LOCUS       sequence-259450-       11259 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integration vector for optogenetic machinery 
            (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2 with loxable 
            KIURA3 marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11259)
  AUTHORS   An-Adirekkun JM, Stewart CJ, Geller SH, Patel MT, Melendez J, Oakes 
            BL, Noyes MB, McClean MN
  TITLE     A yeast optogenetic toolkit (yOTK) for gene expression control in 
            Saccharomyces cerevisiae.
  JOURNAL   Biotechnol Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234.
  PUBMED    31788779
REFERENCE   2  (bases 1 to 11259)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234."
COMMENT     Sequence Label: pMM0647
FEATURES             Location/Qualifiers
     source          1..11259
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1897..1917
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2200..3693
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     primer_bind     4624..4647
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             4671..5381
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     terminator      5397..5584
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(5452..5476)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     protein_bind    5690..5723
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    7175..7208
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(7792..8607)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(7792..8607)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8515..8534
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     8599..8618
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      8832..9420
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9321..9340
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             10836..10856
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     11084..11103
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        11084..11102
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(11127..11259,1..377)
                     /codon_start=1
                     /product="N-terminal portion of the CIB1 transcription 
                     factor from Arabidopsis thaliana"
                     /label=CIBN
                     /note="binds to CRY2 (cryptochrome 2) that has absorbed 
                     blue light (Kennedy et al., 2010)"
                     /translation="MNGAIGGDLLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSS
                     MITGGEMDSYLSTAGLNLPMMYGETTVEGDSRLSISPETTLGTGNFKKRKFDTETKDCN
                     EKKKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKE
                     LEKTDYI"
ORIGIN
        1 caatgattac cggcggcgag atggacagct atctttcgac tgccggtttg aatcttccga
       61 tgatgtacgg tgaaacgacg gtggaaggtg attcaagact ctcaatttcg ccggaaacga
      121 cgcttgggac tggaaatttc aagaaacgga agtttgatac agagactaag gattgtaatg
      181 agaagaagaa gaagatgacg atgaacagag atgacctagt agaagaagga gaagaagaga
      241 agtcgaaaat aacagagcaa aacaatggga gcacaaaaag catcaagaag atgaaacaca
      301 aagccaagaa agaagagaac aatttctcta atgattcatc taaagtgacg aaggaattgg
      361 agaaaacgga ttatattcat gttcgtgcac gacgaggcca agccactgat agtcacagca
      421 tagcagaacg agttagaaga gaaaagatca gtgagagaat gaagtttcta caagatttgg
      481 ttcctggatg cgacaagatc acaggcaaag cagggatgct tgatgaaatc attaactatg
      541 ttcagtctct tcagagacaa atcgagttct tatcgatgaa actagcaatt gtgaatccaa
      601 ggccggattt tgatatggat gacatttttg ccaaagaggt tgcctcaact ccaatgactg
      661 tggtgccatc tcctgaaatg gttctttccg gttattctca tgagatggtt cactctggtt
      721 attctagtga gatggttaac tccggttacc ttcatgtcaa tccaatgcag caagtgaata
      781 ccagttctga tccattgtca tgcttcaaca atggcgaagc tccttcgatg tgggactctc
      841 atgtgcagaa tctctatggc aatttaggag taccggtcat cgagctcgag ctgcagatga
      901 atcgtagata cggatcctaa ctcgagagct tttgattaag ccttctagtc caaaaaacac
      961 gtttttttgt catttatttc attttcttag aatagtttag tttattcatt ttatagtcac
     1021 gaatgtttta tgattctata tagggttgca aacaagcatt tttcatttta tgttaaaaca
     1081 atttcaggtt taccttttat tctgcttgtg gtgacgcgtg tatccgcccg ctcttttggt
     1141 cacccatgta tgctgccaaa ccagatgtca acacagctac aacgaagagg atgtccaata
     1201 ttttttttaa ggaataagga tacttcaaga ctagattccc ccctgcattc ccatcagaac
     1261 cgtaaacctt ggcgctttcc ttgggaagta ttcaagaagt gccttgtccg gtttctgtgg
     1321 ctcacaaacc agcgcgcccg atatggcttt cttttcactt atgaatgtac cagtacggga
     1381 caattagaac gctcctgtaa caatctcttt gcaaatgtgg ggttacattc taaccatgtc
     1441 acactgctga cgaaattcaa agtaaaaaaa aatgggacca cgtcttgaga acgatagatt
     1501 ttctttattt tacattgaac agtcgttgtc tcagcgcgct ttatgttttc attcatactt
     1561 catattataa aataacaaaa gaagaatttc atattcacgc ccaagaaatc aggctgcttt
     1621 ccaaatgcaa ttgacacttc attagccatc acacaaaact ctttcttgct ggagcttctt
     1681 ttaaaaaaga cctcagtaca ccaaacacgt tacccgacct cgttatttta cgacaactat
     1741 gataaaattc tgaagaaaaa ataaaaaaat tttcatactt cttgctttta tttaaaccat
     1801 tgaatgattt cttttgaaca aaactacctg tttcaccaaa ggaaatagaa agaaaaaatc
     1861 aattagaaga aaacaaaaaa caaaagatct atggccccca agaagaagag gaaggttggt
     1921 attcacgggg gtacccgccc atatgcttgc cctgtcgagt cctgcgatcg ccgcttttct
     1981 cgctcggatg agcttacccg ccatatccgc atccataccg gtcagaagcc cttccagtgt
     2041 cgaatctgca tgcgtaactt cagtcgtagt gaccacctta ccacccacat ccgcacccac
     2101 acaggcgaga agccttttgc ctgtgacatt tgtgggagga agtttgccag gagtgatgaa
     2161 cgcaagaggc ataccaaaat ccatacaggt gctagcttca tgaagatgga caaaaagact
     2221 atagtttggt ttagaagaga tctaaggatt gaggataatc ctgcattagc agcagctgct
     2281 cacgaaggat ctgtttttcc tgtcttcatt tggtgtcctg aagaagaagg acagttttat
     2341 cctggaagag cttcaagatg gtggatgaaa caatcacttg ctcacttatc tcaatccttg
     2401 aaggctcttg gatctgacct cactttaatc aaaacccaca acacgatttc agcgatcttg
     2461 gattgtatcc gcgttaccgg tgctacaaaa gtcgtcttta accacctcta tgatcctgtt
     2521 tcgttagttc gggaccatac cgtaaaggag aagctggtgg aacgtgggat ctctgtgcaa
     2581 agctacaatg gagatctatt gtatgaaccg tgggagatat actgcgaaaa gggcaaacct
     2641 tttacgagtt tcaattctta ctggaagaaa tgcttagata tgtcgattga atccgttatg
     2701 cttcctcctc cttggcggtt gatgccaata actgcagcgg ctgaagcgat ttgggcgtgt
     2761 tcgattgaag aactagggct ggagaatgag gccgagaaac cgagcaatgc gttgttaact
     2821 agagcttgga gtccaggatg gagcaatgct gataagttac taaatgagtt catcgagaag
     2881 cagttgatag attatgcaaa gaacagcaag aaagttgttg ggaattctac ttcactactt
     2941 tctccgtatc tccatttcgg ggaaataagc gtcagacacg ttttccagtg tgcccggatg
     3001 aaacaaatta tatgggcaag agataagaac agtgaaggag aagaaagtgc agatcttttt
     3061 cttaggggaa tcggtttaag agagtattct cggtatatat gtttcaactt cccgtttact
     3121 cacgagcaat cgttgttgag tcatcttcgg tttttccctt gggatgctga tgttgataag
     3181 ttcaaggcct ggagacaagg caggaccggt tatccgttgg tggatgccgg aatgagagag
     3241 ctttgggcta ccggatggat gcataacaga ataagagtga ttgtttcaag ctttgctgtg
     3301 aagtttcttc tccttccatg gaaatgggga atgaagtatt tctgggatac acttttggat
     3361 gctgatttgg aatgtgacat ccttggctgg cagtatatct ctgggagtat ccccgatggc
     3421 cacgagcttg atcgcttgga caatcccgcg ttacaaggcg ccaaatatga cccagaaggt
     3481 gagtacataa ggcaatggct tcccgagctt gcgagattgc caactgaatg gatccatcat
     3541 ccatgggacg ctcctttaac cgtactcaaa gcttctggtg tggaactcgg aacaaactat
     3601 gcgaaaccca ttgtagacat cgacacagct cgtgagctac tagctaaagc tatttcaaga
     3661 acccgtgaag cacagatcat gatcggagca gcagcccggg gatcctaact cgaggccaat
     3721 tggtgcggca attgataata acgaaaatgt cttttaatga tctgggtata atgaggaatt
     3781 ttccgaacgt ttttacttta tatatatata tacatgtaac atatattcta tacgctatag
     3841 agaaaggaaa tttttcaatt aaaaaaaaaa tagagaaaga gtttcacttc ttgattatcg
     3901 ctaacactaa tggttgaagt actgctactt taattttatg ctggatgcac acactggctt
     3961 aagatgacaa cgcgaggcaa gctaaacaga tctttatatt gaattttcaa aaattcttac
     4021 tttttttttg gatggacgca aagaagttta ataatcatat tacatggcat taccaccata
     4081 tacatatcca tatacatatc catatctaat cttacttata tgttgtggaa atgtaaagag
     4141 ccccattatc ttagcctaaa aaaaccttct ctttggaact ttcagtaata cgcttaactg
     4201 ctcattgcta tattgaagtg cgcgcgcgcg tgggcgagcg tgggcgagcg tgggcgtcta
     4261 gaccgtgcgt cctcgtcttc accggtcgcg ttcctgaaac gcagatgtgc ctcgcgccgc
     4321 actgctccga acaataaaga ttctacaata ctagctttta tggttatgaa gaggaaaaat
     4381 tggcagtaac ctggccccac aaaccttcaa attaacgaat caaattaaca accataggat
     4441 gataatgcga ttagtttttt agccttattt ctggggtaat taatcagcga agcgatgatt
     4501 tttgatctat taacagatat ataaatggaa aagctgcata accactttaa ctaatacttt
     4561 caacattttc agtttgtatt acttcttatt caaatgtcat aaaagtatca acaaaaaatt
     4621 gttaatatac ctctatactt taacgtcaag gagaaaaaac tataggtact atggtgtcca
     4681 aaggagagga gttaatcaag gaaaacatga gaatgaaagt tgtcatggag ggctccgtta
     4741 atggtcacca attcaagtgt acaggggaag gtgaaggtaa tccttacatg ggtacacaaa
     4801 ctatgagaat taaagtaatt gaaggcggac cactaccatt tgcatttgac attctggcaa
     4861 cgtcattcat gtacggatca cgaactttca tcaagtaccc taaaggtata ccagactttt
     4921 tcaagcaatc ttttccagag ggttttacat gggaaagggt tacaagatac gaagatgggg
     4981 gtgtcgtcac agttatgcaa gatacttcat tagaagatgg ctgccttgtc tatcatgtgc
     5041 aagtaagagg ggtgaatttt ccttctaacg gacctgtgat gcagaaaaag accaaaggtt
     5101 gggaaccaaa tactgaaatg atgtacccag ctgatggagg tttgagaggc tacacacaca
     5161 tggcgcttaa agttgatggt ggaggtcatt tgtcttgtag ttttgttacc acttatcgtt
     5221 ctaaaaagac tgttggcaat atcaaaatgc caggaataca tgctgtagac cacagactag
     5281 aaagactcga agagagcgat aacgaaatgt tcgttgtaca gagagagcat gccgtagcca
     5341 aatttgctgg cttaggcggt ggtatggatg aattgtataa gggatcctaa ctcgaggcga
     5401 atttcttatg atttatgatt tttattatta aataagttat aaaaaaaata agtgtataca
     5461 aattttaaag tgactcttag gttttaaaac gaaaattctt attcttgagt aactctttcc
     5521 tgtaggtcag gttgctttct caggtatagc atgaggtcgc tcttattgac cacacctcta
     5581 ccggcatgcc gagcaaatgc ctgcaaatcg ctccccattt cgctgagcag ttacagagat
     5641 gttacgaacc actagtgcac tgcagtacag caggtcgaca acccttaata taacttcgta
     5701 taatgtatgc tatacgaagt tattaggtct agagatccca atacaacaga tcacgtgatc
     5761 tttttgtaag atgaagttga agtagtgttg caccgtgcca atgcaggtgg ctattagatt
     5821 aaatatgtga tttgttctat taagtttcct gtataattaa tggggagcgc tgattctctt
     5881 ttggtacgct tcccatccag catttctgta tctttcacct tcaaccttag gatctctacc
     5941 cttggcgaaa agtcctctgc caacaatgat gatatctgat ccaccactta caacttcgtc
     6001 gacggttctg tactgctgac ccaatgcatc gcctttgtcg tctaaaccta cacctggggt
     6061 catgattagc caatcaaacc cttcttctct tcctcccata tcgttctgag caatgaaccc
     6121 aataacgaaa tctttatcac tctttgcaat atcaacggta cccttagtat attcaccgtg
     6181 tgctagagaa cccttggaag acaattcagc aagcatcaat aatccccttg gttctttggt
     6241 gacctcttgc gcaccttgtt tcaagccagc aacaatacca gcaccagtaa ccccgtgggc
     6301 gttggtgata tcagaccatt ctgcgatacg gtaaacgccc gatgtatatt gtaatttgac
     6361 tgtgttaccg atatcggcga attttctgtc ctcaaatatc aagaacttgt atttctctgc
     6421 caatgctttc aatggaacga cagtaccctc ataactgaaa tcatccaaga tatcaacgtg
     6481 tgttttcaaa aggcaaatgt atggacccaa cgtttcaaca agtttcaata gctcatcagt
     6541 cgaacgaacg tcaagagaag cacacaaatt ggtcttcttt tcatccatta aacgtaaaag
     6601 tttcgatgca accggacttg catgagtctc agctctactg gtatatgatt ttgtggacat
     6661 ggtgcaacta attgacggga gtgtattgac gctggcgtac tggctttcac aaaatggccc
     6721 aatcacaacc acatcttaga tagttgaaat gactttagat aacatcaatt gagatgagct
     6781 taatcatgtc aaagctaaaa gtgtcaccat gaacgacaat tcttaagcaa atcacgtgat
     6841 atagatccac gaataaccac catttgatgc tcgaggcaag taatgtgtgt aaaaaaatgc
     6901 gttaccacca tccaatgcag accgatcttc tacccagaat cacatatatt tatgtaccga
     6961 gtaccttttt tctatcttcc aattgcttct cccatatgat tgtctccgta agctcgaaat
     7021 ttctaagttg gattttaatc ttcacgcagg atgacagttc gatgagcttc tgaggagtgt
     7081 ttagaacata atcatttatc catggtctat ctcttcttgt cgctttttct cctcgataga
     7141 acctaaataa aacgagctct cgagaaccct taatataact tcgtataatg tatgctatac
     7201 gaagttatta ggtgatatca gatccactga gtagagcact tgaatccact gccccgggaa
     7261 tctcggtcgt aatgatttct ataatgacga aaaaaaaaaa attggaaaga aaaagcttca
     7321 tggcctttat aaaaaggaac tatccaatac ctcgccagaa ccaagtaaca gtattttacg
     7381 gggcacaaat caagaacaat aagacaggac tgtaaagatg gacgcattga actccaaaga
     7441 acaacaagag ttccaaaaag tagtggaaca aaagcaaatg aaggatttca tgcgtttgta
     7501 ctctaatctg gtagaaagat gtttcacaga ctgtgtcaat gacttcacaa catcaaagct
     7561 aaccaataag gaacaaacat gcatcatgaa gtgctcagaa aagttcttga agcatagcga
     7621 acgtgtaggg cagcgtttcc aagaacaaaa cgctgccttg ggacaaggct tgggccgata
     7681 aggtgtactg gcgtatatat atctaattat gtatctctgg tgtagcccat ttttagcatg
     7741 taaatataaa gaccgagcgg ccgcgtgtta caaccaatta accaattctg attagaaaaa
     7801 ctcatcgagc atcaaatgaa actgcaattt attcatatca ggattatcaa taccatattt
     7861 ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg aggcagttcc ataggatggc
     7921 aagatcctgg tatcggtctg cgattccgac tcgtccaaca tcaatacaac ctattaattt
     7981 cccctcgtca aaaataaggt tatcaagtga gaaatcacca tgagtgacga ctgaatccgg
     8041 tgagaatggc aaaagcttat gcatttcttt ccagacttgt tcaacaggcc agccattacg
     8101 ctcgtcatca aaatcactcg catcaaccaa accgttattc attcgtgatt gcgcctgagc
     8161 gaggcgaaat acgcgatcgc tgttaaaagg acaattacaa acaggaatcg aatgcaaccg
     8221 gcgcaggaac actgccagcg catcaacaat attttcacct gaatcaggat attcttctaa
     8281 tacctggaat gctgttttcc cggggatcgc agtggtgagt aaccatgcat catcaggagt
     8341 acggataaaa tgcttgatgg tcggaagagg cataaattcc gtcagccagt ttagtctgac
     8401 catctcatct gtaacatcat tggcaacgct acctttgcca tgtttcagaa acaactctgg
     8461 cgcatcgggc ttcccataca atcgatagat tgtcgcacct gattgcccga cattatcgcg
     8521 agcccattta tacccatata aatcagcatc catgttggaa tttaatcgcg gcctggagca
     8581 agacgtttcc cgttgaatat ggctcataac accccttgta ttactgttta tgtaagcaga
     8641 cagttttatt gttcatgatg atatattttt atcttgtgca atgtaacatc agagattttg
     8701 agacacaacg tggctttgtt gaataaatcg aacttttgct gagttgaagg atcagtcatg
     8761 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     8821 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     8881 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     8941 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     9001 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     9061 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     9121 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     9181 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     9241 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     9301 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     9361 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     9421 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     9481 ttctttcctg cgttatcccc tgattctgtg gataaccgtg cggccgccaa tgctaaattc
     9541 gagtgaaaca caggaagatc agaaaatcct catttcatcc atattaacaa taatttcaaa
     9601 tgtttatttg cattatttga aactaggcaa gacaagcaac gaaacgtttt tgaaaatttt
     9661 gagtattttc aataaatttg tagaggactc agatattgaa aaaaagctac agcaattaat
     9721 acttgataag aagagtattg agaagggcaa cggttcatca tctcatggat ctgcacatga
     9781 acaaacacca gagtcaaacg acgttgaaat tgaggctact gcgccaattg atgacaatac
     9841 agacgatgat aacaaaccga agttatctga tgtagaaaag gattaaagat gctaagagat
     9901 agtgatgata tttcataaat aatgtaattc tatatatgtt aattaccttt tttgcgaggc
     9961 atatttatgg tgaaggataa gttttgacca tcaaagaagg ttaatgtggc tgtggtttca
    10021 gggtccataa agcccacatg gataacatta cccctgaatt cgcatctaga ctgaactggc
    10081 cgataattgc agacgaacga agaggatgtc caatattttt tttaaggaat aaggatactt
    10141 caagactaga ttcccccctg cattcccatc agaaccgtaa accttggcgc tttccttggg
    10201 aagtattcaa gaagtgcctt gtccggtttc tgtggctcac aaaccagcgc gcccgatatg
    10261 gctttctttt cacttatgaa tgtaccagta cgggacaatt agaacgctcc tgtaacaatc
    10321 tctttgcaaa tgtggggtta cattctaacc atgtcacact gctgacgaaa ttcaaagtaa
    10381 aaaaaaatgg gaccacgtct tgagaacgat agattttctt tattttacat tgaacagtcg
    10441 ttgtctcagc gcgctttatg ttttcattca tacttcatat tataaaataa caaaagaaga
    10501 atttcatatt cacgcccaag aaatcaggct gctttccaaa tgcaattgac acttcattag
    10561 ccatcacaca aaactctttc ttgctggagc ttcttttaaa aaagacctca gtacaccaaa
    10621 cacgttaccc gacctcgtta ttttacgaca actatgataa aattctgaag aaaaaataaa
    10681 aaaattttca tacttcttgc ttttatttaa accattgaat gatttctttt gaacaaaact
    10741 acctgtttca ccaaaggaaa tagaaagaaa aaatcaatta gaagaaaaca aaaaacaaaa
    10801 gatctatgga taaagcggaa ttaattcccg agcctccaaa aaagaagaga aaggtcgaat
    10861 tgggtaccgc cgcctcggag ctccacttag acggcgagga cgtggcgatg gcgcatgccg
    10921 acgcgctaga cgatttcgat ctggacatgt tgggggacgg ggattccccg ggtccgggat
    10981 ttacccccca cgactccgcc ccctacggcg ctctggatat ggccgacttc gagtttgagc
    11041 agatgtttac cgatgccctt ggaattgacg agtacggtgg gtttaatacg actcactata
    11101 gggcgagcgc cgaagctagc gccaccatga atggagctat aggaggtgac cttttgctca
    11161 attttcctga catgtcggtc ctagagcgcc aaagggctca cctcaagtac ctcaatccca
    11221 cctttgattc tcctctcgcc ggcttctttg ccgattctt
//
