LOCUS       sequence-25951-e         751 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector for N-term GST fusion, frame 6P-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pENTR4-GST 6P-1 (w487-1)
FEATURES             Location/Qualifiers
     source          1..751
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(88..187)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(299..318)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(377..396)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 cccaacggac tggagttctg ttccaggggc ccctgggatc cccggaattc ccgggtcgac
       61 tcgagcggcc gcactcgaga tatctagacc cagctttctt gtacaaagtt ggcattataa
      121 gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca
      181 ttatttgcca tccagctgca gctctggccc gtgtctcaaa atctctgatg ttacattgca
      241 caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca
      301 aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg
      361 gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca
      421 atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt
      481 agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg
      541 cctcttccga ccatcaagca ttttatccgt actcctggtg atgcatggtt actcaccact
      601 gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat
      661 attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt
      721 ccttttaaca gcgatcgcgg tattttctct c
//
