LOCUS       sequence-259484-        9833 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing WT TBK1 containing STOP codon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9833)
  AUTHORS   Sarraf SA, Sideris DP, Giagtzoglou N, Ni L, Kankel MW, Sen A, 
            Bochicchio LE, Huang CH, Nussenzweig SC, Worley SH, Morton PD, 
            Artavanis-Tsakonas S, Youle RJ, Pickrell AM
  TITLE     PINK1/Parkin Influences Cell Cycle by Sequestering TBK1 at Damaged 
            Mitochondria, Inhibiting Mitosis.
  JOURNAL   Cell Rep. 2019 Oct 1;29(1):225-235.e5. doi: 
            10.1016/j.celrep.2019.08.085.
  PUBMED    31577952
REFERENCE   2  (bases 1 to 9833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.08"; journalName: "Cell Rep"; date: 
            "2019-10-1- 1"; volume: "29"; issue: "1"; pages: "225-235.e5"
COMMENT     Sequence Label: pHAGE-TBK1-C-FLAG-HA
FEATURES             Location/Qualifiers
     source          1..9833
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     604..623
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        604..622
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    652..676
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2864..2888)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2920..2943
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2959..2985
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             2959..2985
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    2992..3558
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3009..3560
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3153..3170)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3380..3399
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3572..3970
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    3993..4581
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4046..4066)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4464..4475)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4656..4889
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5008..5026)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5094..5198
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5199..5267,5268..6059)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5417..5436)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6230..6818
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6719..6738
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6972..6989
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7229..7250
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7265..7270,7271..7288,7289..7295)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7303..7319
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7308..7330
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7327..7343
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7327..7343
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7393..7412
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7604..8237
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8284..8409
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8906..9139
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9324..9368
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9665..9782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccggcgg ccgcagatct ctcgaggttg
      601 atctaatacg actcactata gggagaccca agctggctag ttaagctatc aacaagtttg
      661 tacaaaaaag ttggcatgca gagcacttct aatcatctgt ggcttttatc tgatatttta
      721 ggccaaggag ctactgcaaa tgtctttcgt ggaagacata agaaaactgg tgatttattt
      781 gctatcaaag tatttaataa cataagcttc cttcgtccag tggatgttca aatgagagaa
      841 tttgaagtgt tgaaaaaact caatcacaaa aatattgtca aattatttgc tattgaagag
      901 gagacaacaa caagacataa agtacttatt atggaatttt gtccatgtgg gagtttatac
      961 actgttttag aagaaccttc taatgcctat ggactaccag aatctgaatt cttaattgtt
     1021 ttgcgagatg tggtgggtgg aatgaatcat ctacgagaga atggtatagt gcaccgtgat
     1081 atcaagccag gaaatatcat gcgtgttata ggggaagatg gacagtctgt gtacaaactc
     1141 acagattttg gtgcagctag agaattagaa gatgatgagc agtttgtttc tctgtatggc
     1201 acagaagaat atttgcaccc tgatatgtat gagagagcag tgctaagaaa agatcatcag
     1261 aagaaatatg gagcaacagt tgatctttgg agcattgggg taacatttta ccatgcagct
     1321 actggatcac tgccatttag accctttgaa gggcctcgta ggaataaaga agtgatgtat
     1381 aaaataatta caggaaagcc ttctggtgca atatctggag tacagaaagc agaaaatgga
     1441 ccaattgact ggagtggaga catgcctgtt tcttgcagtc tttctcgggg tcttcaggtt
     1501 ctacttaccc ctgttcttgc aaacatcctt gaagcagatc aggaaaagtg ttggggtttt
     1561 gaccagtttt ttgcagaaac tagtgatata cttcaccgaa tggtaattca tgttttttcg
     1621 ctacaacaaa tgacagctca taagatttat atacatagct ataatactgc tactatattt
     1681 catgaactgg tatataaaca aaccaaaatt atttcttcaa atcaagaact tatctacgaa
     1741 gggcgacgct tagtcttaga acctggaagg ctggcacaac atttccctaa aactactgag
     1801 gaaaacccta tatttgtagt aagccgggaa cctctgaata ccataggatt aatatatgaa
     1861 aaaatttccc tccctaaagt acatccacgt tatgatttag acggggatgc tagcatggct
     1921 aaggcaataa caggggttgt gtgttatgcc tgcagaattg ccagtacctt actgctttat
     1981 caggaattaa tgcgaaaggg gatacgatgg ctgattgaat taattaaaga tgattacaat
     2041 gaaactgttc acaaaaagac agaagttgtg atcacattgg atttctgtat cagaaacatt
     2101 gaaaaaactg tgaaagtata tgaaaagttg atgaagatca acctggaagc ggcagagtta
     2161 ggtgaaattt cagacataca caccaaattg ttgagacttt ccagttctca gggaacaata
     2221 gaaaccagtc ttcaggatat cgacagcaga ttatctccag gtggatcact ggcagacgca
     2281 tgggcacatc aagaaggcac tcatccgaaa gacagaaatg tagaaaaact acaagtcctg
     2341 ttaaattgca tgacagagat ttactatcag ttcaaaaaag acaaagcaga acgtagatta
     2401 gcttataatg aagaacaaat ccacaaattt gataagcaaa aactgtatta ccatgccaca
     2461 aaagctatga cgcactttac agatgaatgt gttaaaaagt atgaggcatt tttgaataag
     2521 tcagaagaat ggataagaaa gatgcttcat cttaggaaac agttattatc gctgactaat
     2581 cagtgttttg atattgaaga agaagtatca aaatatcaag aatatactaa tgagttacaa
     2641 gaaactctgc ctcagaaaat gtttacagct tccagtggaa tcaaacatac catgacccca
     2701 atttatccaa gttctaacac attagtagaa atgactcttg gtatgaagaa attaaaggaa
     2761 gagatggaag gggtggttaa agaacttgct gaaaataacc acattttaga aaggtttggc
     2821 tctttaacca tggatggtgg ccttcgcaac gttgactgtc tttagccaac tttcttgtac
     2881 aaagtggttg atctagaggg cccgcggttc gaactcgagg actacaagga cgacgatgac
     2941 aagtcggccg ctggaggata cccctacgac gtgcccgact acgcctagaa ttccgccccc
     3001 cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat
     3061 gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt
     3121 cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt
     3181 gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc
     3241 gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc
     3301 acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat
     3361 agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc
     3421 ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg
     3481 tgtttagtcg aggttaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt
     3541 tgaaaaacac gatgataata tggccacaac catggccaag cctttgtctc aagaagaatc
     3601 caccctcatt gaaagagcaa cggctacaat caacagcatc cccatctctg aagactacag
     3661 cgtcgccagc gcagctctct ctagcgacgg ccgcatcttc actggtgtca atgtatatca
     3721 ttttactggg ggaccttgtg cagaactcgt ggtgctgggc actgctgctg ctgcggcagc
     3781 tggcaacctg acttgtatcg tcgcgatcgg aaatgagaac aggggcatct tgagcccctg
     3841 cggacggtgc cgacaggtgc ttctcgatct gcatcctggg atcaaagcca tagtgaagga
     3901 cagtgatgga cagccgacgg cagttgggat tcgtgaattg ctgccctctg gttatgtgtg
     3961 ggagggctaa tgatagctat atattggatc ctaatcaacc tctggattac aaaatttgtg
     4021 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     4081 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     4141 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     4201 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     4261 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     4321 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     4381 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     4441 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     4501 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     4561 ccctttgggc cgcctccccg cctgagatcc tttaagacca atgacttaca aggcagctgt
     4621 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     4681 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     4741 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     4801 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     4861 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     4921 tttataactt gcaaagaaat gaatatcaga gagtgagagg cccgggttaa ttaaggaaag
     4981 ggctagatca ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     5041 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     5101 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     5161 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     5221 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     5281 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     5341 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     5401 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc
     5461 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     5521 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     5581 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     5641 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     5701 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     5761 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     5821 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     5881 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     5941 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     6001 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     6061 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     6121 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     6181 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     6241 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     6301 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     6361 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     6421 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     6481 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     6541 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     6601 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     6661 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     6721 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     6781 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     6841 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     6901 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     6961 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     7021 ctctccccgc gcgttggccg attcattaat gcagcaagct catggctgac taattttttt
     7081 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     7141 cttttttgga ggcctaggct tttgcaaaaa gctccccgtg gcacgacagg tttcccgact
     7201 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     7261 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     7321 ttcacacagg aaacagctat gacatgatta cgaatttcac aaataaagca tttttttcac
     7381 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact
     7441 ggataactca agctaaccaa aatcatccca aacttcccac cccataccct attaccactg
     7501 ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt
     7561 aaagaaattg tatttgttaa atatgtacta caaacttagt agttggaagg gctaattcac
     7621 tcccaaagaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct
     7681 gattagcaga actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc
     7741 tacaagctag taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc
     7801 agcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag
     7861 tggaggtttg acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac
     7921 ttcaagaact gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg
     7981 aggcgtggcc tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct
     8041 gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     8101 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     8161 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     8221 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
     8281 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
     8341 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
     8401 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
     8461 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
     8521 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
     8581 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
     8641 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
     8701 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cggccgctga
     8761 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
     8821 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
     8881 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
     8941 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
     9001 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
     9061 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
     9121 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
     9181 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
     9241 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
     9301 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
     9361 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
     9421 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
     9481 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
     9541 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
     9601 gattagtgaa cggatctcga cggtatcgcc gaattaattc acaaatggca gtattcatcc
     9661 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
     9721 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
     9781 ttcgggttta ttacagggac agcagagatc cagtttggac tagtggagtt ccg
//
