LOCUS       sequence-259480-       10928 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-T base editing using LpCDA1L1_4 fused to C terminal of nCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10928)
  AUTHORS   Cheng TL, Li S, Yuan B, Wang X, Zhou W, Qiu Z
  TITLE     Expanding C-T base editing toolkit with diversified cytidine 
            deaminases.
  JOURNAL   Nat Commun. 2019 Aug 9;10(1):3612. doi: 10.1038/s41467-019-11562-6.
  PUBMED    31399578
REFERENCE   2  (bases 1 to 10928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-08-9"; volume: "10(1):3612. doi"; pages: " 
            10.1038/s41467-019-11562-6"
COMMENT     Sequence Label: C-13-BE
FEATURES             Location/Qualifiers
     source          1..10928
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..249
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1..21
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     172..191
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     intron          409..1347
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1304..1324
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1375..1440
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             1447..1467
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(1492..1515,1516..1518,1519..5592)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             1492..5592
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5593..5640
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5854..5919
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             6589..6840
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="MTNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHT
                     AYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6880..7125
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="NLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTAY
                     DESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             7138..7158
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7174..7227
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(7228..7230,7231..7941)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(7228..7230,7231..7941)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(7531..7550)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7878..7899
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(7969..7986)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7975..8182
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   8191..8331
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   8191..8320
                     /label=AAV2 ITR
     rep_origin      8406..8861
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8493..8512)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8703..8724
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8878..8897)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8997..9019
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9057..9075)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9143..9247
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9248..9316,9317..10108)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9466..9485)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10279..10867
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10768..10787
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacacca attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat
      301 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta
      361 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg
      421 tgtggttccc gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc ttgaattact
      481 tccacctggc tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag
      541 agttcgaggc cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggcct
      601 gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc
      661 gataagtctc tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa
      721 gatagtcttg taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg
      781 ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg
      841 cggccaccga gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc
      901 ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt
      961 gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg
     1021 cggcgctcgg gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc
     1081 tcagccgtcg cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag
     1141 ttctcgagct tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag
     1201 tttccccaca ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc
     1261 tccttggaat ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg
     1321 gttcaaagtt tttttcttcc atttcaggtg tcgtgaggtg tcgtgagcgc catggactat
     1381 aaggaccacg acggagacta caaggatcat gatattgatt acaaagacga tgacgataag
     1441 atggccccaa agaagaagcg gaaggtcggt atccacggag tcccagcagc cgacaagaag
     1501 tacagcatcg gcctggccat cggcaccaac tctgtgggct gggccgtgat caccgacgag
     1561 tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag
     1621 aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc cacccggctg
     1681 aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta tctgcaagag
     1741 atcttcagca acgagatggc caaggtggac gacagcttct tccacagact ggaagagtcc
     1801 ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa catcgtggac
     1861 gaggtggcct accacgagaa gtaccccacc atctaccacc tgagaaagaa actggtggac
     1921 agcaccgaca aggccgacct gcggctgatc tatctggccc tggcccacat gatcaagttc
     1981 cggggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt ggacaagctg
     2041 ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aaaaccccat caacgccagc
     2101 ggcgtggacg ccaaggccat cctgtctgcc agactgagca agagcagacg gctggaaaat
     2161 ctgatcgccc agctgcccgg cgagaagaag aatggcctgt tcggcaacct gattgccctg
     2221 agcctgggcc tgacccccaa cttcaagagc aacttcgacc tggccgagga tgccaaactg
     2281 cagctgagca aggacaccta cgacgacgac ctggacaacc tgctggccca gatcggcgac
     2341 cagtacgccg acctgtttct ggccgccaag aacctgtccg acgccatcct gctgagcgac
     2401 atcctgagag tgaacaccga gatcaccaag gcccccctga gcgcctctat gatcaagaga
     2461 tacgacgagc accaccagga cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct
     2521 gagaagtaca aagagatttt cttcgaccag agcaagaacg gctacgccgg ctacattgac
     2581 ggcggagcca gccaggaaga gttctacaag ttcatcaagc ccatcctgga aaagatggac
     2641 ggcaccgagg aactgctcgt gaagctgaac agagaggacc tgctgcggaa gcagcggacc
     2701 ttcgacaacg gcagcatccc ccaccagatc cacctgggag agctgcacgc cattctgcgg
     2761 cggcaggaag atttttaccc attcctgaag gacaaccggg aaaagatcga gaagatcctg
     2821 accttccgca tcccctacta cgtgggccct ctggccaggg gaaacagcag attcgcctgg
     2881 atgaccagaa agagcgagga aaccatcacc ccctggaact tcgaggaagt ggtggacaag
     2941 ggcgcttccg cccagagctt catcgagcgg atgaccaact tcgataagaa cctgcccaac
     3001 gagaaggtgc tgcccaagca cagcctgctg tacgagtact tcaccgtgta taacgagctg
     3061 accaaagtga aatacgtgac cgagggaatg agaaagcccg ccttcctgag cggcgagcag
     3121 aaaaaggcca tcgtggacct gctgttcaag accaaccgga aagtgaccgt gaagcagctg
     3181 aaagaggact acttcaagaa aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa
     3241 gatcggttca acgcctccct gggcacatac cacgatctgc tgaaaattat caaggacaag
     3301 gacttcctgg acaatgagga aaacgaggac attctggaag atatcgtgct gaccctgaca
     3361 ctgtttgagg acagagagat gatcgaggaa cggctgaaaa cctatgccca cctgttcgac
     3421 gacaaagtga tgaagcagct gaagcggcgg agatacaccg gctggggcag gctgagccgg
     3481 aagctgatca acggcatccg ggacaagcag tccggcaaga caatcctgga tttcctgaag
     3541 tccgacggct tcgccaacag aaacttcatg cagctgatcc acgacgacag cctgaccttt
     3601 aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg atagcctgca cgagcacatt
     3661 gccaatctgg ccggcagccc cgccattaag aagggcatcc tgcagacagt gaaggtggtg
     3721 gacgagctcg tgaaagtgat gggccggcac aagcccgaga acatcgtgat cgaaatggcc
     3781 agagagaacc agaccaccca gaagggacag aagaacagcc gcgagagaat gaagcggatc
     3841 gaagagggca tcaaagagct gggcagccag atcctgaaag aacaccccgt ggaaaacacc
     3901 cagctgcaga acgagaagct gtacctgtac tacctgcaga atgggcggga tatgtacgtg
     3961 gaccaggaac tggacatcaa ccggctgtcc gactacgatg tggaccatat cgtgcctcag
     4021 agctttctga aggacgactc catcgacaac aaggtgctga ccagaagcga caagaaccgg
     4081 ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa ctactggcgg
     4141 cagctgctga acgccaagct gattacccag agaaagttcg acaatctgac caaggccgag
     4201 agaggcggcc tgagcgaact ggataaggcc ggcttcatca agagacagct ggtggaaacc
     4261 cggcagatca caaagcacgt ggcacagatc ctggactccc ggatgaacac taagtacgac
     4321 gagaatgaca agctgatccg ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc
     4381 gatttccgga aggatttcca gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc
     4441 cacgacgcct acctgaacgc cgtcgtggga accgccctga tcaaaaagta ccctaagctg
     4501 gaaagcgagt tcgtgtacgg cgactacaag gtgtacgacg tgcggaagat gatcgccaag
     4561 agcgagcagg aaatcggcaa ggctaccgcc aagtacttct tctacagcaa catcatgaac
     4621 tttttcaaga ccgagattac cctggccaac ggcgagatcc ggaagcggcc tctgatcgag
     4681 acaaacggcg aaaccgggga gatcgtgtgg gataagggcc gggattttgc caccgtgcgg
     4741 aaagtgctga gcatgcccca agtgaatatc gtgaaaaaga ccgaggtgca gacaggcggc
     4801 ttcagcaaag agtctatcct gcccaagagg aacagcgata agctgatcgc cagaaagaag
     4861 gactgggacc ctaagaagta cggcggcttc gacagcccca ccgtggccta ttctgtgctg
     4921 gtggtggcca aagtggaaaa gggcaagtcc aagaaactga agagtgtgaa agagctgctg
     4981 gggatcacca tcatggaaag aagcagcttc gagaagaatc ccatcgactt tctggaagcc
     5041 aagggctaca aagaagtgaa aaaggacctg atcatcaagc tgcctaagta ctccctgttc
     5101 gagctggaaa acggccggaa gagaatgctg gcctctgccg gcgaactgca gaagggaaac
     5161 gaactggccc tgccctccaa atatgtgaac ttcctgtacc tggccagcca ctatgagaag
     5221 ctgaagggct cccccgagga taatgagcag aaacagctgt ttgtggaaca gcacaagcac
     5281 tacctggacg agatcatcga gcagatcagc gagttctcca agagagtgat cctggccgac
     5341 gctaatctgg acaaagtgct gtccgcctac aacaagcacc gggataagcc catcagagag
     5401 caggccgaga atatcatcca cctgtttacc ctgaccaatc tgggagcccc tgccgccttc
     5461 aagtactttg acaccaccat cgaccggaag aggtacacca gcaccaaaga ggtgctggac
     5521 gccaccctga tccaccagag catcaccggc ctgtacgaga cacggatcga cctgtctcag
     5581 ctgggaggcg acaaaaggcc ggcggccacg aaaaaggccg gccaggcaaa aaagaaaaag
     5641 gaattgggtg gaggaggtac aggcggtgga ggctcagcag aatacgtacg agctctgttt
     5701 gacttcaatg ggaatgacga ggaggatctc ccctttaaga agggcgatat tctccgcatc
     5761 agagataagc ccgaagaaca atggtggaat gccgaggata gcgaagggaa aaggggcatg
     5821 attctggtgc cttatgtgga gaaatattcc ggtgactaca aagaccatga tggggattac
     5881 aaagaccacg acatcgacta caaagacgac gacgataaag aattcggatc cgccggcgac
     5941 gagaacgtgc gcgtgagcga gaagctggac ttcaacacct tcgagttcga gttcgagaac
     6001 ctgcactacg ccgccggccg ctgccgcacc tacgtgatct tcgacgtgaa gccccagagc
     6061 aagcgcggac gcggcaagcg cctgtggggc tacgtgcgca acaaccccct ggaggaccac
     6121 gccgagatga tcctgatgag caagatcaac caccacctgg ccgccaacaa caaggacaag
     6181 tacaccatga cctggtacat gagctggagc ccctgcggca actgcagcag cgagctggtg
     6241 ccctggctga agatcctgct ggaggagcag cagcacaccc tgaccatgca cttcagccgc
     6301 atctacaaca aggaccgcgc cgtggaccac cgcggcctgc gcgacctgcg cgacctgcag
     6361 cgcgtggtga gcaactactt caagatgggc gtgatgcgcg aaaccgaggt gaagaagtgc
     6421 ctggccgagt acgtggaggc cagccgccgc accctgaagt ggctgcgcac caccgccagc
     6481 aacgccggcc gccgccgccg caagctgagc agcatcctgg tgcgctgcgc cggcatgcgc
     6541 gagagcggca tgcccctgca cctgttcacc gctagaggta cccaattcat gaccaacctt
     6601 tccgacatca tagagaagga aacaggcaaa cagttggtca tccaagagtc gatactcatg
     6661 cttcctgaag aagttgagga ggtcattggg aataagccgg aaagtgacat tctcgtacac
     6721 actgcgtatg atgagagcac cgatgagaac gtgatgctgc tcacgtcaga tgccccagag
     6781 tacaaaccct gggctctggt gattcaggac tctaatggag agaacaagat caagatgcta
     6841 tctggtggtt ctggaggatc tggtggttct caattcayta atctgtcaga tattattgaa
     6901 aaggaaaccg gcaagcaact ggttatccag gaatccatcc tcatgctccc agaggaggtg
     6961 gaagaagtca ttgggaacaa gccggaaagc gatatactcg tgcacaccgc ctacgacgag
     7021 agcaccgacg agaatgtcat gcttctgact agcgacgccc ctgaatacaa gccttgggct
     7081 ctggtcatac aggatagcaa cggtgagaac aagattaaga tgctctctgg tggttctccc
     7141 aagaagaaga ggaaagtcgc tagcggcagt ggagagggca gaggaagtct gctaacatgc
     7201 ggtgacgtcg aggagaatcc tggcccagtg agcaagggcg aggagctgtt caccggggtg
     7261 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     7321 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     7381 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
     7441 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     7501 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     7561 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     7621 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     7681 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     7741 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     7801 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     7861 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     7921 ggcatggacg agctgtacaa ggcaatctaa ctagagctcg ctgatcagcc tcgactgtgc
     7981 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     8041 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     8101 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     8161 agaatagcag gcatgctggg gagcggccgc aggaacccct agtgatggag ttggccactc
     8221 cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg
     8281 gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga
     8341 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc
     8401 atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     8461 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     8521 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     8581 atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag
     8641 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     8701 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcgggct attcttttga
     8761 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     8821 atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac
     8881 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
     8941 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     9001 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
     9061 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     9121 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     9181 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     9241 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     9301 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     9361 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     9421 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     9481 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     9541 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     9601 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     9661 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     9721 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     9781 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     9841 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     9901 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     9961 tggaagccgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
    10021 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
    10081 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
    10141 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
    10201 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
    10261 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
    10321 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
    10381 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
    10441 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
    10501 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
    10561 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
    10621 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
    10681 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
    10741 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
    10801 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
    10861 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
    10921 tcacatgt
//
