LOCUS       sequence-25947-e         756 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral Luciferase shRNA (H1/TO promoter), 3'LTR insertion, Neo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pQCXIN X2/pTER shLUC (w347-1)
FEATURES             Location/Qualifiers
     source          1..756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    28..46
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    complement(179..203)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agnagcttgg attgggatct tataagttcc ctatcagtga tagagatccc cttacgctga
       61 gtacttcgag tgtgctgtcc tcgaagtact cagcgtaagt ttttggaaaa gcttggtacc
      121 gagctcggat ccactagtcc agtgtggtgg aattcgcggc cgcactcgag atatctagac
      181 ccagctttct tgtacaaagt ggttgatatc cagcacagtg gcggccgctc gagtctagct
      241 tgccaaacct acaggtgggg tctttcattc cccccttttt ctggagacta aataaaatct
      301 tttattttat ctatggctcg tactctatag gcttcagctg gtgatattgt tgagtcaaaa
      361 ctagagcctg gaccactgat atcctgtctt taacaaattg gactaatcga taagcttatc
      421 tctacactgg ctcgatggag tcagcctagt gtccctcgga aagccttgag gtccctttgg
      481 agcagttctg cactcggcct ctgcagcgct gcagccctcg agtgggtggt gagcagctcg
      541 ccgtcgcgca gcaggcgcca gcgctccatc cacgcagtga cctcaggcgg aaaacgggaa
      601 gacacactca tgagatgcct gcaagcaatt cgttctgtat caggcgcagg agcgtcccgt
      661 ccgggtcgac tctagccgat cccgctcaga agaactcgtc aagaaggcga tagaaggcga
      721 tgcgctgcga atcgggagcg gcgataccgt aaagca
//
