LOCUS       sequence-259507-        2930 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AausFP1 - bright green fluorescent protein expression from A. 
            australis under CMV promoter in minimal backbone plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2930)
  TITLE     Constitutive promoter minimal backbone plasmids for optimized 
            expression in mammalian cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: CMV-AausFP1-Z1
FEATURES             Location/Qualifiers
     source          1..2930
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        76..379
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        380..583
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     534..554
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    606..686
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      688..697
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    1447..1568
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1458..1477)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1512..1531
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1727..2101)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(2111..2129)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2121..2167)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     rep_origin      complement(2240..2828)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2320..2339)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctgcagaagc tttactaact agtaatagta atcaattacg gggtcattag ttcatagccc
       61 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
      121 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
      181 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
      241 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
      301 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
      361 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
      421 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
      481 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
      541 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca
      601 gatccgctag cgctaccgga ctcagatctc gagctcaagc ttcgaattct gcagtcgacg
      661 gtaccgcggg cccgggatcc accggtcgcc accatgggtt cctatggtgc tctgctgttt
      721 agggagaaga taccttacgt ggtcgaaatg gaaggcgacg tcgaaggcat gaaattcagc
      781 gtgcggggca aggggcacgg agatgccaat acagggaaga tcgaggcttc ctttatatgt
      841 actaccggcg agctgcctgt tccctggagc tctatactca ccaccgtgac ctatggtgcc
      901 caatgcttcg ctaagtaccc gaacgacata aaggattatc ctaagtccgc aatgcccgag
      961 gggtatgtcc aggaacgtac cataacattc gagaatgatg gagtttacaa aacgcgggcc
     1021 gaggttactt atgaaaaggg ctccgtctac aatagggtga cactgaatgg gtccgggttc
     1081 aagaagggcg gaaatattct gggaaagaag ctggagttca attataatcc ccactgtatt
     1141 tacgtcctgc ccgacgttca gaacaacggc attaagtgtt atattaatat cgtgcacgat
     1201 gtgatcggcg gcggtcagat catcgcagcc catcagcagc tgaacacacc actcggcggc
     1261 ggtcccgtag atatcccgca ttatcaccac atccaggccc ataccatcct ctcaaaggac
     1321 ccgaaggaga ccagggacca catgaatgtg gtcgaagtat ttcgtgccat tgactgtaag
     1381 actgcttacg cctgataggc ggccgcgact ctagatgtac acatatggct agctggccag
     1441 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
     1501 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     1561 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
     1621 aggtttttta aagcaagtaa aacctctaca aatgtggtat ggaattctgg actcacaaag
     1681 aaaaaacgcc cggtgtgcaa gaccgagcgt tctgaacaat ttatcctcag tcctgctcct
     1741 ctgccacaaa gtgcacgcag ttgccggccg ggtcgcgcag ggcgaactcc cgcccccacg
     1801 gctgctcgcc gatctcggtc atggccggcc cggaggcgtc ccggaagttc gtggacacga
     1861 cctccgacca ctcggcgtac agctcgtcca ggccgcgcac ccacacccag gccagggtgt
     1921 tgtccggcac cacctggtcc tggaccgcgc tgatgaacag ggtcacgtcg tcccggacca
     1981 caccggcgaa gtcgtcctcc acgaagtccc gggagaaccc gagccggtcg gtccagaact
     2041 cgaccgctcc ggcgacgtcg cgcgcggtga gcaccggaac ggcactggtc aacttggcca
     2101 tgatggccct cctatagtga gtcgtattat actatgccga tatactatgc cgatgattaa
     2161 ttgtcaagga tcctaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     2221 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     2281 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     2341 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     2401 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     2461 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     2521 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     2581 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     2641 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     2701 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     2761 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     2821 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtga aaactcacgt
     2881 taagggattt tggtcatggc tagttaatta acatttaaat cagcggccgc
//
