LOCUS       sequence-259453-       10767 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Control for optogenetic machinery 
            (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pWEAK-mRUBY2 
            reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10767)
  AUTHORS   An-Adirekkun JM, Stewart CJ, Geller SH, Patel MT, Melendez J, Oakes 
            BL, Noyes MB, McClean MN
  TITLE     A yeast optogenetic toolkit (yOTK) for gene expression control in 
            Saccharomyces cerevisiae.
  JOURNAL   Biotechnol Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234.
  PUBMED    31788779
REFERENCE   2  (bases 1 to 10767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234."
COMMENT     Sequence Label: pMM0653
FEATURES             Location/Qualifiers
     source          1..10767
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     316..335
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     400..419
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      633..1221
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1122..1141
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             2637..2657
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     2885..2904
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2885..2903
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2928..3437
                     /codon_start=1
                     /product="N-terminal portion of the CIB1 transcription 
                     factor from Arabidopsis thaliana"
                     /label=CIBN
                     /note="binds to CRY2 (cryptochrome 2) that has absorbed 
                     blue light (Kennedy et al., 2010)"
                     /translation="MNGAIGGDLLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSS
                     MITGGEMDSYLSTAGLNLPMMYGETTVEGDSRLSISPETTLGTGNFKKRKFDTETKDCN
                     EKKKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKE
                     LEKTDYI"
     CDS             4957..4977
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5260..6753
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     CDS             7739..8449
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     terminator      8465..8652
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(8520..8544)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             8915..9718
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     CDS             complement(join(10360..10767,1..408))
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(join(10360..10767,1..408))
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa tattttcacc
       61 tgaatcagga tattcttcta atacctggaa tgctgttttc ccggggatcg cagtggtgag
      121 taaccatgca tcatcaggag tacggataaa atgcttgatg gtcggaagag gcataaattc
      181 cgtcagccag tttagtctga ccatctcatc tgtaacatca ttggcaacgc tacctttgcc
      241 atgtttcaga aacaactctg gcgcatcggg cttcccatac aatcgataga ttgtcgcacc
      301 tgattgcccg acattatcgc gagcccattt atacccatat aaatcagcat ccatgttgga
      361 atttaatcgc ggcctggagc aagacgtttc ccgttgaata tggctcataa caccccttgt
      421 attactgttt atgtaagcag acagttttat tgttcatgat gatatatttt tatcttgtgc
      481 aatgtaacat cagagatttt gagacacaac gtggctttgt tgaataaatc gaacttttgc
      541 tgagttgaag gatcagtcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
      601 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
      661 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
      721 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
      781 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
      841 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
      901 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
      961 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     1021 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     1081 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     1141 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     1201 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     1261 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     1321 gcggccgcca atgctaaatt cgagtgaaac acaggaagat cagaaaatcc tcatttcatc
     1381 catattaaca ataatttcaa atgtttattt gcattatttg aaactaggca agacaagcaa
     1441 cgaaacgttt ttgaaaattt tgagtatttt caataaattt gtagaggact cagatattga
     1501 aaaaaagcta cagcaattaa tacttgataa gaagagtatt gagaagggca acggttcatc
     1561 atctcatgga tctgcacatg aacaaacacc agagtcaaac gacgttgaaa ttgaggctac
     1621 tgcgccaatt gatgacaata cagacgatga taacaaaccg aagttatctg atgtagaaaa
     1681 ggattaaaga tgctaagaga tagtgatgat atttcataaa taatgtaatt ctatatatgt
     1741 taattacctt ttttgcgagg catatttatg gtgaaggata agttttgacc atcaaagaag
     1801 gttaatgtgg ctgtggtttc agggtccata aagcccacat ggataacatt acccctgaat
     1861 tcgcatctag actgaactgg ccgataattg cagacgaacg aagaggatgt ccaatatttt
     1921 ttttaaggaa taaggatact tcaagactag attcccccct gcattcccat cagaaccgta
     1981 aaccttggcg ctttccttgg gaagtattca agaagtgcct tgtccggttt ctgtggctca
     2041 caaaccagcg cgcccgatat ggctttcttt tcacttatga atgtaccagt acgggacaat
     2101 tagaacgctc ctgtaacaat ctctttgcaa atgtggggtt acattctaac catgtcacac
     2161 tgctgacgaa attcaaagta aaaaaaaatg ggaccacgtc ttgagaacga tagattttct
     2221 ttattttaca ttgaacagtc gttgtctcag cgcgctttat gttttcattc atacttcata
     2281 ttataaaata acaaaagaag aatttcatat tcacgcccaa gaaatcaggc tgctttccaa
     2341 atgcaattga cacttcatta gccatcacac aaaactcttt cttgctggag cttcttttaa
     2401 aaaagacctc agtacaccaa acacgttacc cgacctcgtt attttacgac aactatgata
     2461 aaattctgaa gaaaaaataa aaaaattttc atacttcttg cttttattta aaccattgaa
     2521 tgatttcttt tgaacaaaac tacctgtttc accaaaggaa atagaaagaa aaaatcaatt
     2581 agaagaaaac aaaaaacaaa agatctatgg ataaagcgga attaattccc gagcctccaa
     2641 aaaagaagag aaaggtcgaa ttgggtaccg ccgcctcgga gctccactta gacggcgagg
     2701 acgtggcgat ggcgcatgcc gacgcgctag acgatttcga tctggacatg ttgggggacg
     2761 gggattcccc gggtccggga tttacccccc acgactccgc cccctacggc gctctggata
     2821 tggccgactt cgagtttgag cagatgttta ccgatgccct tggaattgac gagtacggtg
     2881 ggtttaatac gactcactat agggcgagcg ccgaagctag cgccaccatg aatggagcta
     2941 taggaggtga ccttttgctc aattttcctg acatgtcggt cctagagcgc caaagggctc
     3001 acctcaagta cctcaatccc acctttgatt ctcctctcgc cggcttcttt gccgattctt
     3061 caatgattac cggcggcgag atggacagct atctttcgac tgccggtttg aatcttccga
     3121 tgatgtacgg tgaaacgacg gtggaaggtg attcaagact ctcaatttcg ccggaaacga
     3181 cgcttgggac tggaaatttc aagaaacgga agtttgatac agagactaag gattgtaatg
     3241 agaagaagaa gaagatgacg atgaacagag atgacctagt agaagaagga gaagaagaga
     3301 agtcgaaaat aacagagcaa aacaatggga gcacaaaaag catcaagaag atgaaacaca
     3361 aagccaagaa agaagagaac aatttctcta atgattcatc taaagtgacg aaggaattgg
     3421 agaaaacgga ttatattcat gttcgtgcac gacgaggcca agccactgat agtcacagca
     3481 tagcagaacg agttagaaga gaaaagatca gtgagagaat gaagtttcta caagatttgg
     3541 ttcctggatg cgacaagatc acaggcaaag cagggatgct tgatgaaatc attaactatg
     3601 ttcagtctct tcagagacaa atcgagttct tatcgatgaa actagcaatt gtgaatccaa
     3661 ggccggattt tgatatggat gacatttttg ccaaagaggt tgcctcaact ccaatgactg
     3721 tggtgccatc tcctgaaatg gttctttccg gttattctca tgagatggtt cactctggtt
     3781 attctagtga gatggttaac tccggttacc ttcatgtcaa tccaatgcag caagtgaata
     3841 ccagttctga tccattgtca tgcttcaaca atggcgaagc tccttcgatg tgggactctc
     3901 atgtgcagaa tctctatggc aatttaggag taccggtcat cgagctcgag ctgcagatga
     3961 atcgtagata cggatcctaa ctcgagagct tttgattaag ccttctagtc caaaaaacac
     4021 gtttttttgt catttatttc attttcttag aatagtttag tttattcatt ttatagtcac
     4081 gaatgtttta tgattctata tagggttgca aacaagcatt tttcatttta tgttaaaaca
     4141 atttcaggtt taccttttat tctgcttgtg gtgacgcgtg tatccgcccg ctcttttggt
     4201 cacccatgta tgctgccaaa ccagatgtca acacagctac aacgaagagg atgtccaata
     4261 ttttttttaa ggaataagga tacttcaaga ctagattccc ccctgcattc ccatcagaac
     4321 cgtaaacctt ggcgctttcc ttgggaagta ttcaagaagt gccttgtccg gtttctgtgg
     4381 ctcacaaacc agcgcgcccg atatggcttt cttttcactt atgaatgtac cagtacggga
     4441 caattagaac gctcctgtaa caatctcttt gcaaatgtgg ggttacattc taaccatgtc
     4501 acactgctga cgaaattcaa agtaaaaaaa aatgggacca cgtcttgaga acgatagatt
     4561 ttctttattt tacattgaac agtcgttgtc tcagcgcgct ttatgttttc attcatactt
     4621 catattataa aataacaaaa gaagaatttc atattcacgc ccaagaaatc aggctgcttt
     4681 ccaaatgcaa ttgacacttc attagccatc acacaaaact ctttcttgct ggagcttctt
     4741 ttaaaaaaga cctcagtaca ccaaacacgt tacccgacct cgttatttta cgacaactat
     4801 gataaaattc tgaagaaaaa ataaaaaaat tttcatactt cttgctttta tttaaaccat
     4861 tgaatgattt cttttgaaca aaactacctg tttcaccaaa ggaaatagaa agaaaaaatc
     4921 aattagaaga aaacaaaaaa caaaagatct atggccccca agaagaagag gaaggttggt
     4981 attcacgggg gtacccgccc atatgcttgc cctgtcgagt cctgcgatcg ccgcttttct
     5041 cgctcggatg agcttacccg ccatatccgc atccataccg gtcagaagcc cttccagtgt
     5101 cgaatctgca tgcgtaactt cagtcgtagt gaccacctta ccacccacat ccgcacccac
     5161 acaggcgaga agccttttgc ctgtgacatt tgtgggagga agtttgccag gagtgatgaa
     5221 cgcaagaggc ataccaaaat ccatacaggt gctagcttca tgaagatgga caaaaagact
     5281 atagtttggt ttagaagaga tctaaggatt gaggataatc ctgcattagc agcagctgct
     5341 cacgaaggat ctgtttttcc tgtcttcatt tggtgtcctg aagaagaagg acagttttat
     5401 cctggaagag cttcaagatg gtggatgaaa caatcacttg ctcacttatc tcaatccttg
     5461 aaggctcttg gatctgacct cactttaatc aaaacccaca acacgatttc agcgatcttg
     5521 gattgtatcc gcgttaccgg tgctacaaaa gtcgtcttta accacctcta tgatcctgtt
     5581 tcgttagttc gggaccatac cgtaaaggag aagctggtgg aacgtgggat ctctgtgcaa
     5641 agctacaatg gagatctatt gtatgaaccg tgggagatat actgcgaaaa gggcaaacct
     5701 tttacgagtt tcaattctta ctggaagaaa tgcttagata tgtcgattga atccgttatg
     5761 cttcctcctc cttggcggtt gatgccaata actgcagcgg ctgaagcgat ttgggcgtgt
     5821 tcgattgaag aactagggct ggagaatgag gccgagaaac cgagcaatgc gttgttaact
     5881 agagcttgga gtccaggatg gagcaatgct gataagttac taaatgagtt catcgagaag
     5941 cagttgatag attatgcaaa gaacagcaag aaagttgttg ggaattctac ttcactactt
     6001 tctccgtatc tccatttcgg ggaaataagc gtcagacacg ttttccagtg tgcccggatg
     6061 aaacaaatta tatgggcaag agataagaac agtgaaggag aagaaagtgc agatcttttt
     6121 cttaggggaa tcggtttaag agagtattct cggtatatat gtttcaactt cccgtttact
     6181 cacgagcaat cgttgttgag tcatcttcgg tttttccctt gggatgctga tgttgataag
     6241 ttcaaggcct ggagacaagg caggaccggt tatccgttgg tggatgccgg aatgagagag
     6301 ctttgggcta ccggatggat gcataacaga ataagagtga ttgtttcaag ctttgctgtg
     6361 aagtttcttc tccttccatg gaaatgggga atgaagtatt tctgggatac acttttggat
     6421 gctgatttgg aatgtgacat ccttggctgg cagtatatct ctgggagtat ccccgatggc
     6481 cacgagcttg atcgcttgga caatcccgcg ttacaaggcg ccaaatatga cccagaaggt
     6541 gagtacataa ggcaatggct tcccgagctt gcgagattgc caactgaatg gatccatcat
     6601 ccatgggacg ctcctttaac cgtactcaaa gcttctggtg tggaactcgg aacaaactat
     6661 gcgaaaccca ttgtagacat cgacacagct cgtgagctac tagctaaagc tatttcaaga
     6721 acccgtgaag cacagatcat gatcggagca gcagcccggg gatcctaact cgaggccaat
     6781 tggtgcggca attgataata acgaaaatgt cttttaatga tctgggtata atgaggaatt
     6841 ttccgaacgt ttttacttta tatatatata tacatgtaac atatattcta tacgctatag
     6901 agaaaggaaa tttttcaatt aaaaaaaaaa tagagaaaga gtttcacttc ttgattatcg
     6961 ctaacactaa tggttgaagt actgctactt taattttatg ctggatgcac acactggctt
     7021 aagatgacaa cggtgttgtt atccgataca accggatatt tttcttttaa tgagtctaaa
     7081 ccgtgatagc ttcaggttaa tacaatcaaa aaaagctcaa atattctttt aatgccgcgt
     7141 tcacagattc caattgaata caactaggta gttcattata tgaagccttt gctactattt
     7201 ttcactatag tctgccttca ccttaatgca gacatccaca tattttaatc actttaaaat
     7261 aaaaaggaag atatattaga agctatgatc caatctgtaa gccagattaa aattcacgaa
     7321 ctcttctttc atttgaattg aatgctttga gttggggtag attatcgcaa attactcatc
     7381 acatttattg actacgaact tgctgatgtc ctttttttat ttatattttt cttcagtgaa
     7441 gcgatttttt ttttacacag accaagacgg aaaaaagtag ctaaggaaga aaacaaaatc
     7501 atgaaaaaaa tgtgaagtga tcatgcacat cgcatcaact taaacattgg cttagagata
     7561 tatagagtta gagtttacgg caacctttaa gcaccaatac cttttggcat agtctaaaga
     7621 cctggttctt aattttaaac aaatttaact aaagatttcc ctatcaaaga agtaacgagt
     7681 tgacagattt tctcaaaata aatcgatact gcatttctag gcatatccag cgagatctat
     7741 ggtgtccaaa ggagaggagt taatcaagga aaacatgaga atgaaagttg tcatggaggg
     7801 ctccgttaat ggtcaccaat tcaagtgtac aggggaaggt gaaggtaatc cttacatggg
     7861 tacacaaact atgagaatta aagtaattga aggcggacca ctaccatttg catttgacat
     7921 tctggcaacg tcattcatgt acggatcacg aactttcatc aagtacccta aaggtatacc
     7981 agactttttc aagcaatctt ttccagaggg ttttacatgg gaaagggtta caagatacga
     8041 agatgggggt gtcgtcacag ttatgcaaga tacttcatta gaagatggct gccttgtcta
     8101 tcatgtgcaa gtaagagggg tgaattttcc ttctaacgga cctgtgatgc agaaaaagac
     8161 caaaggttgg gaaccaaata ctgaaatgat gtacccagct gatggaggtt tgagaggcta
     8221 cacacacatg gcgcttaaag ttgatggtgg aggtcatttg tcttgtagtt ttgttaccac
     8281 ttatcgttct aaaaagactg ttggcaatat caaaatgcca ggaatacatg ctgtagacca
     8341 cagactagaa agactcgaag agagcgataa cgaaatgttc gttgtacaga gagagcatgc
     8401 cgtagccaaa tttgctggct taggcggtgg tatggatgaa ttgtataagg gatcctaact
     8461 cgaggcgaat ttcttatgat ttatgatttt tattattaaa taagttataa aaaaaataag
     8521 tgtatacaaa ttttaaagtg actcttaggt tttaaaacga aaattcttat tcttgagtaa
     8581 ctctttcctg taggtcaggt tgctttctca ggtatagcat gaggtcgctc ttattgacca
     8641 cacctctacc ggcatgccga gcaaatgcct gcaaatcgct ccccatttcg ctgagcagtt
     8701 acagagatgt tacgaaccac tagtgcactg cagtacacgg tttccttgaa atttttttga
     8761 ttcggtaatc tccgaacaga aggaagaacg aaggaaggag cacagactta gattggtata
     8821 tatacgcata tgtagtgttg aagaaacatg aaattgccca gtattcttaa cccaactgca
     8881 cagaacaaaa acgtgcagga aacgaagata aatcatgtcg aaagctacat ataaggaacg
     8941 tgctgctact catcctagtc ctgttgctgc caagctattt aatatcatgc acgaaaagca
     9001 aacaaacttg tgtgcttcat tggatgttcg taccaccaag gaattactgg agttagttga
     9061 agcattaggt cccaaaattt gtttactaaa aacacatgtg gatatcttga ctgatttttc
     9121 catggagggc acagttaagc cgctaaaggc attatccgcc aagtacaatt ttttactctt
     9181 cgaggacaga aaatttgctg acattggtaa tacagtcaaa ttgcagtact ctgcgggtgt
     9241 atacagaata gcagaatggg cagacattac gaatgcacac ggtgtggtgg gcccaggtat
     9301 tgttagcggt ttgaagcagg cggcagaaga agtaacaaag gaacctagag gccttttgat
     9361 gttagcagaa ttgtcatgca agggctccct atctactgga gaatatacta agggtactgt
     9421 tgacattgcg aagagcgaca aagattttgt tatcggcttt attgctcaaa gagacatggg
     9481 tggaagagat gaaggttacg attggttgat tatgacaccc ggtgtgggtt tagatgacaa
     9541 gggagatgca ttgggtcaac agtatagaac cgtggatgat gtggtttcta caggatctga
     9601 cattattatt gttggaagag gactatttgc aaagggaagg gatgctaagg tagagggtga
     9661 acgttacaga aaagcaggct gggaagcata tttgagaaga tgcggccagc aaaactaaaa
     9721 aactgtatta taagtaaatg catgtatact aaactcacaa attagagctt caatttaatt
     9781 atatcagtta ttacccgagt agagcacttg aatccactgc cccgggaatc tcggtcgtaa
     9841 tgatttctat aatgacgaaa aaaaaaaaat tggaaagaaa aagcttcatg gcctttataa
     9901 aaaggaacta tccaatacct cgccagaacc aagtaacagt attttacggg gcacaaatca
     9961 agaacaataa gacaggactg taaagatgga cgcattgaac tccaaagaac aacaagagtt
    10021 ccaaaaagta gtggaacaaa agcaaatgaa ggatttcatg cgtttgtact ctaatctggt
    10081 agaaagatgt ttcacagact gtgtcaatga cttcacaaca tcaaagctaa ccaataagga
    10141 acaaacatgc atcatgaagt gctcagaaaa gttcttgaag catagcgaac gtgtagggca
    10201 gcgtttccaa gaacaaaacg ctgccttggg acaaggcttg ggccgataag gtgtactggc
    10261 gtatatatat ctaattatgt atctctggtg tagcccattt ttagcatgta aatataaaga
    10321 ccgagcggcc gcgtgttaca accaattaac caattctgat tagaaaaact catcgagcat
    10381 caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg
    10441 tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta
    10501 tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa
    10561 aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa
    10621 aagcttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa
    10681 atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga ggcgaaatac
    10741 gcgatcgctg ttaaaaggac aattaca
//
