LOCUS       sequence-25952-e         582 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral Gateway destination vector, expression, PGK 
            promoter, Blast .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 582)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pLenti PGK Blast DEST (w524-1)
FEATURES             Location/Qualifiers
     source          1..582
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      33..168
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    208..329
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(219..238)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     273..292
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             401..581
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 actcactata gggcgaattg ggtacgtccc taggcctcca aaaaagcctc ctcactactt
       61 ctggaatagc tcagaggccg aggcggcctc ggcctctgca taaataaaaa aaattagtca
      121 gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag
      181 gggcgggata gctagagcca gacatgataa gatacattga tgagtttgga caaaccacaa
      241 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
      301 taaccattat aagctgcaat aaacaagttc ctctcactct ctgatattca tttctttgca
      361 agttataaat actgaataat aagatgacat gaactactac tgctagagat tttccacact
      421 gactaaaagg gtctgaggga tctctagtta ccagagtcac acaacagacg ggcacacact
      481 acttgaagca ctcaaggcaa gctttattga ggcttaagca gtgggttccc tagttagcca
      541 gagagctccc aggctcagat ctggtctaac cagagagacc ca
//
