LOCUS       sequence-259550-        8774 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a TurboGFP transcript with microRNA sponge for 
            mmu-miR-16-5p as 3'UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8774)
  TITLE     Ciaudo Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCW57.1-GFP_mmu-miR-16-5p_sponge
FEATURES             Location/Qualifiers
     source          1..8774
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..320
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    14..32
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    50..68
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    85..103
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    121..139
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    157..175
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    192..210
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    228..246
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     293..317
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      343..352
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             349..1044
                     /codon_start=1
                     /product="improved green fluorescent protein from 
                     Pontellina plumata (Evdokimov et al., 2006)"
                     /label=TurboGFP
                     /note="mammalian codon-optimized"
                     /translation="MESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKM
                     KSTKGALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVL
                     HVSFSYRYEAGRVIGDFKVMGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTR
                     TFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFAFRRVEEDHSNTELGIVEYQHAF
                     KTPDADAGEE"
     polyA_signal    1367..1501
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        1510..2020
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     1939..1957
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2030..2626
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2030..2049)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2526..2546
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             2627..2680
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(2681..3298,3299..3304,3305..3340,3341..3343,3344..3379,
                     3380..3382,3383..3418,3419..3427)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(3467..3487)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3885..3896)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4101..4334
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4406..4527
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      4567..4702
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4702..4773
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4774..4842,4843..5634)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4992..5011)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5805..6393
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6294..6313
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6547..6564
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6681..6702
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6717..6722,6723..6740,6741..6747)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6755..6771
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6760..6782
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6779..6795
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6779..6795
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6814..6834
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6816..6834
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6862..7088
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7089..7269
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7316..7441
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7934..8167
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8352..8396
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8609..8726
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt
       61 gatagagaac gatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttac
      121 tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta
      181 tgtcgagttt atccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga
      241 tagagaacgt atgtcgaggt aggcgtgtac ggtgggaggc ctatataagc agagctcgtt
      301 tagtgaaccg tcagatcgcc tggagaattg gctagcgaat tcgccaccat ggagagcgac
      361 gagagcggcc tgcccgccat ggagatcgag tgccgcatca ccggcaccct gaacggcgtg
      421 gagttcgagc tggtgggcgg cggagagggc acccccgagc agggccgcat gaccaacaag
      481 atgaagagca ccaaaggcgc cctgaccttc agcccctacc tgctgagcca cgtgatgggc
      541 tacggcttct accacttcgg cacctacccc agcggctacg agaacccctt cctgcacgcc
      601 atcaacaacg gcggctacac caacacccgc atcgagaagt acgaggacgg cggcgtgctg
      661 cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt caaggtgatg
      721 ggcaccggct tccccgagga cagcgtgatc ttcaccgaca agatcatccg cagcaacgcc
      781 accgtggagc acctgcaccc catgggcgat aacgatctgg atggcagctt cacccgcacc
      841 ttcagcctgc gcgacggcgg ctactacagc tccgtggtgg acagccacat gcacttcaag
      901 agcgccatcc accccagcat cctgcagaac gggggcccca tgttcgcctt ccgccgcgtg
      961 gaggaggatc acagcaacac cgagctgggc atcgtggagt accagcacgc cttcaagacc
     1021 ccggatgcag atgccggtga agaaaccggt tagcgccaat attccttgct gctaactgcg
     1081 ccaatattcc ttgctgctaa ctgcgccaat attccttgct gctaactgcg ccaatattcc
     1141 ttgctgctaa ctgcgccaat attccttgct gctaactgcg ccaatattcc ttgctgctaa
     1201 ctgcgccaat attccttgct gctaactgcg ccaatattcc ttgctgctaa ctgcgccaat
     1261 attccttgct gctaactgcg ccaatattcc ttgctgctaa ctgcgccaat attccttgct
     1321 gctaactgcg ccaatattcc ttgctgctat taattaacca tggcccaact tgtttattgc
     1381 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     1441 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
     1501 ctcggatccg gggttggggt tgcgcctttt ccaaggcagc cctgggtttg cgcagggacg
     1561 cggctgctct gggcgtggtt ccgggaaacg cagcggcgcc gaccctgggt ctcgcacatt
     1621 cttcacgtcc gttcgcagcg tcacccggat cttcgccgct acccttgtgg gccccccggc
     1681 gacgcttcct gctccgcccc taagtcggga aggttccttg cggttcgcgg cgtgccggac
     1741 gtgacaaacg gaagccgcac gtctcactag taccctcgca gacggacagc gccagggagc
     1801 aatggcagcg cgccgaccgc gatgggctgt ggccaatagc ggctgctcag cagggcgcgc
     1861 cgagagcagc ggccgggaag gggcggtgcg ggaggcgggg tgtggggcgg tagtgtgggc
     1921 cctgttcctg cccgcgcggt gttccgcatt ctgcaagcct ccggagcgca cgtcggcagt
     1981 cggctccctc gttgaccgaa tcaccgacct ctctccccag caattcacca tgaccgagta
     2041 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
     2101 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
     2161 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     2221 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
     2281 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     2341 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     2401 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     2461 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     2521 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     2581 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgccgaag gtagaggttc
     2641 tctcctcact tgtggtgatg ttgaagaaaa ccctggtcca atgtctagac tggacaagag
     2701 caaagtcata aacggagctc tggaattact caatggtgtc ggtatcgaag gcctgacgac
     2761 aaggaaactc gctcaaaagc tgggagttga gcagcctacc ctgtactggc acgtgaagaa
     2821 caagcgggcc ctgctcgatg ccctgccaat cgagatgctg gacaggcatc atacccactt
     2881 ctgccccctg gaaggcgagt catggcaaga ctttctgcgg aacaacgcca agtcataccg
     2941 ctgtgctctc ctctcacatc gcgacggggc taaagtgcat ctcggcaccc gcccaacaga
     3001 gaaacagtac gaaaccctgg aaaatcagct cgcgttcctg tgtcagcaag gcttctccct
     3061 ggagaacgca ctgtacgctc tgtccgccgt gggccacttt acactgggct gcgtattgga
     3121 ggaacaggag catcaagtag caaaagagga aagagagaca cctaccaccg attctatgcc
     3181 cccacttctg agacaagcaa ttgagctgtt cgaccggcag ggagccgaac ctgccttcct
     3241 tttcggcctg gaactaatca tatgtggcct ggagaaacag ctaaagtgcg aaagcggcgg
     3301 gccgaccgac gcccttgacg attttgactt agacatgctc ccagccgatg cccttgacga
     3361 ctttgacctt gatatgctgc ctgctgacgc tcttgacgat tttgaccttg acatgctccc
     3421 cgggtaacta agtaaggatc gatccaagat atcgtattct taactatgtt gctcctttta
     3481 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     3541 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     3601 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     3661 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     3721 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     3781 ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg
     3841 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     3901 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     3961 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctgtttcg cctcggcgtc
     4021 cggactagag gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     4081 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct
     4141 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     4201 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     4261 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     4321 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     4381 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata
     4441 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     4501 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact
     4561 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     4621 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     4681 tgaggaggct tttttggagg cctttcaaat atgtatccgc tcatgagaca ataaccctga
     4741 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     4801 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     4861 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     4921 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     4981 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     5041 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     5101 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     5161 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     5221 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     5281 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     5341 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     5401 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     5461 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     5521 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     5581 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     5641 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     5701 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     5761 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     5821 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     5881 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     5941 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     6001 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     6061 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     6121 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     6181 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     6241 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     6301 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     6361 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     6421 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     6481 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     6541 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     6601 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     6661 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     6721 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     6781 ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa
     6841 aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg
     6901 taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg
     6961 tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga
     7021 ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat
     7081 acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     7141 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     7201 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     7261 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     7321 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     7381 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     7441 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     7501 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     7561 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     7621 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     7681 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     7741 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg
     7801 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     7861 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     7921 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     7981 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     8041 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     8101 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     8161 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     8221 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     8281 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     8341 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     8401 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     8461 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     8521 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggacaa ttctcgacct
     8581 cgagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca
     8641 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa
     8701 aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga gatccacttt
     8761 ggccgcgaat cgat
//
