LOCUS       sequence-259533-        9650 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector plasmid expressing ABEmax and sgRNA scaffold with MS2 
            replacing the Tetraloop and loop 2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9650)
  AUTHORS   Lyu P, Lu Z, Cho SI, Yadav M, Yoo KW, Atala A, Kim JS, Lu B
  TITLE     Adenine Base Editor Ribonucleoproteins Delivered by Lentivirus-Like 
            Particles Show High On-Target Base Editing and Undetectable RNA 
            Off-Target Activities.
  JOURNAL   CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1089/crispr.2020.0095.
  PUBMED    33616436
REFERENCE   2  (bases 1 to 9650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/crispr.2020"; journalName: "CRISPR J"; date: "2021-02"; 
            volume: "4"; issue: "1"; pages: "69-81"
COMMENT     Sequence Label: pspCas9-ABE-3'UTR-sgRNA-2xMS2
FEATURES             Location/Qualifiers
     source          1..9650
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   179..319
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   179..308
                     /label=AAV2 ITR
     rep_origin      394..849
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(481..500)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     691..712
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(866..885)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     985..1007
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1045..1063)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1131..1235
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1236..1304,1305..2096)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1454..1473)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2267..2855
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2756..2775
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2917..3165
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2917..2937
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     3088..3107
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        3206..3224
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        3276..3294
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     enhancer        3338..3623
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        3624..3827
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3778..3798
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3869..3888
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3869..3887
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3937..3957
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(5146..5169,5170..5172,5173..9246)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5146..9246
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             9247..9294
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(9604..9621)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    join(9610..9650,1..167)
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
       61 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
      121 gacagcaagg gggaggattg ggaagagaat agcaggcatg ctggggaggt acggccgcag
      181 gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc
      241 gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga
      301 gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc tccttacgca tctgtgcggt
      361 atttcacacc gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg
      421 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg
      481 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
      541 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
      601 aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
      661 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
      721 tcaactctat ctcgggctat tcttttgatt tataagggat tttgccgatt tcggtctatt
      781 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt
      841 ttacaatttt atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
      901 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
      961 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     1021 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     1081 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     1141 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     1201 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     1261 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     1321 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     1381 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     1441 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     1501 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     1561 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     1621 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     1681 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     1741 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     1801 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     1861 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     1921 ttgctgataa atctggagcc ggtgagcgtg gaagccgcgg tatcattgca gcactggggc
     1981 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     2041 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     2101 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     2161 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     2221 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     2281 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     2341 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     2401 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     2461 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     2521 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     2581 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     2641 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     2701 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     2761 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     2821 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     2881 ggttcctggc cttttgctgg ccttttgctc acatgtgagg gcctatttcc catgattcct
     2941 tcatatttgc atatacgata caaggctgtt agagagataa ttggaattaa tttgactgta
     3001 aacacaaaga tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt
     3061 gcagttttaa aattatgttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat
     3121 ttcgatttct tggctttata tatcttgtgg aaaggacgaa acaccggtgt cttcctcgag
     3181 gaagacccgt ttgagagcta ggccaacatg aggatcaccc atgtctgcag ggcctagcaa
     3241 gttcaaataa ggctagtccg ttatcaactt ggccaacatg aggatcaccc atgtctgcag
     3301 ggccaagtgg caccgagtcg gtgctttttt tggtacccgt tacataactt acggtaaatg
     3361 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaatagta acgccaatag
     3421 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     3481 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     3541 cctggcattg tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     3601 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
     3661 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     3721 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     3781 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctggt ttagtgaacc
     3841 gtcagatccg ctagagatcc gcggccgcta atacgactca ctatagggag agccgccacc
     3901 atgaaacgga cagccgacgg aagcgagttc gagtcaccaa agaagaagcg gaaagtctct
     3961 gaagtcgagt ttagccacga gtattggatg aggcacgcac tgaccctggc aaagcgagca
     4021 tgggatgaaa gagaagtccc cgtgggcgcc gtgctggtgc acaacaatag agtgatcgga
     4081 gagggatgga acaggccaat cggccgccac gaccctaccg cacacgcaga gatcatggca
     4141 ctgaggcagg gaggcctggt catgcagaat taccgcctga tcgatgccac cctgtatgtg
     4201 acactggagc catgcgtgat gtgcgcagga gcaatgatcc acagcaggat cggaagagtg
     4261 gtgttcggag cacgggacgc caagaccggc gcagcaggct ccctgatgga tgtgctgcac
     4321 caccccggca tgaaccaccg ggtggagatc acagagggaa tcctggcaga cgagtgcgcc
     4381 gccctgctga gcgatttctt tagaatgcgg agacaggaga tcaaggccca gaagaaggca
     4441 cagagctcca ccgactctgg aggatctagc ggaggatcct ctggaagcga gacaccaggc
     4501 acaagcgagt ccgccacacc agagagctcc ggcggctcct ccggaggatc ctctgaggtg
     4561 gagttttccc acgagtactg gatgagacat gccctgaccc tggccaagag ggcacgcgat
     4621 gagagggagg tgcctgtggg agccgtgctg gtgctgaaca atagagtgat cggcgagggc
     4681 tggaacagag ccatcggcct gcacgaccca acagcccatg ccgaaattat ggccctgaga
     4741 cagggcggcc tggtcatgca gaactacaga ctgattgacg ccaccctgta cgtgacattc
     4801 gagccttgcg tgatgtgcgc cggcgccatg atccactcta ggatcggccg cgtggtgttt
     4861 ggcgtgagga acgcaaaaac cggcgccgca ggctccctga tggacgtgct gcactacccc
     4921 ggcatgaatc accgcgtcga aattaccgag ggaatcctgg cagatgaatg tgccgccctg
     4981 ctgtgctatt tctttcggat gcctagacag gtgttcaatg ctcagaagaa ggcccagagc
     5041 tccaccgact ccggaggatc tagcggaggc tcctctggct ctgagacacc tggcacaagc
     5101 gagagcgcaa cacctgaaag cagcgggggc agcagcgggg ggtcagacaa gaagtacagc
     5161 atcggcctgg ccatcggcac caactctgtg ggctgggccg tgatcaccga cgagtacaag
     5221 gtgcccagca agaaattcaa ggtgctgggc aacaccgacc ggcacagcat caagaagaac
     5281 ctgatcggag ccctgctgtt cgacagcggc gaaacagccg aggccacccg gctgaagaga
     5341 accgccagaa gaagatacac cagacggaag aaccggatct gctatctgca agagatcttc
     5401 agcaacgaga tggccaaggt ggacgacagc ttcttccaca gactggaaga gtccttcctg
     5461 gtggaagagg ataagaagca cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg
     5521 gcctaccacg agaagtaccc caccatctac cacctgagaa agaaactggt ggacagcacc
     5581 gacaaggccg acctgcggct gatctatctg gccctggccc acatgatcaa gttccggggc
     5641 cacttcctga tcgagggcga cctgaacccc gacaacagcg acgtggacaa gctgttcatc
     5701 cagctggtgc agacctacaa ccagctgttc gaggaaaacc ccatcaacgc cagcggcgtg
     5761 gacgccaagg ccatcctgtc tgccagactg agcaagagca gacggctgga aaatctgatc
     5821 gcccagctgc ccggcgagaa gaagaatggc ctgttcggaa acctgattgc cctgagcctg
     5881 ggcctgaccc ccaacttcaa gagcaacttc gacctggccg aggatgccaa actgcagctg
     5941 agcaaggaca cctacgacga cgacctggac aacctgctgg cccagatcgg cgaccagtac
     6001 gccgacctgt ttctggccgc caagaacctg tccgacgcca tcctgctgag cgacatcctg
     6061 agagtgaaca ccgagatcac caaggccccc ctgagcgcct ctatgatcaa gagatacgac
     6121 gagcaccacc aggacctgac cctgctgaaa gctctcgtgc ggcagcagct gcctgagaag
     6181 tacaaagaga ttttcttcga ccagagcaag aacggctacg ccggctacat tgacggcgga
     6241 gccagccagg aagagttcta caagttcatc aagcccatcc tggaaaagat ggacggcacc
     6301 gaggaactgc tcgtgaagct gaacagagag gacctgctgc ggaagcagcg gaccttcgac
     6361 aacggcagca tcccccacca gatccacctg ggagagctgc acgccattct gcggcggcag
     6421 gaagattttt acccattcct gaaggacaac cgggaaaaga tcgagaagat cctgaccttc
     6481 cgcatcccct actacgtggg ccctctggcc aggggaaaca gcagattcgc ctggatgacc
     6541 agaaagagcg aggaaaccat caccccctgg aacttcgagg aagtggtgga caagggcgct
     6601 tccgcccaga gcttcatcga gcggatgacc aacttcgata agaacctgcc caacgagaag
     6661 gtgctgccca agcacagcct gctgtacgag tacttcaccg tgtataacga gctgaccaaa
     6721 gtgaaatacg tgaccgaggg aatgagaaag cccgccttcc tgagcggcga gcagaaaaag
     6781 gccatcgtgg acctgctgtt caagaccaac cggaaagtga ccgtgaagca gctgaaagag
     6841 gactacttca agaaaatcga gtgcttcgac tccgtggaaa tctccggcgt ggaagatcgg
     6901 ttcaacgcct ccctgggcac ataccacgat ctgctgaaaa ttatcaagga caaggacttc
     6961 ctggacaatg aggaaaacga ggacattctg gaagatatcg tgctgaccct gacactgttt
     7021 gaggacagag agatgatcga ggaacggctg aaaacctatg cccacctgtt cgacgacaaa
     7081 gtgatgaagc agctgaagcg gcggagatac accggctggg gcaggctgag ccggaagctg
     7141 atcaacggca tccgggacaa gcagtccggc aagacaatcc tggatttcct gaagtccgac
     7201 ggcttcgcca acagaaactt catgcagctg atccacgacg acagcctgac ctttaaagag
     7261 gacatccaga aagcccaggt gtccggccag ggcgatagcc tgcacgagca cattgccaat
     7321 ctggccggca gccccgccat taagaagggc atcctgcaga cagtgaaggt ggtggacgag
     7381 ctcgtgaaag tgatgggccg gcacaagccc gagaacatcg tgatcgaaat ggccagagag
     7441 aaccagacca cccagaaggg acagaagaac agccgcgaga gaatgaagcg gatcgaagag
     7501 ggcatcaaag agctgggcag ccagatcctg aaagaacacc ccgtggaaaa cacccagctg
     7561 cagaacgaga agctgtacct gtactacctg cagaatgggc gggatatgta cgtggaccag
     7621 gaactggaca tcaaccggct gtccgactac gatgtggacc atatcgtgcc tcagagcttt
     7681 ctgaaggacg actccatcga caacaaggtg ctgaccagaa gcgacaagaa ccggggcaag
     7741 agcgacaacg tgccctccga agaggtcgtg aagaagatga agaactactg gcggcagctg
     7801 ctgaacgcca agctgattac ccagagaaag ttcgacaatc tgaccaaggc cgagagaggc
     7861 ggcctgagcg aactggataa ggccggcttc atcaagagac agctggtgga aacccggcag
     7921 atcacaaagc acgtggcaca gatcctggac tcccggatga acactaagta cgacgagaat
     7981 gacaagctga tccgggaagt gaaagtgatc accctgaagt ccaagctggt gtccgatttc
     8041 cggaaggatt tccagtttta caaagtgcgc gagatcaaca actaccacca cgcccacgac
     8101 gcctacctga acgccgtcgt gggaaccgcc ctgatcaaaa agtaccctaa gctggaaagc
     8161 gagttcgtgt acggcgacta caaggtgtac gacgtgcgga agatgatcgc caagagcgag
     8221 caggaaatcg gcaaggctac cgccaagtac ttcttctaca gcaacatcat gaactttttc
     8281 aagaccgaga ttaccctggc caacggcgag atccggaagc ggcctctgat cgagacaaac
     8341 ggcgaaaccg gggagatcgt gtgggataag ggccgggatt ttgccaccgt gcggaaagtg
     8401 ctgagcatgc cccaagtgaa tatcgtgaaa aagaccgagg tgcagacagg cggcttcagc
     8461 aaagagtcta tcctgcccaa gaggaacagc gataagctga tcgccagaaa gaaggactgg
     8521 gaccctaaga agtacggcgg cttcgacagc cccaccgtgg cctattctgt gctggtggtg
     8581 gccaaagtgg aaaagggcaa gtccaagaaa ctgaagagtg tgaaagagct gctggggatc
     8641 accatcatgg aaagaagcag cttcgagaag aatcccatcg actttctgga agccaagggc
     8701 tacaaagaag tgaaaaagga cctgatcatc aagctgccta agtactccct gttcgagctg
     8761 gaaaacggcc ggaagagaat gctggcctct gccggcgaac tgcagaaggg aaacgaactg
     8821 gccctgccct ccaaatatgt gaacttcctg tacctggcca gccactatga gaagctgaag
     8881 ggctcccccg aggataatga gcagaaacag ctgtttgtgg aacagcacaa gcactacctg
     8941 gacgagatca tcgagcagat cagcgagttc tccaagagag tgatcctggc cgacgctaat
     9001 ctggacaaag tgctgtccgc ctacaacaag caccgggata agcccatcag agagcaggcc
     9061 gagaatatca tccacctgtt taccctgacc aatctgggag cccctgccgc cttcaagtac
     9121 tttgacacca ccatcgaccg gaagaggtac accagcacca aagaggtgct ggacgccacc
     9181 ctgatccacc agagcatcac cggcctgtac gagacacgga tcgacctgtc tcagctggga
     9241 ggcgacaaaa ggccggcggc cacgaaaaag gccggccagg caaaaaagaa aaagggatcc
     9301 taagctcgct ttcttgctgt ccaatttcta ttaaaggttc ctttgttccc taagtccaac
     9361 tactaaactg ggggatatta tgaagggcct tgagcatctg gattctgcct aataaaaaac
     9421 atttattttc attgctagct cgctttcttg ctgtccaatt tctattaaag gttcctttgt
     9481 tccctaagtc caactactaa actgggggat attatgaagg gccttgagca tctggattct
     9541 gcctaataaa aaacatttat tttcattgcg aattctagaa aattcctaga gctcgctgat
     9601 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
//
