LOCUS       sequence-259501-        1226 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses EGFP-tagged Golgi apparatus marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Schormann W, Hariharan S, Andrews DW
  TITLE     A reference library for assigning protein subcellular localizations 
            by image-based machine learning.
  JOURNAL   J Cell Biol. 2020 Mar 2;219(3). pii: 133635. doi: 
            10.1083/jcb.201904090.
  PUBMED    31968357
REFERENCE   2  (bases 1 to 1226)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2020-03-2"; volume: "219(3). pii: 133635. doi"; 
            pages: " 10.1083/jcb.201904090"
COMMENT     Sequence Label: pLVX-EF1a-EGFP-Membrin-IRES-Puromycin
FEATURES             Location/Qualifiers
     source          1..1226
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     83..103
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      190..199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(196..198,199..201,202..912)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(196..198,199..201,202..912)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(241..262)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(502..521)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     849..870
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gggagggtga gtaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt
       61 gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca
      121 tttcaggtgt cgtgaggatc tatttccggt gaattcctcg agactagttc tagagcggcc
      181 gcaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
      241 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
      301 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
      361 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
      421 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
      481 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
      541 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
      601 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
      661 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
      721 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
      781 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
      841 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
      901 gagctgtaca agtactcaga tctcgacatg gatcccctgt tccagcaaac gcacaagcag
      961 gtccacgaga tccagtcttg catgggacgc ctggagacgg cagacagcag tctgtgcaca
     1021 tagtaaaacg aatccaagca gcatagacca gatattcagc cgtctagacg tctggagatt
     1081 ttgtccagca aggagcccct aacaaggcaa atgcgacttc ggttgacagt agtatgatgt
     1141 ccagcacctg cgactgcctc aaactcagca tcggtcatgc aaggagcaca gtaagaagca
     1201 aagacttcgt tctcgaaacc ttcacc
//
