LOCUS       sequence-259532-       11602 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses human LRP6 E1E2 (20–629) in Hi5 insect cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11602)
  AUTHORS   Cheng Z, Biechele T, Wei Z, Morrone S, Moon RT, Wang L, Xu W
  TITLE     Crystal structures of the extracellular domain of LRP6 and its 
            complex with DKK1.
  JOURNAL   Nat Struct Mol Biol. 2011 Oct 9;18(11):1204-10. doi: 
            10.1038/nsmb.2139.
  PUBMED    21984209
REFERENCE   2  (bases 1 to 11602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb"; 
            journalName: "Nat Struct Mol Biol"; date: "2011-10-9- 9"; volume: 
            "18"; issue: "11"; pages: "1204-10"
COMMENT     Sequence Label: pAcGP67A human LRP6 E1E2 (20–629)
FEATURES             Location/Qualifiers
     source          1..11602
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     sig_peptide     1..60
                     /codon_start=1
                     /gene="Autographa californica gp64"
                     /product="signal sequence from the baculovirus envelope 
                     glycoprotein GP64"
                     /label=GP64 signal sequence
                     /translation="MVSAIVLYVLLAAAAHSAFA"
     CDS             70..93
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     misc_recomb     2422..3127
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     3'UTR           2510..2882
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     CDS             complement(2539..3127)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="IPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNR
                     PNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKAR
                     LLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIF
                     ALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     CDS             complement(3561..3578)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4795..4811)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4795..4811)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4808..4830)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4819..4835
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4843..4849,4850..4867,4868..4873))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4888..4909
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5026..5043)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5197..5785)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5277..5296)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5956..6747,6748..6816))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6579..6598
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6817..6921)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6989..7007
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7045..7067)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7167..7186
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7380..7402
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7394..7411
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7395..7411
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_recomb     10584..11411
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             10584..10708
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(10746..11351)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     promoter        11415..11506
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     11431..11448
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
ORIGIN
        1 atggtaagcg ctattgtttt atatgtgctt ttggcggcgg cggcgcattc tgcctttgcg
       61 gcggatccac accatcacca tcaccatcac catgcccctt tgttgcttta tgcaaacaga
      121 cgggacttgc gattggttga tgctacaaat ggcaaagaga atgctacgat tgtagttgga
      181 ggcttggagg atgcagctgc ggtggacttt gtgtttagtc atggcttgat atactggagt
      241 gatgtcagcg aagaagccat taaacgaaca gaatttaaca aaactgagag tgtgcagaat
      301 gttgttgttt ctggattatt gtcccccgat gggctggcat gtgattggct tggagaaaaa
      361 ttgtactgga cagattctga aactaatcgg attgaagttt ctaatttaga tggatcttta
      421 cgaaaagttt tattttggca agagttggat caacccagag ctattgcctt agatccttca
      481 agtgggttca tgtactggac agactgggga gaagtgccaa agatagaacg tgctggaatg
      541 gatggttcaa gtcgcttcat tataataaac agtgaaattt actggccaaa tggactgact
      601 ttggattatg aagaacaaaa gctttattgg gcagatgcaa aacttaattt catccacaaa
      661 tcaaatctgg atggaacaaa tcggcaggca gtggttaaag gttcccttcc acatcctttt
      721 gccttgacgt tatttgagga catattgtac tggactgact ggagcacaca ctccattttg
      781 gcttgcaaca agtatactgg tgagggtctg cgtgaaatcc attctgacat cttctctccc
      841 atggatatac atgccttcag ccaacagagg cagccaaatg ccacaaatcc atgtggaatt
      901 gacaatgggg gttgttccca tttgtgtttg atgtctccag tcaagccttt ttatcagtgt
      961 gcttgcccca ctggggtcaa actcctggag aatggaaaaa cctgcaaaga tggtgccaca
     1021 gaattattgc ttttagctcg aaggacagac ttgagacgca tttctttgga tacaccagat
     1081 tttacagaca ttgttctgca gttagaagac atccgtcatg ccattgccat agattacgat
     1141 cctgtggaag gctacatcta ctggactgat gatgaagtga gggccatacg ccgttcattt
     1201 atagatggat ctggcagtca gtttgtggtc actgctcaaa ttgcccatcc tgatggtatt
     1261 gctgtggact gggttgcacg aaatctttat tggacagaca ctggcactga tcgaatagaa
     1321 gtgacaaggc tcaatgggac catgaggaag atcttgattt cagaggactt agaggaaccc
     1381 cgggctattg tgttagatcc catggttggg tacatgtatt ggactgactg gggagaaatt
     1441 ccgaaaattg agcgagcagc tctggatggt tctgaccgtg tagtattggt taacacttct
     1501 cttggttggc caaatggttt agccttggat tatgatgaag gcaaaatata ctggggagat
     1561 gccaaaacag acaagattga ggttatgaat actgatggca ctgggagacg agtactagtg
     1621 gaagacaaaa ttcctcacat atttggattt actttgttgg gtgactatgt ttactggact
     1681 gactggcaga ggcgtagcat tgaaagagtt cataaacgaa gtgcagagag ggaagtgatc
     1741 atagatcagc tgcctgacct catgggccta aaggctacaa atgttcatcg agtgattggt
     1801 tccaacccct gtgctgagga aaacggggga tgtagccatc tctgcctcta tagacctcag
     1861 ggccttcgct gtgcttgccc tattggcttt gaactcatca gtgacatgaa gacctgcatt
     1921 gtctagcggc cgctgcagat ctgatccttt cctgggaccc ggcaagaacc aaaaactcac
     1981 tctcttcaag gaaatccgta atgttaaacc cgacacgatg aagcttgtcg ttggatggaa
     2041 aggaaaagag ttctacaggg aaacttggac ccgcttcatg gaagacagct tccccattgt
     2101 taacgaccaa gaagtgatgg atgttttcct tgttgtcaac atgcgtccca ctagacccaa
     2161 ccgttgttac aaattcctgg cccaacacgc tctgcgttgc gaccccgact atgtacctca
     2221 tgacgtgatt aggatcgtcg agccttcatg ggtgggcagc aacaacgagt accgcatcag
     2281 cctggctaag aagggcggcg gctgcccaat aatgaacctt cactctgagt acaccaactc
     2341 gttcgaacag ttcatcgatc gtgtcatctg ggagaacttc tacaagccca tcgtttacat
     2401 cggtaccgac tctgctgaag aggaggaaat tctccttgaa gtttccctgg tgttcaaagt
     2461 aaaggagttt gcaccagacg cacctctgtt cactggtccg gcgtattaaa acacgataca
     2521 ttgttattag tacatttatt aagcgctaga ttctgtgcgt tgttgattta cagacaattg
     2581 ttgtacgtat tttaataatt cattaaattt ataatcttta gggtggtatg ttagagcgaa
     2641 aatcaaatga ttttcagcgt ctttatatct gaatttaaat attaaatcct caatagattt
     2701 gtaaaatagg tttcgattag tttcaaacaa gggttgtttt tccgaaccga tggctggact
     2761 atctaatgga ttttcgctca acgccacaaa acttgccaaa tcttgtagca gcaatctagc
     2821 tttgtcgata ttcgtttgtg ttttgttttg taataaaggt tcgacgtcgt tcaaaatatt
     2881 atgcgctttt gtatttcttt catcactgtc gttagtgtac aattgactcg acgtaaacac
     2941 gttaaataaa gcttggacat atttaacatc gggcgtgtta gctttattag gccgattatc
     3001 gtcgtcgtcc caaccctcgt cgttagaagt tgcttccgaa gacgattttg ccatagccac
     3061 acgacgccta ttaattgtgt cggctaacac gtccgcgatc aaatttgtag ttgagctttt
     3121 tggaattatt tctgattgcg ggcgtttttg ggcgggtttc aatctaactg tgcccgattt
     3181 taattcagac aacacgttag aaagcgatgg tgcaggcggt ggtaacattt cagacggcaa
     3241 atctactaat ggcggcggtg gtggagctga tgataaatct accatcggtg gaggcgcagg
     3301 cggggctggc ggcggaggcg gaggcggagg tggtggcggt gatgcagacg gcggtttagg
     3361 ctcaaatgtc tctttaggca acacagtcgg cacctcaact attgtactgg tttcgggcgc
     3421 cgtttttggt ttgaccggtc tgagacgagt gcgatttttt tcgtttctaa tagcttccaa
     3481 caattgttgt ctgtcgtcta aaggtgcagc gggttgaggt tccgtcggca ttggtggagc
     3541 gggcggcaat tcagacatcg atggtggtgg tggtggtgga ggcgctggaa tgttaggcac
     3601 gggagaaggt ggtggcggcg gtgccgccgg tataatttgt tctggtttag tttgttcgcg
     3661 cacgattgtg ggcaccggcg caggcgccgc tggctgcaca acggaaggtc gtctgcttcg
     3721 aggcagcgct tggggtggtg gcaattcaat attataattg gaatacaaat cgtaaaaatc
     3781 tgctataagc attgtaattt cgctatcgtt taccgtgccg atatttaaca accgctcaat
     3841 gtaagcaatt gtattgtaaa gagattgtct caagctcgca cgccgataac aagccttttc
     3901 atttttacta cagcattgta gtggcgagac acttcgctgt cgtcgacgta catgtatgct
     3961 ttgttgtcaa aaacgtcgtt ggcaagcttt aaaatattta aaagaacatc tctgttcagc
     4021 accactgtgt tgtcgtaaat gttgtttttg ataatttgcg cttccgcagt atcgacacgt
     4081 tcaaaaaatt gatgcgcatc aattttgttg ttcctattat tgaataaata agattgtaca
     4141 gattcatatc tacgattcgt catggccacc acaaatgcta cgctgcaaac gctggtacaa
     4201 ttttacgaaa actgcaaaaa cgtcaaaact cggtataaaa taatcaacgg gcgctttggc
     4261 aaaatatcta ttttatcgca caagcccact agcaaattgt atttgcagaa aacaatttcg
     4321 gcgcacaatt ttaacgctga cgaaataaaa gttcaccagt taatgagcga ccacccaaat
     4381 tttataaaaa tctattttaa tcacggttcc atcaacaacc aagtgatcgt gatggactac
     4441 attgactgtc ccgatttatt tgaaacacta caaattaaag gcgagctttc gtaccaactt
     4501 gttagcaata ttattagaca gctgtgtgaa gcgctcaacg atttgcacaa gcacaatttc
     4561 atacacaacg acataaaact cgaaaatgtc ttatatttcg aagcacttga tcgcgtgtat
     4621 gtttgcgatt acggattgtg caaacacgaa aactcactta gcgtgcacga cggcacgttg
     4681 gagtatttta gtccggaaaa aattcgacac cacaactatg cacgttcgtt tgactggtac
     4741 gccgtcggcg tgttaacata caagttgcta accggcggcc aattcgtaat catggtcata
     4801 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     4861 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     4921 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     4981 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     5041 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     5101 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     5161 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     5221 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     5281 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     5341 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     5401 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     5461 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     5521 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     5581 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     5641 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     5701 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     5761 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     5821 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     5881 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     5941 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     6001 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     6061 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     6121 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     6181 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     6241 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     6301 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     6361 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     6421 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     6481 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     6541 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     6601 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     6661 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     6721 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     6781 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     6841 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     6901 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     6961 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
     7021 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     7081 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     7141 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
     7201 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
     7261 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     7321 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
     7381 ccagtcacga cgttgtaaaa cgacggccag tgccaagctt ggctcgtaaa gcgagttgaa
     7441 ggatcatatt tagttgcgtt tatgagataa gattgaaagc acgtgtaaaa tgtttcccgc
     7501 gcgttggcac aactatttac aatgcggcca agttataaaa gattctaatc tgatatgttt
     7561 taaaacacct ttgcggcccg agttgtttgc gtacgtgact agcgaagaag atatgtggac
     7621 cgcagaacag atagtaaaac aaaaccctag tattggagca ataatcgatt taaccaacac
     7681 gtctaaatat tatgatggtg tgcatttttt gcgggcgggc ctgttataca aaaaaattca
     7741 agtacctggc cagactttgc cgcctgaaag catagttcaa gaatttattg acacggtaaa
     7801 agaatttaca gaaaagtgtc ccggcatgtt ggtgggcgtg cactgcacac acggtattaa
     7861 tcgcaccggt tacatggtgt gcagatattt aatgcacacc ctgggtattg cgccgcagga
     7921 agccatagat agattcgaaa aagccagagg tcacaaaatt gaaagacaaa attacgttca
     7981 agatttatta atttaattaa tattatttgc attctttaac aaatacttta tcctattttc
     8041 aaattgttgc gcttcttcca gcgaaccaaa actatgcttc gcttgctccg tttagcttgt
     8101 agccgatcag tggcgttgtt ccaatcgacg gtaggattag gccggatatt ctccaccaca
     8161 atgttggcaa cgttgatgtt acgtttatgc ttttggtttt ccacgtacgt cttttggccg
     8221 gtaatagccg taaacgtagt gccgtcgcgc gtcacgcaca acaccggatg tttgcgcttg
     8281 tccgcggggt attgaaccgc gcgatccgac aaatccacca ctttggcaac taaatcggtg
     8341 acctgcgcgt cttttttctg cattatttcg tctttctttt gcatggtttc ctggaagccg
     8401 gtgtacatgc ggtttagatc agtcatgacg cgcgtgacct gcaaatcttt ggcctcgatc
     8461 tgcttgtcct tgatggcaac gatgcgttca ataaactctt gttttttaac aagttcctcg
     8521 gttttttgcg ccaccaccgc ttgcagcgcg tttgtgtgct cggtgaatgt cgcaatcagc
     8581 ttagtcacca actgtttgct ctcctcctcc cgttgtttga tcgcgggatc gtacttgccg
     8641 gtgcagagca cttgaggaat tacttcttct aaaagccatt cttgtaattc tatggcgtaa
     8701 ggcaatttgg acttcataat cagctgaatc acgccggatt tagtaatgag cactgtatgc
     8761 ggctgcaaat acagcgggtc gccccttttc acgacgctgt tagaggtagg gcccccattt
     8821 tggatggtct gctcaaataa cgatttgtat ttattgtcta catgaacacg tatagcttta
     8881 tcacaaactg tatattttaa actgttagcg acgtccttgg ccacgaaccg gacctgttgg
     8941 tcgcgctcta gcacgtaccg caggttgaac gtatcttctc caaatttaaa ttctccaatt
     9001 ttaacgcgag ccattttgat acacgtgtgt cgattttgca acaactattg ttttttaacg
     9061 caaactaaac ttattgtggt aagcaataat taaatatggg ggaacatgcg ccgctacaac
     9121 actcgtcgtt atgaacgcag acggcgccgg tctcggcgca agcggctaaa acgtgttgcg
     9181 cgttcaacgc ggcaaacatc gcaaaagcca atagtacagt tttgatttgc atattaacgg
     9241 cgatttttta aattatctta tttaataaat agttatgacg cctacaactc cccgcccgcg
     9301 ttgactcgct gcacctcgag cagttcgttg acgccttcct ccgtgtggcc gaacacgtcg
     9361 agcgggtggt cgatgaccag cggcgtgccg cacgcgacgc acaagtatct gtacaccgaa
     9421 tgatcgtcgg gcgaaggcac gtcggcctcc aagtggcaat attggcaaat tcgaaaatat
     9481 atacagttgg gttgtttgcg catatctatc gtggcgttgg gcatgtacgt ccgaacgttg
     9541 atttgcatgc aagccgaaat taaatcattg cgattagtgc gattaaaacg ttgtacatcc
     9601 tcgcttttaa tcatgccgtc gattaaatcg cgcaatcgag tcaagtgatc aaagtgtgga
     9661 ataatgtttt ctttgtattc ccgagtcaag cgcagcgcgt attttaacaa actagccatc
     9721 ttgtaagtta gtttcattta atgcaacttt atccaataat atattatgta tcgcacgtca
     9781 agaattaaca atgcgcccgt tgtcgcatct caacacgact atgatagaga tcaaataaag
     9841 cgcgaattaa atagcttgcg acgcaacgtg cacgatctgt gcacgcgttc cggcacgagc
     9901 tttgattgta ataagttttt acgaagcgat gacatgaccc ccgtagtgac aacgatcacg
     9961 cccaaaagaa ctgccgacta caaaattacc gagtatgtcg gtgacgttaa aactattaag
    10021 ccatccaatc gaccgttagt cgaatcagga ccgctggtgc gagaagccgc gaagtatggc
    10081 gaatgcatcg tataacgtgt ggagtccgct cattagagcg tcatgtttag acaagaaagc
    10141 tacatattta attgatcccg atgattttat tgataaattg accctaactc catacacggt
    10201 attctacaat ggcggggttt tggtcaaaat ttccggactg cgattgtaca tgctgttaac
    10261 ggctccgccc actattaatg aaattaaaaa ttccaatttt aaaaaacgca gcaagagaaa
    10321 catttgtatg aaagaatgcg tagaaggaaa gaaaaatgtc gtcgacatgc tgaacaacaa
    10381 gattaatatg cctccgtgta taaaaaaaat attgaacgat ttgaaagaaa acaatgtacc
    10441 gcgcggcggt atgtacagga agaggtttat actaaactgt tacattgcaa acgtggtttc
    10501 gtgtgccaag tgtgaaaacc gatgtttaat caaggctctg acgcatttct acaaccacga
    10561 ctccaagtgt gtgggtgaag tcatgcatct tttaatcaaa tcccaagatg tgtataaacc
    10621 accaaactgc caaaaaatga aaactgtcga caagctctgt ccgtttgctg gcaactgcaa
    10681 gggtctcaat cctatttgta attattgaat aataaaacaa ttataaatgt caaatttgtt
    10741 ttttattaac gatacaaacc aaacgcaaca agaacatttg tagtattatc tataattgaa
    10801 aacgcgtagt tataatcgct gaggtaatat ttaaaatcat tttcaaatga ttcacagtta
    10861 atttgcgaca atataatttt attttcacat aaactagacg ccttgtcgtc ttcttcttcg
    10921 tattccttct ctttttcatt tttctcctca taaaaattaa catagttatt atcgtatcca
    10981 tatatgtatc tatcgtatag agtaaatttt ttgttgtcat aaatatatat gtctttttta
    11041 atggggtgta tagtaccgct gcgcatagtt tttctgtaat ttacaacagt gctattttct
    11101 ggtagttctt cggagtgtgt tgctttaatt attaaattta tataatcaat gaatttggga
    11161 tcgtcggttt tgtacaatat gttgccggca tagtacgcag cttcttctag ttcaattaca
    11221 ccatttttta gcagcaccgg attaacataa ctttccaaaa tgttgtacga accgttaaac
    11281 aaaaacagtt cacctccctt ttctatacta ttgtctgcga gcagttgttt gttgttaaaa
    11341 ataacagcca ttgtaatgag acgcacaaac taatatcaca aactggaaat gtctatcaat
    11401 atatagttgc tgatatcatg gagataatta aaatgataac catctcgcaa ataaataagt
    11461 attttactgt tttcgtaaca gttttgtaat aaaaaaacct ataaatattc cggattattc
    11521 ataccgtccc accatcgggc gcggatctat gctactagta aatcagtcac accaaggctt
    11581 caataaggaa cacacaagca ag
//
