LOCUS       sequence-259554-        8921 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a TurboRFP transcript with test microRNA sponge for 
            mmu-miR-292b-3p as 3'UTR; serves as template for other microRNA 
            sponges.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8921)
  TITLE     Ciaudo Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCW57.1-RFP_mmu-miR-292b-3p_sponge
FEATURES             Location/Qualifiers
     source          1..8921
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    41..158
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        212..526
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    220..238
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    256..274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    291..309
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    327..345
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    363..381
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    398..416
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    434..452
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     499..523
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             555..1247
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
     polyA_signal    1486..1620
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        1629..2139
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2058..2076
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             join(2163..2780,2781..2786,2787..2822,2823..2825,2826..2861,
                     2862..2864,2865..2900,2901..2906)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             2922..2975
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2976..3779
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MSRMIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3039..3058)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3649..3668
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(3820..3840)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4238..4249)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4454..4687
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4759..4880
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      4920..5055
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5055..5126
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5127..5195,5196..5987)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5345..5364)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6158..6746
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6647..6666
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6900..6917
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7034..7055
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7070..7075,7076..7093,7094..7100)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7108..7124
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7113..7135
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7132..7148
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7132..7148
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7167..7187
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7169..7187
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7215..7441
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7442..7622
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7669..7794
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8287..8520
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8705..8749
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
       61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      181 gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt gatagagaac
      241 gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag
      301 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
      361 actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt
      421 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg
      481 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattggcta
      541 gcgaattcgc caccatgagc gagctgatca aggagaacat gcacatgaag ctgtacatgg
      601 agggcaccgt gaacaaccac cacttcaagt gcacatccga gggcgaaggc aagccctacg
      661 agggcaccca gaccatgaag atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg
      721 acatcctggc taccagcttc atgtacggca gcaaagcctt catcaaccac acccagggca
      781 tccccgactt ctttaagcag tccttccctg agggcttcac atgggagaga atcaccacat
      841 acgaagacgg gggcgtgctg accgctaccc aggacaccag cttccagaac ggctgcatca
      901 tctacaacgt caagatcaac ggggtgaact tcccatccaa cggccctgtg atgcagaaga
      961 aaacacgcgg ctgggaggcc aacaccgaga tgctgtaccc cgctgacggc ggcctgagag
     1021 gccacagcca gatggccctg aagctcgtgg gcgggggcta cctgcactgc tccttcaaga
     1081 ccacatacag atccaagaaa cccgctaaga acctcaagat gcccggcttc cacttcgtgg
     1141 accacagact ggaaagaatc aaggaggccg acaaagagac ctacgtcgag cagcacgaga
     1201 tggctgtggc caagtactgc gacctcccta gcaaactggg gcacagaacc ggttagatac
     1261 tcaaagatgg gctcttactg atactcaaag atgggctctt actgatactc aaagatgggc
     1321 tcttactgat actcaaagat gggctcttac tgatactcaa agatgggctc ttactgatac
     1381 tcaaagatgg gctcttactg atactcaaag atgggctctt actgatactc aaagatgggc
     1441 tcttactgat actcaaagat gggctctttt aattaaccat ggcccaactt gtttattgca
     1501 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     1561 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
     1621 tcggatccgg ggttggggtt gcgccttttc caaggcagcc ctgggtttgc gcagggacgc
     1681 ggctgctctg ggcgtggttc cgggaaacgc agcggcgccg accctgggtc tcgcacattc
     1741 ttcacgtccg ttcgcagcgt cacccggatc ttcgccgcta cccttgtggg ccccccggcg
     1801 acgcttcctg ctccgcccct aagtcgggaa ggttccttgc ggttcgcggc gtgccggacg
     1861 tgacaaacgg aagccgcacg tctcactagt accctcgcag acggacagcg ccagggagca
     1921 atggcagcgc gccgaccgcg atgggctgtg gccaatagcg gctgctcagc agggcgcgcc
     1981 gagagcagcg gccgggaagg ggcggtgcgg gaggcggggt gtggggcggt agtgtgggcc
     2041 ctgttcctgc ccgcgcggtg ttccgcattc tgcaagcctc cggagcgcac gtcggcagtc
     2101 ggctccctcg ttgaccgaat caccgacctc tctccccagc aattcacccg cgcgcggcca
     2161 ccatgtctag actggacaag agcaaagtca taaacggagc tctggaatta ctcaatggtg
     2221 tcggtatcga aggcctgacg acaaggaaac tcgctcaaaa gctgggagtt gagcagccta
     2281 ccctgtactg gcacgtgaag aacaagcggg ccctgctcga tgccctgcca atcgagatgc
     2341 tggacaggca tcatacccac ttctgccccc tggaaggcga gtcatggcaa gactttctgc
     2401 ggaacaacgc caagtcatac cgctgtgctc tcctctcaca tcgcgacggg gctaaagtgc
     2461 atctcggcac ccgcccaaca gagaaacagt acgaaaccct ggaaaatcag ctcgcgttcc
     2521 tgtgtcagca aggcttctcc ctggagaacg cactgtacgc tctgtccgcc gtgggccact
     2581 ttacactggg ctgcgtattg gaggaacagg agcatcaagt agcaaaagag gaaagagaga
     2641 cacctaccac cgattctatg cccccacttc tgagacaagc aattgagctg ttcgaccggc
     2701 agggagccga acctgccttc cttttcggcc tggaactaat catatgtggc ctggagaaac
     2761 agctaaagtg cgaaagcggc gggccgaccg acgcccttga cgattttgac ttagacatgc
     2821 tcccagccga tgcccttgac gactttgacc ttgatatgct gcctgctgac gctcttgacg
     2881 attttgacct tgacatgctc cccgggttcg aaggaagcgg agaaggtaga ggttctctcc
     2941 tcacttgtgg tgatgttgaa gaaaaccctg gtccaatgtc tagaatgatt gaacaagatg
     3001 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
     3061 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
     3121 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc
     3181 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
     3241 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
     3301 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
     3361 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
     3421 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
     3481 cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg
     3541 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
     3601 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
     3661 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
     3721 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgaa
     3781 tgcattgagg atcgatccaa gatatcgtat tcttaactat gttgctcctt ttacgctatg
     3841 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     3901 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     3961 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     4021 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     4081 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     4141 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
     4201 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     4261 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     4321 gacgagtcgg atctcccttt gggccgcctc cccgcctgtt tcgcctcggc gtccggacta
     4381 gaggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     4441 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct
     4501 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     4561 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     4621 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     4681 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     4741 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     4801 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     4861 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
     4921 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     4981 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     5041 gcttttttgg aggcctttca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     5101 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     5161 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     5221 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     5281 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     5341 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     5401 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     5461 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     5521 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     5581 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     5641 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     5701 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     5761 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     5821 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     5881 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     5941 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     6001 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     6061 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     6121 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     6181 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     6241 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     6301 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     6361 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     6421 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     6481 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     6541 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     6601 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     6661 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     6721 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     6781 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     6841 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     6901 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     6961 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     7021 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     7081 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     7141 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
     7201 agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat
     7261 gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt
     7321 aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg
     7381 aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa
     7441 cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     7501 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     7561 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     7621 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     7681 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     7741 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
     7801 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     7861 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     7921 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     7981 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     8041 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     8101 caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg
     8161 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     8221 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     8281 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     8341 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     8401 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     8461 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     8521 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     8581 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     8641 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
     8701 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
     8761 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
     8821 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
     8881 accattatcg tttcagaccc acctcccaac cccgagggga c
//
