0641
FEATURES             Location/Qualifiers
     source          1..10759
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             766..786
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     1014..1033
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1014..1032
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1057..1566
                     /codon_start=1
                     /product="N-terminal portion of the CIB1 transcription 
                     factor from Arabidopsis thaliana"
                     /label=CIBN
                     /note="binds to CRY2 (cryptochrome 2) that has absorbed 
                     blue light (Kennedy et al., 2010)"
                     /translation="MNGAIGGDLLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSS
                     MITGGEMDSYLSTAGLNLPMMYGETTVEGDSRLSISPETTLGTGNFKKRKFDTETKDCN
                     EKKKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKE
                     LEKTDYI"
     CDS             3086..3106
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3389..4882
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     primer_bind     5813..5836
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             5860..6570
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     terminator      6586..6773
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(6641..6665)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             7036..7839
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     CDS             complement(8481..9296)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8481..9296)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9204..9223
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     9288..9307
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      9521..10109
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10010..10029
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctgaactggc cgataattgc agacgaacga agaggatgtc caatattttt tttaaggaat
       61 aaggatactt caagactaga ttcccccctg cattcccatc agaaccgtaa accttggcgc
      121 tttccttggg aagtattcaa gaagtgcctt gtccggtttc tgtggctcac aaaccagcgc
      181 gcccgatatg gctttctttt cacttatgaa tgtaccagta cgggacaatt agaacgctcc
      241 tgtaacaatc tctttgcaaa tgtggggtta cattctaacc atgtcacact gctgacgaaa
      301 ttcaaagtaa aaaaaaatgg gaccacgtct tgagaacgat agattttctt tattttacat
      361 tgaacagtcg ttgtctcagc gcgctttatg ttttcattca tacttcatat tataaaataa
      421 caaaagaaga atttcatatt cacgcccaag aaatcaggct gctttccaaa tgcaattgac
      481 acttcattag ccatcacaca aaactctttc ttgctggagc ttcttttaaa aaagacctca
      541 gtacaccaaa cacgttaccc gacctcgtta ttttacgaca actatgataa aattctgaag
      601 aaaaaataaa aaaattttca tacttcttgc ttttatttaa accattgaat gatttctttt
      661 gaacaaaact acctgtttca ccaaaggaaa tagaaagaaa aaatcaatta gaagaaaaca
      721 aaaaacaaaa gatctatgga taaagcggaa ttaattcccg agcctccaaa aaagaagaga
      781 aaggtcgaat tgggtaccgc cgcctcggag ctccacttag acggcgagga cgtggcgatg
      841 gcgcatgccg acgcgctaga cgatttcgat ctggacatgt tgggggacgg ggattccccg
      901 ggtccgggat ttacccccca cgactccgcc ccctacggcg ctctggatat ggccgacttc
      961 gagtttgagc agatgtttac cgatgccctt ggaattgacg agtacggtgg gtttaatacg
     1021 actcactata gggcgagcgc cgaagctagc gccaccatga atggagctat aggaggtgac
     1081 cttttgctca attttcctga catgtcggtc ctagagcgcc aaagggctca cctcaagtac
     1141 ctcaatccca cctttgattc tcctctcgcc ggcttctttg ccgattcttc aatgattacc
     1201 ggcggcgaga tggacagcta tctttcgact gccggtttga atcttccgat gatgtacggt
     1261 gaaacgacgg tggaaggtga ttcaagactc tcaatttcgc cggaaacgac gcttgggact
     1321 ggaaatttca agaaacggaa gtttgataca gagactaagg attgtaatga gaagaagaag
     1381 aagatgacga tgaacagaga tgacctagta gaagaaggag aagaagagaa gtcgaaaata
     1441 acagagcaaa acaatgggag cacaaaaagc atcaagaaga tgaaacacaa agccaagaaa
     1501 gaagagaaca atttctctaa tgattcatct aaagtgacga aggaattgga gaaaacggat
     1561 tatattcatg ttcgtgcacg acgaggccaa gccactgata gtcacagcat agcagaacga
     1621 gttagaagag aaaagatcag tgagagaatg aagtttctac aagatttggt tcctggatgc
     1681 gacaagatca caggcaaagc agggatgctt gatgaaatca ttaactatgt tcagtctctt
     1741 cagagacaaa tcgagttctt atcgatgaaa ctagcaattg tgaatccaag gccggatttt
     1801 gatatggatg acatttttgc caaagaggtt gcctcaactc caatgactgt ggtgccatct
     1861 cctgaaatgg ttctttccgg ttattctcat gagatggttc actctggtta ttctagtgag
     1921 atggttaact ccggttacct tcatgtcaat ccaatgcagc aagtgaatac cagttctgat
     1981 ccattgtcat gcttcaacaa tggcgaagct ccttcgatgt gggactctca tgtgcagaat
     2041 ctctatggca atttaggagt accggtcatc gagctcgagc tgcagatgaa tcgtagatac
     2101 ggatcctaac tcgagagctt ttgattaagc cttctagtcc aaaaaacacg tttttttgtc
     2161 atttatttca ttttcttaga atagtttagt ttattcattt tatagtcacg aatgttttat
     2221 gattctatat agggttgcaa acaagcattt ttcattttat gttaaaacaa tttcaggttt
     2281 accttttatt ctgcttgtgg tgacgcgtgt atccgcccgc tcttttggtc acccatgtat
     2341 gctgccaaac cagatgtcaa cacagctaca acgaagagga tgtccaatat tttttttaag
     2401 gaataaggat acttcaagac tagattcccc cctgcattcc catcagaacc gtaaaccttg
     2461 gcgctttcct tgggaagtat tcaagaagtg ccttgtccgg tttctgtggc tcacaaacca
     2521 gcgcgcccga tatggctttc ttttcactta tgaatgtacc agtacgggac aattagaacg
     2581 ctcctgtaac aatctctttg caaatgtggg gttacattct aaccatgtca cactgctgac
     2641 gaaattcaaa gtaaaaaaaa atgggaccac gtcttgagaa cgatagattt tctttatttt
     2701 acattgaaca gtcgttgtct cagcgcgctt tatgttttca ttcatacttc atattataaa
     2761 ataacaaaag aagaatttca tattcacgcc caagaaatca ggctgctttc caaatgcaat
     2821 tgacacttca ttagccatca cacaaaactc tttcttgctg gagcttcttt taaaaaagac
     2881 ctcagtacac caaacacgtt acccgacctc gttattttac gacaactatg ataaaattct
     2941 gaagaaaaaa taaaaaaatt ttcatacttc ttgcttttat ttaaaccatt gaatgatttc
     3001 ttttgaacaa aactacctgt ttcaccaaag gaaatagaaa gaaaaaatca attagaagaa
     3061 aacaaaaaac aaaagatcta tggcccccaa gaagaagagg aaggttggta ttcacggggg
     3121 tacccgccca tatgcttgcc ctgtcgagtc ctgcgatcgc cgcttttctc gctcggatga
     3181 gcttacccgc catatccgca tccataccgg tcagaagccc ttccagtgtc gaatctgcat
     3241 gcgtaacttc agtcgtagtg accaccttac cacccacatc cgcacccaca caggcgagaa
     3301 gccttttgcc tgtgacattt gtgggaggaa gtttgccagg agtgatgaac gcaagaggca
     3361 taccaaaatc catacaggtg ctagcttcat gaagatggac aaaaagacta tagtttggtt
     3421 tagaagagat ctaaggattg aggataatcc tgcattagca gcagctgctc acgaaggatc
     3481 tgtttttcct gtcttcattt ggtgtcctga agaagaagga cagttttatc ctggaagagc
     3541 ttcaagatgg tggatgaaac aatcacttgc tcacttatct caatccttga aggctcttgg
     3601 atctgacctc actttaatca aaacccacaa cacgatttca gcgatcttgg attgtatccg
     3661 cgttaccggt gctacaaaag tcgtctttaa ccacctctat gatcctgttt cgttagttcg
     3721 ggaccatacc gtaaaggaga agctggtgga acgtgggatc tctgtgcaaa gctacaatgg
     3781 agatctattg tatgaaccgt gggagatata ctgcgaaaag ggcaaacctt ttacgagttt
     3841 caattcttac tggaagaaat gcttagatat gtcgattgaa tccgttatgc ttcctcctcc
     3901 ttggcggttg atgccaataa ctgcagcggc tgaagcgatt tgggcgtgtt cgattgaaga
     3961 actagggctg gagaatgagg ccgagaaacc gagcaatgcg ttgttaacta gagcttggag
     4021 tccaggatgg agcaatgctg ataagttact aaatgagttc atcgagaagc agttgataga
     4081 ttatgcaaag aacagcaaga aagttgttgg gaattctact tcactacttt ctccgtatct
     4141 ccatttcggg gaaataagcg tcagacacgt tttccagtgt gcccggatga aacaaattat
     4201 atgggcaaga gataagaaca gtgaaggaga agaaagtgca gatctttttc ttaggggaat
     4261 cggtttaaga gagtattctc ggtatatatg tttcaacttc ccgtttactc acgagcaatc
     4321 gttgttgagt catcttcggt ttttcccttg ggatgctgat gttgataagt tcaaggcctg
     4381 gagacaaggc aggaccggtt atccgttggt ggatgccgga atgagagagc tttgggctac
     4441 cggatggatg cataacagaa taagagtgat tgtttcaagc tttgctgtga agtttcttct
     4501 ccttccatgg aaatggggaa tgaagtattt ctgggataca cttttggatg ctgatttgga
     4561 atgtgacatc cttggctggc agtatatctc tgggagtatc cccgatggcc acgagcttga
     4621 tcgcttggac aatcccgcgt tacaaggcgc caaatatgac ccagaaggtg agtacataag
     4681 gcaatggctt cccgagcttg cgagattgcc aactgaatgg atccatcatc catgggacgc
     4741 tcctttaacc gtactcaaag cttctggtgt ggaactcgga acaaactatg cgaaacccat
     4801 tgtagacatc gacacagctc gtgagctact agctaaagct atttcaagaa cccgtgaagc
     4861 acagatcatg atcggagcag cagcccgggg atcctaactc gaggccaatt ggtgcggcaa
     4921 ttgataataa cgaaaatgtc ttttaatgat ctgggtataa tgaggaattt tccgaacgtt
     4981 tttactttat atatatatat acatgtaaca tatattctat acgctataga gaaaggaaat
     5041 ttttcaatta aaaaaaaaat agagaaagag tttcacttct tgattatcgc taacactaat
     5101 ggttgaagta ctgctacttt aattttatgc tggatgcaca cactggctta agatgacaac
     5161 gcgaggcaag ctaaacagat ctttatattg aattttcaaa aattcttact ttttttttgg
     5221 atggacgcaa agaagtttaa taatcatatt acatggcatt accaccatat acatatccat
     5281 atacatatcc atatctaatc ttacttatat gttgtggaaa tgtaaagagc cccattatct
     5341 tagcctaaaa aaaccttctc tttggaactt tcagtaatac gcttaactgc tcattgctat
     5401 attgaagtgc gcgcgcgcgt gggcgagcgt gggcgagcgt gggcgtctag accgtgcgtc
     5461 ctcgtcttca ccggtcgcgt tcctgaaacg cagatgtgcc tcgcgccgca ctgctccgaa
     5521 caataaagat tctacaatac tagcttttat ggttatgaag aggaaaaatt ggcagtaacc
     5581 tggccccaca aaccttcaaa ttaacgaatc aaattaacaa ccataggatg ataatgcgat
     5641 tagtttttta gccttatttc tggggtaatt aatcagcgaa gcgatgattt ttgatctatt
     5701 aacagatata taaatggaaa agctgcataa ccactttaac taatactttc aacattttca
     5761 gtttgtatta cttcttattc aaatgtcata aaagtatcaa caaaaaattg ttaatatacc
     5821 tctatacttt aacgtcaagg agaaaaaact ataggtacta tggtgtccaa aggagaggag
     5881 ttaatcaagg aaaacatgag aatgaaagtt gtcatggagg gctccgttaa tggtcaccaa
     5941 ttcaagtgta caggggaagg tgaaggtaat ccttacatgg gtacacaaac tatgagaatt
     6001 aaagtaattg aaggcggacc actaccattt gcatttgaca ttctggcaac gtcattcatg
     6061 tacggatcac gaactttcat caagtaccct aaaggtatac cagacttttt caagcaatct
     6121 tttccagagg gttttacatg ggaaagggtt acaagatacg aagatggggg tgtcgtcaca
     6181 gttatgcaag atacttcatt agaagatggc tgccttgtct atcatgtgca agtaagaggg
     6241 gtgaattttc cttctaacgg acctgtgatg cagaaaaaga ccaaaggttg ggaaccaaat
     6301 actgaaatga tgtacccagc tgatggaggt ttgagaggct acacacacat ggcgcttaaa
     6361 gttgatggtg gaggtcattt gtcttgtagt tttgttacca cttatcgttc taaaaagact
     6421 gttggcaata tcaaaatgcc aggaatacat gctgtagacc acagactaga aagactcgaa
     6481 gagagcgata acgaaatgtt cgttgtacag agagagcatg ccgtagccaa atttgctggc
     6541 ttaggcggtg gtatggatga attgtataag ggatcctaac tcgaggcgaa tttcttatga
     6601 tttatgattt ttattattaa ataagttata aaaaaaataa gtgtatacaa attttaaagt
     6661 gactcttagg ttttaaaacg aaaattctta ttcttgagta actctttcct gtaggtcagg
     6721 ttgctttctc aggtatagca tgaggtcgct cttattgacc acacctctac cggcatgccg
     6781 agcaaatgcc tgcaaatcgc tccccatttc gctgagcagt tacagagatg ttacgaacca
     6841 ctagtgcact gcagtacacg gtttccttga aatttttttg attcggtaat ctccgaacag
     6901 aaggaagaac gaaggaagga gcacagactt agattggtat atatacgcat atgtagtgtt
     6961 gaagaaacat gaaattgccc agtattctta acccaactgc acagaacaaa aacgtgcagg
     7021 aaacgaagat aaatcatgtc gaaagctaca tataaggaac gtgctgctac tcatcctagt
     7081 cctgttgctg ccaagctatt taatatcatg cacgaaaagc aaacaaactt gtgtgcttca
     7141 ttggatgttc gtaccaccaa ggaattactg gagttagttg aagcattagg tcccaaaatt
     7201 tgtttactaa aaacacatgt ggatatcttg actgattttt ccatggaggg cacagttaag
     7261 ccgctaaagg cattatccgc caagtacaat tttttactct tcgaggacag aaaatttgct
     7321 gacattggta atacagtcaa attgcagtac tctgcgggtg tatacagaat agcagaatgg
     7381 gcagacatta cgaatgcaca cggtgtggtg ggcccaggta ttgttagcgg tttgaagcag
     7441 gcggcagaag aagtaacaaa ggaacctaga ggccttttga tgttagcaga attgtcatgc
     7501 aagggctccc tatctactgg agaatatact aagggtactg ttgacattgc gaagagcgac
     7561 aaagattttg ttatcggctt tattgctcaa agagacatgg gtggaagaga tgaaggttac
     7621 gattggttga ttatgacacc cggtgtgggt ttagatgaca agggagatgc attgggtcaa
     7681 cagtatagaa ccgtggatga tgtggtttct acaggatctg acattattat tgttggaaga
     7741 ggactatttg caaagggaag ggatgctaag gtagagggtg aacgttacag aaaagcaggc
     7801 tgggaagcat atttgagaag atgcggccag caaaactaaa aaactgtatt ataagtaaat
     7861 gcatgtatac taaactcaca aattagagct tcaatttaat tatatcagtt attacccgag
     7921 tagagcactt gaatccactg ccccgggaat ctcggtcgta atgatttcta taatgacgaa
     7981 aaaaaaaaaa ttggaaagaa aaagcttcat ggcctttata aaaaggaact atccaatacc
     8041 tcgccagaac caagtaacag tattttacgg ggcacaaatc aagaacaata agacaggact
     8101 gtaaagatgg acgcattgaa ctccaaagaa caacaagagt tccaaaaagt agtggaacaa
     8161 aagcaaatga aggatttcat gcgtttgtac tctaatctgg tagaaagatg tttcacagac
     8221 tgtgtcaatg acttcacaac atcaaagcta accaataagg aacaaacatg catcatgaag
     8281 tgctcagaaa agttcttgaa gcatagcgaa cgtgtagggc agcgtttcca agaacaaaac
     8341 gctgccttgg gacaaggctt gggccgataa ggtgtactgg cgtatatata tctaattatg
     8401 tatctctggt gtagcccatt tttagcatgt aaatataaag accgagcggc cgcgtgttac
     8461 aaccaattaa ccaattctga ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta
     8521 ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa
     8581 aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc gattccgact
     8641 cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag
     8701 aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagcttatg catttctttc
     8761 cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa
     8821 ccgttattca ttcgtgattg cgcctgagcg aggcgaaata cgcgatcgct gttaaaagga
     8881 caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata
     8941 ttttcacctg aatcaggata ttcttctaat acctggaatg ctgttttccc ggggatcgca
     9001 gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt cggaagaggc
     9061 ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt ggcaacgcta
     9121 cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa tcgatagatt
     9181 gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa atcagcatcc
     9241 atgttggaat ttaatcgcgg cctggagcaa gacgtttccc gttgaatatg gctcataaca
     9301 ccccttgtat tactgtttat gtaagcagac agttttattg ttcatgatga tatattttta
     9361 tcttgtgcaa tgtaacatca gagattttga gacacaacgt ggctttgttg aataaatcga
     9421 acttttgctg agttgaagga tcagtcatga ccaaaatccc ttaacgtgag ttttcgttcc
     9481 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     9541 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     9601 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     9661 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     9721 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     9781 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     9841 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     9901 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     9961 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
    10021 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
    10081 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
    10141 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
    10201 ataaccgtgc ggccgccaat gctaaattcg agtgaaacac aggaagatca gaaaatcctc
    10261 atttcatcca tattaacaat aatttcaaat gtttatttgc attatttgaa actaggcaag
    10321 acaagcaacg aaacgttttt gaaaattttg agtattttca ataaatttgt agaggactca
    10381 gatattgaaa aaaagctaca gcaattaata cttgataaga agagtattga gaagggcaac
    10441 ggttcatcat ctcatggatc tgcacatgaa caaacaccag agtcaaacga cgttgaaatt
    10501 gaggctactg cgccaattga tgacaataca gacgatgata acaaaccgaa gttatctgat
    10561 gtagaaaagg attaaagatg ctaagagata gtgatgatat ttcataaata atgtaattct
    10621 atatatgtta attacctttt ttgcgaggca tatttatggt gaaggataag ttttgaccat
    10681 caaagaaggt taatgtggct gtggtttcag ggtccataaa gcccacatgg ataacattac
    10741 ccctgaattc gcatctaga
//
