LOCUS       sequence-259542-        9617 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector plasmid expressing ABEmax and sgRNA scaffold with com 
            replacing the Tetraloop..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9617)
  AUTHORS   Lyu P, Lu Z, Cho SI, Yadav M, Yoo KW, Atala A, Kim JS, Lu B
  TITLE     Adenine Base Editor Ribonucleoproteins Delivered by Lentivirus-Like 
            Particles Show High On-Target Base Editing and Undetectable RNA 
            Off-Target Activities.
  JOURNAL   CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1089/crispr.2020.0095.
  PUBMED    33616436
REFERENCE   2  (bases 1 to 9617)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/crispr.2020"; journalName: "CRISPR J"; date: "2021-02"; 
            volume: "4"; issue: "1"; pages: "69-81"
COMMENT     Sequence Label: pspCas9-ABE-3'UTR-sgRNA-Tetra-com-vector
FEATURES             Location/Qualifiers
     source          1..9617
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      187..642
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(274..293)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     484..505
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(659..678)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     778..800
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(838..856)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        924..1028
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1029..1097,1098..1889)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1247..1266)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2060..2648
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2549..2568
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2710..2958
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2710..2730
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2881..2900
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     enhancer        3098..3383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        3384..3587
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3538..3558
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3629..3648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3629..3647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3697..3717
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(4906..4929,4930..4932,4933..9006)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4906..9006
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             9007..9054
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(9364..9381)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9370..9577
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   join(9589..9617,1..112)
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   join(9589..9617,1..101)
                     /label=AAV2 ITR
ORIGIN
        1 ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg
       61 ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg
      121 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac
      181 catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
      241 tgaccgctac acttgccagc gccttagcgc ccgctccttt cgctttcttc ccttcctttc
      301 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
      361 gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta
      421 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
      481 atagtggact cttgttccaa actggaacaa cactcaactc tatctcgggc tattcttttg
      541 atttataagg gattttgccg atttcggtct attggttaaa aaatgagctg atttaacaaa
      601 aatttaacgc gaattttaac aaaatattaa cgtttacaat tttatggtgc actctcagta
      661 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
      721 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
      781 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
      841 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
      901 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
      961 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     1021 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     1081 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     1141 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     1201 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     1261 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     1321 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     1381 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     1441 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     1501 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     1561 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     1621 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     1681 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     1741 gtggaagccg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     1801 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     1861 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1921 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1981 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     2041 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     2101 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     2161 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     2221 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     2281 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     2341 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     2401 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     2461 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     2521 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     2581 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     2641 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     2701 ctcacatgtg agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct
     2761 gttagagaga taattggaat taatttgact gtaaacacaa agatattagt acaaaatacg
     2821 tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg
     2881 gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg
     2941 tggaaaggac gaaacaccgg tgtcttcctc gaggaagacc cgtttgagag ctaggccctg
     3001 aatgcctgcg agcatcccac ggcctagcaa gttcaaataa ggctagtccg ttatcaactt
     3061 gaaaaagtgg caccgagtcg gtgctttttt tggtacccgt tacataactt acggtaaatg
     3121 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaatagta acgccaatag
     3181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     3241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     3301 cctggcattg tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     3361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
     3421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     3481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     3541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctggt ttagtgaacc
     3601 gtcagatccg ctagagatcc gcggccgcta atacgactca ctatagggag agccgccacc
     3661 atgaaacgga cagccgacgg aagcgagttc gagtcaccaa agaagaagcg gaaagtctct
     3721 gaagtcgagt ttagccacga gtattggatg aggcacgcac tgaccctggc aaagcgagca
     3781 tgggatgaaa gagaagtccc cgtgggcgcc gtgctggtgc acaacaatag agtgatcgga
     3841 gagggatgga acaggccaat cggccgccac gaccctaccg cacacgcaga gatcatggca
     3901 ctgaggcagg gaggcctggt catgcagaat taccgcctga tcgatgccac cctgtatgtg
     3961 acactggagc catgcgtgat gtgcgcagga gcaatgatcc acagcaggat cggaagagtg
     4021 gtgttcggag cacgggacgc caagaccggc gcagcaggct ccctgatgga tgtgctgcac
     4081 caccccggca tgaaccaccg ggtggagatc acagagggaa tcctggcaga cgagtgcgcc
     4141 gccctgctga gcgatttctt tagaatgcgg agacaggaga tcaaggccca gaagaaggca
     4201 cagagctcca ccgactctgg aggatctagc ggaggatcct ctggaagcga gacaccaggc
     4261 acaagcgagt ccgccacacc agagagctcc ggcggctcct ccggaggatc ctctgaggtg
     4321 gagttttccc acgagtactg gatgagacat gccctgaccc tggccaagag ggcacgcgat
     4381 gagagggagg tgcctgtggg agccgtgctg gtgctgaaca atagagtgat cggcgagggc
     4441 tggaacagag ccatcggcct gcacgaccca acagcccatg ccgaaattat ggccctgaga
     4501 cagggcggcc tggtcatgca gaactacaga ctgattgacg ccaccctgta cgtgacattc
     4561 gagccttgcg tgatgtgcgc cggcgccatg atccactcta ggatcggccg cgtggtgttt
     4621 ggcgtgagga acgcaaaaac cggcgccgca ggctccctga tggacgtgct gcactacccc
     4681 ggcatgaatc accgcgtcga aattaccgag ggaatcctgg cagatgaatg tgccgccctg
     4741 ctgtgctatt tctttcggat gcctagacag gtgttcaatg ctcagaagaa ggcccagagc
     4801 tccaccgact ccggaggatc tagcggaggc tcctctggct ctgagacacc tggcacaagc
     4861 gagagcgcaa cacctgaaag cagcgggggc agcagcgggg ggtcagacaa gaagtacagc
     4921 atcggcctgg ccatcggcac caactctgtg ggctgggccg tgatcaccga cgagtacaag
     4981 gtgcccagca agaaattcaa ggtgctgggc aacaccgacc ggcacagcat caagaagaac
     5041 ctgatcggag ccctgctgtt cgacagcggc gaaacagccg aggccacccg gctgaagaga
     5101 accgccagaa gaagatacac cagacggaag aaccggatct gctatctgca agagatcttc
     5161 agcaacgaga tggccaaggt ggacgacagc ttcttccaca gactggaaga gtccttcctg
     5221 gtggaagagg ataagaagca cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg
     5281 gcctaccacg agaagtaccc caccatctac cacctgagaa agaaactggt ggacagcacc
     5341 gacaaggccg acctgcggct gatctatctg gccctggccc acatgatcaa gttccggggc
     5401 cacttcctga tcgagggcga cctgaacccc gacaacagcg acgtggacaa gctgttcatc
     5461 cagctggtgc agacctacaa ccagctgttc gaggaaaacc ccatcaacgc cagcggcgtg
     5521 gacgccaagg ccatcctgtc tgccagactg agcaagagca gacggctgga aaatctgatc
     5581 gcccagctgc ccggcgagaa gaagaatggc ctgttcggaa acctgattgc cctgagcctg
     5641 ggcctgaccc ccaacttcaa gagcaacttc gacctggccg aggatgccaa actgcagctg
     5701 agcaaggaca cctacgacga cgacctggac aacctgctgg cccagatcgg cgaccagtac
     5761 gccgacctgt ttctggccgc caagaacctg tccgacgcca tcctgctgag cgacatcctg
     5821 agagtgaaca ccgagatcac caaggccccc ctgagcgcct ctatgatcaa gagatacgac
     5881 gagcaccacc aggacctgac cctgctgaaa gctctcgtgc ggcagcagct gcctgagaag
     5941 tacaaagaga ttttcttcga ccagagcaag aacggctacg ccggctacat tgacggcgga
     6001 gccagccagg aagagttcta caagttcatc aagcccatcc tggaaaagat ggacggcacc
     6061 gaggaactgc tcgtgaagct gaacagagag gacctgctgc ggaagcagcg gaccttcgac
     6121 aacggcagca tcccccacca gatccacctg ggagagctgc acgccattct gcggcggcag
     6181 gaagattttt acccattcct gaaggacaac cgggaaaaga tcgagaagat cctgaccttc
     6241 cgcatcccct actacgtggg ccctctggcc aggggaaaca gcagattcgc ctggatgacc
     6301 agaaagagcg aggaaaccat caccccctgg aacttcgagg aagtggtgga caagggcgct
     6361 tccgcccaga gcttcatcga gcggatgacc aacttcgata agaacctgcc caacgagaag
     6421 gtgctgccca agcacagcct gctgtacgag tacttcaccg tgtataacga gctgaccaaa
     6481 gtgaaatacg tgaccgaggg aatgagaaag cccgccttcc tgagcggcga gcagaaaaag
     6541 gccatcgtgg acctgctgtt caagaccaac cggaaagtga ccgtgaagca gctgaaagag
     6601 gactacttca agaaaatcga gtgcttcgac tccgtggaaa tctccggcgt ggaagatcgg
     6661 ttcaacgcct ccctgggcac ataccacgat ctgctgaaaa ttatcaagga caaggacttc
     6721 ctggacaatg aggaaaacga ggacattctg gaagatatcg tgctgaccct gacactgttt
     6781 gaggacagag agatgatcga ggaacggctg aaaacctatg cccacctgtt cgacgacaaa
     6841 gtgatgaagc agctgaagcg gcggagatac accggctggg gcaggctgag ccggaagctg
     6901 atcaacggca tccgggacaa gcagtccggc aagacaatcc tggatttcct gaagtccgac
     6961 ggcttcgcca acagaaactt catgcagctg atccacgacg acagcctgac ctttaaagag
     7021 gacatccaga aagcccaggt gtccggccag ggcgatagcc tgcacgagca cattgccaat
     7081 ctggccggca gccccgccat taagaagggc atcctgcaga cagtgaaggt ggtggacgag
     7141 ctcgtgaaag tgatgggccg gcacaagccc gagaacatcg tgatcgaaat ggccagagag
     7201 aaccagacca cccagaaggg acagaagaac agccgcgaga gaatgaagcg gatcgaagag
     7261 ggcatcaaag agctgggcag ccagatcctg aaagaacacc ccgtggaaaa cacccagctg
     7321 cagaacgaga agctgtacct gtactacctg cagaatgggc gggatatgta cgtggaccag
     7381 gaactggaca tcaaccggct gtccgactac gatgtggacc atatcgtgcc tcagagcttt
     7441 ctgaaggacg actccatcga caacaaggtg ctgaccagaa gcgacaagaa ccggggcaag
     7501 agcgacaacg tgccctccga agaggtcgtg aagaagatga agaactactg gcggcagctg
     7561 ctgaacgcca agctgattac ccagagaaag ttcgacaatc tgaccaaggc cgagagaggc
     7621 ggcctgagcg aactggataa ggccggcttc atcaagagac agctggtgga aacccggcag
     7681 atcacaaagc acgtggcaca gatcctggac tcccggatga acactaagta cgacgagaat
     7741 gacaagctga tccgggaagt gaaagtgatc accctgaagt ccaagctggt gtccgatttc
     7801 cggaaggatt tccagtttta caaagtgcgc gagatcaaca actaccacca cgcccacgac
     7861 gcctacctga acgccgtcgt gggaaccgcc ctgatcaaaa agtaccctaa gctggaaagc
     7921 gagttcgtgt acggcgacta caaggtgtac gacgtgcgga agatgatcgc caagagcgag
     7981 caggaaatcg gcaaggctac cgccaagtac ttcttctaca gcaacatcat gaactttttc
     8041 aagaccgaga ttaccctggc caacggcgag atccggaagc ggcctctgat cgagacaaac
     8101 ggcgaaaccg gggagatcgt gtgggataag ggccgggatt ttgccaccgt gcggaaagtg
     8161 ctgagcatgc cccaagtgaa tatcgtgaaa aagaccgagg tgcagacagg cggcttcagc
     8221 aaagagtcta tcctgcccaa gaggaacagc gataagctga tcgccagaaa gaaggactgg
     8281 gaccctaaga agtacggcgg cttcgacagc cccaccgtgg cctattctgt gctggtggtg
     8341 gccaaagtgg aaaagggcaa gtccaagaaa ctgaagagtg tgaaagagct gctggggatc
     8401 accatcatgg aaagaagcag cttcgagaag aatcccatcg actttctgga agccaagggc
     8461 tacaaagaag tgaaaaagga cctgatcatc aagctgccta agtactccct gttcgagctg
     8521 gaaaacggcc ggaagagaat gctggcctct gccggcgaac tgcagaaggg aaacgaactg
     8581 gccctgccct ccaaatatgt gaacttcctg tacctggcca gccactatga gaagctgaag
     8641 ggctcccccg aggataatga gcagaaacag ctgtttgtgg aacagcacaa gcactacctg
     8701 gacgagatca tcgagcagat cagcgagttc tccaagagag tgatcctggc cgacgctaat
     8761 ctggacaaag tgctgtccgc ctacaacaag caccgggata agcccatcag agagcaggcc
     8821 gagaatatca tccacctgtt taccctgacc aatctgggag cccctgccgc cttcaagtac
     8881 tttgacacca ccatcgaccg gaagaggtac accagcacca aagaggtgct ggacgccacc
     8941 ctgatccacc agagcatcac cggcctgtac gagacacgga tcgacctgtc tcagctggga
     9001 ggcgacaaaa ggccggcggc cacgaaaaag gccggccagg caaaaaagaa aaagggatcc
     9061 taagctcgct ttcttgctgt ccaatttcta ttaaaggttc ctttgttccc taagtccaac
     9121 tactaaactg ggggatatta tgaagggcct tgagcatctg gattctgcct aataaaaaac
     9181 atttattttc attgctagct cgctttcttg ctgtccaatt tctattaaag gttcctttgt
     9241 tccctaagtc caactactaa actgggggat attatgaagg gccttgagca tctggattct
     9301 gcctaataaa aaacatttat tttcattgcg aattctagaa aattcctaga gctcgctgat
     9361 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     9421 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     9481 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     9541 gggaggattg ggaagagaat agcaggcatg ctggggaggt acggccgcag gaacccctag
     9601 tgatggagtt ggccact
//
