LOCUS       sequence-259517-       10148 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses human Fzd4 CRD (38-160) in Hi5 insect cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10148)
  AUTHORS   Shen G, Ke J, Wang Z, Cheng Z, Gu X, Wei Y, Melcher K, Xu HE, Xu W
  TITLE     Structural basis of the Norrin-Frizzled 4 interaction.
  JOURNAL   Cell Res. 2015 Sep;25(9):1078-81. doi: 10.1038/cr.2015.92. Epub 2015
            Jul 31.
  PUBMED    26227961
REFERENCE   2  (bases 1 to 10148)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/cr.2015.92"; journalName: "Cell Res"; date: "2015-09"; 
            volume: "25"; issue: "9"; pages: "1078-81"
COMMENT     Sequence Label: pAcGP67B human Fzd4 CRD (38–160)
FEATURES             Location/Qualifiers
     source          1..10148
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     sig_peptide     1..60
                     /codon_start=1
                     /gene="Autographa californica gp64"
                     /product="signal sequence from the baculovirus envelope 
                     glycoprotein GP64"
                     /label=GP64 signal sequence
                     /translation="MVSAIVLYVLLAAAAHSAFA"
     CDS             76..99
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     misc_recomb     968..1673
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     3'UTR           1056..1428
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     CDS             complement(1085..1673)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="IPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNR
                     PNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKAR
                     LLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIF
                     ALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     CDS             complement(2107..2124)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3341..3357)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3341..3357)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3354..3376)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3365..3381
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3389..3395,3396..3413,3414..3419))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3434..3455
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3572..3589)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3743..4331)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3823..3842)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4502..5293,5294..5362))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5125..5144
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5363..5467)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5535..5553
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5591..5613)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5713..5732
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5926..5948
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5940..5957
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5941..5957
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_recomb     9130..9957
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             9130..9254
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(9292..9897)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     promoter        9961..10052
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     9977..9994
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
ORIGIN
        1 atggtaagcg ctattgtttt atatgtgctt ttggcggcgg cggcgcattc tgcctttgcg
       61 gcggatcttg gatcccacca tcaccatcac catcaccatg gggacgagga agagcggcgc
      121 tgcgacccca tccgcatctc catgtgccag aacctcggct acaacgtgac caagatgccc
      181 aacctggttg ggcacgagct gcagacggac gccgagctgc agctgacaac tttcacaccg
      241 ctcatccagt acggctgctc cagccagctg cagttcttcc tttgttctgt ttatgtgcca
      301 atgtgcacag agaagatcaa catccccatt ggcccatgcg gcggcatgtg tctttcagtc
      361 aagagacgct gtgaacccgt cctgaaggaa tttggatttg cctggccaga gagtctgaac
      421 tgcagcaaat tcccaccaca gaacgaccac aaccacatgt gcatggaatg agcggccgct
      481 gcagatctga tcctttcctg ggacccggca agaaccaaaa actcactctc ttcaaggaaa
      541 tccgtaatgt taaacccgac acgatgaagc ttgtcgttgg atggaaagga aaagagttct
      601 acagggaaac ttggacccgc ttcatggaag acagcttccc cattgttaac gaccaagaag
      661 tgatggatgt tttccttgtt gtcaacatgc gtcccactag acccaaccgt tgttacaaat
      721 tcctggccca acacgctctg cgttgcgacc ccgactatgt acctcatgac gtgattagga
      781 tcgtcgagcc ttcatgggtg ggcagcaaca acgagtaccg catcagcctg gctaagaagg
      841 gcggcggctg cccaataatg aaccttcact ctgagtacac caactcgttc gaacagttca
      901 tcgatcgtgt catctgggag aacttctaca agcccatcgt ttacatcggt accgactctg
      961 ctgaagagga ggaaattctc cttgaagttt ccctggtgtt caaagtaaag gagtttgcac
     1021 cagacgcacc tctgttcact ggtccggcgt attaaaacac gatacattgt tattagtaca
     1081 tttattaagc gctagattct gtgcgttgtt gatttacaga caattgttgt acgtatttta
     1141 ataattcatt aaatttataa tctttagggt ggtatgttag agcgaaaatc aaatgatttt
     1201 cagcgtcttt atatctgaat ttaaatatta aatcctcaat agatttgtaa aataggtttc
     1261 gattagtttc aaacaagggt tgtttttccg aaccgatggc tggactatct aatggatttt
     1321 cgctcaacgc cacaaaactt gccaaatctt gtagcagcaa tctagctttg tcgatattcg
     1381 tttgtgtttt gttttgtaat aaaggttcga cgtcgttcaa aatattatgc gcttttgtat
     1441 ttctttcatc actgtcgtta gtgtacaatt gactcgacgt aaacacgtta aataaagctt
     1501 ggacatattt aacatcgggc gtgttagctt tattaggccg attatcgtcg tcgtcccaac
     1561 cctcgtcgtt agaagttgct tccgaagacg attttgccat agccacacga cgcctattaa
     1621 ttgtgtcggc taacacgtcc gcgatcaaat ttgtagttga gctttttgga attatttctg
     1681 attgcgggcg tttttgggcg ggtttcaatc taactgtgcc cgattttaat tcagacaaca
     1741 cgttagaaag cgatggtgca ggcggtggta acatttcaga cggcaaatct actaatggcg
     1801 gcggtggtgg agctgatgat aaatctacca tcggtggagg cgcaggcggg gctggcggcg
     1861 gaggcggagg cggaggtggt ggcggtgatg cagacggcgg tttaggctca aatgtctctt
     1921 taggcaacac agtcggcacc tcaactattg tactggtttc gggcgccgtt tttggtttga
     1981 ccggtctgag acgagtgcga tttttttcgt ttctaatagc ttccaacaat tgttgtctgt
     2041 cgtctaaagg tgcagcgggt tgaggttccg tcggcattgg tggagcgggc ggcaattcag
     2101 acatcgatgg tggtggtggt ggtggaggcg ctggaatgtt aggcacggga gaaggtggtg
     2161 gcggcggtgc cgccggtata atttgttctg gtttagtttg ttcgcgcacg attgtgggca
     2221 ccggcgcagg cgccgctggc tgcacaacgg aaggtcgtct gcttcgaggc agcgcttggg
     2281 gtggtggcaa ttcaatatta taattggaat acaaatcgta aaaatctgct ataagcattg
     2341 taatttcgct atcgtttacc gtgccgatat ttaacaaccg ctcaatgtaa gcaattgtat
     2401 tgtaaagaga ttgtctcaag ctcgcacgcc gataacaagc cttttcattt ttactacagc
     2461 attgtagtgg cgagacactt cgctgtcgtc gacgtacatg tatgctttgt tgtcaaaaac
     2521 gtcgttggca agctttaaaa tatttaaaag aacatctctg ttcagcacca ctgtgttgtc
     2581 gtaaatgttg tttttgataa tttgcgcttc cgcagtatcg acacgttcaa aaaattgatg
     2641 cgcatcaatt ttgttgttcc tattattgaa taaataagat tgtacagatt catatctacg
     2701 attcgtcatg gccaccacaa atgctacgct gcaaacgctg gtacaatttt acgaaaactg
     2761 caaaaacgtc aaaactcggt ataaaataat caacgggcgc tttggcaaaa tatctatttt
     2821 atcgcacaag cccactagca aattgtattt gcagaaaaca atttcggcgc acaattttaa
     2881 cgctgacgaa ataaaagttc accagttaat gagcgaccac ccaaatttta taaaaatcta
     2941 ttttaatcac ggttccatca acaaccaagt gatcgtgatg gactacattg actgtcccga
     3001 tttatttgaa acactacaaa ttaaaggcga gctttcgtac caacttgtta gcaatattat
     3061 tagacagctg tgtgaagcgc tcaacgattt gcacaagcac aatttcatac acaacgacat
     3121 aaaactcgaa aatgtcttat atttcgaagc acttgatcgc gtgtatgttt gcgattacgg
     3181 attgtgcaaa cacgaaaact cacttagcgt gcacgacggc acgttggagt attttagtcc
     3241 ggaaaaaatt cgacaccaca actatgcacg ttcgtttgac tggtacgccg tcggcgtgtt
     3301 aacatacaag ttgctaaccg gcggccaatt cgtaatcatg gtcatagctg tttcctgtgt
     3361 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     3421 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     3481 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     3541 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     3601 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     3661 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     3721 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     3781 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     3841 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     3901 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     3961 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     4021 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     4081 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     4141 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     4201 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     4261 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     4321 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     4381 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     4441 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     4501 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     4561 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     4621 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     4681 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     4741 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     4801 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     4861 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     4921 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     4981 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     5041 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     5101 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     5161 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     5221 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     5281 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     5341 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     5401 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     5461 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     5521 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg
     5581 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     5641 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct
     5701 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
     5761 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc
     5821 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
     5881 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
     5941 gtaaaacgac ggccagtgcc aagcttggct cgtaaagcga gttgaaggat catatttagt
     6001 tgcgtttatg agataagatt gaaagcacgt gtaaaatgtt tcccgcgcgt tggcacaact
     6061 atttacaatg cggccaagtt ataaaagatt ctaatctgat atgttttaaa acacctttgc
     6121 ggcccgagtt gtttgcgtac gtgactagcg aagaagatat gtggaccgca gaacagatag
     6181 taaaacaaaa ccctagtatt ggagcaataa tcgatttaac caacacgtct aaatattatg
     6241 atggtgtgca ttttttgcgg gcgggcctgt tatacaaaaa aattcaagta cctggccaga
     6301 ctttgccgcc tgaaagcata gttcaagaat ttattgacac ggtaaaagaa tttacagaaa
     6361 agtgtcccgg catgttggtg ggcgtgcact gcacacacgg tattaatcgc accggttaca
     6421 tggtgtgcag atatttaatg cacaccctgg gtattgcgcc gcaggaagcc atagatagat
     6481 tcgaaaaagc cagaggtcac aaaattgaaa gacaaaatta cgttcaagat ttattaattt
     6541 aattaatatt atttgcattc tttaacaaat actttatcct attttcaaat tgttgcgctt
     6601 cttccagcga accaaaacta tgcttcgctt gctccgttta gcttgtagcc gatcagtggc
     6661 gttgttccaa tcgacggtag gattaggccg gatattctcc accacaatgt tggcaacgtt
     6721 gatgttacgt ttatgctttt ggttttccac gtacgtcttt tggccggtaa tagccgtaaa
     6781 cgtagtgccg tcgcgcgtca cgcacaacac cggatgtttg cgcttgtccg cggggtattg
     6841 aaccgcgcga tccgacaaat ccaccacttt ggcaactaaa tcggtgacct gcgcgtcttt
     6901 tttctgcatt atttcgtctt tcttttgcat ggtttcctgg aagccggtgt acatgcggtt
     6961 tagatcagtc atgacgcgcg tgacctgcaa atctttggcc tcgatctgct tgtccttgat
     7021 ggcaacgatg cgttcaataa actcttgttt tttaacaagt tcctcggttt tttgcgccac
     7081 caccgcttgc agcgcgtttg tgtgctcggt gaatgtcgca atcagcttag tcaccaactg
     7141 tttgctctcc tcctcccgtt gtttgatcgc gggatcgtac ttgccggtgc agagcacttg
     7201 aggaattact tcttctaaaa gccattcttg taattctatg gcgtaaggca atttggactt
     7261 cataatcagc tgaatcacgc cggatttagt aatgagcact gtatgcggct gcaaatacag
     7321 cgggtcgccc cttttcacga cgctgttaga ggtagggccc ccattttgga tggtctgctc
     7381 aaataacgat ttgtatttat tgtctacatg aacacgtata gctttatcac aaactgtata
     7441 ttttaaactg ttagcgacgt ccttggccac gaaccggacc tgttggtcgc gctctagcac
     7501 gtaccgcagg ttgaacgtat cttctccaaa tttaaattct ccaattttaa cgcgagccat
     7561 tttgatacac gtgtgtcgat tttgcaacaa ctattgtttt ttaacgcaaa ctaaacttat
     7621 tgtggtaagc aataattaaa tatgggggaa catgcgccgc tacaacactc gtcgttatga
     7681 acgcagacgg cgccggtctc ggcgcaagcg gctaaaacgt gttgcgcgtt caacgcggca
     7741 aacatcgcaa aagccaatag tacagttttg atttgcatat taacggcgat tttttaaatt
     7801 atcttattta ataaatagtt atgacgccta caactccccg cccgcgttga ctcgctgcac
     7861 ctcgagcagt tcgttgacgc cttcctccgt gtggccgaac acgtcgagcg ggtggtcgat
     7921 gaccagcggc gtgccgcacg cgacgcacaa gtatctgtac accgaatgat cgtcgggcga
     7981 aggcacgtcg gcctccaagt ggcaatattg gcaaattcga aaatatatac agttgggttg
     8041 tttgcgcata tctatcgtgg cgttgggcat gtacgtccga acgttgattt gcatgcaagc
     8101 cgaaattaaa tcattgcgat tagtgcgatt aaaacgttgt acatcctcgc ttttaatcat
     8161 gccgtcgatt aaatcgcgca atcgagtcaa gtgatcaaag tgtggaataa tgttttcttt
     8221 gtattcccga gtcaagcgca gcgcgtattt taacaaacta gccatcttgt aagttagttt
     8281 catttaatgc aactttatcc aataatatat tatgtatcgc acgtcaagaa ttaacaatgc
     8341 gcccgttgtc gcatctcaac acgactatga tagagatcaa ataaagcgcg aattaaatag
     8401 cttgcgacgc aacgtgcacg atctgtgcac gcgttccggc acgagctttg attgtaataa
     8461 gtttttacga agcgatgaca tgacccccgt agtgacaacg atcacgccca aaagaactgc
     8521 cgactacaaa attaccgagt atgtcggtga cgttaaaact attaagccat ccaatcgacc
     8581 gttagtcgaa tcaggaccgc tggtgcgaga agccgcgaag tatggcgaat gcatcgtata
     8641 acgtgtggag tccgctcatt agagcgtcat gtttagacaa gaaagctaca tatttaattg
     8701 atcccgatga ttttattgat aaattgaccc taactccata cacggtattc tacaatggcg
     8761 gggttttggt caaaatttcc ggactgcgat tgtacatgct gttaacggct ccgcccacta
     8821 ttaatgaaat taaaaattcc aattttaaaa aacgcagcaa gagaaacatt tgtatgaaag
     8881 aatgcgtaga aggaaagaaa aatgtcgtcg acatgctgaa caacaagatt aatatgcctc
     8941 cgtgtataaa aaaaatattg aacgatttga aagaaaacaa tgtaccgcgc ggcggtatgt
     9001 acaggaagag gtttatacta aactgttaca ttgcaaacgt ggtttcgtgt gccaagtgtg
     9061 aaaaccgatg tttaatcaag gctctgacgc atttctacaa ccacgactcc aagtgtgtgg
     9121 gtgaagtcat gcatctttta atcaaatccc aagatgtgta taaaccacca aactgccaaa
     9181 aaatgaaaac tgtcgacaag ctctgtccgt ttgctggcaa ctgcaagggt ctcaatccta
     9241 tttgtaatta ttgaataata aaacaattat aaatgtcaaa tttgtttttt attaacgata
     9301 caaaccaaac gcaacaagaa catttgtagt attatctata attgaaaacg cgtagttata
     9361 atcgctgagg taatatttaa aatcattttc aaatgattca cagttaattt gcgacaatat
     9421 aattttattt tcacataaac tagacgcctt gtcgtcttct tcttcgtatt ccttctcttt
     9481 ttcatttttc tcctcataaa aattaacata gttattatcg tatccatata tgtatctatc
     9541 gtatagagta aattttttgt tgtcataaat atatatgtct tttttaatgg ggtgtatagt
     9601 accgctgcgc atagtttttc tgtaatttac aacagtgcta ttttctggta gttcttcgga
     9661 gtgtgttgct ttaattatta aatttatata atcaatgaat ttgggatcgt cggttttgta
     9721 caatatgttg ccggcatagt acgcagcttc ttctagttca attacaccat tttttagcag
     9781 caccggatta acataacttt ccaaaatgtt gtacgaaccg ttaaacaaaa acagttcacc
     9841 tcccttttct atactattgt ctgcgagcag ttgtttgttg ttaaaaataa cagccattgt
     9901 aatgagacgc acaaactaat atcacaaact ggaaatgtct atcaatatat agttgctgat
     9961 atcatggaga taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc
    10021 gtaacagttt tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca
    10081 tcgggcgcgg atctatgcta ctagtaaatc agtcacacca aggcttcaat aaggaacaca
    10141 caagcaag
//
