LOCUS       sequence-25956-e         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral eGFP expression vector, PGK promoter, Blast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pLenti PGK GFP Blast (w510-5)
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      24..33
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(49..559)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(112..130)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     misc_feature    605..722
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ccggtaagct ctgcccttgc tcaccatggt ggcgaccggt ggatccccct ggggagagag
       61 gtcggtgatt cggtcaacga gggagccgac tgccgacgtg cgctccggag gcttgcagaa
      121 tgcggaacac cgcgcgggca ggaacagggc ccacactacc gccccacacc ccgcctcccg
      181 caccgcccct tcccggccgc tgctctcggc gcgccctgct gagcagccgc tattggccac
      241 agcccatcgc ggtcggcgcg ctgccattgc tccctggcgc tgtccgtctg cgagggtact
      301 agtgagacgt gcggcttccg tttgtcacgt ccggcacgcc gcgaaccgca aggaaccttc
      361 ccgacttagg ggcggagcag gaagcgtcgc cggggggccc acaagggtag cggcgaagat
      421 ccgggtgacg ctgcgaacgg acgtgaagaa tgtgcgagac ccagggtcgg cgccgctgcg
      481 tttcccggaa ccacgcccag agcagccgcg tccctgcgca aacccagggc tgccttggaa
      541 aaggcgcaac cccaaccccg tggaattcga tatcaagctt ctcgaggcta gtctcgtgat
      601 cgataaaatt ttgaattttt gtaatttgtt tttgtaattc tttagtttgt atgtctgttg
      661 ctattatgtc tactattctt tcccctgcac tgtacccccc aatcccccct tttcttttaa
      721 aagttaaccg ataccgtcga gatccgttca ctaatcgaat ggatctgtct ctgtctctct
      781 ctccacctt
//
